Abstract:
:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the beta-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused beta-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH. Unlike beta-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv Hdoi
10.1093/nar/9.1.19subject
Has Abstractpub_date
1981-01-10 00:00:00pages
19-30issue
1eissn
0305-1048issn
1362-4962journal_volume
9pub_type
杂志文章abstract::The p-n-butylphenyl- and p-n-butylanilino- substituted analogs of dGTP and dATP, respectively, were tested as inhibitors of purified human placental DNA polymerases alpha and delta. It was observed that DNA polymerase alpha activity was potently inhibited by these analogs with I0.5 values as low as the nanomolar range...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.23.8623
更新日期:1985-12-09 00:00:00
abstract::Decay of (125)I produces a shower of low energy electrons (Auger electrons) that cause strand breaks in DNA in a distance-dependent manner with 90% of the breaks located within 10 bp from the decay site. We studied strand breaks in RNA molecules produced by decay of (125)I incorporated into complementary DNA oligonucl...
journal_title:Nucleic acids research
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doi:10.1093/nar/gkf622
更新日期:2002-11-15 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gki219
更新日期:2005-01-28 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1994-10-25 00:00:00
abstract::DNA primase has been partially purified from wheat germ. This enzyme, like DNA primases characterized from many procaryotic and eucaryotic sources, catalyses the synthesis of primers involved in DNA replication. However, the wheat enzyme differs from animal DNA primase in that it is found partially associated with a D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.16.4867
更新日期:1990-08-25 00:00:00
abstract::Calf thymus nucleosomes exhibit two different and independent hydrodynamic responses to diminishing salt concentration. One change is gradual over the range 40 to 0.2 mM Na+ and is accompanied by decreases in contact-site cross-linking efficiency. The other change is abrupt, being centered between 1 and 2 mM Na+. We f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.21.4969
更新日期:1980-11-11 00:00:00
abstract::Metnase is a human protein with methylase (SET) and nuclease domains that is widely expressed, especially in proliferating tissues. Metnase promotes non-homologous end-joining (NHEJ), and knockdown causes mild hypersensitivity to ionizing radiation. Metnase also promotes plasmid and viral DNA integration, and topoisom...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq339
更新日期:2010-09-01 00:00:00
abstract::Real-time PCR is being used increasingly as the method of choice for mRNA quantification, allowing rapid analysis of gene expression from low quantities of starting template. Despite a wide range of approaches, the same principles underlie all data analysis, with standard approaches broadly classified as either absolu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng073
更新日期:2003-07-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.12.2105
更新日期:1995-06-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3722
更新日期:1996-10-01 00:00:00
abstract::Small regulatory RNAs (sRNAs) from bacterial chromosomes became the focus of research over the past five years. However, relatively little is known in terms of structural requirements, kinetics of interaction with their targets and degradation in contrast to well-studied plasmid-encoded antisense RNAs. Here, we presen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm439
更新日期:2007-01-01 00:00:00
abstract::We have examined the replication patterns of ribosomal DNA plasmids in vivo and in vitro using Xenopus eggs. Plasmids carrying different parts of the Xenopus ribosomal DNA sequence were allowed to replicate either in vitro in an egg extract or in vivo after microinjection into unfertilized eggs. The replication interm...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.7.1463
更新日期:1992-04-11 00:00:00
abstract::We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic acid binders containing a variety chemical scaffolds. We used a combination of principal c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky019
更新日期:2018-04-20 00:00:00
abstract::We determined the structure around the gap region between 28S alpha and 28S beta rRNA of Bombyx mori, a lepidopteran insect, to know the introduction mechanism of the hidden break, an interruption of the phosphodiester bond specific to the 28S rRNA of protostomes. Sequence analysis and S1 nuclease mapping suggested th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.16.6393
更新日期:1986-08-26 00:00:00
abstract::A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt763
更新日期:2013-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm067
更新日期:2007-01-01 00:00:00
abstract::Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three ger...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1096
更新日期:2016-01-04 00:00:00
abstract::We have developed a simple method to measure RNA synthesis in real time. In this technique, transcription reactions are performed in the presence of molecular beacons that possess a 2'-O-methylribonucleotide backbone. These probes become fluorescent as they hybridize to nascent RNA during the course of synthesis. We f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh068
更新日期:2004-05-20 00:00:00
abstract::RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.23.7763
更新日期:1982-12-11 00:00:00
abstract::ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F(0)F(...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg326
更新日期:2003-05-01 00:00:00
abstract::Non-protein-coding RNAs (ncRNAs) fulfill a wide range of cellular functions from protein synthesis to regulation of gene expression. Identification of novel regulatory ncRNAs by experimental approaches commonly includes the generation of specialized cDNA libraries encoding small ncRNA species. However, such identifica...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm244
更新日期:2007-01-01 00:00:00
abstract::Periodically repeating DNA and protein elements are involved in various important biological events including genomic evolution, gene regulation, protein complex formation, and immunity. Notably, the currently used genome editing tools such as ZFNs, TALENs, and CRISPRs are also all associated with periodically repeati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky890
更新日期:2019-01-25 00:00:00
abstract::We have examined the behaviour of the beta-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the beta-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the beta-interferon p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.21.4489
更新日期:1994-10-25 00:00:00
abstract::Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.11.3027
更新日期:1991-06-11 00:00:00
abstract::Sensitive detection of microsatellite instability (MSI) in tissue or liquid biopsies using next generation sequencing (NGS) has growing prognostic and predictive applications in cancer. However, the complexities of NGS make it cumbersome as compared to established multiplex-PCR detection of MSI. We present a new appro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1175
更新日期:2020-12-08 00:00:00
abstract::2-Thiouracil-containing nucleosides are essential modified units of natural and synthetic nucleic acids. In particular, the 5-substituted-2-thiouridines (S2Us) present in tRNA play an important role in tuning the translation process through codon-anticodon interactions. The enhanced thermodynamic stability of S2U-cont...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv109
更新日期:2015-03-11 00:00:00
abstract::We have generated a codon usage table for Pseudomonas aeruginosa. Codon usage in P. aeruginosa is extremely biased. In contrast to E. coli and yeast, P. aeruginosa preferentially uses those codons within a synonymous codon group with the strongest predicted codon-anticodon interaction. We were unable to correlate a pa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.19.9323
更新日期:1988-10-11 00:00:00
abstract::The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured HpaII restriction fragments. In every case an excess (1.4-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.5.1449
更新日期:1977-01-01 00:00:00
abstract::Southern blot analyses using reduced stringency hybridization conditions have been employed to search for sequence homologies between rodent VL30 genes and murine leukemia virus (MuLV) proviruses. These constitute two classes of transposon-like elements previously believed to be genetically unrelated. Our results demo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.2.305
更新日期:1983-01-25 00:00:00
abstract::Transcription is the first step in gene expression, and it is the step at which most of the regulation of expression occurs. Although sequenced prokaryotic genomes provide a wealth of information, transcriptional regulatory networks are still poorly understood using the available genomic information, largely because a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr795
更新日期:2012-02-01 00:00:00