Cloning of bovine growth hormone gene and its expression in bacteria.

Abstract:

:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the beta-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused beta-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH. Unlike beta-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv H

doi

10.1093/nar/9.1.19

subject

Has Abstract

pub_date

1981-01-10 00:00:00

pages

19-30

issue

1

eissn

0305-1048

issn

1362-4962

journal_volume

9

pub_type

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