Alternatively spliced p53 RNA in transformed and normal cells of different tissue types.

abstract：:Controllable gene delivery via vector-based systems remains a formidable challenge in mammalian synthetic biology and a desirable asset in gene therapy applications. Here, we introduce a methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fr...

journal_title：Nucleic acids research

authors： Moore R,Spinhirne A,Lai MJ,Preisser S,Li Y,Kang T,Bleris L

更新日期：2015-01-01 00:00:00

abstract：:Sequencing by hybridization (SBH) approaches to DNA sequencing face two conflicting constraints. First, in order to ensure that the target DNA binds reliably, the oligonucleotide probes that are attached to the chip array must be >15 bp in length. Secondly, the total number of possible 15 bp oligonucleotides is too la...

journal_title：Nucleic acids research

authors： Fedrigo O,Naylor G

更新日期：2004-02-18 00:00:00

abstract：:Human cytomegalovirus (HCMV) terminase is composed of subunits pUL56 (130 kDa) and pUL89 ( approximately 75 kDa), encoded by the UL56 and UL89 genes. In a recent investigation, we demonstrated that the main ATPase activity is associated with the large terminase subunit pUL56. The protein has two putative ATP-binding s...

journal_title：Nucleic acids research

authors： Scholz B,Rechter S,Drach JC,Townsend LB,Bogner E

更新日期：2003-03-01 00:00:00

abstract：:The complete nucleotide sequences of the various precursor 5S RNA species occurring in Bacillus licheniformis have been elucidated. The B. licheniformis precursors contain a 5'-precursor-specific segment of 95 nucleotides which is four times as long as the corresponding segment of the p5S RNAs from the closely related...

journal_title：Nucleic acids research

authors： Stiekema WJ,de Leede-Twente R,Raué HA,Planta RJ

更新日期：1980-10-10 00:00:00

abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...

journal_title：Nucleic acids research

authors： McLaughlin CR,Tao Q,Abood ME

更新日期：1994-12-11 00:00:00

abstract：:In Xenopus oocytes, progesterone stimulates the cytoplasmic polyadenylation and resulting translational activation of c-mos mRNA, which is necessary for the induction of oocyte maturation. Although details of the biochemistry of polyadenylation are beginning to emerge, the mechanism by which 3' poly(A) addition stimul...

journal_title：Nucleic acids research

authors： Kuge H,Brownlee GG,Gershon PD,Richter JD

更新日期：1998-07-01 00:00:00

abstract：:We have isolated a pigment cell-specific cDNA clone from a B16 mouse melanoma cDNA library by differential hybridization. The mRNA of isolated cDNA is highly expressed in B16 melanoma cells and in black mouse (C57BL/6) skin, but is not detectable in mouse neuroblastoma cells nor in K1735 mouse amelanotic melanoma cell...

journal_title：Nucleic acids research

authors： Shibahara S,Tomita Y,Sakakura T,Nager C,Chaudhuri B,Müller R

更新日期：1986-03-25 00:00:00

abstract：:MicA is a trans-encoded small non-coding RNA, which downregulates porin-expression in stationary-phase. In this work, we focus on the role of endoribonucleases III and E on Salmonella typhimurium sRNA MicA regulation. RNase III is shown to regulate MicA in a target-coupled way, while RNase E is responsible for the con...

journal_title：Nucleic acids research

authors： Viegas SC,Silva IJ,Saramago M,Domingues S,Arraiano CM

更新日期：2011-04-01 00:00:00

abstract：:Three peaks of methyltransferase activity specific for MNNG alkylated DNA have been identified from extracts of chemically adapted M. luteus. They are designated as TI to TIII in order to their elution from a Sephadex G-75 column. The first one of these peaks has been purified to homogeneity. TI, is an inducible, unus...

journal_title：Nucleic acids research

authors： Riazuddin S,Athar A,Sohail A

更新日期：1987-11-25 00:00:00

abstract：:A detailed scheme of the Peptidyl Transferase Centre of bacterial ribosomes is proposed by summarizing the literature data on the substrate specificity of the acceptor and donor sites. According to the proposed scheme only the elements of the donor and acceptor having a stable structure bind with the ribosome. The pre...

journal_title：Nucleic acids research

authors： Krayevsky AA,Kukhanova MK,Gottikh BP

更新日期：1975-12-01 00:00:00

abstract：:Zar1 was one of the earliest mammalian maternal-effect genes to be identified. Embryos derived from Zar1-null female mice are blocked before zygotic genome activation; however, the underlying mechanism remains unclear. By knocking out Zar1 and its homolog Zar2 in mice, we revealed a novel function of these genes in oo...

journal_title：Nucleic acids research

authors： Rong Y,Ji SY,Zhu YZ,Wu YW,Shen L,Fan HY

更新日期：2019-12-02 00:00:00

abstract：:Primer extension studies have shown that the Y-family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus P2 can preferentially insert C opposite N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (AAF-dG) [F. Boudsocq, S. Iwai, F. Hanaoka and R. Woodgate (2001) Nucleic Acids Res., 29, 4607-4616]. Our goal is to elucidate...

journal_title：Nucleic acids research

authors： Wang L,Broyde S

更新日期：2006-02-01 00:00:00

abstract：:Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are lar...

journal_title：Nucleic acids research

authors： Stulz J,Ackermann T,Appel B,Erdmann VA

更新日期：1981-08-11 00:00:00

abstract：:An electrophoretic system was established that resolves poly(adenosine diphosphate ribose), enzymatically synthesized polymer from NAD+, by size difference of one residue on polyacrylamide gel. The existence of a polymer of at least 65 residues was demonstrated by band counting in this system. The polymer showed a het...

journal_title：Nucleic acids research

authors： Tanaka M,Hayashi K,Sakura H,Miwa M,Matsushima T,Sugimura T

更新日期：1978-09-01 00:00:00

abstract：:With the improved rapid sequencing techniques, the earlier sequence of U2 RNA of Novikoff hepatoma (Shibata et al, J. Biol. Chem. 250, 3909-3920, 1975) was reanalyzed and modified. The improved sequence of U2 RNA is 188 (or 189) nucleotides long and is in register with a characterized U2 RNA pseudogene (Denison et al,...

journal_title：Nucleic acids research

authors： Reddy R,Henning D,Epstein P,Busch H

更新日期：1981-11-11 00:00:00

abstract：:Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process. We demonstrate its ...

journal_title：Nucleic acids research

authors： Kohli M,Rago C,Lengauer C,Kinzler KW,Vogelstein B

更新日期：2004-01-02 00:00:00

abstract：:Phospho3D is a database of three-dimensional (3D) structures of phosphorylation sites (P-sites) derived from the Phospho.ELM database, which also collects information on the residues surrounding the P-site in space (3D zones). The database also provides the results of a large-scale structural comparison of the 3D zone...

journal_title：Nucleic acids research

authors： Zanzoni A,Carbajo D,Diella F,Gherardini PF,Tramontano A,Helmer-Citterich M,Via A

更新日期：2011-01-01 00:00:00

abstract：:The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...

journal_title：Nucleic acids research

authors： Shaul YD,Yuan B,Thiru P,Nutter-Upham A,McCallum S,Lanzkron C,Bell GW,Sabatini DM

更新日期：2016-01-04 00:00:00

abstract：:In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3'- and 5'-ends of 261 yeast genes by run-on. The results obtained indicate that the 3'/5' run-on ratio varies among the genes studied by over 12 log(2) units. This ratio seem...

journal_title：Nucleic acids research

authors： Rodríguez-Gil A,García-Martínez J,Pelechano V,Muñoz-Centeno Mde L,Geli V,Pérez-Ortín JE,Chávez S

更新日期：2010-08-01 00:00:00

abstract：:Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-lin...

journal_title：Nucleic acids research

authors： Zwieb C,Brimacombe R

更新日期：1980-06-11 00:00:00

abstract：:The major immediate early regulatory region of human cytomegalovirus (HCMV) has a complex set of DNA sites through which both cellular and viral factors coordinately regulate immediate early gene expression. In undifferentiated human teratocarcinoma (T2) cells we have previously shown that major immediate early gene e...

journal_title：Nucleic acids research

authors： Kothari S,Baillie J,Sissons JG,Sinclair JH

更新日期：1991-04-25 00:00:00

abstract：:Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...

journal_title：Nucleic acids research

authors： Zamdborg L,Ma P

更新日期：2009-09-01 00:00:00

abstract：:In addition to maintaining the GenBank® nucleic acid sequence database, the National Center for Biotechnology Information (NCBI) provides analysis and retrieval resources for the data in GenBank and other biological data made available through the NCBI Web site. NCBI resources include Entrez, the Entrez Programming Ut...

journal_title：Nucleic acids research

authors： Sayers EW,Barrett T,Benson DA,Bolton E,Bryant SH,Canese K,Chetvernin V,Church DM,DiCuccio M,Federhen S,Feolo M,Fingerman IM,Geer LY,Helmberg W,Kapustin Y,Landsman D,Lipman DJ,Lu Z,Madden TL,Madej T,Maglott DR,Ma

更新日期：2011-01-01 00:00:00

abstract：:A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNAf binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low...

journal_title：Nucleic acids research

authors： Bagchi MK,Banerjee AC,Roy R,Chakrabarty I,Gupta NK

更新日期：1982-10-25 00:00:00

abstract：:Peptidome similarity analysis enables researchers to gain insights into differential peptide profiles, providing a robust tool to discriminate strain-specific peptides, true intra-species differences among biological replicates or even microorganism-phenotype variations. However, no in silico peptide fingerprinting so...

journal_title：Nucleic acids research

authors： Blanco-Míguez A,Fdez-Riverola F,Lourenço A,Sánchez B

更新日期：2017-07-03 00:00:00

abstract：:Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...

journal_title：Nucleic acids research

authors： Voltz K,Léonard J,Touceda PT,Conyard J,Chaker Z,Dejaegere A,Godet J,Mély Y,Haacke S,Stote RH

更新日期：2016-04-20 00:00:00

abstract：:The frequencies of each of the 257 468 complete protein coding sequences (CDSs) have been compiled from the taxonomical divisions of the GenBank DNA sequence database. The sum of the codons used by 8792 organisms has also been calculated. The data files can be obtained from the anonymous ftp sites of DDBJ, Kazusa and ...

journal_title：Nucleic acids research

authors： Nakamura Y,Gojobori T,Ikemura T

更新日期：2000-01-01 00:00:00

abstract：:ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-locus analyses of inb...

journal_title：Nucleic acids research

authors： Riso V,Cammisa M,Kukreja H,Anvar Z,Verde G,Sparago A,Acurzio B,Lad S,Lonardo E,Sankar A,Helin K,Feil R,Fico A,Angelini C,Grimaldi G,Riccio A

更新日期：2016-09-30 00:00:00

abstract：:Specific guanine-rich regions in human genome can form higher-order DNA structures called G-quadruplexes, which regulate many relevant biological processes. For instance, the formation of G-quadruplex at telomeres can alter cellular functions, inducing apoptosis. Thus, developing small molecules that are able to bind ...

journal_title：Nucleic acids research

authors： Di Leva FS,Novellino E,Cavalli A,Parrinello M,Limongelli V

更新日期：2014-05-01 00:00:00

abstract：:Key components of the translational apparatus, i.e. ribosomes, elongation factor EF-Tu and most aminoacyl-tRNA synthetases, are stereoselective and prevent incorporation of d-amino acids (d-aa) into polypeptides. The rare appearance of d-aa in natural polypeptides arises from post-translational modifications or non-ri...

journal_title：Nucleic acids research

authors： Achenbach J,Jahnz M,Bethge L,Paal K,Jung M,Schuster M,Albrecht R,Jarosch F,Nierhaus KH,Klussmann S

更新日期：2015-07-13 00:00:00