ZAR1 and ZAR2 are required for oocyte meiotic maturation by regulating the maternal transcriptome and mRNA translational activation.

abstract：:We examined the 3'-flanking regions required for accurate 3'-end formation of wheat histone H3 mRNA using gene expression in transformed sunflower cells. The introduction of mutations into the conserved sequence AATGGAAATG in the 3'-flanking region of plant histone genes, located 22 bp upstream from the polyadenylatio...

journal_title：Nucleic acids research

authors： Ohtsubo N,Iwabuchi M

更新日期：1994-03-25 00:00:00

abstract：:A microchip method has been developed for massive and parallel thermodynamic analyses of DNA duplexes. Fluorescently labeled oligonucleotides were hybridized with oligonucleotides immobilized in the 100 x 100 x 20 mum gel pads of the microchips. The equilibrium melting curves for all microchip duplexes were measured i...

journal_title：Nucleic acids research

authors： Fotin AV,Drobyshev AL,Proudnikov DY,Perov AN,Mirzabekov AD

更新日期：1998-03-15 00:00:00

abstract：:The study of bacterial isolates or communities requires the analysis of the therein included plasmids in order to provide an extensive characterization of the organisms. Plasmids harboring resistance and virulence factors are of especial interest as they contribute to the dissemination of antibiotic resistance. As the...

journal_title：Nucleic acids research

authors： Galata V,Fehlmann T,Backes C,Keller A

更新日期：2019-01-08 00:00:00

abstract：:In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...

journal_title：Nucleic acids research

authors： Faust M,Hastings KE,Millward S

更新日期：1975-08-01 00:00:00

abstract：:MicroRNAs (miRNAs) are short, non-protein coding RNAs that direct the widespread phenomenon of post-transcriptional regulation of metazoan genes. The mature approximately 22-nt long RNA molecules are processed from genome-encoded stem-loop structured precursor genes. Hundreds of such genes have been experimentally val...

journal_title：Nucleic acids research

authors： Gerlach D,Kriventseva EV,Rahman N,Vejnar CE,Zdobnov EM

更新日期：2009-01-01 00:00:00

abstract：:The cartilage matrix is composed of characteristic components including type II collagen, aggrecan and link protein. In this paper, we report two DNA elements that regulate the link protein gene. Using transient transfection assays with link protein gene constructs in chondrocytes, chloramphenicol acetyl transferase (...

journal_title：Nucleic acids research

authors： Rhodes C,Yamada Y

更新日期：1995-06-25 00:00:00

abstract：:The major mRNA degradation pathway involves deadenylation of the target molecule followed by decapping and, finally, 5'-->3' exonuclease digestion of the mRNA body. While yeast factors involved in the decapping and exonuclease degradation steps have been identified, the nature of the factor(s) involved in the deadenyl...

journal_title：Nucleic acids research

authors： Daugeron MC,Mauxion F,Séraphin B

更新日期：2001-06-15 00:00:00

abstract：:In plants, small interfering RNAs (siRNAs) can trigger a silencing signal that may spread within a tissue to adjacent cells or even systemically to other organs. Movement of the signal is initially limited to a few cells, but in some cases the signal can be amplified and travel over larger distances. How far silencing...

journal_title：Nucleic acids research

authors： de Felippes FF,Ott F,Weigel D

更新日期：2011-04-01 00:00:00

abstract：:Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNA-like (C3'-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself. We performed molecular dynamics simulations on five isosequential duplexes (LNA-DNA, LNA-LNA, LNA-R...

journal_title：Nucleic acids research

authors： Pande V,Nilsson L

更新日期：2008-03-01 00:00:00

abstract：:Accurate mapping of spliced RNA-Seq reads to genomic DNA has been known as a challenging problem. Despite significant efforts invested in developing efficient algorithms, with the human genome as a primary focus, the best solution is still not known. A recently introduced tool, TrueSight, has demonstrated better perfo...

journal_title：Nucleic acids research

authors： Burns PD,Li Y,Ma J,Borodovsky M

更新日期：2014-02-01 00:00:00

abstract：:Previous studies have shown that the yeast Candida albicans encodes a unique seryl-tRNA(CAG) that should decode the leucine codon CUG as serine. However, in vitro translation of several different CUG-containing mRNAs in the presence of this unusual seryl-tRNA(CAG) result in an apparent increase in the molecular weight...

journal_title：Nucleic acids research

authors： Santos MA,Tuite MF

更新日期：1995-05-11 00:00:00

abstract：:A suppressor tRNA(Tyr) and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-L-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNA(Tyr) gene was mutated, at three positions in the D arm, to generate the internal promoter for expression. However, this tRN...

journal_title：Nucleic acids research

authors： Sakamoto K,Hayashi A,Sakamoto A,Kiga D,Nakayama H,Soma A,Kobayashi T,Kitabatake M,Takio K,Saito K,Shirouzu M,Hirao I,Yokoyama S

更新日期：2002-11-01 00:00:00

abstract：:A better understanding of transcriptional and post-transcriptional regulation of gene expression in bacteria relies on studying their transcriptome. RNA sequencing methods are used not only to assess RNA abundance but also the exact boundaries of primary and processed transcripts. Here, we developed a method, called i...

journal_title：Nucleic acids research

authors： Le Rhun A,Lécrivain AL,Reimegård J,Proux-Wéra E,Broglia L,Della Beffa C,Charpentier E

更新日期：2017-03-17 00:00:00

abstract：:Genome organization into transcriptionally active domains denotes one of the first levels of gene expression regulation. Although the chromatin domain concept is generally accepted, only little is known on how domain organization impacts the regulation of differential gene expression. Insulators might hold answers to ...

journal_title：Nucleic acids research

authors： Furlan-Magaril M,Rebollar E,Guerrero G,Fernández A,Moltó E,González-Buendía E,Cantero M,Montoliu L,Recillas-Targa F

更新日期：2011-01-01 00:00:00

abstract：:The small subunit ribosomal RNA gene (16S rRNA) has been successfully used to catalogue and study the diversity of prokaryotic species and communities but it offers limited resolution at the species and finer levels, and cannot represent the whole-genome diversity and fluidity. To overcome these limitations, we introd...

journal_title：Nucleic acids research

authors： Rodriguez-R LM,Gunturu S,Harvey WT,Rosselló-Mora R,Tiedje JM,Cole JR,Konstantinidis KT

更新日期：2018-07-02 00:00:00

abstract：:The PR264/SC35 splicing factor belongs to the family of SR proteins which function as essential and alternative splicing factors. Here, we report that the human PR264/SC35 locus is bidirectionally transcribed. Double in situ hybridization experiments have allowed simultaneous detection of sense and antisense RNA in hu...

journal_title：Nucleic acids research

authors： Sureau A,Soret J,Guyon C,Gaillard C,Dumon S,Keller M,Crisanti P,Perbal B

更新日期：1997-11-15 00:00:00

abstract：:The beta protein encoded by the Streptococcus pyogenes plasmid pSM19035 is a site-specific recombinase involved in both resolution of plasmid multimers into monomers and DNA inversion. It has been proposed that the DNA region to which the beta recombinase binds to mediate recombination includes a promoter from which o...

journal_title：Nucleic acids research

authors： Rojo F,Alonso JC

更新日期：1994-05-25 00:00:00

abstract：:CTCF plays a pivotal role in mediating chromatin interactions, but it does not do so alone. A number of factors have been reported to co-localize with CTCF and regulate CTCF loops, but no comprehensive analysis of binding partners has been performed. This prompted us to identify CTCF loop participants and regulators b...

journal_title：Nucleic acids research

authors： Hu G,Dong X,Gong S,Song Y,Hutchins AP,Yao H

更新日期：2020-09-25 00:00:00

abstract：:We have previously reported that transcription from a distal promoter (promoter B) of the estrogen receptor alpha (ERalpha) gene is responsible for the increased expression of ERalpha in human breast carcinomas. This paper first characterized the promoter B region in terms of transient transfection experiments with lu...

journal_title：Nucleic acids research

authors： Tanimoto K,Eguchi H,Yoshida T,Hajiro-Nakanishi K,Hayashi S

更新日期：1999-02-01 00:00:00

abstract：:Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...

journal_title：Nucleic acids research

authors： Sparrow DB,Latinkic B,Mohun TJ

更新日期：2000-02-15 00:00:00

abstract：:The apolipoprotein AI (apoAI) promoter/enhancer contains multiple cis -acting elements on which a variety of hepatocyte-enriched and ubiquitous transcription factors function synergistically to regulate liver-specific transcription. Adenovirus E1A proteins repress tissue-specific gene expression and disrupt the differ...

journal_title：Nucleic acids research

authors： Kilbourne EJ,Evans MJ,Karathanasis SK

更新日期：1998-04-01 00:00:00

abstract：:4'-Acylated thymidines represent a new class of DNA chain terminators, since they have been shown to act as post-incorporation chain-terminating nucleotides despite the presence of a free 3'-hydroxyl group. Here, we describe the action of the 4'-acetyl- (MeTTP) and 4'-propanoylthymidine 5'-triphosphate (EtTTP) on HIV-...

journal_title：Nucleic acids research

authors： Marx A,Amacker M,Stucki M,Hübscher U,Bickle TA,Giese B

更新日期：1998-09-01 00:00:00

abstract：:Expression of the wild-type p53 tumor suppressor gene has been found to play an important role in the regulation of cellular proliferation and differentiation. In addition, in many transformed cells and primary tumors, the gene has undergone allelic deletions and mutant forms of the p53 gene are expressed at elevated ...

journal_title：Nucleic acids research

authors： Reisman D,Rotter V

更新日期：1993-01-25 00:00:00

abstract：:BIOSMILE web search (BWS), a web-based NCBI-PubMed search application, which can analyze articles for selected biomedical verbs and give users relational information, such as subject, object, location, manner, time, etc. After receiving keyword query input, BWS retrieves matching PubMed abstracts and lists them along ...

journal_title：Nucleic acids research

authors： Dai HJ,Huang CH,Lin RT,Tsai RT,Hsu WL

更新日期：2008-07-01 00:00:00

abstract：:RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions (UTRs). Yet, the underlying mechanisms and coding requirements for efficient co-transcriptional folding remain unclear. Traditional a...

journal_title：Nucleic acids research

authors： Xayaphoummine A,Viasnoff V,Harlepp S,Isambert H

更新日期：2007-01-01 00:00:00

abstract：:Extensive research has characterized distinct exogenous RNAi pathways interfering in gene expression during vegetative growth of the unicellular model ciliate Paramecium. However, role of RNAi in endogenous transcriptome regulation, and environmental adaptation is unknown. Here, we describe the first genome-wide profi...

journal_title：Nucleic acids research

authors： Karunanithi S,Oruganti V,Marker S,Rodriguez-Viana AM,Drews F,Pirritano M,Nordström K,Simon M,Schulz MH

更新日期：2019-09-05 00:00:00

abstract：:A vexing difficulty of the RNA world hypothesis is how RNA molecules of significant complexity could ever have evolved given their susceptibility to degradation. One way degradation might have been reduced is through low temperature. Here we report that truncated and fragmented derivatives of the hairpin ribozyme can ...

journal_title：Nucleic acids research

authors： Vlassov AV,Johnston BH,Landweber LF,Kazakov SA

更新日期：2004-05-25 00:00:00

abstract：:Each of the major U snRNP polypeptides from human cells was purified by electroelution from SDS-polyacrylamide gels. Rabbit antisera could be obtained against the individual proteins 70K, A, B', B and D, although rabbits failed to elicit antibodies against E, F and G. A strong structural homology was found between pro...

journal_title：Nucleic acids research

authors： Reuter R,Rothe S,Lührmann R

更新日期：1987-05-26 00:00:00

abstract：:Inhibition of an RNA processing reaction after treatment with the Ca2(+)-dependent micrococcal nuclease (MN) is often used as a criterion for the presence of a required RNA or ribonucleoprotein component in the system. Following MN digestion, the nuclease is inactivated with EGTA and radiolabeled substrate is added to...

journal_title：Nucleic acids research

authors： Wang MJ,Gegenheimer P

更新日期：1990-11-25 00:00:00

abstract：:The chloroplast ribosomal protein CS1 is an essential component of the plastids translational machinery involved in translation initiation. Southern analysis suggests that the corresponding nuclear gene is present in one copy in the spinach genome. We have isolated and sequenced the gene (rps1) to study its expression...

journal_title：Nucleic acids research

authors： Franzetti B,Zhou DX,Mache R

更新日期：1992-08-25 00:00:00