Protein synthesis in rabbit reticulocytes: characteristics of CO-eIF-2 protein complex.

Abstract:

:A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNAf binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low eIF-2 levels. (2) Stimulation is partially resistant to heat and NEM treatment, and thus appears to be due to the combined action of both heat and NEM-insensitive Co-eIF-2A, and heat and NEM-sensitive Co-eIF-2C activities. (3) [3H]GDP bound in eIF-2 . [3H]GDP complex is rapidly displaced by unlabelled GTP during ternary complex formation Co-eIF-2 stimulates Met-tRNAf binding to eIF-2 even when added after the [3H]GDP from eIF-2 . [3H]GDP has been completely displaced. This indicates that Co-eIF-2-stimulation is not due to GDP displacement from eIF-2 . GDP. We propose that eIF-2 molecules become inactive in the presence of Mg2+ and at high dilution, and Co-eIF-2 restores the inactive eIF-2 molecules into an active form.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Bagchi MK,Banerjee AC,Roy R,Chakrabarty I,Gupta NK

doi

10.1093/nar/10.20.6501

subject

Has Abstract

pub_date

1982-10-25 00:00:00

pages

6501-10

issue

20

eissn

0305-1048

issn

1362-4962

journal_volume

10

pub_type

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