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Regulation of the small regulatory RNA MicA by ribonuclease III: a target-dependent pathway.

Abstract：

:MicA is a trans-encoded small non-coding RNA, which downregulates porin-expression in stationary-phase. In this work, we focus on the role of endoribonucleases III and E on Salmonella typhimurium sRNA MicA regulation. RNase III is shown to regulate MicA in a target-coupled way, while RNase E is responsible for the control of free MicA levels in the cell. We purified both Salmonella enzymes and demonstrated that in vitro RNase III is only active over MicA when in complex with its targets (whether ompA or lamB mRNAs). In vivo, MicA is demonstrated to be cleaved by RNase III in a coupled way with ompA mRNA. On the other hand, RNase E is able to cleave unpaired MicA and does not show a marked dependence on its 5' phosphorylation state. The main conclusion of this work is the existence of two independent pathways for MicA turnover. Each pathway involves a distinct endoribonuclease, having a different role in the context of the fine-tuned regulation of porin levels. Cleavage of MicA by RNase III in a target-dependent fashion, with the concomitant decay of the mRNA target, strongly resembles the eukaryotic RNAi system, where RNase III-like enzymes play a pivotal role.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Viegas SC,Silva IJ,Saramago M,Domingues S,Arraiano CM

doi

10.1093/nar/gkq1239

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

2918-30

issue

7

eissn

0305-1048

issn

1362-4962

pii

gkq1239

journal_volume

39

pub_type

杂志文章

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