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The NF-kappa B transcription factor induces DNA bending which is modulated by its 65-kD subunit.

Abstract：

:This study describes a novel functional property of the eukaryotic transcription factor NF-kappa B in altering the DNA structure. We observed that the binding of purified NF-kappa B to DNA was facilitated by spermine and cations which are known to promote DNA bending, and by a nick in one position at the 3'-end of the binding site. Furthermore, the position of the NF-kappa B binding sequence 5'-GGGACTTTCC-3' (kappa B motif) within circularly permutated DNA fragments had a profound influence on the mobility of NF-kappa B-DNA complexes in a gel retardation assay while the mobility of unbound DNA fragments did not depend on the position of the kappa B motif. The mobility effect was slightly reduced at increased temperature. These observations suggested that binding of NF-kappa B to DNA induces bending. The estimated bending angle induced by the 50-kD DNA binding subunit of NF-kappa B (p50) was smaller than that induced by p50 which was associated with the 65-kD non-DNA binding subunit (p65). Moreover, the presence of p65 appeared to result in a shift of the bending center from the middle towards the 3'-end of the kappa B motif. This shows a role for the p65 subunit of NF-kappa B in modulating the extent and altering the position of protein-induced DNA bending.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Schreck R,Zorbas H,Winnacker EL,Baeuerle PA

doi

10.1093/nar/18.22.6497

subject

Has Abstract

pub_date

1990-11-25 00:00:00

pages

6497-502

issue

22

eissn

0305-1048

issn

1362-4962

journal_volume

18

pub_type

杂志文章

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