Abstract：
:Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog species Rana pipiens. The isolated clones were approximately 90% identical to a predicted transposase gene sequence from Xenopus laevis, but contained an unpredicted, approximately 180 bp region encoding the N-terminus of the putative transposase. None of these native genes was found to be active. Therefore, a consensus sequence of the transposase gene was derived. This engineered transposase and the transposon inverted repeats together constitute the components of a novel transposon system that we named Frog Prince (FP). FP has only approximately 50% sequence similarity to Sleeping Beauty (SB), and catalyzes efficient cut-and-paste transposition in fish, amphibian and mammalian cell lines. We demonstrate high-efficiency gene trapping in human cells using FP transposition. FP is the most efficient DNA-based transposon from vertebrates described to date, and shows approximately 70% higher activity in zebrafish cells than SB. Frog Prince can greatly extend our possibilities for genetic analyses in vertebrates.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Miskey C,Izsvák Z,Plasterk RH,Ivics Zdoi
10.1093/nar/gkg910keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
6873-81issue
23eissn
0305-1048issn
1362-4962journal_volume
31pub_type
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：2006-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1996-05-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
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更新日期：2005-10-26 00:00:00
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journal_title：Nucleic acids research
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更新日期：2007-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2016-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-01-04 00:00:00
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pub_type： 杂志文章
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