Poly(pyrimidine) . poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH.

abstract：:A 9.2 kb segment of the maxi-circle of Trypanosoma brucei mitochondrial DNA contains the genes for cytochrome c oxidase subunits I and II (coxI and coxII) and seven Unassigned Reading Frames ("URFs"). The genes for coxI and coxII display considerable homology at the aminoacid level (38 and 25%, respectively) to the co...

journal_title：Nucleic acids research

authors： Hensgens LA,Brakenhoff J,De Vries BF,Sloof P,Tromp MC,Van Boom JH,Benne R

更新日期：1984-10-11 00:00:00

abstract：:PUF proteins, named for Drosophila Pumilio (PUM) and Caenorhabditis elegans fem-3-binding factor (FBF), recognize specific sequences in the mRNAs they bind and control. RNA binding by classical PUF proteins is mediated by a characteristic PUM homology domain (PUM-HD). The Puf1 and Puf2 proteins possess a distinct arch...

journal_title：Nucleic acids research

authors： Qiu C,Dutcher RC,Porter DF,Arava Y,Wickens M,Hall TMT

更新日期：2019-09-19 00:00:00

abstract：:Three Rat1/Xrn2 homologues exist in Arabidopsis thaliana: nuclear AtXRN2 and AtXRN3, and cytoplasmic AtXRN4. The latter has a role in degrading 3' products of miRNA-mediated mRNA cleavage, whereas all three proteins act as endogenous post-transcriptional gene silencing suppressors. Here we show that, similar to yeast ...

journal_title：Nucleic acids research

authors： Zakrzewska-Placzek M,Souret FF,Sobczyk GJ,Green PJ,Kufel J

更新日期：2010-07-01 00:00:00

abstract：:Mitochondrial aprataxin (APTX) protects the mitochondrial genome from the consequence of ligase failure by removing the abortive ligation product, i.e. the 5'-adenylate (5'-AMP) group, during DNA replication and repair. In the absence of APTX activity, blocked base excision repair (BER) intermediates containing the 5'...

journal_title：Nucleic acids research

authors： Çaglayan M,Prasad R,Krasich R,Longley MJ,Kadoda K,Tsuda M,Sasanuma H,Takeda S,Tano K,Copeland WC,Wilson SH

更新日期：2017-09-29 00:00:00

abstract：:Gene trap mutagenesis of mouse embryonic stem cells generates random loss-of-function mutations, which can be identified by a sequence tag and can often report the endogenous expression of the mutated gene. The Centre for Modeling Human Disease is performing expression- and sequence-based screens of gene trap insertio...

journal_title：Nucleic acids research

authors： To C,Epp T,Reid T,Lan Q,Yu M,Li CY,Ohishi M,Hant P,Tsao N,Casallo G,Rossant J,Osborne LR,Stanford WL

更新日期：2004-01-01 00:00:00

abstract：:Sequences from -3500 to +1365 of the chicken histone H5 gene have been analyzed for the presence of cis-acting elements in H5 expressing (transformed CFU-E) and non-expressing cells (fibroblasts). The region from -3500 to -115 had little effect on transcription. Proximal upstream sequences contain a negative element (...

journal_title：Nucleic acids research

authors： Rousseau S,Renaud J,Ruiz-Carrillo A

更新日期：1989-09-25 00:00:00

abstract：:The Bayesian regularization method for high-throughput differential analysis, described in Baldi and Long (A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes. Bioinformatics 2001: 17: 509-519) and implemented in the Cyber-T web server, is ...

journal_title：Nucleic acids research

authors： Kayala MA,Baldi P

更新日期：2012-07-01 00:00:00

abstract：:This review takes a second look at a set of mRNAs that purportedly employ an alternative mechanism of initiation when cap-dependent translation is reduced during mitosis or stress conditions. A closer look is necessary because evidence cited in support of the internal initiation hypothesis is often flawed. When putati...

journal_title：Nucleic acids research

authors： Kozak M

更新日期：2005-11-28 00:00:00

abstract：:Nucleotide excision DNA repair is mechanistically conserved across all kingdoms of life. In prokaryotes, this multi-enzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase. To examine how UvrC locates the UvrB-DNA pre-incision complex at a site of damage, we have labeled UvrB and UvrC with differ...

journal_title：Nucleic acids research

authors： Hughes CD,Wang H,Ghodke H,Simons M,Towheed A,Peng Y,Van Houten B,Kad NM

更新日期：2013-05-01 00:00:00

abstract：:Genome-wide transcriptome profiling has enabled non-supervised classification of tumours, revealing different sub-groups characterized by specific gene expression features. However, the biological significance of these subtypes remains for the most part unclear. We describe herein an interactive platform, Minimum Span...

journal_title：Nucleic acids research

authors： Lemieux S,Sargeant T,Laperrière D,Ismail H,Boucher G,Rozendaal M,Lavallée VP,Ashton-Beaucage D,Wilhelm B,Hébert J,Hilton DJ,Mader S,Sauvageau G

更新日期：2017-07-27 00:00:00

abstract：:The genome of Kirsten murine sarcoma virus was formed by recombination between Kirsten murine leukaemia virus sequences, and rat sequences derived from a retrovirus-like '30S' (VL30) genetic element encompassing the Kras oncogene. Using cloned DNAs we have determined the nucleotide sequences of the long terminal repea...

journal_title：Nucleic acids research

authors： Norton JD,Connor J,Avery RJ

更新日期：1984-09-11 00:00:00

abstract：:Spliceosomes are assembled in stages. The first stage forms complex E, which is characterized by the presence of U1 snRNPs base-paired to the 5' splice site, components recognizing the 3' splice site and proteins thought to connect them. The splice sites are held in close proximity and the pre-mRNA is committed to spl...

journal_title：Nucleic acids research

authors： Hodson MJ,Hudson AJ,Cherny D,Eperon IC

更新日期：2012-08-01 00:00:00

abstract：:DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than th...

journal_title：Nucleic acids research

authors： Rill RL,Nelson DA,Oosterhof DK,Hozier JC

更新日期：1977-04-01 00:00:00

abstract：:We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing ...

journal_title：Nucleic acids research

authors： Pfoh R,Laatsch H,Sheldrick GM

更新日期：2008-06-01 00:00:00

abstract：:The nucleotide sequence for human argininosuccinate synthetase cDNA was determined by analysis of six clones isolated from a single experiment. The sequence covered 1623 nucleotides including 76 bases of poly(A) and contained a 1236 nucleotide open reading frame encoding a protein of 46,434 daltons. In one cDNA isolat...

journal_title：Nucleic acids research

authors： Bock HG,Su TS,O'Brien WE,Beaudet AL

更新日期：1983-09-24 00:00:00

abstract：:In mammalian cells, DNA damage induces robust changes in gene expression and these changes contribute to the proper execution of cellular responses to DNA damage, including DNA repair, cell cycle arrest and apoptosis. The transcription factor E2F-1 has been suggested to play a key role in the regulation of cell cycle-...

journal_title：Nucleic acids research

authors： Höfferer M,Wirbelauer C,Humar B,Krek W

更新日期：1999-01-15 00:00:00

abstract：:The thymine analog 5-chlorouridine, first reported in the 1950s as anti-tumor agent, is known as an effective mutagen, clastogen and toxicant as well as an effective inducer of sister-chromatid exchange. Recently, the first microorganism with a chemically different genome was reported; the selected Escherichia coli st...

journal_title：Nucleic acids research

authors： Patra A,Harp J,Pallan PS,Zhao L,Abramov M,Herdewijn P,Egli M

更新日期：2013-02-01 00:00:00

abstract：:Histone modifications are fundamental to chromatin structure and transcriptional regulation, and are recognized by a limited number of protein folds. Among these folds are PHD fingers, which are present in most chromatin modification complexes. To date, about 15 PHD finger domains have been structurally characterized,...

journal_title：Nucleic acids research

authors： Slama P,Geman D

更新日期：2011-03-01 00:00:00

abstract：:In recent years, the increase in the amounts of available genomic data has made it easier to appreciate the extent by which organisms increase their genetic diversity through horizontally transferred genetic material. Such transfers have the potential to give rise to extremely dynamic genomes where a significant propo...

journal_title：Nucleic acids research

authors： Tsirigos A,Rigoutsos I

更新日期：2005-02-16 00:00:00

abstract：:Treatment of cells with DNA damaging agents leads to induction of a variety of genes involved in different cellular processes. We have applied a lacZ-based gene trap strategy to search for new mammalian genes induced by genotoxic stress. A population of 32 x 10(3) neo r clones stably transfected with a gene trap vecto...

journal_title：Nucleic acids research

authors： Menichini P,Viaggi S,Gallerani E,Fronza G,Ottaggio L,Comes A,Ellwart JW,Abbondandolo A

更新日期：1997-12-01 00:00:00

abstract：:Assessment of the fidelity of gene expression is crucial to understand cell homeostasis. Here we present a highly sensitive method for the systematic Quantification of Rare Amino acid Substitutions (QRAS) using absolute quantification by targeted mass spectrometry after chromatographic enrichment of peptides with miss...

journal_title：Nucleic acids research

authors： Garofalo R,Wohlgemuth I,Pearson M,Lenz C,Urlaub H,Rodnina MV

更新日期：2019-04-08 00:00:00

abstract：:Despite much information as to the structure and function of the general transcription factors, little is known about the regulation of their expression. Transcription of the Saccharomyces cerevisiae SUA7 (TFIIB) gene results in the formation of two discrete transcripts. It was originally reported that the two transcr...

journal_title：Nucleic acids research

authors： Hoopes BC,Bowers GD,DiVisconte MJ

更新日期：2000-11-15 00:00:00

abstract：:The 80S ribosome from Saccharomyces cerevisiae has been reconstructed from cryo electron micrographs to a resolution of 35 A. It is strikingly similar to the 70S ribosome from Escherichia coli, while displaying the characteristic eukaryotic features familiar from reconstructions of ribosomes from higher eukaryotes. As...

journal_title：Nucleic acids research

authors： Verschoor A,Warner JR,Srivastava S,Grassucci RA,Frank J

更新日期：1998-01-15 00:00:00

abstract：:In this study, we demonstrate that the lack of retinoic acid-related orphan receptor (ROR) γ or α expression in mice significantly reduced the peak expression level of Cry1, Bmal1, E4bp4, Rev-Erbα and Per2 in an ROR isotype- and tissue-selective manner without affecting the phase of their rhythmic expression. Analysis...

journal_title：Nucleic acids research

authors： Takeda Y,Jothi R,Birault V,Jetten AM

更新日期：2012-09-01 00:00:00

abstract：:The channel proteins belonging to the major intrinsic proteins (MIP) superfamily are diverse and are found in all forms of life. Water-transporting aquaporin and glycerol-specific aquaglyceroporin are the prototype members of the MIP superfamily. MIPs have also been shown to transport other neutral molecules and gases...

journal_title：Nucleic acids research

authors： Gupta AB,Verma RK,Agarwal V,Vajpai M,Bansal V,Sankararamakrishnan R

更新日期：2012-01-01 00:00:00

abstract：:The primary structures of two overlapping novel alpha-tubulin cDNA clones isolated from a Macaca fascicularis testis cDNA library and the corresponding human gene are presented. Although the general structure of the human gene conforms to that of previously described mammalian alpha-tubulin genes, there is a surprisin...

journal_title：Nucleic acids research

authors： Dobner PR,Kislauskis E,Wentworth BM,Villa-Komaroff L

更新日期：1987-01-12 00:00:00

abstract：:An important problem in genomics is automatically clustering homologous proteins when only sequence information is available. Most methods for clustering proteins are local, and are based on simply thresholding a measure related to sequence distance. We first show how locality limits the performance of such methods by...

journal_title：Nucleic acids research

authors： Paccanaro A,Casbon JA,Saqi MA

更新日期：2006-03-17 00:00:00

abstract：:The mouse c-Ki-ras protooncogene promoter contains a homopurine-homopyrimidine domain that exhibits S1 nuclease sensitivity in vitro. We have studied the structure of this DNA region in a supercoiled state using a number of chemical probes for non-B DNA conformations including diethyl pyrocarbonate, osmium tetroxide, ...

journal_title：Nucleic acids research

authors： Pestov DG,Dayn A,Siyanova EYu,George DL,Mirkin SM

更新日期：1991-12-11 00:00:00

abstract：:Replacement of alpha-, beta- and gamma-phosphate groups in 2'-deoxynucleoside 5'-triphosphates (dNTP) with phosphonate groups yields a new set of dNTP mimics with potential biological and therapeutic applications. Here, we describe the synthesis of 15 new dNTPs modified at alpha-, beta- and gamma-phosphates containing...

journal_title：Nucleic acids research

authors： Alexandrova LA,Skoblov AY,Jasko MV,Victorova LS,Krayevsky AA

更新日期：1998-02-01 00:00:00

abstract：:Using a bio-oligo pull-down DNA-binding assay we investigated the binding capacity of endogenous, DNA damage-induced p53 in human diploid fibroblasts to several p53-responsive elements (REs) present in p53-regulated genes. During the course of p53 accumulation, we observed a decrease in p53 binding to the GADD45 but n...

journal_title：Nucleic acids research

authors： Buzek J,Latonen L,Kurki S,Peltonen K,Laiho M

更新日期：2002-06-01 00:00:00