Abstract:
:Sequences from -3500 to +1365 of the chicken histone H5 gene have been analyzed for the presence of cis-acting elements in H5 expressing (transformed CFU-E) and non-expressing cells (fibroblasts). The region from -3500 to -115 had little effect on transcription. Proximal upstream sequences contain a negative element (UNE, -115 to -95), capable to also repress the activity of the heterologous HSV tk promoter, and two positive elements, a consensus GC-box (-83 to -74) and a proximal element (UPE, -54 to -38). The sequence of the UPE is highly related to the histone H4 subtype-specific element and it has been conserved in the duck H5 and the human and mouse H1(0) genes at equivalent positions. Although the effect of the UNE, GC-box and UPE was not tissue-specific, sequences from -38 to +77 appear to confer a degree of tissue specificity to the promoter. An activating erythroid-specific element (DE) was found downstream of the H5 gene (+1042 to +1185). The activity of the DE was modest but independent of position and orientation and required the presence of the promoter proximal elements. The DE harbors the sequence AGATAA that is recognized by a protein factor, presumably the same that binds to other erythrocyte-specific enhancers. The low activity of DE in the CFU-E may be related to the low concentration of the AGATAA-binding factor in the differentiation-blocked cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rousseau S,Renaud J,Ruiz-Carrillo Adoi
10.1093/nar/17.18.7495subject
Has Abstractpub_date
1989-09-25 00:00:00pages
7495-511issue
18eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract::Bacteriophage T4 Dda helicase has recently been shown to be active as a monomer for unwinding of short duplex oligonucleotides and for displacing streptavidin from 3'-biotinylated oligonucleotides. However, its activity for streptavidin displacement and DNA unwinding has been shown to increase as the number of Dda mol...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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doi:10.1093/nar/15.11.4375
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.17.6883
更新日期:1987-09-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.21.8193
更新日期:1984-11-12 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1143
更新日期:2007-01-01 00:00:00
abstract::Riboswitch RNAs fold into complex tertiary structures upon binding to their cognate ligand. Ligand recognition is accomplished by key residues in the binding pocket. In addition, it often crucially depends on the stability of peripheral structural elements. The ligand-bound complex of the guanine-sensing riboswitch fr...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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doi:10.1093/nar/gky1078
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.3.921
更新日期:1987-02-11 00:00:00
abstract::The inducibility of the vir genes (virA, -B, -C, -D, -E, and -G) on pRiA4 was examined at the transcriptional level, and the RNA-starting sites were determined by S1-nuclease mapping and primer-extension experiments. All of these genes were inducible, while virA, -E, and -G were transcribed even under noninducing cond...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.21.8711
更新日期:1989-11-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm515
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl707
更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.7.2819
更新日期:1989-04-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:1979-01-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1999-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2019-09-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa162
更新日期:2020-05-07 00:00:00
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journal_title:Nucleic acids research
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更新日期:2002-10-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2017-05-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.9.1557
更新日期:1995-05-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2006-06-06 00:00:00