Abstract:
:Substantial amounts of DNA (at least 25 microgram per cm2) can be stably bound to diazobenzyloxymethyl (DBM)-paper. Complementary RNA will hybridize to the DNA paper almost completely in 24 hours. Using several different conditions of hybridization and washing, the background of RNA bound non-specifically is very low (between 0.01 and 0.02%) and the efficiency of hybridization is very high (75 to 50% of complementary RNA is bound and retained through the washing procedure). Because the DNA is bound to the paper convalently, it is retained through all the washing and elution steps, and the DNA papers can be re-used many times.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Stark GR,Williams JGdoi
10.1093/nar/6.1.195subject
Has Abstractpub_date
1979-01-01 00:00:00pages
195-203issue
1eissn
0305-1048issn
1362-4962journal_volume
6pub_type
杂志文章abstract::The Genomes Online Database (GOLD) (https://gold.jgi.doe.gov) is an open online resource, which maintains an up-to-date catalog of genome and metagenome projects in the context of a comprehensive list of associated metadata. Information in GOLD is organized into four levels: Study, Biosample/Organism, Sequencing Proje...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky977
更新日期:2019-01-08 00:00:00
abstract::The task of gene identification frequently confronting researchers working with both novel and well studied genomes can be conveniently and reliably solved with the help of the GeneMark web software (http://opal.biology.gatech.edu/GeneMark/). The website provides interfaces to the GeneMark family of programs designed ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki487
更新日期:2005-07-01 00:00:00
abstract::Alu elements have repeatedly been found involved in gene rearrangements in humans. Although these elements have been suggested to stimulate gene rearrangements, sparse information is available for the possible mechanism(s) of these events. Here we present a compilation of Alu elements that have been involved in recomb...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.2.256
更新日期:1995-01-25 00:00:00
abstract::S/MARt DB, the S/MAR transaction database, is a relational database covering scaffold/matrix attached regions (S/MARs) and nuclear matrix proteins that are involved in the chromosomal attachment to the nuclear scaffold. The data are mainly extracted from original publications, but a World Wide Web interface for direct...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.1.372
更新日期:2002-01-01 00:00:00
abstract::The model mRNA (MR), 11-mer RNA containing two nitroxide spin labels at the 5'- and 3'-terminal nucleotides and prone to form a stable homodimer (MR)2, was used for Electron Paramagnetic Resonance study of structural rearrangements in mRNA occurring upon its binding to human 80S ribosomes. The formation of two differe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1136
更新日期:2018-01-25 00:00:00
abstract::In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.16.3327
更新日期:2001-08-15 00:00:00
abstract::Eukaryotic cells possess several distinct mismatch repair pathways. A mismatch can be introduced in retroviral double-stranded DNA by a pre-existing mutation within the primer binding site (PBS) of the viral RNA genome. In order to evaluate mismatch repair of retroviral double-stranded DNA, Moloney leukemia virus (MLV...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.12.2302
更新日期:2000-06-15 00:00:00
abstract::Using an in vitro transcription system for Saccharomyces cerevisiae RNA polymerase I, we have analyzed Pol I promoter deletion mutants and mapped the boundaries of the promoter between positions -155 and +27. The 5'-boundary of the minimal core promoter capable of transcription initiation, however, was found to lie be...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.19.5363
更新日期:1991-10-11 00:00:00
abstract::Unfolding of the nucleosomes in transcriptionally active chromatin uncovers the sulfhydryl groups of histone H3 and permits the selective recovery of the unfolded nucleosomes by mercury-affinity chromatography. This new technique has been used to compare the nucleosomal proteins and their postsynthetic modifications i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.11.4375
更新日期:1987-06-11 00:00:00
abstract::Sequence-specific DNA-binding proteins play a key role in many fundamental biological processes, such as transcription, DNA replication and recombination. Very often, these DNA-binding proteins introduce structural changes to the target DNA-binding sites including DNA bending, twisting or untwisting and wrapping, whic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq078
更新日期:2010-06-01 00:00:00
abstract::The poly(A) signal and downstream elements with transcriptional pausing activity play an important role in termination of RNA polymerase II transcription. We show that an intronic sequence derived from the plant seed protein gene (AmA1) specifically acts as a transcriptional terminator in the fission yeast, Schizosacc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf334
更新日期:2002-07-01 00:00:00
abstract::Given that greater than 90% of the human genome is expressed, it is logical to assume that post-transcriptional regulatory mechanisms must be the primary means of controlling the flow of information from mRNA to protein. This report describes a robust approach that includes in silico, in vitro and in cellulo experimen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq557
更新日期:2010-11-01 00:00:00
abstract::A microchip method has been developed for massive and parallel thermodynamic analyses of DNA duplexes. Fluorescently labeled oligonucleotides were hybridized with oligonucleotides immobilized in the 100 x 100 x 20 mum gel pads of the microchips. The equilibrium melting curves for all microchip duplexes were measured i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.6.1515
更新日期:1998-03-15 00:00:00
abstract::This work presents the Apo-Holo DataBase (AH-DB, http://ahdb.ee.ncku.edu.tw/ and http://ahdb.csbb.ntu.edu.tw/), which provides corresponding pairs of protein structures before and after binding. Conformational transitions are commonly observed in various protein interactions that are involved in important biological f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr940
更新日期:2012-01-01 00:00:00
abstract::The MED1/TRAP220 subunit of the Mediator plays a key role in facilitating ligand-dependent interactions of this multisubunit coactivator complex with nuclear receptors through their ligand binding domains. The isolated MED1/TRAP220 protein previously was shown to interact with glucocorticoid receptor (GR) in a ligand-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm661
更新日期:2007-01-01 00:00:00
abstract::Diethyl pyrocarbonate reacts with the single-stranded loops of cruciform structures with great selectivity. Adenine bases are carbethoxylated, as a result of which the backbone may be cleaved with piperidine, and the level of chemical modification at each base may be determined. We have studied the ColE1 and (A-T)34 c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.10.3995
更新日期:1986-05-27 00:00:00
abstract::Alternative RNA splicing is an essential process to yield proteomic diversity in eukaryotic cells, and aberrant splicing is often associated with numerous human diseases and cancers. We recently described serine/arginine-rich splicing factor 3 (SRSF3 or SRp20) being a proto-oncogene. However, the SRSF3-regulated splic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1500
更新日期:2016-02-29 00:00:00
abstract::Alternative splicing and polyadenylation were observed pervasively in eukaryotic messenger RNAs. These alternative isoforms could either be consequences of physiological regulation or stochastic noise of RNA processing. To quantify the extent of stochastic noise in splicing and polyadenylation, we analyzed the alterna...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1271
更新日期:2013-02-01 00:00:00
abstract::The sequence of the 1173 nt 12S kinetoplast ribosomal RNA from Leishmania tarentolae was determined from the maxicircle DNA sequence, and the 5' and 3' ends localized by primer runoff and S1 nuclease protection experiments. The gene was shown to be free of introns by S1 nuclease analysis. A partial secondary structure...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.7.2337
更新日期:1985-04-11 00:00:00
abstract::DNA molecules with covalently sealed (hairpin) ends are probable intermediates in V(D)J recombination. According to current models hairpin ends are opened to produce short single-stranded extensions that are thought to be precursors of a particular type of extra nucleotides, termed P nucleotides, which are frequently ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.19.3872
更新日期:1995-10-11 00:00:00
abstract::Two genomic DNAs with a size of approximately 2.8 kb, isolated from the liver of Bungarus multicinctus (Taiwan banded krait), encode the precursors of the long neurotoxins, alpha-Bgt(A31) and alpha-Bgt(V31), respectively. Both genes share virtually identical overall organization with three exons separated by two intro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.20.3970
更新日期:1999-10-15 00:00:00
abstract::Exon arrays are regularly used to analyze differential splicing events. GeneChip Gene 1.0 ST Arrays (gene arrays) manufactured by Affymetrix, Inc. are primarily used to determine expression levels of transcripts, although their basic design is rather similar to GeneChip Exon 1.0 ST Arrays (exon arrays). Here, we show ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1110
更新日期:2012-03-01 00:00:00
abstract::We have studied the coil-to-helix transition of the DNA oligomer d(C4A4T4C4), using circular dichroism measurements to monitor the formation of A.T base pairs within the central self-complementary A4T4 region and the formation of protonated C.C+ base pairs at the ends of the oligomer. We found that both A.T and C.C+ b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.19.7565
更新日期:1984-10-11 00:00:00
abstract::While most of the recent improvements in multiple sequence alignment accuracy are due to better use of vertical information, which include the incorporation of consistency-based pairwise alignments and the use of profile alignments, we observe that it is possible to further improve accuracy by taking into account alig...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn945
更新日期:2009-02-01 00:00:00
abstract::The nature of mammalian origins of DNA replication remains controversial and this is primarily because two-dimensional gel replicon mapping techniques have identified broad zones of replication initiation whereas several other techniques, such as quantitative PCR, have disclosed more discrete sites of initiation at th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.10.2313
更新日期:1998-05-15 00:00:00
abstract::In Euplotes crassus millions of telomeres are synthesized during the sexual phase of the life cycle. Since these newly synthesized telomeres are longer than normal macronuclear telomeres, they must be trimmed to the mature size. We have examined the timing and mechanism of this trimming step. We have shown that a sudd...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.23.5366
更新日期:1993-11-25 00:00:00
abstract::The web software CHOP was developed to visualize the 'wobbling' in the third codon position of aligned DNA sequences. The simple features of this tool allow users to easily find regions suspected of containing coding sequences (CDSs). The program also allows visualization of the nucleotide diversity between two genomi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh448
更新日期:2004-07-01 00:00:00
abstract::The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bact...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1075
更新日期:2013-12-01 00:00:00
abstract::DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.6.e33
更新日期:2001-03-15 00:00:00
abstract::Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.23.4983
更新日期:1994-11-25 00:00:00