Abstract:
:Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibited the self-splicing of the sunY intron of phage T4 with a Ki of 8.5 microM (+/- 5 microM) and was active against other group I introns. Inhibition was found to be competitive with the substrate guanosine, unlike aminoglycoside antibiotics, which act non-competitively to inhibit the splicing of group I introns. Competitive inhibitors of group I intron splicing known to date all contain a guanidino group that was thought to be required for inhibition; lysinomicin lacks a guanidino group.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rogers J,Davies Jdoi
10.1093/nar/22.23.4983subject
Has Abstractpub_date
1994-11-25 00:00:00pages
4983-8issue
23eissn
0305-1048issn
1362-4962journal_volume
22pub_type
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journal_title:Nucleic acids research
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更新日期:1988-05-25 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2012-08-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2006-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1995-08-25 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa324
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1995-02-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2005-06-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1992-12-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:1998-12-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1182
更新日期:2008-03-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2008-01-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1993-02-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:1992-10-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:1980-08-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1999-09-15 00:00:00
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