Abstract:
:The 5' portions and flanking sequences of genes encoding types 1, 12, 24, and 6 M proteins were compared. Although the DNA sequences encoding the amino-termini of the mature M proteins had no obvious similarity, upstream sequences, and those encoding the signal peptides (leader sequences) of the four M protein genes had considerable similarity. In general, the 5' ends of all the leader sequences were more conserved than the 3' ends, although the M6 and M24 leader sequences had identical 3' ends. Sequence similarity among the deduced amino acid sequences of the four signal peptides was more extensive than the corresponding DNA sequences. We found that strict DNA similarity among all four sequences extended only to the ends of the hydrophilic amino-terminal regions of the signal peptides, but that amino acid sequence conservation continued to the ends of the respective hydrophobic cores. With the exception of the M6 and M24 sequences, the regions adjacent to the signal peptidase cleavage sites were highly variable.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Haanes-Fritz E,Kraus W,Burdett V,Dale JB,Beachey EH,Cleary Pdoi
10.1093/nar/16.10.4667subject
Has Abstractpub_date
1988-05-25 00:00:00pages
4667-77issue
10eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::In this study we describe a novel method to investigate the RNA-RNA interactions between a small RNA and its target that we termed 'RNA walk'. The method is based on UV-induced AMT cross-linking in vivo followed by affinity selection of the hybrid molecules and mapping the intermolecular adducts by RT-PCR or real-time...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp872
更新日期:2010-01-01 00:00:00
abstract::The effect of [Na+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stabili...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.6.759
更新日期:1975-06-01 00:00:00
abstract::The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonst...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.1.5
更新日期:1993-01-11 00:00:00
abstract::A multivariate statistical method called correspondence analysis was used to examine the codon usage of one-hundred-and-one nuclear genes of maize (Zea mays L.). Forty percent of the variation in codon usage was due to bias toward G or C-ending versus A or U-ending codons. Differences in levels of G-ending codons show...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.23.5294
更新日期:1993-11-25 00:00:00
abstract::Nam8, a component of yeast U1 snRNP, is optional for mitotic growth but required during meiosis, because Nam8 collaborates with Mer1 to promote splicing of essential meiotic mRNAs AMA1, MER2 and MER3. Here, we identify SPO22 and PCH2 as novel targets of Nam8-dependent meiotic splicing. Whereas SPO22 splicing is co-dep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1328
更新日期:2011-04-01 00:00:00
abstract::The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein id...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.1.131
更新日期:1985-01-11 00:00:00
abstract::RNA helicases of the DEAD-box protein family form the largest group of helicases. The human DEAD-box protein 1 (DDX1) plays an important role in tRNA and mRNA processing, is involved in tumor progression and is also hijacked by several virus families such as HIV-1 for replication and nuclear export. Although important...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv106
更新日期:2015-03-11 00:00:00
abstract::Brome mosaic virus (BMV) is a tripartite genome, positive-sense RNA virus of plants. Previously it was demonstrated that local hybridization between BMV RNAs (RNA-RNA heteroduplex formation) efficiently promotes non-homologous RNA recombination. In addition, studies on the role of the BMV polymerase in RNA recombinati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.8.1714
更新日期:2000-04-15 00:00:00
abstract::The molecular mechanisms that underlie the neurological manifestations of patients with inherited diseases of vitamin B12 (cobalamin) metabolism remain to date obscure. We observed transcriptomic changes of genes involved in RNA metabolism and endoplasmic reticulum stress in a neuronal cell model with impaired cobalam...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky634
更新日期:2018-09-06 00:00:00
abstract::Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs in response to recoding signals present in a minority of mRNAs. This 'read-through' is in competition with termination and is utilized for gene expression. One of the recoding signals known to stimulate read-through is a hexanucleotide sequence o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.9.2011
更新日期:2002-05-01 00:00:00
abstract::Transactivation by the ETS family of transcription factors, whose members share structurally conserved DNA-binding domains, is variably sensitive to methylation of their target genes. The mechanism by which DNA methylation controls ETS proteins remains poorly understood. Uncertainly also pervades the effects of hemi-m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw528
更新日期:2016-10-14 00:00:00
abstract::The molecular basis for the DNA binding specificity of the thyroid transcription factor 1 homeodomain (TTF-1HD) has been investigated. Methylation and ethylation interference experiments show that the TTF-1HD alone recapitulates the DNA binding properties of the entire protein. Studies carried out with mutant derivati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.15.3075
更新日期:1994-08-11 00:00:00
abstract::Mycobacterium tuberculosis (Mtb) Cmr (Rv1675c) is a CRP/FNR family transcription factor known to be responsive to cAMP levels and during macrophage infections. However, Cmr's DNA binding properties, cellular targets and overall role in tuberculosis (TB) complex bacteria have not been characterized. In this study, we u...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv889
更新日期:2016-01-08 00:00:00
abstract::The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic euryarchaeon Thermococcus aggregans. This motif plays a critical role in the balance between the synthesis and degradation of the DNA chain. Five different mutations of th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.20.3910
更新日期:2000-10-15 00:00:00
abstract::Transcriptional interference between adjacent genes has been demonstrated in a variety of biological systems. To study this process in RNA polymerase II (pol II) transcribed genes we have analysed the effect of transcription on tandem HIV-1 promoters integrated into the genome of HeLa cells. We show that transcription...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.5.1294
更新日期:1998-03-01 00:00:00
abstract::The lambdagt clones containing fragments of the Drosophila melanogaster genome were prepared and characterized by hybridization of their DNA with (I) lambdagt-cRNA; (2) lambdaC-cRNA; (3) Dm-cRNA; (4) the mRNA of D.melanogaster culture cells and (5) the stable cytoplasmic poly (A) RNA from the same source. The techniqu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.8.2115
更新日期:1976-08-01 00:00:00
abstract::The proportion of cytosines methylated in the DNA of nucleosome oligomers and of core particles appears indistinguishable from that of total nuclear DNA from CHO cells. However the DNA in nucleoprotein which is initially released from nuclei by treatment with very low levels of micrococcal nuclease and the first 10% o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.9.3097
更新日期:1977-09-01 00:00:00
abstract::The cis-requirements for the first editing site in the atp9 mRNA from pea mitochondria were investigated in an in vitro RNA editing system. Template RNAs deleted 5' of -20 are edited correctly, but with decreased efficiency. Deletions between -20 and the edited nucleotide abolish editing activity. Substitution of the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh763
更新日期:2004-08-04 00:00:00
abstract::Nuclei from frozen calf thymus suspended in buffer were analyzed for metal content prior to and after repeated washing. After three such extractions about 0.1 micrograms Zn/mg DNA and 0.025 micrograms Cu/mg DNA remained tightly associated with chromatin. This level of metal was essentially unchanged with subsequent wa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.21.5811
更新日期:1981-11-11 00:00:00
abstract::The RNA-Seq technology has revolutionized transcriptome characterization not only by accurately quantifying gene expression, but also by the identification of novel transcripts like chimeric fusion transcripts. The 'fusion' or 'chimeric' transcripts have improved the diagnosis and prognosis of several tumors, and have...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx315
更新日期:2017-07-27 00:00:00
abstract::Homo-oligomerization of proteins is abundant in nature, and is often intimately related with the physiological functions of proteins, such as in metabolism, signal transduction or immunity. Information on the homo-oligomer structure is therefore important to obtain a molecular-level understanding of protein functions ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx246
更新日期:2017-07-03 00:00:00
abstract::Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki486
更新日期:2005-07-01 00:00:00
abstract::Group II introns are self-splicing RNAs and retroelements found in bacteria and lower eukaryotic organelles. During the past several years, they have been uncovered in surprising numbers in bacteria due to the genome sequencing projects; however, most of the newly sequenced introns are not correctly identified. We hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg049
更新日期:2003-01-01 00:00:00
abstract::By transporting one DNA double helix (T-segment) through a double-strand break in another (G-segment), topoisomerase II reduces fractions of DNA catenanes, knots and supercoils to below equilibrium values. How DNA segments are selected to simplify the equilibrium DNA topology is enigmatic, and the biological relevance...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1037
更新日期:2014-02-01 00:00:00
abstract::The complete nucleotide sequences of the various precursor 5S RNA species occurring in Bacillus licheniformis have been elucidated. The B. licheniformis precursors contain a 5'-precursor-specific segment of 95 nucleotides which is four times as long as the corresponding segment of the p5S RNAs from the closely related...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.19.4535
更新日期:1980-10-10 00:00:00
abstract::An updated list of DNA sequences cloned from the human genome over the past year (1991) is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic probes comprising both gene and pseudogene sequences. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.suppl.2181
更新日期:1992-05-11 00:00:00
abstract::Prokaryotes evolved numerous systems that defend against predation by bacteriophages. In addition to well-known restriction-modification and CRISPR-Cas immunity systems, many poorly characterized systems exist. One class of such systems, named BREX, consists of a putative phosphatase, a methyltransferase and four othe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1125
更新日期:2019-01-10 00:00:00
abstract::In vitro selection of RNA-cleaving DNAzymes is a powerful method for isolating metal-specific DNA. A few successful examples are known, but it is still difficult to target some thiophilic metals such as Cd(2+) due to limited functional groups in DNA. While using modified bases expands the chemical functionality of DNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv519
更新日期:2015-07-13 00:00:00
abstract::mRNA deadenylation is under the control of cis-acting regulatory elements, which include AU-rich elements (AREs) and microRNA (miRNA) targeting sites, within the 3' untranslated region (3' UTRs) of eukaryotic mRNAs. Deadenylases promote miRNA-induced mRNA decay through their interaction with miRNA-induced silencing co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv959
更新日期:2015-12-15 00:00:00
abstract::Active DNA demethylation (ADDM) in mammals occurs via hydroxylation of 5-methylcytosine (5mC) by TET and/or deamination by AID/APOBEC family enzymes. The resulting 5mC derivatives are removed through the base excision repair (BER) pathway. At present, it is unclear how the cell manages to eliminate closely spaced 5mC ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw059
更新日期:2016-05-05 00:00:00