Abstract：
:Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs in response to recoding signals present in a minority of mRNAs. This 'read-through' is in competition with termination and is utilized for gene expression. One of the recoding signals known to stimulate read-through is a hexanucleotide sequence of the form CARYYA 3' adjacent to the stop codon. The present work finds that of the 91 unique viral sequences annotated as read-through, 90% had one of six of the 64 possible codons immediately 3' of the read-through stop codon. The relative efficiency of these read-through contexts in mammalian tissue culture cells has been determined using a dual luciferase fusion reporter. The relative importance of the identity of several individual nucleotides in the different hexanucleotides is complex.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Harrell L,Melcher U,Atkins JFdoi
10.1093/nar/30.9.2011keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
2011-7issue
9eissn
0305-1048issn
1362-4962journal_volume
30pub_type
杂志文章abstract：:Ribosome biogenesis (RiBi) is an extremely energy intensive process that is critical for gene expression. It is thus highly regulated, including through the tightly coordinated expression of over 200 RiBi genes by positive and negative transcriptional regulators. We investigated RiBi regulation as cells initiated meio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz231
更新日期：2019-06-04 00:00:00
abstract：:The identification and characterization of binding sites of DNA-binding molecules, including transcription factors (TFs), is a critical problem at the interface of chemistry, biology and molecular medicine. The Cognate Site Identification (CSI) array is a high-throughput microarray platform for measuring comprehensive...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn057
更新日期：2008-06-01 00:00:00
abstract：:The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.20.6393
更新日期：1982-10-25 00:00:00
abstract：:LRPPRC is a protein that has attracted interest both for its role in post-transcriptional regulation of mitochondrial gene expression and more recently because numerous mutated variants have been characterized as causing severe infantile mitochondrial neurodegeneration. LRPPRC belongs to the pentatricopeptide repeat (...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw575
更新日期：2016-08-19 00:00:00
abstract：:The control of messenger RNA (mRNA) function by micro RNAs (miRNAs) in animal cells requires the GW182 protein. GW182 is recruited to the miRNA repression complex via interaction with Argonaute protein, and functions downstream to repress protein synthesis. Interaction with Argonaute is mediated by GW/WG repeats, whic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq501
更新日期：2010-10-01 00:00:00
abstract：:The lytic-lysogenic decision in bacteriophage 186 is governed by the 186 CI repressor protein in a unique way. The 186 CI is proposed to form a wheel-like oligomer that can mediate either wrapped or looped nucleoprotein complexes to provide the cooperative and competitive interactions needed for regulation. Although c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt298
更新日期：2013-06-01 00:00:00
abstract：:The transcription factor Sox5 has previously been shown in chicken to be expressed in early neural crest cells and neural crest-derived peripheral glia. Here, we show in mouse that Sox5 expression also continues after neural crest specification in the melanocyte lineage. Despite its continued expression, Sox5 has litt...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn527
更新日期：2008-10-01 00:00:00
abstract：:To confirm the activity of an initial small molecule 'hit compound' from an activity screening, one needs to probe the structure-activity relationships by testing close analogs. The multi-fingerprint browser presented here (http://dcb-reymond23.unibe.ch:8080/MCSS/) enables one to rapidly identify such close analogs am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku379
更新日期：2014-07-01 00:00:00
abstract：:The restriction endonuclease (R.BsuFI) of Bacillus subtilis recognizes the target DNA sequence 5' CCGG. The R.BsuFI gene was found in close proximity to the cognate M.BsuFI gene, which had previously been characterized (1). Cloning of the R.BsuFI gene in E.coli was only possible with the M.BsuFI Mtase gene present on ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.23.6457
更新日期：1991-12-11 00:00:00
abstract：:The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.21.7607
更新日期：1985-11-11 00:00:00
abstract：:RaftProt (http://lipid-raft-database.di.uq.edu.au/) is a database of mammalian lipid raft-associated proteins as reported in high-throughput mass spectrometry studies. Lipid rafts are specialized membrane microdomains enriched in cholesterol and sphingolipids thought to act as dynamic signalling and sorting platforms....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1131
更新日期：2015-01-01 00:00:00
abstract：:We cloned and sequenced a portion of chloroplast DNA from the hornwort Anthoceros formosae. A nucleotide sequence of 7556 bp contained structures similar to those of ndhK, ndhC, trnV, trnM, atpE, atpB, rbcL, trnR and accD. The arrangement of these was the same as that of other chloroplast DNA. However, two nonsense co...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.6.1008
更新日期：1996-03-15 00:00:00
abstract：:CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq700
更新日期：2010-12-01 00:00:00
abstract：:The Aspergillus Genome Database (AspGD) is an online genomics resource for researchers studying the genetics and molecular biology of the Aspergilli. AspGD combines high-quality manual curation of the experimental scientific literature examining the genetics and molecular biology of Aspergilli, cutting-edge comparativ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp751
更新日期：2010-01-01 00:00:00
abstract：:The complete nucleotide sequence of the mitochondrial DNA of the amphioxus Branchiostoma lanceolatum has been determined. This mitochondrial genome is small (15 076 bp) because of the short size of the two rRNA genes and the tRNA genes. In addition, this genome contains a very short non-coding region (57 bp) with no s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.13.3279
更新日期：1998-07-01 00:00:00
abstract：:Simian cells have been transformed with SV40 origin-defective recombinant plasmids containing the tsA209 T-antigen gene. These plasmids contain deletions of either 5 or 52 nucleotides that include the BglI site at the SV40 ori, are defective for replication in COS-1 cells but retain a functional SV40 early promoter. T...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.22.7913
更新日期：1985-11-25 00:00:00
abstract：:Genome-wide association studies (GWAS) have become a preferred method to identify new genetic susceptibility loci. This technique aims to understanding the molecular etiology of common diseases, but in many cases, it has led to the identification of loci with no obvious biological relevance. Herein, we show that previ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks169
更新日期：2012-06-01 00:00:00
abstract：:In this paper we report the characterization of a newly sequenced 5' upstream region of the human HLA-DRA gene. We performed (i) search for transcription factor motifs, (ii) analysis of CpG display and observed/expected frequency ratios, (iii) search for regions homologous to the 5' upstream sequences of the murine EA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.10.1671
更新日期：1995-05-25 00:00:00
abstract：:Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.16.3121
更新日期：1996-08-15 00:00:00
abstract：:Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against natural transformatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt024
更新日期：2013-03-01 00:00:00
abstract：:The transfer ribonucleic acids (tRNAs) of B. subtilis at different growth phases are examined for changes in the composition and the methylation of minor constituents. The composition of the tRNAs indicates about equal amounts of adenosine and uridine, and of guanosine and cytidine. About 3-4 residues are present as m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1249
更新日期：1976-05-01 00:00:00
abstract：:The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains sev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.9.3063
更新日期：1985-05-10 00:00:00
abstract：:In small oocytes of Xenopus species, two sets of 5S RNA genes, oocyte-type and somatic-type, are fully activated. The 5S RNA transcripts are temporarily stored, half in association with TFIIIA to form a 7S particle, the other half in association with tRNA and two proteins (p48 and p43) to form a 42S particle. It has b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.21.8679
更新日期：1987-11-11 00:00:00
abstract：:We have examined the formation of alternate strand triple-helices at the target sequence A11(TC)6.(GA)6T11 using the oligonucleotides T11(AG)6 and T11(TG)6, by DNase I footprinting. These third strands were designed so as to form parallel T.AT triplets together with antiparallel G.GC and A.AT or T.AT triplets. We find...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.19.3977
更新日期：1994-09-25 00:00:00
abstract：:Phenotype analysis is commonly recognized to be of great importance for gaining insight into genetic interaction underlying inherited diseases. However, few computational contributions have been proposed for this purpose, mainly owing to lack of controlled clinical information easily accessible and structured for comp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki454
更新日期：2005-07-01 00:00:00
abstract：:Double stranded cDNA of cucumber pale fruit viroid ( CPFV ) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170 (1982] and the complete nucleotide sequence was established. The covalently closed circular molecules of single-stranded CPFV RNA consists of 303 nucleotides. The nucleotide sequence o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.8.3427
更新日期：1984-04-25 00:00:00
abstract：:Diastereomeric dithymidine methanephosphonamidates (TnpmT) were synthesized by reaction of 3'-amino-3'-deoxythymidine with 3'- O -acetylthymidin-5-yl-methanephosphonochloridate. Separated dinucleotide TnpmT(fast) and TnpmT(slow) diastereomers were used as building blocks to prepare chimeric dodecathy-midylates, posses...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.11.2650
更新日期：1998-06-01 00:00:00
abstract：:The Blocks Database contains multiple alignments of conserved regions in protein families which can be searched by e-mail (blocks@blocks.fhcrc.org) and World Wide Web (http://blocks.fhcrc.org/ ) servers to classify protein and nucleotide sequences. Recent enhancements to the servers include: (i) improved calculation o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.1.222
更新日期：1997-01-01 00:00:00
abstract：:Transfer ribonucleic acids were isolated from mitochondria of bovine heart and aminoacylated in vitro by a crude mitochondrial enzyme. Ser-tRNASerUCN and Ser-tRNASerAGY were isolated and characterized by partial sequencing. Although these tRNAs possess unique structural features not found in any bacterial tRNA, they f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.18.7175
更新日期：1986-09-25 00:00:00
abstract：:One of the most intensively studied chromatin binding factors is HP1α. HP1α is associated with silenced, heterochromatic regions of the genome and binds to H3K9me3. While H3K9me3 is necessary for HP1α recruitment to heterochromatin, it is becoming apparent that it is not sufficient suggesting that additional factors a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky632
更新日期：2018-10-12 00:00:00
