Abstract：
:The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains several sequences implicated in transcription initiation and termination in the yeast Saccharomyces cerevisiae. Although the methanol oxidase is translocated to the peroxisomes, no cleavable signal sequence was found at the N-terminus of the protein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ledeboer AM,Edens L,Maat J,Visser C,Bos JW,Verrips CT,Janowicz Z,Eckart M,Roggenkamp R,Hollenberg CPdoi
10.1093/nar/13.9.3063subject
Has Abstractpub_date
1985-05-10 00:00:00pages
3063-82issue
9eissn
0305-1048issn
1362-4962journal_volume
13pub_type
杂志文章abstract：:DnaD and DnaB are essential DNA-replication-initiation proteins in low-G+C content Gram-positive bacteria. Here we use sensitive Hidden Markov Model-based techniques to show that the DnaB and DnaD proteins share a common structure that is evident across all their structural domains, termed DDBH1 and DDBH2 (DnaD DnaB H...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq465
更新日期：2010-11-01 00:00:00
abstract：:Intron retention (IR) has been proposed to modulate the delay between transcription and translation. Here, we provide an exhaustive characterization of IR in differentiated white blood cells from both the myeloid and lymphoid lineage where we observed highest levels of IR in monocytes and B-cells, in addition to previ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1180
更新日期：2020-02-20 00:00:00
abstract：:SITEHOUND-web (http://sitehound.sanchezlab.org) is a binding-site identification server powered by the SITEHOUND program. Given a protein structure in PDB format SITEHOUND-web will identify regions of the protein characterized by favorable interactions with a probe molecule. These regions correspond to putative ligand...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp281
更新日期：2009-07-01 00:00:00
abstract：:Transcription factors (TFs) are sequence-specific DNA-binding proteins acting as critical regulators of gene expression. The Plant Promoter Analysis Navigator (PlantPAN; http://PlantPAN2.itps.ncku.edu.tw) provides an informative resource for detecting transcription factor binding sites (TFBSs), corresponding TFs, and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1035
更新日期：2016-01-04 00:00:00
abstract：:Several cDNA clones were isolated from cDNA libraries constructed with mRNA longer than 28S RNA from the murine cell line PYS-2/12. The plasmids have inserts containing 1-1.2 kb of the ribosomal 5' external transcribed spacer followed by nearly 700 nt of sequence for 18S rRNA and ending with a B2 element (retroposon)....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.4.671
更新日期：1992-02-25 00:00:00
abstract：:Interactions between proteins bound to distant sites along a DNA molecule require bending and twisting deformations in the intervening DNA. In certain systems, the sterically allowed protein-DNA and protein-protein interactions are hypothesized to produce loops with distinct geometries that may also be thermodynamical...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn389
更新日期：2008-07-01 00:00:00
abstract：:An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.20.4098
更新日期：1996-10-15 00:00:00
abstract：:In most eukaryotes, genetic exchange between paired homologs occurs in the context of a tripartite proteinaceous structure called the synaptonemal complex (SC). Genetic analyses have revealed that the genes encoding SC proteins are vital for meiotic chromosome pairing and recombination. However, the number, nature and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh559
更新日期：2004-04-28 00:00:00
abstract：:The oligomerization state and mode of binding to DNA of the GIY-YIG endonuclease II (EndoII) from bacteriophage T4 was studied using gel filtration and electrophoretic mobility shift assays with a set of mutants previously found to have altered enzyme activity. At low enzyme/DNA ratios all mutants except one bound to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp652
更新日期：2009-10-01 00:00:00
abstract：:Recently, we described a method for multiplex genome editing by natural transformation (MuGENT). Mutant constructs for MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro DNA splicing. In Vibrio cholerae, we uncover that this requirement is due to cytopl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx496
更新日期：2017-07-07 00:00:00
abstract：:Hepatitis C virus (HCV) is the major etiological agent of non-A, non-B hepatitis. Current therapies are not effective in all patients and can result in the generation of resistant mutants, leading to a need for new therapeutic options. HCV has an RNA genome that contains a well-defined and highly conserved secondary s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh706
更新日期：2004-07-19 00:00:00
abstract：:The sequences coding for DNA[cytosine-N4]methyltransferases MvaI (from Micrococcus varians RFL19) and Cfr9I (from Citrobacter freundii RFL9) have been determined. The predicted methylases are proteins of 454 and 300 amino acids, respectively. Primary structure comparison of M.Cfr9I and another m4C-forming methylase, M...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.23.9823
更新日期：1989-12-11 00:00:00
abstract：:The transcriptional potential of the hsp70 heat shock gene promoter is established prior to induction by stress. It has been shown previously that the TBP subunit of TFIID is associated with the TATA element and that RNA polymerase II is paused downstream from the transcription start site. In order to identify new int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.16.3327
更新日期：1995-08-25 00:00:00
abstract：:In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or curved) near the sites of cleavage by commonly-used restriction enzymes. One can use these plasmids to place any DNA reg...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.22.9447
更新日期：1989-11-25 00:00:00
abstract：:Ribonucleoprotein (RNP) granules are membraneless organelles (MLOs), which majorly consist of RNA and RNA-binding proteins and are formed via liquid-liquid phase separation (LLPS). Experimental studies investigating the drivers of LLPS have shown that intrinsically disordered proteins (IDPs) and nucleic acids like RNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1099
更新日期：2020-12-16 00:00:00
abstract：:Natural antisense transcripts are at least partially complementary to their sense transcripts. Cis-Sense/Antisense pairs (cis-SAs) have been extensively characterized and known to play diverse regulatory roles, whereas trans-Sense/Antisense pairs (trans-SAs) in animals are poorly studied. We identified long trans-SAs ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn470
更新日期：2008-09-01 00:00:00
abstract：:Plant viruses ubiquitously mediate the induction of miR168 trough the activities of viral suppressors of RNA silencing (VSRs) controlling the accumulation of ARGONAUTE1 (AGO1), one of the main components of RNA silencing based host defence system. Here we used a mutant Tombusvirus p19 VSR (p19-3M) disabled in its main...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt846
更新日期：2014-01-01 00:00:00
abstract：:The eukaryotic genome evolves under the dual constraint of maintaining coordinated gene transcription and performing effective DNA replication and cell division, the coupling of which brings about inevitable DNA topological tension. DNA supercoiling is resolved and, in some cases, even harnessed by the genome through ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx198
更新日期：2017-06-02 00:00:00
abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.24.5477
更新日期：1994-12-11 00:00:00
abstract：:The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks198
更新日期：2012-07-01 00:00:00
abstract：:DNA double-strand breaks (DSBs) are repaired by either the non-homologous end joining (NHEJ) or homologous recombination (HR) pathway. Pathway choice is determined by the generation of 3΄ single-strand DNA overhangs at the break that are initiated by the action of the Mre11-Rad50-Xrs2 (MRX) complex to direct repair to...
journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2017-06-02 00:00:00
abstract：:Prokaryotes evolved numerous systems that defend against predation by bacteriophages. In addition to well-known restriction-modification and CRISPR-Cas immunity systems, many poorly characterized systems exist. One class of such systems, named BREX, consists of a putative phosphatase, a methyltransferase and four othe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1125
更新日期：2019-01-10 00:00:00
abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh123
更新日期：2004-01-01 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) are, together with copy number variation, the primary source of variation in the human genome. SNPs are associated with altered response to drug treatment, susceptibility to disease and other phenotypic variation. Furthermore, during genetic screens for disease-associated mutatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm979
更新日期：2008-01-01 00:00:00
abstract：:Invariance of temporal order of genome replication in eukaryotic cells and its correlation with gene activity has been well-documented. However, recent data suggest a relax control of replication timing. To evaluate replication schedule accuracy, we detailed the replicational organization of the developmentally regula...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm586
更新日期：2007-01-01 00:00:00
abstract：:The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.11.2737
更新日期：1993-06-11 00:00:00
abstract：:MS²PIP is a data-driven tool that accurately predicts peak intensities for a given peptide's fragmentation mass spectrum. Since the release of the MS²PIP web server in 2015, we have brought significant updates to both the tool and the web server. In addition to the original models for CID and HCD fragmentation, we hav...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz299
更新日期：2019-07-02 00:00:00
abstract：UNLABELLED:The PubMed® database of biomedical citations allows the retrieval of scientific articles studying the function of chemicals in biology and medicine. Mining millions of available citations to search reported associations between chemicals and topics of interest would require substantial human time. We have im...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku432
更新日期：2014-07-01 00:00:00
abstract：:Characterization of the epigenetic status of individual cells remains a challenge. Current sequencing approaches have limited coverage, and it is difficult to assign an epigenetic status to the transcription state of individual gene alleles in the same cell. To address these limitations, a targeted microscopy-based ep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkab009
更新日期：2021-01-28 00:00:00
abstract：:MODPROPEP is a web server for knowledge-based modeling of protein-peptide complexes, specifically peptides in complex with major histocompatibility complex (MHC) proteins and kinases. The available crystal structures of protein-peptide complexes in PDB are used as templates for modeling peptides of desired sequence in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm266
更新日期：2007-07-01 00:00:00
