BREX system of Escherichia coli distinguishes self from non-self by methylation of a specific DNA site.

abstract：:Hammerhead ribozymes were transcribed from a dsDNA template containing four random nucleotides between stems II and III, which replace the naturally occurring GAA nucleotides. In vitro selection was used to select hammerhead ribozymes capable of in cis cleavage using denaturing polyacrylamide gels for the isolation of...

journal_title：Nucleic acids research

authors： Thomson JB,Sigurdsson ST,Zeuch A,Eckstein F

更新日期：1996-11-15 00:00:00

abstract：:We provide a novel web service, called rQuant.web, allowing convenient access to tools for quantitative analysis of RNA sequencing data. The underlying quantitation technique rQuant is based on quadratic programming and estimates different biases induced by library preparation, sequencing and read mapping. It can tack...

journal_title：Nucleic acids research

authors： Bohnert R,Rätsch G

更新日期：2010-07-01 00:00:00

abstract：:A set of new sulfurizing agents representing disulfides of arylsulfonic acids has been developed for the automated synthesis of phosphorothioate oligonucleotide analogues via the phosphoramidite method. These reagents, such as bis(benzenesulfonyl)disulfide, bis(p-toluenesulfonyl)disulfide, bis(p-methoxybenzensulfonyl)...

journal_title：Nucleic acids research

authors： Efimov VA,Kalinkina AL,Chakhmakhcheva OG,Hill TS,Jayaraman K

更新日期：1995-10-25 00:00:00

abstract：:For the first time mosaic nucleic acids composed of 50% RNA and 50% DNA can be obtained as transcripts with T7 RNA polymerase. Two NTPs could be replaced simultaneously in a transcription reaction. This means more than 40 deoxynucleotides were inserted in one transcript. Previously, a maximum of two deoxynucleotides c...

journal_title：Nucleic acids research

authors： Conrad F,Hanne A,Gaur RK,Krupp G

更新日期：1995-06-11 00:00:00

abstract：:Nucleosomes are the most abundant protein-DNA complexes in eukaryotes that provide compaction of genomic DNA and are implicated in regulation of transcription, DNA replication and repair. The details of DNA positioning on the nucleosome and the DNA conformation can provide key regulatory signals. Hydroxyl-radical foot...

journal_title：Nucleic acids research

authors： Shaytan AK,Xiao H,Armeev GA,Wu C,Landsman D,Panchenko AR

更新日期：2017-09-19 00:00:00

abstract：:Mono- and polyclonal antibodies directed against different domains of the potato leafroll luteovirus (PLRV) P1 (ORF1) protein were applied to the analysis of P1 expression during PLRV replication in planta. Western analyses detected P1 and a protein of approximately 25 kDa (P1-C25) that accumulated to readily detectab...

journal_title：Nucleic acids research

authors： Prüfer D,Kawchuk L,Monecke2 M,Nowok S,Fischer R,Rohde W

更新日期：1999-01-15 00:00:00

abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...

journal_title：Nucleic acids research

authors： Saintomé C,Amrane S,Mergny JL,Alberti P

更新日期：2016-04-07 00:00:00

abstract：:A normal human population has been screened for the existence of further restriction fragment length polymorphisms (RFLPs) in the clotting factor IX gene in addition to the TaqI polymorphism already characterised (1,2). Two polymorphic loci were found, both within 6 Kb of the TaqI polymorphism within the body of the f...

journal_title：Nucleic acids research

authors： Winship PR,Anson DS,Rizza CR,Brownlee GG

更新日期：1984-12-11 00:00:00

abstract：:To facilitate transcript mapping and to investigate alterations in genomic structure and gene expression in a defined genomic target, we developed a novel microarray-based method to detect transcriptional activity of the human chromosome 4q22-24 region. Loss of heterozygosity of human 4q22-24 is frequently observed in...

journal_title：Nucleic acids research

authors： Li LH,Li JC,Lin YF,Lin CY,Chen CY,Tsai SF

更新日期：2004-02-11 00:00:00

abstract：:X-linked agammaglobulinemia (XLA) is an immunodeficiency caused by mutations in the gene coding for Bruton's agammaglobulinemia tyrosine kinase (BTK). A database (BTKbase) of BTK mutations has been compiled and the recent update lists 368 entries from 318 unrelated families showing 228 unique molecular events. In addi...

journal_title：Nucleic acids research

authors： Vihinen M,Belohradsky BH,Haire RN,Holinski-Feder E,Kwan SP,Lappalainen I,Lehväslaiho H,Lester T,Meindl A,Ochs HD,Ollila J,Vorechovsky I,Weiss M,Smith CI

更新日期：1997-01-01 00:00:00

abstract：:Molecular replacement (MR) is the method of choice for X-ray crystallography structure determination when structural homologues are available in the Protein Data Bank (PDB). Although the success rate of MR decreases sharply when the sequence similarity between template and target proteins drops below 35% identical res...

journal_title：Nucleic acids research

authors： Claude JB,Suhre K,Notredame C,Claverie JM,Abergel C

更新日期：2004-07-01 00:00:00

abstract：:Codon usage bias is a universal feature of all genomes. Although codon usage has been shown to regulate mRNA and protein levels by influencing mRNA decay and transcription in eukaryotes, little or no genome-wide correlations between codon usage and mRNA levels are detected in mammalian cells, raising doubt on the sign...

journal_title：Nucleic acids research

authors： Yang Q,Lyu X,Zhao F,Liu Y

更新日期：2021-01-25 00:00:00

abstract：:Ribosome assembly is a process fundamental for all cellular activities. The efficiency and accuracy of the subunit assembly are tightly regulated and closely monitored. In the present work, we characterized, both compositionally and structurally, a set of in vivo 50S subunit precursors (45S), isolated from a mutant ba...

journal_title：Nucleic acids research

authors： Li N,Chen Y,Guo Q,Zhang Y,Yuan Y,Ma C,Deng H,Lei J,Gao N

更新日期：2013-08-01 00:00:00

abstract：:With the high number of sequences and structures streaming in from genomic projects, there is a need for more powerful and sophisticated annotation tools. Most problematic of the annotation efforts is predicting gene and protein function. Over the past few years there has been considerable progress in automated protei...

journal_title：Nucleic acids research

authors： Friedberg I,Harder T,Godzik A

更新日期：2006-07-01 00:00:00

abstract：:We report, herein, a new class of RNAi trigger molecules based on the unconventional parallel hybridization of two oligonucleotide chains. We have prepared and studied several parallel stranded (ps) duplexes, in which the parallel orientation is achieved through incorporation of isoguanine and isocytosine to form reve...

journal_title：Nucleic acids research

authors： Habibian M,Yahyaee-Anzahaee M,Lucic M,Moroz E,Martín-Pintado N,Di Giovanni LD,Leroux JC,Hall J,González C,Damha MJ

更新日期：2018-02-28 00:00:00

abstract：:In squid (Loligo bleekeri) mitochondria, the two 3'-terminal nucleotides (G72-G73) of the tRNA(Tyr) gene overlap with the two 5'-terminal nucleotides (G1-G2) of the downstream tRNA(Cys) gene. To elucidate the processing mechanism(s) of the tRNA molecules derived from this region, tRNAs were analyzed by sequencing cDNA...

journal_title：Nucleic acids research

authors： Tomita K,Ueda T,Watanabe K

更新日期：1996-12-15 00:00:00

abstract：:The HIV-1 viral infectivity factor (Vif) allows productive infection of non-permissive cells (including most natural HIV-1 targets) by counteracting the cellular cytosine deaminases APOBEC-3G (hA3G) and hA3F. The Vif-induced degradation of these restriction factors by the proteasome has been extensively studied, but l...

journal_title：Nucleic acids research

authors： Mercenne G,Bernacchi S,Richer D,Bec G,Henriet S,Paillart JC,Marquet R

更新日期：2010-01-01 00:00:00

abstract：:DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-ba...

journal_title：Nucleic acids research

authors： Ju J,Glazer AN,Mathies RA

更新日期：1996-03-15 00:00:00

abstract：:We have reported on the differences in site-specific cleavage between DNA and DNA-RNA hybrids by various prototypic DNA cleavers (accompanying paper). In the case of bleomycin (BLM), degradation at 5'-GC-3'sites was suppressed relative to the same sequence in double-stranded DNA, while 5'-GT-3' damage remained constan...

journal_title：Nucleic acids research

authors： Bansal M,Stubbe J,Kozarich JW

更新日期：1997-05-01 00:00:00

abstract：:The abundance and complexity of total poly(A)+RNA of human normal unstimulated lymphocytes, PHA-stimulated and leukemic lymphocytes were examined using the cDNA-poly(A)+ RNA hybridization technique. It was found that in both PHA-stimulated and leukemic lymphocytes the complexity of total poly(A)+RNA is strongly reduce...

journal_title：Nucleic acids research

authors： Torelli G,Ferrari S,Donelli A,Cadossi R,Ferrari S,Bosi P,Torelli U

更新日期：1981-12-21 00:00:00

abstract：:The unsymmetrical cyanine dye thiazole orange homodimer (TOTO) binds to single-stranded DNA (ssDNA, M13mp18 ssDNA) to form a fluorescent complex that is stable under the standard conditions of electrophoresis. The stability of this complex is indistinguishable from that of the corresponding complex of TOTO with double...

journal_title：Nucleic acids research

authors： Rye HS,Glazer AN

更新日期：1995-04-11 00:00:00

abstract：:Eucaryotic transcription initiation by RNA polymerase II involves protein:DNA interactions during the formation of a transcription complex. In addition to RNA polymerase II there are at least five other general transcription factors necessary for initiation with the adenovirus major late promoter. One of these, TFIIA,...

journal_title：Nucleic acids research

authors： Burke C,Yu XB,Marchitelli L,Davis EA,Ackerman S

更新日期：1990-06-25 00:00:00

abstract：:Unmethylated CpG rich islands are a feature of vertebrate DNA: they are associated with housekeeping and many tissue specific genes. CpG islands on the active X chromosome of mammals are also unmethylated. However, islands on the inactive X chromosome are heavily methylated. We have identified a CpG island in the 5' r...

journal_title：Nucleic acids research

authors： Alcalay M,Toniolo D

更新日期：1988-10-25 00:00:00

abstract：:Transfer RNAGlu4 was isolated from Drosophila melanogaster by affinity chromatography. The tRNA was iodinated "in vitro" with Na[25I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAGlu4 to the right arm of the second chro...

journal_title：Nucleic acids research

authors： Kubli E,Schmidt T

更新日期：1978-05-01 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures rapid degradation of mRNAs containing premature translation termination codons (PTCs) in eukaryotes. NMD has been shown to also regulate normal gene expression and thus emerged as one of the key post-transcriptional mechanisms ...

journal_title：Nucleic acids research

authors： Viegas MH,Gehring NH,Breit S,Hentze MW,Kulozik AE

更新日期：2007-01-01 00:00:00

abstract：:Plant orthologs of the yeast sucrose non-fermenting (Snf1) kinase and mammalian AMP-activated protein kinase (AMPK) represent an emerging class of important regulators of metabolic and stress signalling. The catalytic alpha-subunits of plant Snf1-related kinases (SnRKs) interact in the yeast two-hybrid system with dif...

journal_title：Nucleic acids research

authors： Ferrando A,Koncz-Kálmán Z,Farràs R,Tiburcio A,Schell J,Koncz C

更新日期：2001-09-01 00:00:00

abstract：:This article presents W-IQ-TREE, an intuitive and user-friendly web interface and server for IQ-TREE, an efficient phylogenetic software for maximum likelihood analysis. W-IQ-TREE supports multiple sequence types (DNA, protein, codon, binary and morphology) in common alignment formats and a wide range of evolutionary ...

journal_title：Nucleic acids research

authors： Trifinopoulos J,Nguyen LT,von Haeseler A,Minh BQ

更新日期：2016-07-08 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) Cmr (Rv1675c) is a CRP/FNR family transcription factor known to be responsive to cAMP levels and during macrophage infections. However, Cmr's DNA binding properties, cellular targets and overall role in tuberculosis (TB) complex bacteria have not been characterized. In this study, we u...

journal_title：Nucleic acids research

authors： Ranganathan S,Bai G,Lyubetskaya A,Knapp GS,Peterson MW,Gazdik M,C Gomes AL,Galagan JE,McDonough KA

更新日期：2016-01-08 00:00:00

abstract：:RNA structural complexity and flexibility present a challenge for computational modeling efforts. Experimental information and bioinformatics data can be used as restraints to improve the accuracy of RNA tertiary structure prediction. Regarding utilization of restraints, the fundamental questions are: (i) What is the ...

journal_title：Nucleic acids research

authors： Wang J,Williams B,Chirasani VR,Krokhotin A,Das R,Dokholyan NV

更新日期：2019-06-20 00:00:00

abstract：:U6 RNA is a member of a class of small abundant stable nuclear RNAs that are essential for splicing. In all species examined so far, the U6 RNA is a RNA polymerase III transcript. The U6 gene of the fission yeast Schizosaccharomyces pombe is unusual in that it is interrupted by an intron whose structure is similar to ...

journal_title：Nucleic acids research

authors： Potashkin J,Frendewey D

更新日期：1989-10-11 00:00:00