Single cell epigenetic visualization assay.

abstract：:Xenbase (www.xenbase.org), the model organism database for Xenopus laevis and X. (Silurana) tropicalis, is the principal centralized resource of genomic, development data and community information for Xenopus research. Recent improvements include the addition of the literature and interaction tabs to gene catalog page...

journal_title：Nucleic acids research

authors： Bowes JB,Snyder KA,Segerdell E,Jarabek CJ,Azam K,Zorn AM,Vize PD

更新日期：2010-01-01 00:00:00

abstract：:In this report we have analyzed the role of antisense transcription in the control of LEF1 transcription factor expression. A natural antisense transcript (NAT) is transcribed from a promoter present in the first intron of LEF1 gene and undergoes splicing in mesenchymal cells. Although this locus is silent in epitheli...

journal_title：Nucleic acids research

authors： Beltran M,Aparicio-Prat E,Mazzolini R,Millanes-Romero A,Massó P,Jenner RG,Díaz VM,Peiró S,de Herreros AG

更新日期：2015-07-13 00:00:00

abstract：:We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where b...

journal_title：Nucleic acids research

authors： Kojima T,Takei Y,Ohtsuka M,Kawarasaki Y,Yamane T,Nakano H

更新日期：2005-10-06 00:00:00

abstract：:A crucial step in analyzing mRNA-Seq data is to accurately and efficiently map hundreds of millions of reads to the reference genome and exon junctions. Here we present OLego, an algorithm specifically designed for de novo mapping of spliced mRNA-Seq reads. OLego adopts a multiple-seed-and-extend scheme, and does not ...

journal_title：Nucleic acids research

authors： Wu J,Anczuków O,Krainer AR,Zhang MQ,Zhang C

更新日期：2013-05-01 00:00:00

abstract：:Transcriptionally non-co-linear (NCL) transcripts can originate from trans-splicing (trans-spliced RNA; 'tsRNA') or cis-backsplicing (circular RNA; 'circRNA'). While numerous circRNAs have been detected in various species, tsRNAs remain largely uninvestigated. Here, we utilize integrative transcriptome sequencing of p...

journal_title：Nucleic acids research

authors： Chuang TJ,Chen YJ,Chen CY,Mai TL,Wang YD,Yeh CS,Yang MY,Hsiao YT,Chang TH,Kuo TC,Cho HH,Shen CN,Kuo HC,Lu MY,Chen YH,Hsieh SC,Chiang TW

更新日期：2018-04-20 00:00:00

abstract：:The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the...

journal_title：Nucleic acids research

authors： Tata PR,Tata NR,Kühl M,Sirbu IO

更新日期：2011-05-01 00:00:00

abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) offers a rich platform for data sharing, publishing and archiving and a globally comprehensive data set for onward use by the scientific community. With a broad scope spanning raw sequencing reads, genome assemblies and functional annotation, the resource...

journal_title：Nucleic acids research

authors： Toribio AL,Alako B,Amid C,Cerdeño-Tarrága A,Clarke L,Cleland I,Fairley S,Gibson R,Goodgame N,Ten Hoopen P,Jayathilaka S,Kay S,Leinonen R,Liu X,Martínez-Villacorta J,Pakseresht N,Rajan J,Reddy K,Rosello M,Silvester N

更新日期：2017-01-04 00:00:00

abstract：:Autosomal dominant and/or recessive progressive external ophthalmoplegia (ad/arPEO) is associated with mtDNA mutagenesis. It can be caused by mutations in three nuclear genes, encoding the adenine nucleotide translocator 1, the mitochondrial helicase Twinkle or DNA polymerase gamma (POLG). How mutations in these genes...

journal_title：Nucleic acids research

authors： Wanrooij S,Luoma P,van Goethem G,van Broeckhoven C,Suomalainen A,Spelbrink JN

更新日期：2004-06-04 00:00:00

abstract：:A circular denaturation and restriction map of mitochondrial DNA from Neurospora crassa is presented. The map shows the position of all twelve fragments produced by restriction endonuclease Eco R I and the position of the largest Hin III fragment along the previously established map of AT-rich sequences. The two wild ...

journal_title：Nucleic acids research

authors： Bernard U,Goldthwaite C,Küntzel H

更新日期：1976-11-01 00:00:00

abstract：:The cloned 18 S ribosomal RNA gene from Saccharomyces cerevisiae have been sequenced, using the Maxam-Gilbert procedure. From this data the complete sequence of 1789 nucleotides of the 18 S RNA was deduced. Extensive homology with many eucaryotic as well as E. coli ribosomal small subunit rRNA (S-rRNA) has been observ...

journal_title：Nucleic acids research

authors： Rubtsov PM,Musakhanov MM,Zakharyev VM,Krayev AS,Skryabin KG,Bayev AA

更新日期：1980-12-11 00:00:00

abstract：:Bulk replicative DNA synthesis in eukaryotes is highly accurate and efficient, primarily because of two DNA polymerases (Pols): Pols δ and ε. The high fidelity of these enzymes is due to their intrinsic base selectivity and proofreading exonuclease activity which, when coupled with post-replication mismatch repair, he...

journal_title：Nucleic acids research

authors： Korona DA,Lecompte KG,Pursell ZF

更新日期：2011-03-01 00:00:00

abstract：:Nucleosomes released from oviduct nuclei during brief micrococcal nuclease digestions are enriched in transcribed sequences (bloom K.S. and Anderson, J.N. (1978) Cell, 15, 141-150). Such nucleosomes released into this 1Sf supernatant fraction are enriched in proteins HMG14, 17 and a third lower molecular weight protei...

journal_title：Nucleic acids research

authors： Goodwin GH,Wright CA,Johns EW

更新日期：1981-06-25 00:00:00

abstract：:In addition to being a hallmark at active genes, histone variant H3.3 is deposited by ATRX at repressive chromatin regions, including the telomeres. It is unclear how H3.3 promotes heterochromatin assembly. We show that H3.3 is targeted for K9 trimethylation to establish a heterochromatic state enriched in trimethylat...

journal_title：Nucleic acids research

authors： Udugama M,M Chang FT,Chan FL,Tang MC,Pickett HA,R McGhie JD,Mayne L,Collas P,Mann JR,Wong LH

更新日期：2015-12-02 00:00:00

abstract：:The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and mai...

journal_title：Nucleic acids research

authors： Agostini F,Cirillo D,Bolognesi B,Tartaglia GG

更新日期：2013-01-07 00:00:00

abstract：:Three protein isoforms (74, 54 and 42 kDa) are expressed from repressor gene c in the Streptomyces temperate bacteriophage phiC31. Because expression of the two smaller isoforms, 54 and 42 kDa, is sufficient for superinfection immunity, the interaction between these isoforms was studied. The native 42 kDa repressor (N...

journal_title：Nucleic acids research

authors： Wilson SE,Smith MC

更新日期：1998-05-15 00:00:00

abstract：:Gene expression patterns have been demonstrated to be highly variable between similar cell types, for example lab strains and wild strains of Saccharomyces cerevisiae cultured under identical growth conditions exhibit a wide range of expression differences. We have used a genome-wide approach to characterize transcrip...

journal_title：Nucleic acids research

authors： Llinás M,Bozdech Z,Wong ED,Adai AT,DeRisi JL

更新日期：2006-02-21 00:00:00

abstract：:Highly regulated induction systems enabling dose-dependent and reversible fine-tuning of protein expression output are beneficial for engineering complex biosynthetic pathways. To address this, we developed PhiReX, a novel red/far-red light-regulated protein expression system for use in Saccharomyces cerevisiae. PhiRe...

journal_title：Nucleic acids research

authors： Hochrein L,Machens F,Messerschmidt K,Mueller-Roeber B

更新日期：2017-09-06 00:00:00

abstract：:Unoxidized crystalline silicon, characterized by high purity, high homogeneity, sturdiness and an atomically flat surface, offers many advantages for the construction of electronic miniaturized biosensor arrays upon attachment of biomolecules (DNA, proteins or small organic compounds). This allows to study the inciden...

journal_title：Nucleic acids research

authors： Cattaruzza F,Cricenti A,Flamini A,Girasole M,Longo G,Prosperi T,Andreano G,Cellai L,Chirivino E

更新日期：2006-02-28 00:00:00

abstract：:Recent effective use of TAL Effectors (TALEs) has provided an important approach to the design and synthesis of sequence-specific DNA-binding proteins. However, it is still a challenging task to design and manufacture effective TALE modulators because of the limited knowledge of TALE-DNA interactions. Here we synthesi...

journal_title：Nucleic acids research

authors： Zhang H,Li J,Hou S,Wang G,Jiang M,Sun C,Hu X,Zhuang F,Dai Z,Dai J,Xi JJ

更新日期：2014-08-01 00:00:00

abstract：:In addition to maintaining the GenBank(R) nucleic acid sequence database, the National Center for Biotechnology Information (NCBI) provides analysis and retrieval resources for the data in GenBank and other biological data available through NCBI's web site. NCBI resources include Entrez, the Entrez Programming Utiliti...

journal_title：Nucleic acids research

authors： Wheeler DL,Barrett T,Benson DA,Bryant SH,Canese K,Chetvernin V,Church DM,Dicuccio M,Edgar R,Federhen S,Feolo M,Geer LY,Helmberg W,Kapustin Y,Khovayko O,Landsman D,Lipman DJ,Madden TL,Maglott DR,Miller V,Ostell J,

更新日期：2008-01-01 00:00:00

abstract：:Several mono-, di-, tetra-, penta- and nonaribozymes were developed. These multitarget-ribozymes were targeted to cleave HIV-1 env RNA at up to nine different conserved sites. Each multitarget-ribozyme consisted of a chain of up to nine hammerhead motifs, each flanked by a different targeting sequence. The multitarget...

journal_title：Nucleic acids research

authors： Chen CJ,Banerjea AC,Harmison GG,Haglund K,Schubert M

更新日期：1992-09-11 00:00:00

abstract：:Functionality of the non-coding transcripts encoded by the human genome is the coveted goal of the modern genomics research. While commonly relied on the classical methods of forward genetics, integration of different genomics datasets in a global Systems Biology fashion presents a more productive avenue of achieving ...

journal_title：Nucleic acids research

authors： St Laurent G,Vyatkin Y,Antonets D,Ri M,Qi Y,Saik O,Shtokalo D,de Hoon MJ,Kawaji H,Itoh M,Lassmann T,Arner E,Forrest AR,FANTOM consortium.,Nicolas E,McCaffrey TA,Carninci P,Hayashizaki Y,Wahlestedt C,Kapranov P

更新日期：2016-04-20 00:00:00

abstract：:Equilibrative nucleoside transporters (ENTs) are a recently characterized and poorly understood group of membrane proteins that are important in the uptake of endogenous nucleosides required for nucleic acid and nucleoside triphosphate synthesis. Despite their central importance in cellular metabolism and nucleoside a...

journal_title：Nucleic acids research

authors： Sankar N,Machado J,Abdulla P,Hilliker AJ,Coe IR

更新日期：2002-10-15 00:00:00

abstract：:Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-stranded target DNA s...

journal_title：Nucleic acids research

authors： Roulon T,Coulaud D,Delain E,Le Cam E,Hélène C,Escudé C

更新日期：2002-02-01 00:00:00

abstract：:A complete nucleotide sequence of human aldolase B mRNA was determined with a recombinant cDNA (pHABL120-3). The cDNA insert was composed of 1,652 bases excluding poly(A) tail and the sequence was consistent with the previous results reported by others. However, S1 nuclease mapping and subsequent genomic analysis allo...

journal_title：Nucleic acids research

authors： Sakakibara M,Mukai T,Yatsuki H,Hori K

更新日期：1985-07-25 00:00:00

abstract：:Present results provide direct evidence of the nature of a conformational change in DNA when nucleosomes are formed from core histones and poly [d(A-T)]. First, we have found some features which have characteristic aspects of the A like conformation of DNA. Thus, an increased contribution due to a sugar conformation c...

journal_title：Nucleic acids research

authors： Brahms S,Brahmachari SK,Angelier N,Brahms JG

更新日期：1981-10-10 00:00:00

abstract：:In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how the different KH modules participate in the recognition of the RNA targets is necessary to dissect the way these protein...

journal_title：Nucleic acids research

authors： Hollingworth D,Candel AM,Nicastro G,Martin SR,Briata P,Gherzi R,Ramos A

更新日期：2012-08-01 00:00:00

abstract：:Mammalian cryptochromes (Crys) are essential circadian clock factors implicated in diverse clock-independent physiological functions, including DNA damage responses. Here we show that Cry1 modulates the ATR-mediated DNA damage checkpoint (DDC) response by interacting with Timeless (Tim) in a time-of-day-dependent mann...

journal_title：Nucleic acids research

authors： Kang TH,Leem SH

更新日期：2014-04-01 00:00:00

abstract：:Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...

journal_title：Nucleic acids research

authors： Hummel S,Meyerhof W,Korge E,Knöchel W

更新日期：1984-06-25 00:00:00

abstract：:Anisotropic motions of DNA fragments in the size range 6-118 base pairs are studied by the steady-state fluorescence polarization of different excitation transitions in the intercalated ethidium cation. Calculated effective tumbling and twisting times are found to be shorter than predicted for overall motions of rigid...

journal_title：Nucleic acids research

authors： Härd T,Kearns DR

更新日期：1986-05-12 00:00:00