Abstract:
:Nucleosomes released from oviduct nuclei during brief micrococcal nuclease digestions are enriched in transcribed sequences (bloom K.S. and Anderson, J.N. (1978) Cell, 15, 141-150). Such nucleosomes released into this 1Sf supernatant fraction are enriched in proteins HMG14, 17 and a third lower molecular weight protein which we show in this paper to be related to HMG14 and 17. This protein, which we call HMGY, runs as a doublet on polyacrylamide gels. A similar doublet is present in smaller quantities in chicken erythrocyte nuclei. Monomer nucleosomes in the 1SF supernatant have been separated by polyacrylamide gel electrophoresis into two main bands. The slower moving band contains the three HMG proteins HMG14, 17 and Y but lacks histone H1.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Goodwin GH,Wright CA,Johns EWdoi
10.1093/nar/9.12.2761subject
Has Abstractpub_date
1981-06-25 00:00:00pages
2761-75issue
12eissn
0305-1048issn
1362-4962journal_volume
9pub_type
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