Analysis of the RNA structural elements involved in the binding of the transcription factor III A from Xenopus laevis.

Abstract:

:Xenopus laevis 5S rRNA isolated from 7S particles or transcribed in vitro is found to adopt two alternative conformations. These two conformers contain different structural elements within the major TF III A binding domain, which, when isolated as RNA fragments, still interact with the transcription factor. Chemical modification of easily accessible adenines in their N-1 position does not have any measurable effect on the binding of 5S rRNA to TF III A. These observations are in support of the idea that only a small amount of conserved sequence information is required for the binding of the transcription factor, whereas specific secondary structure features seem to be essential.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Pieler T,Guddat U,Oei SL,Erdmann VA

doi

10.1093/nar/14.15.6313

subject

Has Abstract

pub_date

1986-08-11 00:00:00

pages

6313-26

issue

15

eissn

0305-1048

issn

1362-4962

journal_volume

14

pub_type

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