Abstract：
:Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo cell-free protein synthesis system shows a heterogeneous but equal distribution of these abundant translatable mRNAs in the different poly(A) size classes. By comparing the translational activity of inducible galactose-1-phosphate uridyl transferase mRNA, which can be monitored as a function of age, to contitutive mRNAs, we demonstrate that initially galactose-1-phosphate uridyl transferase mRNA has a uniformly large poly(A) tail which becomes heterogeneous and shorter with age in the cytoplasm. These observations are consistent with the previously observed cytoplasmic poly(A) catabolism in yeast and with cytoplasmic post-transcriptional modification of the poly(A) length of galactose-1-phosphate uridyl transferase mRNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Saunders CA,Bostian KA,Halvorson HOdoi
10.1093/nar/8.17.3841subject
Has Abstractpub_date
1980-09-11 00:00:00pages
3841-9issue
17eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract：:Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1225
更新日期：2020-02-28 00:00:00
abstract：:The Biodegradative Strain Database (BSD) is a freely-accessible, web-based database providing detailed information on degradative bacteria and the hazardous substances that they degrade, including corresponding literature citations, relevant patents and links to additional web-based biological and chemical data. The B...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg032
更新日期：2003-01-01 00:00:00
abstract：:Hepatocyte Nuclear Factor 4 (HNF-4), a liver-enriched orphan receptor of the nuclear receptor superfamily, is required for the expression of a wide variety of liver-specific genes including apoAI. To explore the possibility that site A of the apoAI gene enhancer might also be the target for HNF-4 without the interfere...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.25.5665
更新日期：1994-12-25 00:00:00
abstract：:The repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by the Tp1 family of highly repetitive retrotransposon-like sequences. Tp1 elements consist of two terminal direct repeats of 277bp which flank an internal domain of 8.3kb. They are the major sequence component in th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.2.279
更新日期：1991-01-25 00:00:00
abstract：:Targeted modulation of gene expression represents a valuable approach to understand the mechanisms governing gene regulation. In a therapeutic context, it can be exploited to selectively modify the aberrant expression of a disease-causing gene or to provide the target cells with a new function. Here, we have establish...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky171
更新日期：2018-05-18 00:00:00
abstract：:Fully modified 4'-thioDNA, an oligonucleotide only comprising 2'-deoxy-4'-thionucleosides, exhibited resistance to an endonuclease, in addition to preferable hybridization with RNA. Therefore, 4'-thioDNA is promising for application as a functional oligonucleotide. Fully modified 4'-thioDNA was found to behave like an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn011
更新日期：2008-04-01 00:00:00
abstract：:The complete nucleotide sequence of the coding region of foot and mouth disease virus RNA (strain A1061) is presented. The sequence extends from the primary initiation site, approximately 1200 nucleotide from the 5' end of the genome, in an open translational reading frame of 6,999 nucleotides to a termination codon 9...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.5.2461
更新日期：1984-03-12 00:00:00
abstract：:We have synthesized the tetradecamer GAGCXGATAACAAG containing a part of the sequence of the lactose operator. A guanine base in the sequence is replaced by the adduct of the carcinogen 2-acetylaminofluorene with guanine. Under the standard conditions of de-protection, the fluorene moiety is lost, leaving behind a gua...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.15.5093
更新日期：1983-08-11 00:00:00
abstract：:A frequently used method of comparing large RNA molecules employs the two-dimensional display of oligonucleotides generated through the action of specific RNases (oligonucleotide mapping, fingerprinting). Using computer simulations and simple analytic expressions the number of large RNase T1-resistant oligonucleotides...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.1.237
更新日期：1982-01-11 00:00:00
abstract：:Although mobility of the phylogenetically widespread group I introns appears to be mechanistically similar, the phage T4 intron-encoded endonucleases that promote mobility of the td and sunY introns are different from their eukaryotic counterparts. Most notably, they cleave at a distance from the intron insertion site...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.13.3763
更新日期：1990-07-11 00:00:00
abstract：:Inadequate cellular compartmentalisation of plasmid DNA and antisense oligodeoxynucleotides (ODNs) is generally considered as a major limitation in their use. In this study, an approach combining in situ visual-isation of rhodamine-labelled ODNs and affinity modification of proteins by radiolabelled-alkylating ODN der...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.11.2315
更新日期：1999-06-01 00:00:00
abstract：:Phosphorylation of the cellular single-stranded DNA-binding protein, replication protein A (RPA), occurs during normal mitotic cell cycle progression and also in response to genotoxic stress. In budding yeast, these reactions require the ATM homolog Mec1, a central regulator of the DNA replication and DNA damage check...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.23.4808
更新日期：2001-12-01 00:00:00
abstract：:MicroRNAs (miRNAs) can group together along the human genome to form stable secondary structures made of several hairpins hosting miRNAs in their stems. The few known examples of such structures are all involved in cancer development. A large scale computational analysis of human chromosomes crossing sequence analysis...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt112
更新日期：2013-04-01 00:00:00
abstract：:We have isolated a human cDNA (kup), encoding a new protein with two distantly spaced zinc fingers of the C2H2 type. This gene is highly conserved in mammals and is expressed mainly in hematopoietic cells and testis. Its expression was not higher in the various transformed cells tested than in the normal corresponding...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.7.1431
更新日期：1991-04-11 00:00:00
abstract：:Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.6.1552
更新日期：1999-03-15 00:00:00
abstract：:Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm) and poly (dC). The neutral form of pOLY (Ce) exhibits properties simi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.3.817
更新日期：1976-03-01 00:00:00
abstract：:Mature chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength are enriched in beta-globin DNA sequences. Vitellogenin chromatin, which is not expressed in this tissue, is found in aggregation prone, salt insoluble chromatin. There is a direct correlation between the size of soluble fragm...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.3.1081
更新日期：1987-02-11 00:00:00
abstract：:The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.20.6383
更新日期：1982-10-25 00:00:00
abstract：:We have isolated the 5'-end of the gene for the rat and human link protein by screening genomic libraries with oligonucleotides corresponding to the 5'-cDNA sequence. Several overlapping clones were isolated for the human link protein gene, while only one clone was obtained for the rat. All the clones contained a sing...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.8.1933
更新日期：1991-04-25 00:00:00
abstract：:The analysis of chromatin fine structure and transcription factor occupancy of differentially expressed genes by in vivo footprinting and ligation-mediated-PCR (LMPCR) is a powerful tool to understand the impact of chromatin on gene expression. However, as with all PCR-based techniques, the accuracy of the experiments...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1159
更新日期：2008-02-01 00:00:00
abstract：:DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restric...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.16.6711
更新日期：1986-08-26 00:00:00
abstract：:Genomic imbalances are a major cause of constitutional and acquired disorders. Therefore, aneuploidy screening has become the cornerstone of preimplantation, prenatal and postnatal genetic diagnosis, as well as a routine aspect of the diagnostic workup of many acquired disorders. Recently, array comparative genomic hy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl336
更新日期：2006-05-12 00:00:00
abstract：:Programmed chromosome breakage occurs at 50-200 specific sites in the genome of Tetrahymena thermo-phila during somatic nuclear (macronuclear) differentiation. Previous studies have identified a 15 bp sequence, the Cbs (for chromosome breakage sequence), that is necessary and sufficient to specify these sites. In this...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.4.895
更新日期：2000-02-15 00:00:00
abstract：:The three CD3 genes on human chromosome 11q23 encode proteins (gamma, delta and epsilon) which form part of the antigen receptor on T lymphocytes. All three genes are clustered within 50 kb and are activated approximately contemporaneously during the early stages of T cell ontogeny. In order to pinpoint potential regu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.3.459
更新日期：1990-02-11 00:00:00
abstract：:RNA co-transcriptional folding has long been suspected to play an active role in helping proper native folding of ribozymes and structured regulatory motifs in mRNA untranslated regions (UTRs). Yet, the underlying mechanisms and coding requirements for efficient co-transcriptional folding remain unclear. Traditional a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1036
更新日期：2007-01-01 00:00:00
abstract：:MLL, the trithorax ortholog, is a well-characterized histone 3 lysine 4 methyltransferase that is crucial for proper regulation of the Hox genes during embryonic development. Chromosomal translocations, disrupting the Mll gene, lead to aggressive leukemia with poor prognosis. However, the functions of MLL in cellular ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku458
更新日期：2014-07-01 00:00:00
abstract：:The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh531
更新日期：2004-04-23 00:00:00
abstract：:Single-nucleotide polymorphisms (SNPs) are often linked to critical phenotypes such as diseases or responses to vaccines, medications and environmental factors. However, the specific molecular mechanisms by which a causal SNP acts is usually not obvious. Changes in RNA secondary structure emerge as a possible explanat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1009
更新日期：2013-01-07 00:00:00
abstract：:The prokaryotic beta recombinase catalyzes site-specific recombination between two directly oriented minimal six sites in chromatin-integrated substrates. Here, we demonstrate that an enhanced green fluorescent protein (EGFP)-fused version of beta recombinase (beta-EGFP) is fully active, retaining most specific activi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnj001
更新日期：2006-01-03 00:00:00
abstract：:Mammalian antibody switch regions (∼1500 bp) are composed of a series of closely neighboring G4-capable sequences. Whereas numerous structural and genome-wide analyses of roles for minimal G4s in transcriptional regulation have been reported, Long G4-capable regions (LG4s)-like those at antibody switch regions-remain ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa357
更新日期：2020-06-19 00:00:00
