Abstract:
:We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit beta-globin gene. In most somatic tissues, including erythroid and non-erythroid tissues, about 50% of the DNA is resistant to cleavage at this site by HapII, though 100% cleavage is found with the isoschizomer MspI. Since the former enzyme is unable to cleave CCGG sites if the internal C residue is 5-methyl C (and since methylation is the only form of CpG modification documented in animal DNA), while the latter enzyme cleaves DNA irrespective of methylation at this residue, we infer that 50% of the CCGG sites in the beta-globin gene intron are methylated in these tissues. The same site appears to be 100% methylated (judged by the same criterium) in sperm DNA and about 80% methylated in brain DNA. DNA from the rabbit SIRC cell line is entirely unmethylated at this site.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Waalwijk C,Flavell RAdoi
10.1093/nar/5.12.4631subject
Has Abstractpub_date
1978-12-01 00:00:00pages
4631-4issue
12eissn
0305-1048issn
1362-4962journal_volume
5pub_type
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