The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability.

abstract：:The (cytosine-5) DNA methyltransferase M.HhaI causes its target cytosine base to be flipped completely out of the DNA helix upon binding. We have investigated the effects of replacing the target cytosine by other, mismatched bases, including adenine, guanine, thymine and uracil. We find that M.HhaI binds more tightly ...

journal_title：Nucleic acids research

authors： Klimasauskas S,Roberts RJ

更新日期：1995-04-25 00:00:00

abstract：:DNA double-strand breaks are repaired by end-joining or homologous recombination. A key-committing step of recombination is DNA end resection. In resection, phosphorylated CtIP first promotes the endonuclease of MRE11-RAD50-NBS1 (MRN). Subsequently, CtIP also stimulates the WRN/BLM-DNA2 pathway, coordinating thus both...

journal_title：Nucleic acids research

authors： Howard SM,Ceppi I,Anand R,Geiger R,Cejka P

更新日期：2020-06-04 00:00:00

abstract：:The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is an expertly curated database of literature-derived functional information for the model organism budding yeast, Saccharomyces cerevisiae. SGD constantly strives to synergize new types of experimental data and bioinformatics predictions with existin...

journal_title：Nucleic acids research

authors： Skrzypek MS,Nash RS,Wong ED,MacPherson KA,Hellerstedt ST,Engel SR,Karra K,Weng S,Sheppard TK,Binkley G,Simison M,Miyasato SR,Cherry JM

更新日期：2018-01-04 00:00:00

abstract：:With an increased emphasis on genotyping of single nucleotide polymorphisms (SNPs) in disease association studies, the genotyping platform of choice is constantly evolving. In addition, the development of more specific SNP assays and appropriate genotype validation applications is becoming increasingly critical to elu...

journal_title：Nucleic acids research

authors： Johnson MP,Haupt LM,Griffiths LR

更新日期：2004-03-26 00:00:00

abstract：:The thermal stability of the Tn10 encoded tetracycline resistance (TET) gene control region is investigated by melting studies using purified DNA restriction fragments containing various amounts of flanking sequences. In order to study the thermodynamic properties of this control region under conditions, where enough ...

journal_title：Nucleic acids research

authors： Hillen W,Unger B

更新日期：1982-04-24 00:00:00

abstract：:Organisms exposed to reactive oxygen species, generated endogenously during respiration or by environmental conditions, undergo oxidative stress. Stress response can either repair the damage or activate one of the programmed cell death (PCD) mechanisms, for example apoptosis, and finally end in cell death. One strikin...

journal_title：Nucleic acids research

authors： Mroczek S,Kufel J

更新日期：2008-05-01 00:00:00

abstract：:The DNA Data Bank of Japan (DDBJ) (http://www.ddbj.nig.ac.jp) has been providing public data services for thirty years (since 1987). We are collecting nucleotide sequence data from researchers as a member of the International Nucleotide Sequence Database Collaboration (INSDC, http://www.insdc.org), in collaboration wi...

journal_title：Nucleic acids research

authors： Mashima J,Kodama Y,Fujisawa T,Katayama T,Okuda Y,Kaminuma E,Ogasawara O,Okubo K,Nakamura Y,Takagi T

更新日期：2017-01-04 00:00:00

abstract：:The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initia...

journal_title：Nucleic acids research

authors： Sharp S,Dingermann T,Schaack J,Sharp JA,Burke DJ,DeRobertis EM,Söll D

更新日期：1983-12-20 00:00:00

abstract：:Regulation of mRNA translation is an important mechanism determining the level of expression of proteins in eukaryotic cells. Translation is most commonly initiated by cap-dependent scanning, but many eukaryotic mRNAs contain internal ribosome entry segments (IRESs), providing an alternative means of initiation capabl...

journal_title：Nucleic acids research

authors： Spriggs KA,Cobbold LC,Ridley SH,Coldwell M,Bottley A,Bushell M,Willis AE,Siddle K

更新日期：2009-09-01 00:00:00

abstract：:The 5' portions and flanking sequences of genes encoding types 1, 12, 24, and 6 M proteins were compared. Although the DNA sequences encoding the amino-termini of the mature M proteins had no obvious similarity, upstream sequences, and those encoding the signal peptides (leader sequences) of the four M protein genes h...

journal_title：Nucleic acids research

authors： Haanes-Fritz E,Kraus W,Burdett V,Dale JB,Beachey EH,Cleary P

更新日期：1988-05-25 00:00:00

abstract：:The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic euryarchaeon Thermococcus aggregans. This motif plays a critical role in the balance between the synthesis and degradation of the DNA chain. Five different mutations of th...

journal_title：Nucleic acids research

authors： Böhlke K,Pisani FM,Vorgias CE,Frey B,Sobek H,Rossi M,Antranikian G

更新日期：2000-10-15 00:00:00

abstract：:Genome-wide mapping in the identification of novel candidate genes has always been the standard method in genetics and genomics to correlate a clinically interesting phenotypic trait with a genotype. However, the performance of a mapping experiment using classical microsatellite approaches can be very time consuming. ...

journal_title：Nucleic acids research

authors： Klaften M,Hrabé de Angelis M

更新日期：2005-07-01 00:00:00

abstract：:A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site. The ability...

journal_title：Nucleic acids research

authors： Juillerat A,Dubois G,Valton J,Thomas S,Stella S,Maréchal A,Langevin S,Benomari N,Bertonati C,Silva GH,Daboussi F,Epinat JC,Montoya G,Duclert A,Duchateau P

更新日期：2014-04-01 00:00:00

abstract：:A physical mapping strategy has been developed to verify and accelerate the assembly and gap closure phase of a microbial genome shotgun-sequencing project. The protocol was worked out during the ongoing Pseudomonas putida KT2440 genome project. A macro-restriction map was constructed by linking probe hybridisation of...

journal_title：Nucleic acids research

authors： Weinel C,Tümmler B,Hilbert H,Nelson KE,Kiewitz C

更新日期：2001-11-15 00:00:00

abstract：:The Genomes On Line Database (GOLD) is a web resource for comprehensive access to information regarding complete and ongoing genome sequencing projects worldwide. The database currently incorporates information on over 1500 sequencing projects, of which 294 have been completed and the data deposited in the public data...

journal_title：Nucleic acids research

authors： Liolios K,Tavernarakis N,Hugenholtz P,Kyrpides NC

更新日期：2006-01-01 00:00:00

abstract：:We describe the structure (3840 bp) of a novel Euglena gracilis chloroplast ribosomal protein operon that encodes the five genes rpl16-rpl14-rpl5-rps8-rpl36. The gene organization resembles the spc and the 3'-end of the S10 ribosomal protein operons of E. coli. The rpl5 is a new chloroplast gene not previously reporte...

journal_title：Nucleic acids research

authors： Christopher DA,Hallick RB

更新日期：1989-10-11 00:00:00

abstract：:The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and ha...

journal_title：Nucleic acids research

authors： Martens AT,Taylor J,Hilser VJ

更新日期：2015-04-20 00:00:00

abstract：:Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from the cytoplasm to the nucleus have been studied in yeast, the rat hepatoma and human cells. In the current study, we investigated tRNA degradation and accumulation in HeLa cells under various forms of stress. We found that i...

journal_title：Nucleic acids research

authors： Watanabe K,Miyagawa R,Tomikawa C,Mizuno R,Takahashi A,Hori H,Ijiri K

更新日期：2013-04-01 00:00:00

abstract：:The miRNA Registry provides a service for the assignment of miRNA gene names prior to publication. A comprehensive and searchable database of published miRNA sequences is accessible via a web interface (http://www.sanger.ac.uk/Software/Rfam/mirna/), and all sequence and annotation data are freely available for downloa...

journal_title：Nucleic acids research

authors： Griffiths-Jones S

更新日期：2004-01-01 00:00:00

abstract：:We have implemented a genome annotation system for prokaryotes called AGMIAL. Our approach embodies a number of key principles. First, expert manual annotators are seen as a critical component of the overall system; user interfaces were cyclically refined to satisfy their needs. Second, the overall process should be o...

journal_title：Nucleic acids research

authors： Bryson K,Loux V,Bossy R,Nicolas P,Chaillou S,van de Guchte M,Penaud S,Maguin E,Hoebeke M,Bessières P,Gibrat JF

更新日期：2006-07-19 00:00:00

abstract：:An important challenge in cancer genomics is precise detection of structural variations (SVs) by high-throughput short-read sequencing, which is hampered by the high false discovery rates of existing analysis tools. Here, we propose an accurate SV detection method named COSMOS, which compares the statistics of the map...

journal_title：Nucleic acids research

authors： Yamagata K,Yamanishi A,Kokubu C,Takeda J,Sese J

更新日期：2016-05-05 00:00:00

abstract：:Although mobility of the phylogenetically widespread group I introns appears to be mechanistically similar, the phage T4 intron-encoded endonucleases that promote mobility of the td and sunY introns are different from their eukaryotic counterparts. Most notably, they cleave at a distance from the intron insertion site...

journal_title：Nucleic acids research

authors： Bell-Pedersen D,Quirk S,Clyman J,Belfort M

更新日期：1990-07-11 00:00:00

abstract：:Rad51 protein promotes homologous recombination in eukaryotes. Recombination activities are activated by Rad51 filament assembly on ssDNA. Previous studies of yeast Rad51 showed that His352 occupies an important position at the filament interface, where it could relay signals between subunits and active sites. To inve...

journal_title：Nucleic acids research

authors： Chen J,Villanueva N,Rould MA,Morrical SW

更新日期：2010-08-01 00:00:00

abstract：:EcoGene (http://ecogene.org) is a database and website devoted to continuously improving the structural and functional annotation of Escherichia coli K-12, one of the most well understood model organisms, represented by the MG1655(Seq) genome sequence and annotations. Major improvements to EcoGene in the past decade i...

journal_title：Nucleic acids research

authors： Zhou J,Rudd KE

更新日期：2013-01-01 00:00:00

abstract：:Transfer ribonucleic acids were isolated from mitochondria of bovine heart and aminoacylated in vitro by a crude mitochondrial enzyme. Ser-tRNASerUCN and Ser-tRNASerAGY were isolated and characterized by partial sequencing. Although these tRNAs possess unique structural features not found in any bacterial tRNA, they f...

journal_title：Nucleic acids research

authors： Gebhardt-Singh E,Sprinzl M

更新日期：1986-09-25 00:00:00

abstract：:The heterodimeric subunit, SRP9/14, of the signal recognition particle (SRP) has previously been found to bind to scAlu and scB1 RNAs in vitro and to exist in large excess over SRP in anthropoid cells. Here we show that human and mouse SRP9/14 bind with high affinities to other Alu-like RNAs of different evolutionary ...

journal_title：Nucleic acids research

authors： Bovia F,Wolff N,Ryser S,Strub K

更新日期：1997-01-15 00:00:00

abstract：:DNA polymerases alpha and delta are essential enzymes believed to play critical roles in initiation and replication of chromosome DNA. In this study, we show that the genes for Schizosaccharomyces pombe (S.pombe) DNA polymerase alpha and delta (pol alpha+ and pol delta+) are essential for cell viability. Disruption of...

journal_title：Nucleic acids research

authors： Francesconi S,Park H,Wang TS

更新日期：1993-08-11 00:00:00

abstract：:The positive role of PARP1 in regulation of various nuclear DNA transactions is well established. Although a mitochondrial localization of PARP1 has been suggested, its role in the maintenance of the mitochondrial DNA is currently unknown. Here we investigated the role of PARP1 in the repair of the mitochondrial DNA i...

journal_title：Nucleic acids research

authors： Szczesny B,Brunyanszki A,Olah G,Mitra S,Szabo C

更新日期：2014-12-01 00:00:00

abstract：:Cellular ribonucleic acid (RNA) plays a crucial role in the initial conversion of cellular prion protein PrP(C) to infectious PrP(Sc) or scrapie. The nature of this RNA remains elusive. Previously, RNA aptamers against PrP(C) have been isolated and found to form G-quadruplexes (G4s). PrP(C) binding to G4 RNAs destabil...

journal_title：Nucleic acids research

authors： Olsthoorn RC

更新日期：2014-08-01 00:00:00

abstract：:Gene expression patterns have been demonstrated to be highly variable between similar cell types, for example lab strains and wild strains of Saccharomyces cerevisiae cultured under identical growth conditions exhibit a wide range of expression differences. We have used a genome-wide approach to characterize transcrip...

journal_title：Nucleic acids research

authors： Llinás M,Bozdech Z,Wong ED,Adai AT,DeRisi JL

更新日期：2006-02-21 00:00:00