The internal region of CtIP negatively regulates DNA end resection.

abstract：:The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins. Here we describe the use of an RNA aptamer, scaffolded to a ...

journal_title：Nucleic acids research

authors： Iioka H,Loiselle D,Haystead TA,Macara IG

更新日期：2011-04-01 00:00:00

abstract：:We describe an interactive computer program (ANALYSEQ) that is used from a simple graphics terminal. The main purpose of the program is to determine the function of nucleic acid sequences but it also offers the simpler listing, searching and counting options. It contains methods to locate genes by looking for the effe...

journal_title：Nucleic acids research

authors： Staden R

更新日期：1984-01-11 00:00:00

abstract：:TORQUE is a tool for cross-species querying of protein-protein interaction networks. It aims to answer the following question: given a set of proteins constituting a known complex or a pathway in one species, can a similar complex or pathway be found in the protein network of another species? To this end, Torque seeks...

journal_title：Nucleic acids research

authors： Bruckner S,Hüffner F,Karp RM,Shamir R,Sharan R

更新日期：2009-07-01 00:00:00

abstract：:Using peptide arrays and binding to native histone proteins, we show that the ADD domain of Dnmt3a specifically interacts with the H3 histone 1-19 tail. Binding is disrupted by di- and trimethylation of K4, phosphorylation of T3, S10 or T11 and acetylation of K4. We did not observe binding to the H4 1-19 tail. The ADD...

journal_title：Nucleic acids research

authors： Zhang Y,Jurkowska R,Soeroes S,Rajavelu A,Dhayalan A,Bock I,Rathert P,Brandt O,Reinhardt R,Fischle W,Jeltsch A

更新日期：2010-07-01 00:00:00

abstract：:The editing function of aminoacyl-tRNA synthetases (aaRSs) is indispensible for formation of the correct aminoacyl-tRNAs. Editing deficiency may lead to growth inhibition and the pathogenesis of various diseases. Herein, we confirmed that norvaline (Nva) but not isoleucine or valine is the major threat to the editing ...

journal_title：Nucleic acids research

authors： Ji QQ,Fang ZP,Ye Q,Chi CW,Wang ED

更新日期：2017-07-07 00:00:00

abstract：:DIAL (dihedral alignment) is a web server that provides public access to a new dynamic programming algorithm for pairwise 3D structural alignment of RNA. DIAL achieves quadratic time by performing an alignment that accounts for (i) pseudo-dihedral and/or dihedral angle similarity, (ii) nucleotide sequence similarity a...

journal_title：Nucleic acids research

authors： Ferrè F,Ponty Y,Lorenz WA,Clote P

更新日期：2007-07-01 00:00:00

abstract：:Phage_Finder, a heuristic computer program, was created to identify prophage regions in completed bacterial genomes. Using a test dataset of 42 bacterial genomes whose prophages have been manually identified, Phage_Finder found 91% of the regions, resulting in 7% false positive and 9% false negative prophages. A searc...

journal_title：Nucleic acids research

authors： Fouts DE

更新日期：2006-01-01 00:00:00

abstract：:RNA can directly bind to purine-rich DNA via Hoogsteen base pairing, forming a DNA:RNA triple helical structure that anchors the RNA to specific sequences and allows guiding of transcription regulators to distinct genomic loci. To unravel the prevalence of DNA:RNA triplexes in living cells, we have established a fast ...

journal_title：Nucleic acids research

authors： Sentürk Cetin N,Kuo CC,Ribarska T,Li R,Costa IG,Grummt I

更新日期：2019-03-18 00:00:00

abstract：:A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 ...

journal_title：Nucleic acids research

authors： Visser L,Reemst AC,van Mansfeld AD,Rozijn TH

更新日期：1982-04-10 00:00:00

abstract：:Here, we present an update of Breast Cancer Now Tissue Bank bioinformatics, a rich platform for the sharing, mining, integration and analysis of breast cancer data. Its modalities provide researchers with access to a centralised information gateway from which they can access a network of bioinformatic resources to que...

journal_title：Nucleic acids research

authors： Gadaleta E,Pirrò S,Dayem Ullah AZ,Marzec J,Chelala C

更新日期：2018-01-04 00:00:00

abstract：:Single-nucleotide variation and gene expression of disease samples represent important resources for biomarker discovery. Many databases have been built to host and make available such data to the community, but these databases are frequently limited in scope and/or content. BioMuta, a database of cancer-associated si...

journal_title：Nucleic acids research

authors： Dingerdissen HM,Torcivia-Rodriguez J,Hu Y,Chang TC,Mazumder R,Kahsay R

更新日期：2018-01-04 00:00:00

abstract：:The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residue...

journal_title：Nucleic acids research

authors： Yang CL,Chang LS,Zhang P,Hao H,Zhu L,Toomey NL,Lee MY

更新日期：1992-02-25 00:00:00

abstract：:WorfDB (Worm ORFeome DataBase; http://worfdb.dfci.harvard.edu) was created to integrate and disseminate the data from the cloning of complete set of approximately 19 000 predicted protein-encoding Open Reading Frames (ORFs) of Caenorhabditis elegans (also referred to as the 'worm ORFeome'). WorfDB serves as a central ...

journal_title：Nucleic acids research

authors： Vaglio P,Lamesch P,Reboul J,Rual JF,Martinez M,Hill D,Vidal M

更新日期：2003-01-01 00:00:00

abstract：:Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced sile...

journal_title：Nucleic acids research

authors： Zhang J,Liu H,Yao Q,Yu X,Chen Y,Cui R,Wu B,Zheng L,Zuo J,Huang Z,Ma J,Gan J

更新日期：2016-11-02 00:00:00

abstract：:The two genes encoding the class IIS restriction-modification system MboII from Moraxella bovis were cloned separately in two compatible plasmids and expressed in E. coli RR1 delta M15. The nucleotide sequences of the MboII endonuclease (R.MboII) and methylase (M.MboII) genes were determined and the putative start cod...

journal_title：Nucleic acids research

authors： Bocklage H,Heeger K,Müller-Hill B

更新日期：1991-03-11 00:00:00

abstract：:The dominant kanr marker gene plays an important role in gene disruption experiments in budding yeast, as this marker can be used in a variety of yeast strains lacking the conventional yeast markers. We have developed a loxP-kanMX-loxP gene disruption cassette, which combines the advantages of the heterologous kanr ma...

journal_title：Nucleic acids research

authors： Güldener U,Heck S,Fielder T,Beinhauer J,Hegemann JH

更新日期：1996-07-01 00:00:00

abstract：:The interaction of Hoechst 33258 with the minor groove of the adenine-tract DNA duplex d(CTTTTGCAAAAG)2 has been studied in both D2O and H2O solutions by 1D and 2D 1H NMR spectroscopy. Thirty-one nuclear Overhauser effects between drug and nucleotide protons within the minor groove of the duplex, together with ring-cu...

journal_title：Nucleic acids research

authors： Searle MS,Embrey KJ

更新日期：1990-07-11 00:00:00

abstract：:Nuclear factor I (NFI) is a site-specific DNA binding protein required for the replication of adenovirus type 2 DNA in vitro and in vivo. To study sequence requirements for the interaction of NFI with DNA, we have measured the binding of the protein to a variety of synthetic sites. Binding sites for NFI (FIB sites) we...

journal_title：Nucleic acids research

authors： Gronostajski RM

更新日期：1987-07-24 00:00:00

abstract：:TATA-binding protein (TBP) is central to the regulation of eukaryotic transcription initiation. Recruitment of TBP to target genes can be positively regulated by one of two basal transcription factor complexes: SAGA or TFIID. Negative regulation of TBP promoter association can be performed by Mot1p or the NC2 complex....

journal_title：Nucleic acids research

authors： Spedale G,Meddens CA,Koster MJ,Ko CW,van Hooff SR,Holstege FC,Timmers HT,Pijnappel WW

更新日期：2012-02-01 00:00:00

abstract：:The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initia...

journal_title：Nucleic acids research

authors： Sharp S,Dingermann T,Schaack J,Sharp JA,Burke DJ,DeRobertis EM,Söll D

更新日期：1983-12-20 00:00:00

abstract：:The model mRNA (MR), 11-mer RNA containing two nitroxide spin labels at the 5'- and 3'-terminal nucleotides and prone to form a stable homodimer (MR)2, was used for Electron Paramagnetic Resonance study of structural rearrangements in mRNA occurring upon its binding to human 80S ribosomes. The formation of two differe...

journal_title：Nucleic acids research

authors： Malygin AA,Graifer DM,Meschaninova MI,Venyaminova AG,Timofeev IO,Kuzhelev AA,Krumkacheva OA,Fedin MV,Karpova GG,Bagryanskaya EG

更新日期：2018-01-25 00:00:00

abstract：:Structured RNA molecules form complex 3D architectures stabilized by multiple interactions involving the nucleotide base, sugar and phosphate moieties. A significant percentage of the bases in structured RNA molecules in the Protein Data Bank (PDB) hydrogen-bond with phosphates of other nucleotides. By extracting and ...

journal_title：Nucleic acids research

authors： Zirbel CL,Sponer JE,Sponer J,Stombaugh J,Leontis NB

更新日期：2009-08-01 00:00:00

abstract：:The available reagents for the attachment of functional moieties to plasmid DNA are limiting. Most reagents bind plasmid DNA in a non-sequence- specific manner, with undefined stoichiometry, and affect DNA charge and delivery properties or involve chemical modifications that abolish gene expression. The design and abi...

journal_title：Nucleic acids research

authors： Hertoghs KM,Ellis JH,Catchpole IR

更新日期：2003-10-15 00:00:00

abstract：:MicroRNAs (miRNAs) are approximately 22 nucleotide (nt) long and play important roles in post-transcriptional regulation in both plants and animals. In animals, precursor (pre-) miRNAs are ∼70 nt hairpins produced by Drosha cleavage of long primary (pri-) miRNAs in the nucleus. Exportin-5 (XPO5) transports pre-miRNAs ...

journal_title：Nucleic acids research

authors： Sheng P,Fields C,Aadland K,Wei T,Kolaczkowski O,Gu T,Kolaczkowski B,Xie M

更新日期：2018-06-20 00:00:00

abstract：:Most type II restriction-modification (R-M) systems produce separate endonuclease (REase) and methyltransferase (MTase) proteins. After R-M genes enter a new cell, MTase activity must appear before REase or the host chromosome will be cleaved. Temporal control of these genes thus has life-or-death consequences. PvuII ...

journal_title：Nucleic acids research

authors： Mruk I,Blumenthal RM

更新日期：2009-02-01 00:00:00

abstract：:Altering zinc bioavailability to bacterial pathogens is a key component of host innate immunity. Thus, the ability to sense and adapt to the alterations in zinc concentrations is critical for bacterial survival and pathogenesis. To understand the adaptive responses of group A Streptococcus (GAS) to zinc limitation and...

journal_title：Nucleic acids research

authors： Sanson M,Makthal N,Flores AR,Olsen RJ,Musser JM,Kumaraswami M

更新日期：2015-01-01 00:00:00

abstract：:cDNA expression libraries displayed on lambda phage have been successfully employed to identify partners involved in antibody-antigen, protein- protein and DNA-protein interactions and represent a novel approach to functional genomics. However, as in all other cDNA expression libraries based on fusion to a carrier pol...

journal_title：Nucleic acids research

authors： Ansuini H,Cicchini C,Nicosia A,Tripodi M,Cortese R,Luzzago A

更新日期：2002-08-01 00:00:00

abstract：:A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amin...

journal_title：Nucleic acids research

authors： Graham RW,Atkinson T,Kilburn DG,Miller RC Jr,Warren RA

更新日期：1993-10-25 00:00:00

abstract：:The crp gene of E. coli, which codes for cAMP receptor protein (CRP), has been cloned in the plasmid pBR322 on the basis of a genetic complementation. One of the recombinant plasmids, pHA1, was shown to direct the synthesis of CRP in a cell-free system. The location of the crp gene was determined by constructing subcl...

journal_title：Nucleic acids research

authors： Aiba H,Fujimoto S,Ozaki N

更新日期：1982-02-25 00:00:00

abstract：:Over the past 10 years, fluorescent end-labeling of DNA fragments has evolved into the preferred method of DNA detection for a wide variety of applications, including DNA sequencing and PCR fragment analysis. One of the advantages inherent in fluorescent detection methods is the ability to perform multi-color analyses...

journal_title：Nucleic acids research

authors： Tu O,Knott T,Marsh M,Bechtol K,Harris D,Barker D,Bashkin J

更新日期：1998-06-01 00:00:00