Chromatin methylation activity of Dnmt3a and Dnmt3a/3L is guided by interaction of the ADD domain with the histone H3 tail.

abstract：:Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...

journal_title：Nucleic acids research

authors： Perales-Clemente E,Fernández-Silva P,Acín-Pérez R,Pérez-Martos A,Enríquez JA

更新日期：2011-01-01 00:00:00

abstract：:The entire intervening sequence of Tetrahymena thermophila ribosomal DNA has been determined. It is 413 nucleotides long and has the same splice junctions as those in T. pigmentosa. There is 93% homology between the intervening sequences in the two species, and 100% homology between their adjacent 26S RNA coding regio...

journal_title：Nucleic acids research

authors： Kan NC,Gall JG

更新日期：1982-05-11 00:00:00

abstract：:FANTOM DB, the database of Functional Annotation of RIKEN Mouse cDNA Clones, is designed to store sequence information of RIKEN full-length enriched mouse cDNA clones, graphical views of sequence analysis results, curated functional annotation information and additional descriptions, including Gene Ontology terms. RIK...

journal_title：Nucleic acids research

authors： Bono H,Kasukawa T,Furuno M,Hayashizaki Y,Okazaki Y

更新日期：2002-01-01 00:00:00

abstract：:Termination of protein synthesis is promoted in ribosomes by proper stop codon discrimination by class 1 polypeptide release factors (RFs). A large set of prokaryotic RFs differing in stop codon specificity, RF1 for UAG and UAA, and RF2 for UGA and UAA, was analyzed by means of a recently developed computational metho...

journal_title：Nucleic acids research

authors： Oparina NJ,Kalinina OV,Gelfand MS,Kisselev LL

更新日期：2005-09-14 00:00:00

abstract：:Human transcription factor Sp1 contains three contiguous repeats of the C2H2-type zinc finger motif and binds to the decanucleotide sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box). In order to determine whether the three-zinc finger peptide Sp1(530-623) has selectivity for sequence outside the GC box, we used a sel...

journal_title：Nucleic acids research

authors： Nagaoka M,Shiraishi Y,Sugiura Y

更新日期：2001-12-15 00:00:00

abstract：:Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus RNA 3. The structure consists of two stem-loops separated by a single-stranded region. RNA 3-derived transcripts were ...

journal_title：Nucleic acids research

authors： Lauber E,Guilley H,Richards K,Jonard G,Gilmer D

更新日期：1997-12-01 00:00:00

abstract：:We recently identified a region of preferential replication initiation, oriGNAI3, near the 3' end of the Chinese hamster GNAI3 gene. oriGNAI3 is co-amplified in mutants selected for AMPD2 amplification, a process generating chromosomal rearrangements. In this report we have taken advantage of cell lines with truncated...

journal_title：Nucleic acids research

authors： Toledo F,Lachagès AM,Mayau V,Debatisse M

更新日期：1999-04-01 00:00:00

abstract：:Increasing numbers of proteins, nucleic acids and other molecular entities have been explored as therapeutic targets, hundreds of which are targets of approved and clinical trial drugs. Knowledge of these targets and corresponding drugs, particularly those in clinical uses and trials, is highly useful for facilitating...

journal_title：Nucleic acids research

authors： Zhu F,Han B,Kumar P,Liu X,Ma X,Wei X,Huang L,Guo Y,Han L,Zheng C,Chen Y

更新日期：2010-01-01 00:00:00

abstract：:RNase J is a conserved ribonuclease that belongs to the β-CASP family of nucleases. It possesses both endo- and exo-ribonuclease activities, which play a key role in pre-rRNA maturation and mRNA decay. Here we report high-resolution crystal structures of Deinococcus radiodurans RNase J complexed with RNA or uridine 5'...

journal_title：Nucleic acids research

authors： Zhao Y,Lu M,Zhang H,Hu J,Zhou C,Xu Q,Ul Hussain Shah AM,Xu H,Wang L,Hua Y

更新日期：2015-06-23 00:00:00

abstract：:Error-free lesion bypass and error-prone lesion bypass are important cellular responses to DNA damage during replication, both of which require a DNA polymerase (Pol). To identify lesion bypass DNA polymerases, we have purified human Polkappa encoded by the DINB1 gene and examined its response to damaged DNA templates...

journal_title：Nucleic acids research

authors： Zhang Y,Yuan F,Wu X,Wang M,Rechkoblit O,Taylor JS,Geacintov NE,Wang Z

更新日期：2000-11-01 00:00:00

abstract：:Transcription of 5S RNA gene in Xenopus oocytes requires a 38 kDa transcription factor TFIIIA, which interacts with the 50 bp internal control region of the gene. We looked for TFIIIA-like polypeptides in the extracts of adult Xenopus tissues on the basis of their antigenic cross-reactivity to anti-TFIIIA antibody. Se...

journal_title：Nucleic acids research

authors： Yasui W,Ryoji M

更新日期：1989-07-25 00:00:00

abstract：:A simple and efficient method is described to introduce structurally pre-determined mutations into recombinant genomes of filamentous phage M13. The method rests on gapped duplex DNA (gdDNA) molecules of the phage M13 genome as the key intermediate. In this gdDNA, the (+) and the (shorter) (-) strand carry different g...

journal_title：Nucleic acids research

authors： Kramer W,Drutsa V,Jansen HW,Kramer B,Pflugfelder M,Fritz HJ

更新日期：1984-12-21 00:00:00

abstract：:Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. The CORUM (http://mips.gsf.de/genre/proj/corum/index.html) database is a collection of experimentally verified mammalian protein complexes. Information is manually derived by critical reading of the scient...

journal_title：Nucleic acids research

authors： Ruepp A,Brauner B,Dunger-Kaltenbach I,Frishman G,Montrone C,Stransky M,Waegele B,Schmidt T,Doudieu ON,Stümpflen V,Mewes HW

更新日期：2008-01-01 00:00:00

abstract：:Analysis of 41 histone homologous clones from an isogenic gene library of Drosophila melanogaster showed that non-histone fragments interrupt the histone repetitive clusters at several sites. Long (L) and short (S) forms of the repeating units are distinguished by the insertion of 240 bp into the spacer between H1 and...

journal_title：Nucleic acids research

authors： Matsuo Y,Yamazaki T

更新日期：1989-01-11 00:00:00

abstract：:Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candid...

journal_title：Nucleic acids research

authors： Haselman T,Camp DG,Fox GE

更新日期：1989-03-25 00:00:00

abstract：:Novel techniques for high-throughput steady-state metabolomic profiling yield information about changes of nearly thousands of metabolites. Such metabolomic profiles, when analyzed together with transcriptional profiles, can reveal novel insights about underlying biological processes. While a number of conceptual appr...

journal_title：Nucleic acids research

authors： Sergushichev AA,Loboda AA,Jha AK,Vincent EE,Driggers EM,Jones RG,Pearce EJ,Artyomov MN

更新日期：2016-07-08 00:00:00

abstract：:Five peaks of DNA glycosylase activity showing a preference for MNNG alkylated DNA have been identified from extracts of adapted M. luteus. They are numerically designated as GI to GV in order of their decreasing molecular weights. The first two of these peaks have been highly purified. GI, is a constitutive heat labi...

journal_title：Nucleic acids research

authors： Riazuddin S,Athar A,Ahmed Z,Lali SM,Sohail A

更新日期：1987-08-25 00:00:00

abstract：:Translesion synthesis (TLS) employs low fidelity polymerases to replicate past damaged DNA in a potentially error-prone process. Regulatory mechanisms that prevent TLS-associated mutagenesis are unknown; however, our recent studies suggest that the PCNA-binding protein Spartan plays a role in suppression of damage-ind...

journal_title：Nucleic acids research

authors： Kim MS,Machida Y,Vashisht AA,Wohlschlegel JA,Pang YP,Machida YJ

更新日期：2013-02-01 00:00:00

abstract：:An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its applicat...

journal_title：Nucleic acids research

authors： Burckhardt J,Telford J,Birnstiel ML

更新日期：1979-07-11 00:00:00

abstract：:Adaptation of eucaryotic cDNA to heterologous expression was studied by mutating the translation initiation (TI) region upstream (mTI) and downstream (MTI) of the start codon. In the mTI subregion the 8 bases flanking the invariant Shine-Dalgarno motif GG-AG were mutagenized exhaustively, while the MTI subregion was s...

journal_title：Nucleic acids research

authors： Bücheler US,Werner D,Schirmer RH

更新日期：1992-06-25 00:00:00

abstract：:RNA sequencing has become an important method to perform hypothesis-free characterization of global gene expression. One of the limitations of RNA sequencing is that most sequence reads represent highly expressed transcripts, whereas low level transcripts are challenging to detect. To combine the benefits of tradition...

journal_title：Nucleic acids research

authors： Halvardson J,Zaghlool A,Feuk L

更新日期：2013-01-07 00:00:00

abstract：:Argonaute (Ago) proteins function in RNA silencing as components of the RNA-induced silencing complex (RISC). In lower organisms, the small interfering RNA and miRNA pathways diverge due in part to sorting mechanisms that direct distinct small RNA (sRNA) duplexes onto specific Ago-RISCs. However, such sorting mechanis...

journal_title：Nucleic acids research

authors： Yamakawa N,Okuyama K,Ogata J,Kanai A,Helwak A,Takamatsu M,Imadome K,Takakura K,Chanda B,Kurosaki N,Yamamoto H,Ando K,Matsui H,Inaba T,Kotani A

更新日期：2014-04-01 00:00:00

abstract：:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...

journal_title：Nucleic acids research

authors： Lu Q,Schierer T,Kang SG,Henderson E

更新日期：1998-04-01 00:00:00

abstract：:For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecu...

journal_title：Nucleic acids research

authors： Behn M,Schuermann M

更新日期：1998-03-01 00:00:00

abstract：:Single-strand specific endoribonuclease YbeY has been shown to play an important role in the processing of the 3' end of the 16S rRNA in Escherichia coli. Lack of YbeY results in the accumulation of the 17S rRNA precursor. In contrast to a previous report, we show that Sinorhizobium meliloti YbeY exhibits endoribonucl...

journal_title：Nucleic acids research

authors： Babu VMP,Sankari S,Budnick JA,Caswell CC,Walker GC

更新日期：2020-01-10 00:00:00

abstract：:The positive role of PARP1 in regulation of various nuclear DNA transactions is well established. Although a mitochondrial localization of PARP1 has been suggested, its role in the maintenance of the mitochondrial DNA is currently unknown. Here we investigated the role of PARP1 in the repair of the mitochondrial DNA i...

journal_title：Nucleic acids research

authors： Szczesny B,Brunyanszki A,Olah G,Mitra S,Szabo C

更新日期：2014-12-01 00:00:00

abstract：:Residual dipolar coupling (RDC) represents one of the most exciting emerging NMR techniques for protein structure studies. However, solving a protein structure using RDC data alone is still a highly challenging problem. We report here a computer program, RDC-PROSPECT, for protein structure prediction based on a struct...

journal_title：Nucleic acids research

authors： Qu Y,Guo JT,Olman V,Xu Y

更新日期：2004-01-26 00:00:00

abstract：:E. coli genes for tRNAasp and tRNAtrp are transcribed by eukaryotic RNA polymerase III upon injection into nuclei of Xenopus oocytes. This indicates that some bacterial tRNA genes contain RNA polymerase III promoter sequences. We discuss evolutionary aspects of this finding. ...

journal_title：Nucleic acids research

authors： Folk WR,Hofstetter H,Birnstiel ML

更新日期：1982-11-25 00:00:00

abstract：:Comprehensive understanding of structure and recognition properties of regulatory nucleic acid elements in real time and atomic level is highly important to devise efficient therapeutic strategies. Here, we report the establishment of an innovative biophysical platform using a dual-app nucleoside analog, which serves ...

journal_title：Nucleic acids research

authors： Nuthanakanti A,Ahmed I,Khatik SY,Saikrishnan K,Srivatsan SG

更新日期：2019-07-09 00:00:00

abstract：:A filter binding assay was developed that allows measurement of specific binding of trp repressor to operator DNA. The most important feature of this procedure is the concentration and type of salt present in the binding buffer. Using this assay the dissociation constant of the repressor-operator complex was determine...

journal_title：Nucleic acids research

authors： Klig LS,Crawford IP,Yanofsky C

更新日期：1987-07-10 00:00:00