The intervening sequence of the ribosomal RNA gene is highly conserved between two Tetrahymena species.

abstract：:Post-transcriptional modifications in archaeal RNA are known to be phylogenetically distinct but relatively little is known of tRNA from the Methanococci, a lineage of methanogenic marine euryarchaea that grow over an unusually broad temperature range. Transfer RNAs from Methanococcus vannielii, Methanococcus maripalu...

journal_title：Nucleic acids research

authors： McCloskey JA,Graham DE,Zhou S,Crain PF,Ibba M,Konisky J,Söll D,Olsen GJ

更新日期：2001-11-15 00:00:00

abstract：:A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two promi...

journal_title：Nucleic acids research

authors： Toloczyki C,Feix G

更新日期：1986-06-25 00:00:00

abstract：:We provide a novel web service, called rQuant.web, allowing convenient access to tools for quantitative analysis of RNA sequencing data. The underlying quantitation technique rQuant is based on quadratic programming and estimates different biases induced by library preparation, sequencing and read mapping. It can tack...

journal_title：Nucleic acids research

authors： Bohnert R,Rätsch G

更新日期：2010-07-01 00:00:00

abstract：:The role of uracil in genomic DNA has been recently re-evaluated. It is now widely accepted to be a physiologically important DNA element in diverse systems from specific phages to antibody maturation and Drosophila development. Further relevant investigations would largely benefit from a novel reliable and fast metho...

journal_title：Nucleic acids research

authors： Róna G,Scheer I,Nagy K,Pálinkás HL,Tihanyi G,Borsos M,Békési A,Vértessy BG

更新日期：2016-02-18 00:00:00

abstract：:The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin gene sequence and from the map previously established by electron microscopy analysis. During evolution of the Y ge...

journal_title：Nucleic acids research

authors： Heilig R,Muraskowsky R,Kloepfer C,Mandel JL

更新日期：1982-07-24 00:00:00

abstract：:Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotid...

journal_title：Nucleic acids research

authors： Gait MJ,Sheppard RC

更新日期：1977-12-01 00:00:00

abstract：:Tandem repeats in DNA have been under intensive study for many years, first, as a consequence of their usefulness as genomic markers and DNA fingerprints and more recently as their role in human disease and regulatory processes has become apparent. The Tandem Repeats Database (TRDB) is a public repository of informati...

journal_title：Nucleic acids research

authors： Gelfand Y,Rodriguez A,Benson G

更新日期：2007-01-01 00:00:00

abstract：:RNA-Seq is gradually becoming the standard tool for transcriptomic expression studies in biological research. Although considerable progress has been recorded in the development of statistical algorithms for the detection of differentially expressed genes using RNA-Seq data, the list of detected genes can differ signi...

journal_title：Nucleic acids research

authors： Moulos P,Hatzis P

更新日期：2015-02-27 00:00:00

abstract：:Non-LTR retrotransposons are mobile genetic elements and play a major role in eukaryotic genome evolution and disease. Similar to retroviruses they encode a reverse transcriptase, but their genomic integration mechanism is fundamentally different, and they lack homologs of the retroviral nucleocapsid-forming protein G...

journal_title：Nucleic acids research

authors： Schneider AM,Schmidt S,Jonas S,Vollmer B,Khazina E,Weichenrieder O

更新日期：2013-12-01 00:00:00

abstract：:The current release of ProTherm, Thermodynamic Database for Proteins and Mutants, contains more than 10 000 numerical data (300% of the first version) of several thermodynamic parameters, experimental methods and conditions, reversibility of folding, details about the surrounding residues in space for all mutants, str...

journal_title：Nucleic acids research

authors： Gromiha MM,Uedaira H,An J,Selvaraj S,Prabakaran P,Sarai A

更新日期：2002-01-01 00:00:00

abstract：:Saccharomyces cerevisiae Pif1 (ScPif1) is known as an ATP-dependent DNA helicase that plays critical roles in a number of important biological processes such as DNA replication, telomere maintenance and genome stability maintenance. Besides its DNA helicase activity, ScPif1 is also known as a single-stranded DNA (ssDN...

journal_title：Nucleic acids research

authors： Lu C,Le S,Chen J,Byrd AK,Rhodes D,Raney KD,Yan J

更新日期：2019-08-22 00:00:00

abstract：:Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-stranded target DNA s...

journal_title：Nucleic acids research

authors： Roulon T,Coulaud D,Delain E,Le Cam E,Hélène C,Escudé C

更新日期：2002-02-01 00:00:00

abstract：:Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5' splice sites (5'ss), we systematically analyzed a set of 76 cryptic 5'ss derived from 46 human genes. These cryptic 5'ss have a similar frequency distribut...

journal_title：Nucleic acids research

authors： Roca X,Sachidanandam R,Krainer AR

更新日期：2003-11-01 00:00:00

abstract：:Many nucleic acid-binding proteins and the AAA+ family form hexameric rings, but the mechanism of hexamer assembly is unclear. It is generally believed that the specificity in protein/RNA interaction relies on molecular contact through a surface charge or 3D structure matching via conformational capture or induced fit...

journal_title：Nucleic acids research

authors： Xiao F,Zhang H,Guo P

更新日期：2008-11-01 00:00:00

abstract：:The EMBL Data Library was the first internationally supported central resource for nucleic acid sequence data. Working in close collaboration with its American counterpart, GenBank (1), the library prepares and makes available to the scientific community a comprehensive collection of the published nucleic acid sequenc...

journal_title：Nucleic acids research

authors： Hamm GH,Cameron GN

更新日期：1986-01-10 00:00:00

abstract：:Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its processivity during plasmid unwinding. Here we show that RepD forms a co...

journal_title：Nucleic acids research

authors： Machón C,Lynch GP,Thomson NH,Scott DJ,Thomas CD,Soultanas P

更新日期：2010-04-01 00:00:00

abstract：:Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an...

journal_title：Nucleic acids research

authors： Herruzo E,Ontoso D,González-Arranz S,Cavero S,Lechuga A,San-Segundo PA

更新日期：2016-09-19 00:00:00

abstract：:Escherichia coli seryl-tRNA synthetase (SerRS) is a homo-dimeric class II aminoacyl-tRNA synthetase. Each subunit is composed of two distinct domains: the N-terminal domain is a 60 A long, arm-like coiled coil structure built up of two antiparallel alpha-helices, whereas the C-terminal domain, the catalytic core, is a...

journal_title：Nucleic acids research

authors： Vincent C,Borel F,Willison JC,Leberman R,Härtlein M

更新日期：1995-04-11 00:00:00

abstract：:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cl...

journal_title：Nucleic acids research

authors： Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv H

更新日期：1981-01-10 00:00:00

abstract：:Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome...

journal_title：Nucleic acids research

authors： Hefel A,Honda M,Cronin N,Harrell K,Patel P,Spies M,Smolikove S

更新日期：2021-01-21 00:00:00

abstract：:The potential use of alpha-beta-anomeric duplex oligonucleotides to inhibit transcription factor activity by the decoy approach is investigated in this report. Indeed, several alpha-beta-anomeric heteroduplexes display a sequence-specific interaction with the p50 subunit of the transcription factor NF kappa B. Used in...

journal_title：Nucleic acids research

authors： Tanaka H,Vickart P,Bertrand JR,Rayner B,Morvan F,Imbach JL,Paulin D,Malvy C

更新日期：1994-08-11 00:00:00

abstract：:The transcriptional activity of a human transposon-like family of repeats, called the THE-1 family, has been studied in cell culture and in human tissue. Both strands of THE-1 are present in several discrete length poly A plus RNAs. Primer extension studies and the structures of cDNA clones show that these THE-1 trans...

journal_title：Nucleic acids research

authors： Paulson KE,Matera AG,Deka N,Schmid CW

更新日期：1987-07-10 00:00:00

abstract：:NRPS-PKS is web-based software for analysing large multi-enzymatic, multi-domain megasynthases that are involved in the biosynthesis of pharmaceutically important natural products such as cyclosporin, rifamycin and erythromycin. NRPS-PKS has been developed based on a comprehensive analysis of the sequence and structur...

journal_title：Nucleic acids research

authors： Ansari MZ,Yadav G,Gokhale RS,Mohanty D

更新日期：2004-07-01 00:00:00

abstract：:The nucleotide sequence of the faeD gene of Escherichia coli and the amino acid sequence of its product is presented. The faeD product is an outer membrane protein required for transport of K88ab fimbrial subunits across the outer membrane. The protein is synthesized as a precursor containing a signal peptide, and the...

journal_title：Nucleic acids research

authors： Mooi FR,Claassen I,Bakker D,Kuipers H,de Graaf FK

更新日期：1986-03-25 00:00:00

abstract：:Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side o...

journal_title：Nucleic acids research

authors： Cochet M,Perrin F,Gannon F,Krust A,Chambon P,McKnight GS,Lee DC,Mayo KE,Palmiter R

更新日期：1979-06-11 00:00:00

abstract：:With the improved rapid sequencing techniques, the earlier sequence of U2 RNA of Novikoff hepatoma (Shibata et al, J. Biol. Chem. 250, 3909-3920, 1975) was reanalyzed and modified. The improved sequence of U2 RNA is 188 (or 189) nucleotides long and is in register with a characterized U2 RNA pseudogene (Denison et al,...

journal_title：Nucleic acids research

authors： Reddy R,Henning D,Epstein P,Busch H

更新日期：1981-11-11 00:00:00

abstract：:This is the first report of a complete mitochondrial genome sequence from a photosynthetic member of the stramenopiles, the chrysophyte alga Chrysodidymus synuroideus. The circular-mapping mitochondrial DNA (mtDNA) of 34 119 bp contains 58 densely packed genes (all without introns) and five unique open reading frames ...

journal_title：Nucleic acids research

authors： Chesnick JM,Goff M,Graham J,Ocampo C,Lang BF,Seif E,Burger G

更新日期：2000-07-01 00:00:00

abstract：:The variation of the proton chemical shifts due to the formation intermolecular hydrogen bonds is computed for a number of complexes which can be formed between the bases of the nucleic acids. The shifts expected for the isolated base pairs, in particular for the G-N1 H, T(or U)-N3H protons and the protons of the amin...

journal_title：Nucleic acids research

authors： Giessner-Prettre C,Pullman B,Caillet J

更新日期：1977-01-01 00:00:00

abstract：:DNA sequencing studies of two recently cloned human A gamma globin alleles has revealed a number of base differences which are clustered in the large intron (IVS-2). One allele has a previously undescribed IVS-2 sequence. Most of the allelic differences can be explained as resulting from a gene conversion event involv...

journal_title：Nucleic acids research

authors： Stoeckert CJ Jr,Collins FS,Weissman SM

更新日期：1984-06-11 00:00:00

abstract：:We have isolated the 5'-end of the gene for the rat and human link protein by screening genomic libraries with oligonucleotides corresponding to the 5'-cDNA sequence. Several overlapping clones were isolated for the human link protein gene, while only one clone was obtained for the rat. All the clones contained a sing...

journal_title：Nucleic acids research

authors： Rhodes C,Savagner P,Line S,Sasaki M,Chirigos M,Doege K,Yamada Y

更新日期：1991-04-25 00:00:00