Structure and properties of the esterase from non-LTR retrotransposons suggest a role for lipids in retrotransposition.

abstract：:Electron microscopic technique was used to examine the structures of SV40 DNA recombination intermediates induced by ultraviolet irradiation as an approach for understanding recombination mechanisms in animal cells. Putative recombination intermediate with the characteristic Holliday junction was observed in both SV40...

journal_title：Nucleic acids research

authors： Hsu MT

更新日期：1991-12-01 00:00:00

abstract：:A very high rate of multidrug resistance (MDR) seen among Gram-negative bacteria such as Escherichia, Klebsiella, Salmonella, Shigella, etc. is a major threat to public health and safety. One of the major virulent determinants of Gram-negative bacteria is capsular polysaccharide or K antigen located on the bacterial o...

journal_title：Nucleic acids research

authors： Kunduru BR,Nair SA,Rathinavelan T

更新日期：2016-01-04 00:00:00

abstract：:Recently, we reported a multicopy mammalian plasmid with a structure related to polyoma. The plasmid, named L factor, was found at a high copy number (5,000 or more per cell) in a subclone derived from mouse L cells. We attempted to utilize L factor as a plasmid vector for mammalian cells. A series of composite DNA co...

journal_title：Nucleic acids research

authors： Saito H,Uehara H,Kusano T,Oishi M

更新日期：1987-11-11 00:00:00

abstract：:GeneAlign is a coding exon prediction tool for predicting protein coding genes by measuring the homologies between a sequence of a genome and related sequences, which have been annotated, of other genomes. Identifying protein coding genes is one of most important tasks in newly sequenced genomes. With increasing numbe...

journal_title：Nucleic acids research

authors： Hsieh SJ,Lin CY,Liu NH,Chow WY,Tang CY

更新日期：2006-07-01 00:00:00

abstract：:MeCP2 is a methyl-CpG binding protein that can repress transcription of nearby genes. In humans, mutations in the MECP2 gene are the major cause of Rett syndrome. By searching expressed sequence tag (EST) databases we have found a novel MeCP2 splice isoform (MeCP2alpha) which encodes a distinct N-terminus. We demonstr...

journal_title：Nucleic acids research

authors： Kriaucionis S,Bird A

更新日期：2004-03-19 00:00:00

abstract：:The self-assembly of bis-biotinylated double-stranded DNA and the tetravalent biotin-binding protein streptavidin (STV) have been studied by non-denaturing gel electrophoresis and atomic force microscopy (AFM). The rapid self-assembly reproducibly generated populations of individual oligomeric complexes. Most striking...

journal_title：Nucleic acids research

authors： Niemeyer CM,Adler M,Pignataro B,Lenhert S,Gao S,Chi L,Fuchs H,Blohm D

更新日期：1999-12-01 00:00:00

abstract：:Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active...

journal_title：Nucleic acids research

authors： Hua J,Garner R,Paetkau V

更新日期：1993-01-11 00:00:00

abstract：:An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional...

journal_title：Nucleic acids research

authors： Hirayama H,Tamaoka J,Horikoshi K

更新日期：1996-10-15 00:00:00

abstract：:Regulatory T-cells (Treg) play an essential role in the negative regulation of immune answers by developing an attenuated cytokine response that allows suppressing proliferation and effector function of T-cells (CD4(+) Th). The transcription factor FoxP3 is responsible for the regulation of many genes involved in the ...

journal_title：Nucleic acids research

authors： Birzele F,Fauti T,Stahl H,Lenter MC,Simon E,Knebel D,Weith A,Hildebrandt T,Mennerich D

更新日期：2011-10-01 00:00:00

abstract：:Stringent starvation protein A (SspA) is an RNA polymerase (RNAP)-associated protein involved in nucleotide metabolism, acid tolerance and virulence of bacteria. Despite extensive biochemical and genetic analyses, the precise regulatory role of SspA in transcription is still unknown, in part, because of a lack of stru...

journal_title：Nucleic acids research

authors： Wang F,Shi J,He D,Tong B,Zhang C,Wen A,Zhang Y,Feng Y,Lin W

更新日期：2020-09-25 00:00:00

abstract：:Allostery is comprehensively studied for natural macromolecules, such as proteins and nucleic acids. Here, we present controllable allostery of synthetic DNA nanostructure-enzyme systems. Rational designs of the synthetic allosteric systems are based on an in-depth understanding of allosteric sites with several types ...

journal_title：Nucleic acids research

authors： Yan Q,Wang Y,Shi J,Wei B

更新日期：2020-07-27 00:00:00

abstract：:The vitamin D receptor mediates the signal of 1 alpha, 25-dihydroxyvitamin D3 by binding to vitamin D responsive elements in DNA as a homodimer or as a heterodimer composed of one vitamin D receptor subunit and one retinoid X receptor subunit. We have mapped the dimerization interfaces of the vitamin D receptor that i...

journal_title：Nucleic acids research

authors： Nishikawa J,Kitaura M,Imagawa M,Nishihara T

更新日期：1995-02-25 00:00:00

abstract：:Here, we describe two freely available web servers for molecular docking. The PatchDock method performs structure prediction of protein-protein and protein-small molecule complexes. The SymmDock method predicts the structure of a homomultimer with cyclic symmetry given the structure of the monomeric unit. The inputs t...

journal_title：Nucleic acids research

authors： Schneidman-Duhovny D,Inbar Y,Nussinov R,Wolfson HJ

更新日期：2005-07-01 00:00:00

abstract：:USF is a helix-loop-helix transcription factor that, like Myc, recognizes the DNA binding motif CACGTG. Two different forms of USF, characterized by apparent molecular weights of 43,000 and 44,000, were originally identified in HeLa cells by biochemical analysis. Clones for the 43-kDa USF were first characterized, but...

journal_title：Nucleic acids research

authors： Sirito M,Lin Q,Maity T,Sawadogo M

更新日期：1994-02-11 00:00:00

abstract：:Exonic splicing enhancer (ESE) sequences are bound by serine & arginine-rich (SR) proteins, which in turn enhance the recruitment of splicing factors. It was inferred from measurements of splicing around twenty years ago that Drosophila doublesex ESEs are bound stably by SR proteins, and that the bound proteins intera...

journal_title：Nucleic acids research

authors： Jobbins AM,Reichenbach LF,Lucas CM,Hudson AJ,Burley GA,Eperon IC

更新日期：2018-03-16 00:00:00

abstract：:We have adapted a method for making libraries of mutations in any specific gene for use in the fission yeast Schizosaccharomyces pombe . This elegant and simple method consists of PCR amplification of the gene of interest, followed by co-transformation of fission yeast with the PCR fragment and a linearized plasmid ve...

journal_title：Nucleic acids research

authors： Kostrub CF,Lei EP,Enoch T

更新日期：1998-10-15 00:00:00

abstract：:Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of...

journal_title：Nucleic acids research

authors： Sansoni V,Casas-Delucchi CS,Rajan M,Schmidt A,Bönisch C,Thomae AW,Staege MS,Hake SB,Cardoso MC,Imhof A

更新日期：2014-06-01 00:00:00

abstract：:Mutations in the gene of human RNase T2 are associated with white matter disease of the human brain. Although brain abnormalities (bilateral temporal lobe cysts and multifocal white matter lesions) and clinical symptoms (psychomotor impairments, spasticity and epilepsy) are well characterized, the pathomechanism of RN...

journal_title：Nucleic acids research

authors： Thorn A,Steinfeld R,Ziegenbein M,Grapp M,Hsiao HH,Urlaub H,Sheldrick GM,Gärtner J,Krätzner R

更新日期：2012-09-01 00:00:00

abstract：:Tcf/Lef family transcription factors are the downstream effectors of the Wingless/Wnt signal transduction pathway. Upon Wingless/Wnt signalling, beta-catenin translocates to the nucleus, interacts with Tcf (1-3) and thus activates transcription of target genes (4,5). Tcf factors also interact with members of the Grouc...

journal_title：Nucleic acids research

authors： Brantjes H,Roose J,van De Wetering M,Clevers H

更新日期：2001-04-01 00:00:00

abstract：:Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-le...

journal_title：Nucleic acids research

authors： Ilyin Y,Chmeliauskaite VG,Kulguskin VV,Georgiev GP

更新日期：1980-11-25 00:00:00

abstract：:EcoGene (http://ecogene.org) is a database and website devoted to continuously improving the structural and functional annotation of Escherichia coli K-12, one of the most well understood model organisms, represented by the MG1655(Seq) genome sequence and annotations. Major improvements to EcoGene in the past decade i...

journal_title：Nucleic acids research

authors： Zhou J,Rudd KE

更新日期：2013-01-01 00:00:00

abstract：:The DNA double helix undergoes an 'overstretching' transition in a narrow force range near 65 pN. Despite numerous studies the basic question of whether the strands are separated or not remains controversial. Here we show that overstretching in fact involves two distinct types of double-helix reorganization: slow hyst...

journal_title：Nucleic acids research

authors： Fu H,Chen H,Marko JF,Yan J

更新日期：2010-09-01 00:00:00

abstract：:DNA from healthy Malaysian newborns was studied on gene maps after digestion with different restriction endonucleases. Of 65 newborns, two were found to be carriers of two different variants of triplicated alpha-globin loci. In variant no. 1, found in an Malay, the three alpha-globin genes are in an elongated DNA frag...

journal_title：Nucleic acids research

authors： Lie-Injo LE,Herrera AR,Kan YW

更新日期：1981-08-11 00:00:00

abstract：:The sigma(54) factor associates with core RNA polymerase (RNAP) to form a holoenzyme that is unable to initiate transcription unless acted on by an activator protein. sigma(54) is closely involved in many steps of activator-dependent transcription, such as core RNAP binding, promoter recognition, activator interaction...

journal_title：Nucleic acids research

authors： Xiao Y,Wigneshweraraj SR,Weinzierl R,Wang YP,Buck M

更新日期：2009-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the DNA damage response (DDR) is activated by the spatio-temporal colocalization of Mec1-Ddc2 kinase and the 9-1-1 clamp. In the absence of direct means to monitor Mec1 kinase activation in vivo, activation of the checkpoint kinase Rad53 has been taken as a proxy for DDR activation. Here, ...

journal_title：Nucleic acids research

authors： Janke R,Herzberg K,Rolfsmeier M,Mar J,Bashkirov VI,Haghnazari E,Cantin G,Yates JR 3rd,Heyer WD

更新日期：2010-04-01 00:00:00

abstract：:Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced sile...

journal_title：Nucleic acids research

authors： Zhang J,Liu H,Yao Q,Yu X,Chen Y,Cui R,Wu B,Zheng L,Zuo J,Huang Z,Ma J,Gan J

更新日期：2016-11-02 00:00:00

abstract：:Yeast tRNA Val 1 alkylation with 2', 3'-O-4-(N-2-chloroethyl-N-methylamino) benzylidene d(pC-G)-A proceeds at 20 degrees - 30 degrees C in the complementary complexes which are formed by d(pC-G)-A greater than RC1 binding to 3 sequences of tRNA Val 1 : psi-C-G58 in the T loop, C-G40 at the 3'-side of the anticodon loo...

journal_title：Nucleic acids research

authors： Grineva NI,Karpova GG,Kuznetsova LM,Venkstern TV,Bayev AA

更新日期：1977-01-01 00:00:00

abstract：:Ribosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional downshift. Here, we show t...

journal_title：Nucleic acids research

authors： Bosio MC,Fermi B,Spagnoli G,Levati E,Rubbi L,Ferrari R,Pellegrini M,Dieci G

更新日期：2017-05-05 00:00:00

abstract：:The ORFDB (http://orf.invitrogen.com/) represents an ongoing effort at Invitrogen Corporation to integrate relevant scientific data with an evolving collection of human and mouse Open Reading Frame (ORF) clones (Ultimate ORF Clones). The ORFDB serves as a central data warehouse enabling researchers to search the ORF c...

journal_title：Nucleic acids research

authors： Liang F,Matrubutham U,Parvizi B,Yen J,Duan D,Mirchandani J,Hashima S,Nguyen U,Ubil E,Loewenheim J,Yu X,Sipes S,Williams W,Wang L,Bennett R,Carrino J

更新日期：2004-01-01 00:00:00

abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...

journal_title：Nucleic acids research

authors： Cupane A,Vitrano E,San Biago PL,Madonia F,Palma MU

更新日期：1980-09-25 00:00:00