Visualization of novel simian virus 40 DNA recombination intermediates induced by ultraviolet light irradiation.

abstract：:Genome-wide association studies often incorporate information from public biological databases in order to provide a biological reference for interpreting the results. The dbSNP database is an extensive source of information on single nucleotide polymorphisms (SNPs) for many different organisms, including humans. We h...

journal_title：Nucleic acids research

authors： Saccone SF,Quan J,Mehta G,Bolze R,Thomas P,Deelman E,Tischfield JA,Rice JP

更新日期：2011-01-01 00:00:00

abstract：:DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In a...

journal_title：Nucleic acids research

authors： Deyle DR,Li LB,Ren G,Russell DW

更新日期：2014-03-01 00:00:00

abstract：:Upon endocytosis, most oligodeoxynucleotides (ODNs) accumulate in vesicular compartments; a tiny number of them cross the vesicle membrane, reach the cytosol and by passive diffusion enter the nucleus where they are entrapped. So far, the compartment in which an antisense ODN interacts with its mRNA target has not bee...

journal_title：Nucleic acids research

authors： Meunier L,Mayer R,Monsigny M,Roche AC

更新日期：1999-07-01 00:00:00

abstract：:The accurate structural modeling of B- and T-cell receptors is fundamental to gain a detailed insight in the mechanisms underlying immunity and in developing new drugs and therapies. The LYRA (LYmphocyte Receptor Automated modeling) web server (http://www.cbs.dtu.dk/services/LYRA/) implements a complete and automated ...

journal_title：Nucleic acids research

authors： Klausen MS,Anderson MV,Jespersen MC,Nielsen M,Marcatili P

更新日期：2015-07-01 00:00:00

abstract：:The nucleotide sequence of the faeD gene of Escherichia coli and the amino acid sequence of its product is presented. The faeD product is an outer membrane protein required for transport of K88ab fimbrial subunits across the outer membrane. The protein is synthesized as a precursor containing a signal peptide, and the...

journal_title：Nucleic acids research

authors： Mooi FR,Claassen I,Bakker D,Kuipers H,de Graaf FK

更新日期：1986-03-25 00:00:00

abstract：:This work presents the Apo-Holo DataBase (AH-DB, http://ahdb.ee.ncku.edu.tw/ and http://ahdb.csbb.ntu.edu.tw/), which provides corresponding pairs of protein structures before and after binding. Conformational transitions are commonly observed in various protein interactions that are involved in important biological f...

journal_title：Nucleic acids research

authors： Chang DT,Yao TJ,Fan CY,Chiang CY,Bai YH

更新日期：2012-01-01 00:00:00

abstract：:The frequencies of each of the 257 468 complete protein coding sequences (CDSs) have been compiled from the taxonomical divisions of the GenBank DNA sequence database. The sum of the codons used by 8792 organisms has also been calculated. The data files can be obtained from the anonymous ftp sites of DDBJ, Kazusa and ...

journal_title：Nucleic acids research

authors： Nakamura Y,Gojobori T,Ikemura T

更新日期：2000-01-01 00:00:00

abstract：:Mitotic chromosome structure is pivotal to cell division but difficult to observe in fine detail using conventional methods. DNA catenation has been implicated in both sister chromatid cohesion and chromosome condensation, but has never been observed directly. We have used a lab-on-a-chip microfluidic device and fluor...

journal_title：Nucleic acids research

authors： Bauer DL,Marie R,Rasmussen KH,Kristensen A,Mir KU

更新日期：2012-12-01 00:00:00

abstract：:High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...

journal_title：Nucleic acids research

authors： Burel SA,Hart CE,Cauntay P,Hsiao J,Machemer T,Katz M,Watt A,Bui HH,Younis H,Sabripour M,Freier SM,Hung G,Dan A,Prakash TP,Seth PP,Swayze EE,Bennett CF,Crooke ST,Henry SP

更新日期：2016-03-18 00:00:00

abstract：:Bacillus subtilis OhrR is a dimeric repressor that senses organic peroxides and regulates the expression of the OhrA peroxiredoxin. Derepression results from oxidation of an active site cysteine which ultimately results in formation of a mixed disulfide with a low molecular weight thiol, a cyclic sulfenamide, or overo...

journal_title：Nucleic acids research

authors： Eiamphungporn W,Soonsanga S,Lee JW,Helmann JD

更新日期：2009-03-01 00:00:00

abstract：:The cnjC gene from the protozoan Tetrahymena thermophila was completely sequenced. The deduced gene product was found to have significant sequence similarity to the yeast and prokaryotic RNA polymerase subunits involved with subunit assembly. Since cnjC is active only during the sexual stage (conjugation) of Tetrahyme...

journal_title：Nucleic acids research

authors： Martindale DW

更新日期：1990-05-25 00:00:00

abstract：:Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1 gene. Mutations in ...

journal_title：Nucleic acids research

authors： Muñoz IG,Prieto J,Subramanian S,Coloma J,Redondo P,Villate M,Merino N,Marenchino M,D'Abramo M,Gervasio FL,Grizot S,Daboussi F,Smith J,Chion-Sotinel I,Pâques F,Duchateau P,Alibés A,Stricher F,Serrano L,Blanco FJ,Mo

更新日期：2011-01-01 00:00:00

abstract：:The genome-wide identification of microRNA transcription start sites (miRNA TSSs) is essential for understanding how miRNAs are regulated in development and disease. In this study, we developed mirSTP (mirna transcription Start sites Tracking Program), a probabilistic model for identifying active miRNA TSSs from nasce...

journal_title：Nucleic acids research

authors： Liu Q,Wang J,Zhao Y,Li CI,Stengel KR,Acharya P,Johnston G,Hiebert SW,Shyr Y

更新日期：2017-07-27 00:00:00

abstract：:Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and cr...

journal_title：Nucleic acids research

authors： Crespo M,Damont A,Blanco M,Lastrucci E,Kennani SE,Ialy-Radio C,Khattabi LE,Terrier S,Louwagie M,Kieffer-Jaquinod S,Hesse AM,Bruley C,Chantalat S,Govin J,Fenaille F,Battail C,Cocquet J,Pflieger D

更新日期：2020-05-07 00:00:00

abstract：:MicroRNAs are a class of small non-coding RNAs that serve as important regulators of gene expression at the posttranscriptional level. They are stable in body fluids and pose great potential to serve as biomarkers. Here, we present a highly specific, sensitive and cost-effective system to quantify miRNA expression bas...

journal_title：Nucleic acids research

authors： Androvic P,Valihrach L,Elling J,Sjoback R,Kubista M

更新日期：2017-09-06 00:00:00

abstract：:The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation bet...

journal_title：Nucleic acids research

authors： Suda T,Mishima Y,Takayanagi K,Asakura H,Odani S,Kominami R

更新日期：1996-12-01 00:00:00

abstract：:The underlying mechanism of transcriptional co-repressor ETO2 during early erythropoiesis and hemoglobin switching is unclear. We find that absence of ETO2 in mice interferes with down-regulation of PU.1 and GATA2 in the fetal liver, impeding a key step required for commitment to erythroid maturation. In human β-globi...

journal_title：Nucleic acids research

authors： Guo X,Plank-Bazinet J,Krivega I,Dale RK,Dean A

更新日期：2020-10-09 00:00:00

abstract：:Chemically modified siRNAs are expected to have resistance toward nuclease degradation and good thermal stability in duplex formation for in vivo applications. We have recently found that 2'-OMe-4'-thioRNA, a hybrid chemical modification based on 2'-OMeRNA and 4'-thioRNA, has high hybridization affinity for complement...

journal_title：Nucleic acids research

authors： Takahashi M,Nagai C,Hatakeyama H,Minakawa N,Harashima H,Matsuda A

更新日期：2012-07-01 00:00:00

abstract：:Ribosomal protein (rp)S5 belongs to the family of the highly conserved rp's that contains rpS7 from prokaryotes and rpS5 from eukaryotes. Alignment of rpS5/rpS7 from metazoans (Homo sapiens), fungi (Saccharomyces cerevisiae) and bacteria (Escherichia coli) shows that the proteins contain a conserved central/C-terminal...

journal_title：Nucleic acids research

authors： Lumsden T,Bentley AA,Beutler W,Ghosh A,Galkin O,Komar AA

更新日期：2010-03-01 00:00:00

abstract：:Ribonucleoprotein (RNP) granules are membraneless organelles (MLOs), which majorly consist of RNA and RNA-binding proteins and are formed via liquid-liquid phase separation (LLPS). Experimental studies investigating the drivers of LLPS have shown that intrinsically disordered proteins (IDPs) and nucleic acids like RNA...

journal_title：Nucleic acids research

authors： Regy RM,Dignon GL,Zheng W,Kim YC,Mittal J

更新日期：2020-12-16 00:00:00

abstract：:A mass spectral method is described for the detection and identification of unusual nucleotide residues present in DNAs. Analysis by this method of intact, underivatized DNA from salmon sperm, calf thymus, mouse L-cells, wheat germ, M. lysodeikticus, E. Coli, and the bacteriophages 0X-174, fd, and lamda, yields diagno...

journal_title：Nucleic acids research

authors： Wiebers JL

更新日期：1976-11-01 00:00:00

abstract：:We have constructed and tested several new vectors for P element-mediated gene transfer. These vectors contain restriction sites for cloning a wide variety of DNA fragments within a small, non-autonomous P element and can be used to efficiently transduce microinjected DNA sequences into the germ line chromosomes of D....

journal_title：Nucleic acids research

authors： Rubin GM,Spradling AC

更新日期：1983-09-24 00:00:00

abstract：:Phage_Finder, a heuristic computer program, was created to identify prophage regions in completed bacterial genomes. Using a test dataset of 42 bacterial genomes whose prophages have been manually identified, Phage_Finder found 91% of the regions, resulting in 7% false positive and 9% false negative prophages. A searc...

journal_title：Nucleic acids research

authors： Fouts DE

更新日期：2006-01-01 00:00:00

abstract：:Five peaks of DNA glycosylase activity showing a preference for MNNG alkylated DNA have been identified from extracts of adapted M. luteus. They are numerically designated as GI to GV in order of their decreasing molecular weights. The first two of these peaks have been highly purified. GI, is a constitutive heat labi...

journal_title：Nucleic acids research

authors： Riazuddin S,Athar A,Ahmed Z,Lali SM,Sohail A

更新日期：1987-08-25 00:00:00

abstract：:Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...

journal_title：Nucleic acids research

authors： Goldman-Levi R,Miller C,Bogoch J,Zak NB

更新日期：1996-08-15 00:00:00

abstract：:Full-length cDNA of the 6.3 kb turnip yellow mosaic virus (TYMV) genome was placed between a T7 promoter and a unique Hind III site. In vitro transcription of Hind III-linearized DNA of clone pTYMC yielded full-length RNA transcripts. In inoculations of Chinese cabbage protoplasts and plants, capped transcripts and vi...

journal_title：Nucleic acids research

authors： Weiland JJ,Dreher TW

更新日期：1989-06-26 00:00:00

abstract：:The centromere is a highly specialized chromosomal element that is essential for chromosome segregation during mitosis. Centromere integrity must therefore be properly preserved and is strictly dependent upon the establishment and maintenance of surrounding chromatin structure. Here we identify WDHD1, a WD40-domain an...

journal_title：Nucleic acids research

authors： Hsieh CL,Lin CL,Liu H,Chang YJ,Shih CJ,Zhong CZ,Lee SC,Tan BC

更新日期：2011-05-01 00:00:00

abstract：:The Prp19-associated complex is required for spliceosome activation by stabilizing the binding of U5 and U6 on the spliceosome after the release of U4. The complex comprises at least eight proteins, among which Ntc90 and Ntc77 contain multiple tetratricopeptide repeat (TPR) elements. We have previously shown that Ntc9...

journal_title：Nucleic acids research

authors： Chen HC,Chang KJ,Su YL,Huang YH,Cheng SC

更新日期：2014-10-29 00:00:00

abstract：:The restriction endonuclease (R.BsuFI) of Bacillus subtilis recognizes the target DNA sequence 5' CCGG. The R.BsuFI gene was found in close proximity to the cognate M.BsuFI gene, which had previously been characterized (1). Cloning of the R.BsuFI gene in E.coli was only possible with the M.BsuFI Mtase gene present on ...

journal_title：Nucleic acids research

authors： Kapfer W,Walter J,Trautner TA

更新日期：1991-12-11 00:00:00

abstract：:The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonst...

journal_title：Nucleic acids research

authors： Brou C,Wu J,Ali S,Scheer E,Lang C,Davidson I,Chambon P,Tora L

更新日期：1993-01-11 00:00:00