Yeast strains with N-terminally truncated ribosomal protein S5: implications for the evolution, structure and function of the Rps5/Rps7 proteins.

abstract：:The role of thymine dimerization for previously reported U.V. induced conformational changes has been proved using acetophenone as sensitizer for the specific thymine dimerization upon irradiation at 313 nm. CD results demonstrate that formation of pyrimidine dimers cause typical conformational changes of the DNA B he...

journal_title：Nucleic acids research

authors： Lang H

更新日期：1975-02-01 00:00:00

abstract：:Protein-RNA interactions in small nuclear ribonucleoproteins (UsnRNPs) from HeLa cells were investigated by irradiation of purified nucleoplasmic snRNPs U1 to U6 with UV light at 254 nm. The cross-linked proteins were analyzed on one- and two-dimensional gel electrophoresis systems, and the existence of a stable cross...

journal_title：Nucleic acids research

authors： Woppmann A,Rinke J,Lührmann R

更新日期：1988-12-09 00:00:00

abstract：:Targeted differentiation of human induced pluripotent stem cells (hiPSCs) using only chemicals would have value-added clinical potential in the regeneration of complex cell types including cardiomyocytes. Despite the availability of several chemical inhibitors targeting proteins involved in signaling pathways, no bioa...

journal_title：Nucleic acids research

authors： Taniguchi J,Pandian GN,Hidaka T,Hashiya K,Bando T,Kim KK,Sugiyama H

更新日期：2017-09-19 00:00:00

abstract：:The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5' terminal) was found to be common to both members. The Alu family sequence was found in the 5' flanking regions. The KpnI ...

journal_title：Nucleic acids research

authors： Miyake T,Migita K,Sakaki Y

更新日期：1983-10-11 00:00:00

abstract：:The transcriptional activity of a human transposon-like family of repeats, called the THE-1 family, has been studied in cell culture and in human tissue. Both strands of THE-1 are present in several discrete length poly A plus RNAs. Primer extension studies and the structures of cDNA clones show that these THE-1 trans...

journal_title：Nucleic acids research

authors： Paulson KE,Matera AG,Deka N,Schmid CW

更新日期：1987-07-10 00:00:00

abstract：:The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs...

journal_title：Nucleic acids research

authors： Araco A,Belli M,Giorgi C,Onori G

更新日期：1975-03-01 00:00:00

abstract：:A correlation between histone acetylation and transcription has been noted for a long time, but little is known about what step(s) in the transcription cycle is influenced by acetylation. We have examined the immediate transcriptional response to histone deacetylase (HDAC) inhibition, and find that release of promoter...

journal_title：Nucleic acids research

authors： Vaid R,Wen J,Mannervik M

更新日期：2020-05-21 00:00:00

abstract：:Intragenomic and intergenomic comparisons of upstream nucleotide sequences of archaeal genes were performed with the goal of predicting transcription regulatory sites (operators) and identifying likely regulons. Learning sets for the detection of regulatory sites were constructed using the available experimental data ...

journal_title：Nucleic acids research

authors： Gelfand MS,Koonin EV,Mironov AA

更新日期：2000-02-01 00:00:00

abstract：:Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...

journal_title：Nucleic acids research

authors： Serçinoglu O,Ozbek P

更新日期：2018-07-02 00:00:00

abstract：:The transcription regulator, tvMyb1, is the first Myb family protein identified in Trichomonas vaginalis. Using an electrophoretic mobility shift assay, we defined the amino-acid sequence from Lys(35) to Ser(141) (tvMyb1(35-141)) as the minimal DNA-binding domain, encompassing two Myb-like DNA-binding motifs (designat...

journal_title：Nucleic acids research

authors： Lou YC,Wei SY,Rajasekaran M,Chou CC,Hsu HM,Tai JH,Chen C

更新日期：2009-04-01 00:00:00

abstract：:Deep mutational scanning has been used to create high-resolution DNA sequence maps that illustrate the functional consequences of large numbers of point mutations. However, this approach has not yet been applied to libraries of genes created by random circular permutation, an engineering strategy that is used to creat...

journal_title：Nucleic acids research

authors： Atkinson JT,Jones AM,Zhou Q,Silberg JJ

更新日期：2018-07-27 00:00:00

abstract：:Understanding the normal state of human tissue transcriptome profiles is essential for recognizing tissue disease states and identifying disease markers. Recently, the Human Protein Atlas and the FANTOM5 consortium have each published extensive transcriptome data for human samples using Illumina-sequenced RNA-Seq and ...

journal_title：Nucleic acids research

authors： Yu NY,Hallström BM,Fagerberg L,Ponten F,Kawaji H,Carninci P,Forrest AR,Fantom Consortium.,Hayashizaki Y,Uhlén M,Daub CO

更新日期：2015-08-18 00:00:00

abstract：:The location of proteins on the mitochondrial DNA (mtDNA) of Drosophila virilis was investigated by Me3 psoralen photoreaction of mitochondria isolated from embryos. After photoreaction the mtDNA was purified and the pattern of DNA cross-linking was determined by electron microscopy of the DNA under totally denaturing...

journal_title：Nucleic acids research

authors： Pardue ML,Fostel JM,Cech TR

更新日期：1984-02-24 00:00:00

abstract：:In vitro repair of single strand breaks in T4 and phage DNA caused by 32p decay was studied. Zone centrifugation procedure showed that polynucleotide kinase, ligase enzyme system failed to repair 32P-damages. It was found that damaged DNA contained gaps and could be repaired by DNA-polymerase I, polynucleotide ligase ...

journal_title：Nucleic acids research

authors： Fodor I,Matvienko NI,Zlotnikov KM,Tanyashin VI,Solonin AS,Bayev AA

更新日期：1975-05-01 00:00:00

abstract：:Advances in genomic sequencing have enormous potential to revolutionize personalized medicine, however distinguishing disease-causing from benign variants remains a challenge. The increasing number of human genome and exome sequences available has revealed areas where unfavourable variation is removed through purifyin...

journal_title：Nucleic acids research

authors： Silk M,Petrovski S,Ascher DB

更新日期：2019-07-02 00:00:00

abstract：:Thermal elution poly(U)-Sepharose chromatography was utilized to fractionate yeast mRNA based on poly(A) size. Analysis of the in vitro translation products of the fractionated RNAs in a wheat-embryo cell-free protein synthesis system shows a heterogeneous but equal distribution of these abundant translatable mRNAs in...

journal_title：Nucleic acids research

authors： Saunders CA,Bostian KA,Halvorson HO

更新日期：1980-09-11 00:00:00

abstract：:The annotation of protein function has become a crucial problem with the advent of sequence and structural genomics initiatives. A large body of evidence suggests that protein structural information is frequently encoded in local sequences, and that folds are mainly made up of a number of simple local units of super-s...

journal_title：Nucleic acids research

authors： Espadaler J,Fernandez-Fuentes N,Hermoso A,Querol E,Aviles FX,Sternberg MJ,Oliva B

更新日期：2004-01-01 00:00:00

abstract：:Although the thermophilic bacterium Thermus aquaticus grows optimally at 70 degrees C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degrees C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-terminal ...

journal_title：Nucleic acids research

authors： Kermekchiev MB,Tzekov A,Barnes WM

更新日期：2003-11-01 00:00:00

abstract：:Gene II protein is the only phage-encoded protein required for the double-stranded DNA replication of the distantly related filamentous phages IKe and Ff (M13, fd and f1). Complementation studies have demonstrated that, despite a significant degree of homology between the nucleotide sequences of the gene II's of IKe a...

journal_title：Nucleic acids research

authors： Peeters BP,Schoenmakers JG,Konings RN

更新日期：1986-06-25 00:00:00

abstract：:Hepatitis C virus (HCV) infection is a major cause of human chronic liver disease and hepatocellular carcinoma. G-quadruplex (G4) is an important four-stranded secondary structure of nucleic acids. Recently, we discovered that the core gene of HCV contains a G4 RNA structure; however, the interaction between the HCV c...

journal_title：Nucleic acids research

authors： Bian WX,Xie Y,Wang XN,Xu GH,Fu BS,Li S,Long G,Zhou X,Zhang XL

更新日期：2019-01-10 00:00:00

abstract：:A limited number of Methicillin-resistant Staphylococcus aureus (MRSA) clones are responsible for MRSA infections worldwide, and those of different lineages carry unique Type I restriction-modification (RM) variants. We have identified the specific DNA sequence targets for the dominant MRSA lineages CC1, CC5, CC8 and ...

journal_title：Nucleic acids research

authors： Roberts GA,Houston PJ,White JH,Chen K,Stephanou AS,Cooper LP,Dryden DT,Lindsay JA

更新日期：2013-08-01 00:00:00

abstract：:In continuation with work regarding the evaluation of the energy of association of various RNA bases with various base pairs, the results of the computations of the electrostatic interaction energy of A-U base pair with four RNA bases viz. Adenine, Uracil, Guanine and Cytosine have been reported. Non bonded induced po...

journal_title：Nucleic acids research

authors： Sanyal NK,Kumar U,Roychoudhury M

更新日期：1980-09-11 00:00:00

abstract：:The genome of Kirsten murine sarcoma virus was formed by recombination between Kirsten murine leukaemia virus sequences, and rat sequences derived from a retrovirus-like '30S' (VL30) genetic element encompassing the Kras oncogene. Using cloned DNAs we have determined the nucleotide sequences of the long terminal repea...

journal_title：Nucleic acids research

authors： Norton JD,Connor J,Avery RJ

更新日期：1984-09-11 00:00:00

abstract：:Deamination of cytosine (C), 5-methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) occurs spontaneously in mammalian DNA with several hundred deaminations occurring in each cell every day. The resulting potentially mutagenic mispairs of uracil (U), thymine (T) or 5-hydroxymethyluracil (hmU) with guanine (G) are subs...

journal_title：Nucleic acids research

authors： Kemmerich K,Dingler FA,Rada C,Neuberger MS

更新日期：2012-07-01 00:00:00

abstract：:The speed of the new DNA sequencing techniques has created a need for computer programs to handle the data produced. This paper describes simple programs designed specifically for use by people with little or no computer experience. The programs are for use on small computers and provide facilities for storage, editin...

journal_title：Nucleic acids research

authors： Staden R

更新日期：1977-11-01 00:00:00

abstract：:We present the results of in vitro binding studies aimed at defining the key recognition elements on the MS2 RNA translational operator (TR) essential for complex formation with coat protein. We have used chemically synthesized operators carrying modified functional groups at defined nucleotide positions, which are es...

journal_title：Nucleic acids research

authors： Stockley PG,Stonehouse NJ,Murray JB,Goodman ST,Talbot SJ,Adams CJ,Liljas L,Valegård K

更新日期：1995-07-11 00:00:00

abstract：:The E. coli single-stranded binding protein (SSB) has been demonstrated in vitro to be involved in a number of replicative, DNA renaturation, and protective functions. It was shown previously that SSB can interact with exonuclease I to stimulate the hydrolysis of single-stranded DNA. We demonstrate here that E. coli S...

journal_title：Nucleic acids research

authors： Sandigursky M,Franklin WA

更新日期：1994-01-25 00:00:00

abstract：:The EcoCyc database describes the genome and gene products of Escherichia coli, its metabolic and signal-transduction pathways, and its tRNAs. The database describes 4391 genes of E.coli, 695 enzymes encoded by a subset of these genes, 904 metabolic reactions that occur in E.coli, and the organization of these reactio...

journal_title：Nucleic acids research

authors： Karp PD,Riley M,Paley SM,Pellegrini-Toole A,Krummenacker M

更新日期：1999-01-01 00:00:00

abstract：:Myotonic dystrophy type 1 (DM1) is caused by the expansion of a (CTG).(CAG) repeat in the DMPK gene on chromosome 19q13.3. At least 17 neurological diseases have similar genetic mutations, the expansion of DNA repeats. In most of these disorders, the disease severity is related to the length of the repeat expansion, a...

journal_title：Nucleic acids research

authors： Hashem VI,Pytlos MJ,Klysik EA,Tsuji K,Khajavi M,Ashizawa T,Sinden RR

更新日期：2004-12-01 00:00:00

abstract：:We investigated how Escherichia coli ClpXP targets the helicase-nuclease (HsdR) subunit of the bacterial Type I restriction-modification enzyme EcoKI during restriction alleviation (RA). RA is a temporary reduction in endonuclease activity that occurs when Type I enzymes bind unmodified recognition sites on the host g...

journal_title：Nucleic acids research

authors： Simons M,Diffin FM,Szczelkun MD

更新日期：2014-10-29 00:00:00