DNA-protein interactions in the Drosophila virilis mitochondrial chromosome.

abstract：:The third intron from Physarum polycephalum (Pp LSU 3) is one of the closest known relatives to the well-studied Tetrahymena group I intron. Both introns are located at the same position in the 26S rRNA gene, and with the exception of an open reading frame in Pp LSU 3, are highly homologous. While Pp LSU 3 has been sh...

journal_title：Nucleic acids research

authors： Rocheleau GA,Woodson SA

更新日期：1994-10-11 00:00:00

abstract：:Codon usage data has been compiled for 110 yeast genes. Cluster analysis on relative synonymous codon usage revealed two distinct groups of genes. One group corresponds to highly expressed genes, and has much more extreme synonymous codon preference. The pattern of codon usage observed is consistent with that expected...

journal_title：Nucleic acids research

authors： Sharp PM,Tuohy TM,Mosurski KR

更新日期：1986-07-11 00:00:00

abstract：:Search of interrelationships between the structural-functional protein organization and exon structure of encoding gene provides insights into issues concerned with the function, origin and evolution of genes and proteins. The functions of proteins and their domains are defined mostly by functional sites. The relation...

journal_title：Nucleic acids research

authors： Medvedeva I,Demenkov P,Kolchanov N,Ivanisenko V

更新日期：2012-01-01 00:00:00

abstract：:Phosphorylation of the easily accessible 3',5'-diesters 1a-d with diphenyl phosphorochloridate, followed by selective 5'-deacylation, affords the phosphotriester derivatives 2a-d in good yields. Alkaline treatment of 2a-d results in the formation of the 2',3'-cyclic phosphates (3a-d). The usefulness of the phosphotrie...

journal_title：Nucleic acids research

authors： van Boom JH,Burgers PM,Haasnoot CA

更新日期：1976-10-01 00:00:00

abstract：:A study of the activity of deoxyribonucleotide-substituted analogs of the hammerhead domain of RNA catalysis has led to the design of a 14mer oligomer composed entirely of deoxyribonucleotides that promotes the cleavage of an RNA substrate. Characterization of this reaction with sequence variants and mixed DNA/RNA oli...

journal_title：Nucleic acids research

authors： Chartrand P,Harvey SC,Ferbeyre G,Usman N,Cedergren R

更新日期：1995-10-25 00:00:00

abstract：:The comprehensive multiplatform genomics data generated by The Cancer Genome Atlas (TCGA) Research Network is an enabling resource for cancer research. It includes an unprecedented amount of microRNA sequence data: ~11 000 libraries across 33 cancer types. Combined with initiatives like the National Cancer Institute G...

journal_title：Nucleic acids research

authors： Chu A,Robertson G,Brooks D,Mungall AJ,Birol I,Coope R,Ma Y,Jones S,Marra MA

更新日期：2016-01-08 00:00:00

abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...

journal_title：Nucleic acids research

authors： Cupane A,Vitrano E,San Biago PL,Madonia F,Palma MU

更新日期：1980-09-25 00:00:00

abstract：:Key components of the translational apparatus, i.e. ribosomes, elongation factor EF-Tu and most aminoacyl-tRNA synthetases, are stereoselective and prevent incorporation of d-amino acids (d-aa) into polypeptides. The rare appearance of d-aa in natural polypeptides arises from post-translational modifications or non-ri...

journal_title：Nucleic acids research

authors： Achenbach J,Jahnz M,Bethge L,Paal K,Jung M,Schuster M,Albrecht R,Jarosch F,Nierhaus KH,Klussmann S

更新日期：2015-07-13 00:00:00

abstract：:Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...

journal_title：Nucleic acids research

authors： Tsai-Morris CH,Cao XM,Sukhatme VP

更新日期：1988-09-26 00:00:00

abstract：:Melibiose uptake and hydrolysis in E.coli is performed by the MelB and MelA proteins, respectively. We report the cloning and sequencing of the melA gene. The nucleotide sequence data showed that melA codes for a 450 amino acid long protein with a molecular weight of 50.6 kd. The sequence data also supported the assum...

journal_title：Nucleic acids research

authors： Liljeström PL,Liljeström P

更新日期：1987-03-11 00:00:00

abstract：:We report the use of MonoQ FPLC (Fast Protein Liquid Chromatography) for the rapid purification of a novel Type II restriction endonuclease PmaCI, from Pseudomonas maltophila, which recognises the sequence 5'-CAC decreases GTG-3'. The resulting enzyme is free of other nucleases to a level suitable for its characterisa...

journal_title：Nucleic acids research

authors： Walker JN,Dean PD,Saunders JR

更新日期：1986-02-11 00:00:00

abstract：:We have developed a comprehensive resource devoted to biologists wanting to optimize the use of gene trap clones in their experiments. We have processed 300 602 such clones from both public and private projects to generate 28,199 'UniTraps', i.e. distinct collections of unambiguous insertions at the same subgenic regi...

journal_title：Nucleic acids research

authors： Roma G,Sardiello M,Cobellis G,Cruz P,Lago G,Sanges R,Stupka E

更新日期：2008-01-01 00:00:00

abstract：:Pyrrole-imidazole polyamides targeted to the androgen response element were cytotoxic in multiple cell lines, independent of intact androgen receptor signaling. Polyamide treatment induced accumulation of S-phase cells and of PCNA replication/repair foci. Activation of a cell cycle checkpoint response was evidenced by...

journal_title：Nucleic acids research

authors： Martínez TF,Phillips JW,Karanja KK,Polaczek P,Wang CM,Li BC,Campbell JL,Dervan PB

更新日期：2014-10-01 00:00:00

abstract：:Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specifi...

journal_title：Nucleic acids research

authors： Putnam WC,Daniher AT,Trawick BN,Bashkin JK

更新日期：2001-05-15 00:00:00

abstract：:Previously, we developed jumping profile hidden Markov model (jpHMM), a new method to detect recombinations in HIV-1 genomes. The jpHMM predicts recombination breakpoints in a query sequence and assigns to each position of the sequence one of the major HIV-1 subtypes. Since incorrect subtype assignment or recombinatio...

journal_title：Nucleic acids research

authors： Schultz AK,Zhang M,Bulla I,Leitner T,Korber B,Morgenstern B,Stanke M

更新日期：2009-07-01 00:00:00

abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...

journal_title：Nucleic acids research

authors： Pasternak A,Hernandez FJ,Rasmussen LM,Vester B,Wengel J

更新日期：2011-02-01 00:00:00

abstract：:The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and tw...

journal_title：Nucleic acids research

authors： Rotheneder H,Grabner M,Wintersberger E

更新日期：1991-12-25 00:00:00

abstract：:NF-IL6beta regulates gene expression and plays function roles in many tissues. The EGF-regulated cyclooxygenase-2 (cox-2) expression is mediated through p38(MAPK) signaling pathway and positively correlates with NF-IL6beta expression in A431 cells. NF-IL6beta coordinated with c-Jun on cox-2 transcriptional activation ...

journal_title：Nucleic acids research

authors： Wang JM,Ko CY,Chen LC,Wang WL,Chang WC

更新日期：2006-01-05 00:00:00

abstract：:The human embryonal carcinoma cell line NEC14 can be induced to differentiate by the addition of 10(-2)M N,N'-hexamethylene-bis-acetamide (HMBA). A subtractive cDNA library specific to undifferentiated NEC14 cells was constructed using oligo(dT)30-Latex and polymerase chain reaction (PCR). The method was designed to i...

journal_title：Nucleic acids research

authors： Hara E,Kato T,Nakada S,Sekiya S,Oda K

更新日期：1991-12-01 00:00:00

abstract：:The solution structure of the complex between 4', 6-diamidino-2-phenylindole (DAPI) and DNA oligomer [d(GCGATTCGC)]2, containing a central T.T mismatch, has been characterized by combined use of proton one- and two-dimensional NMR spectroscopy, molecular mechanics and molecular dynamics computations including relaxati...

journal_title：Nucleic acids research

authors： Trotta E,Paci M

更新日期：1998-10-15 00:00:00

abstract：:T1DBase (http://www.t1dbase.org) is web platform, which supports the type 1 diabetes (T1D) community. It integrates genetic, genomic and expression data relevant to T1D research across mouse, rat and human and presents this to the user as a set of web pages and tools. This update describes the incorporation of new dat...

journal_title：Nucleic acids research

authors： Burren OS,Adlem EC,Achuthan P,Christensen M,Coulson RM,Todd JA

更新日期：2011-01-01 00:00:00

abstract：:A microchip method has been developed for massive and parallel thermodynamic analyses of DNA duplexes. Fluorescently labeled oligonucleotides were hybridized with oligonucleotides immobilized in the 100 x 100 x 20 mum gel pads of the microchips. The equilibrium melting curves for all microchip duplexes were measured i...

journal_title：Nucleic acids research

authors： Fotin AV,Drobyshev AL,Proudnikov DY,Perov AN,Mirzabekov AD

更新日期：1998-03-15 00:00:00

abstract：:We have purified to near homogeneity a novel nuclear protein from HeLa cells, that specifically binds to scaffold or matrix attachment region DNA elements (S/MAR DNA). The protein, designated SAF-B for scaffold attachment factor B, is an abundant component of chromatin, but not of the nuclear matrix and is expressed i...

journal_title：Nucleic acids research

authors： Renz A,Fackelmayer FO

更新日期：1996-03-01 00:00:00

abstract：:Copy number variants (CNVs) are currently defined as genomic sequences that are polymorphic in copy number and range in length from 1000 to several million base pairs. Among current array-based CNV detection platforms, long-oligonucleotide arrays promise the highest resolution. However, the performance of currently av...

journal_title：Nucleic acids research

authors： Cahan P,Godfrey LE,Eis PS,Richmond TA,Selzer RR,Brent M,McLeod HL,Ley TJ,Graubert TA

更新日期：2008-04-01 00:00:00

abstract：:Homologous recombination (HR) is important in repairing errors of replication and other forms of DNA damage. In mammalian cells, potential templates include the homologous chromosome, and after DNA replication, the sister chromatid. Previous work has shown that the mammalian recombination machinery is organized to sup...

journal_title：Nucleic acids research

authors： Read LR,Raynard SJ,Rukść A,Baker MD

更新日期：2004-02-20 00:00:00

abstract：:The GeneSpeed database (http://genespeed.uchsc.edu/) is an online database and resource tool facilitating the detailed study of protein domain homology in the transcriptomes of Homo sapiens, Mus musculus, Drosophila melanogaster and Caenorhabditis elegans. The population schema for the GeneSpeed database takes advanta...

journal_title：Nucleic acids research

authors： Kutchma A,Quayum N,Jensen J

更新日期：2007-01-01 00:00:00

abstract：:Promoter design remains one of the most important considerations in metabolic engineering and synthetic biology applications. Theoretically, there are 450 possible sequences for a 50-nt promoter, of which naturally occurring promoters make up only a small subset. To explore the vast number of potential sequences, we r...

journal_title：Nucleic acids research

authors： Wang Y,Wang H,Wei L,Li S,Liu L,Wang X

更新日期：2020-07-09 00:00:00

abstract：:Transcript of the rbpA1 gene in Anabaena variabilis accumulates significantly at low growth temperatures below 28 degreesC. This accumulation was maximal at 16 degreesC. Accumulation of the rbpA1 transcript was completely abolished by rifampicin, but not by chloramphenicol. Photosynthesis was not required for this col...

journal_title：Nucleic acids research

authors： Sato N,Nakamura A

更新日期：1998-05-01 00:00:00

abstract：:PvuRts1I is a prototype for a larger family of restriction endonucleases that cleave DNA containing 5-hydroxymethylcytosine (5hmC) or 5-glucosylhydroxymethylcytosine (5ghmC), but not 5-methylcytosine (5mC) or cytosine. Here, we report a crystal structure of the enzyme at 2.35 Å resolution. Although the protein has bee...

journal_title：Nucleic acids research

authors： Kazrani AA,Kowalska M,Czapinska H,Bochtler M

更新日期：2014-05-01 00:00:00

abstract：:The Gene Expression Atlas (http://www.ebi.ac.uk/gxa) is an added-value database providing information about gene expression in different cell types, organism parts, developmental stages, disease states, sample treatments and other biological/experimental conditions. The content of this database derives from curation, ...

journal_title：Nucleic acids research

authors： Kapushesky M,Emam I,Holloway E,Kurnosov P,Zorin A,Malone J,Rustici G,Williams E,Parkinson H,Brazma A

更新日期：2010-01-01 00:00:00