Abstract:
:Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specific RNA cleavage when compared with analogous o-phenanthroline- and terpyridine-derived reagents. The suppression of hydroxide-bridged dimers and the greater activation of coordinated water by Cu(II) neocuproine (compared with the o-phenanthroline and terpyridine complexes) better allow Cu(II) to reach its catalytic potential as a biomimetic RNA cleavage agent. This work demonstrates the direct mapping of molecular design principles from small-molecule cleavage to macromolecular cleavage events, generating enhanced biomimetic, sequence-specific RNA cleavage agents.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Putnam WC,Daniher AT,Trawick BN,Bashkin JKdoi
10.1093/nar/29.10.2199keywords:
subject
Has Abstractpub_date
2001-05-15 00:00:00pages
2199-204issue
10eissn
0305-1048issn
1362-4962journal_volume
29pub_type
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