Abstract:
:Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and consists of 2 exons and one 700 bp intron. S1 nuclease protection and primer extension analysis were used to define the transcription initiation site. "TATA" and "CCAAT" sequences were located at nucleotides -26 and -337 respectively. In addition, there exist five elements whose sequence is nearly identical to the inner core 10 nucleotide region (CCATATTAGG) of the c-fos serum response element, four Sp1 consensus sequences, two AP1 target sequence analogs, and two potential cAMP response elements. These results will ultimately lead to a detailed definition of the intracellular events regulating Egr-1 expression.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tsai-Morris CH,Cao XM,Sukhatme VPdoi
10.1093/nar/16.18.8835subject
Has Abstractpub_date
1988-09-26 00:00:00pages
8835-46issue
18eissn
0305-1048issn
1362-4962journal_volume
16pub_type
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