Purification and molecular cloning of the scaffold attachment factor B (SAF-B), a novel human nuclear protein that specifically binds to S/MAR-DNA.

abstract：:Allostery is the most direct, rapid and efficient way of regulating protein function, ranging from the control of metabolic mechanisms to signal-transduction pathways. However, an enormous amount of unsystematic allostery information has deterred scientists who could benefit from this field. Here, we present the AlloS...

journal_title：Nucleic acids research

authors： Huang Z,Zhu L,Cao Y,Wu G,Liu X,Chen Y,Wang Q,Shi T,Zhao Y,Wang Y,Li W,Li Y,Chen H,Chen G,Zhang J

更新日期：2011-01-01 00:00:00

abstract：:The surface accessibility of macromolecules plays a key role in modulating molecular recognition events. RNA is a complex and dynamic molecule involved in many aspects of gene expression. However, there are few experimental methods available to measure the accessible surface of RNA. Here, we investigate the accessible...

journal_title：Nucleic acids research

authors： Venditti V,Niccolai N,Butcher SE

更新日期：2008-03-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that mediate gene expression at the post-transcriptional and translational levels by an imperfect binding to target mRNA 3'UTR regions. While the ab-initio computational prediction of miRNA-mRNA interactions still poses significant challenges, it is possible to overcome som...

journal_title：Nucleic acids research

authors： Sales G,Coppe A,Bicciato S,Bortoluzzi S,Romualdi C

更新日期：2010-04-01 00:00:00

abstract：:Intragenomic and intergenomic comparisons of upstream nucleotide sequences of archaeal genes were performed with the goal of predicting transcription regulatory sites (operators) and identifying likely regulons. Learning sets for the detection of regulatory sites were constructed using the available experimental data ...

journal_title：Nucleic acids research

authors： Gelfand MS,Koonin EV,Mironov AA

更新日期：2000-02-01 00:00:00

abstract：:A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described. The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins. ...

journal_title：Nucleic acids research

authors： von Heijne G

更新日期：1986-06-11 00:00:00

abstract：:The photostability and narrow emission spectra of non-organic quantum dot fluorophores (QDs) make them desirable candidates for fluorescent in situ hybridization (FISH) to study the expression of specific mRNA transcripts. We developed a novel method for direct QD labeling of modified oligonucleotide probes through st...

journal_title：Nucleic acids research

authors： Chan P,Yuen T,Ruf F,Gonzalez-Maeso J,Sealfon SC

更新日期：2005-10-13 00:00:00

abstract：:Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...

journal_title：Nucleic acids research

authors： Trilling M,Bellora N,Rutkowski AJ,de Graaf M,Dickinson P,Robertson K,Prazeres da Costa O,Ghazal P,Friedel CC,Albà MM,Dölken L

更新日期：2013-09-01 00:00:00

abstract：:MobA protein, encoded by the broad host-range plasmid R1162, is required for conjugal mobilization of this plasmid. The protein is an essential part of the relaxosome, and is also necessary for the termination of strand transfer. In vitro, MobA is a nuclease specific for one of the two DNA strands of the origin of tra...

journal_title：Nucleic acids research

authors： Bhattacharjee MK,Meyer RJ

更新日期：1993-09-25 00:00:00

abstract：:We have studied the structure of recombinant rat UBF (rrUBF), an RNA polymerase I transcription factor, by electron microscopy and image analysis of single particles contrasted with methylamine tungstate. Recombinant rat UBF appeared to be a flat, U-shaped protein with a central region of low density. In the dominant ...

journal_title：Nucleic acids research

authors： Neil KJ,Ridsdale RA,Rutherford B,Taylor L,Larson DE,Glibetic M,Rothblum LI,Harauz G

更新日期：1996-04-15 00:00:00

abstract：:We present a study of how substituent groups of naturally occurring and modified nucleotide bases affect the degree of hydration of right-handed B-DNA and left-handed Z-DNA. A comparison of poly(dG-dC) and poly(dG-dm5C) titrations with the lipotropic salts of the Hofmeister series infers that the methyl stabilization ...

journal_title：Nucleic acids research

authors： Kagawa TF,Howell ML,Tseng K,Ho PS

更新日期：1993-12-25 00:00:00

abstract：:A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplific...

journal_title：Nucleic acids research

authors： Bonnet S,Prévot G,Bourgouin C

更新日期：1998-02-15 00:00:00

abstract：:The X chromosome-linked inhibitor of apoptosis protein (XIAP) is the most potent intrinsic caspase inhibitor and plays an important role in the maintenance of intestinal epithelial integrity. The RNA binding protein, HuR, regulates the stability and translation of many target transcripts. Here, we report that HuR asso...

journal_title：Nucleic acids research

authors： Zhang X,Zou T,Rao JN,Liu L,Xiao L,Wang PY,Cui YH,Gorospe M,Wang JY

更新日期：2009-12-01 00:00:00

abstract：:A novel cytoplasmic gene expression system has been developed. This system differs from other expression systems in that it relies on the co-delivery of plasmid DNA and T7 RNA polymerase (RNAP) during transfection. The plasmid contains a T7 RNAP gene driven by the T7 promoter (T7 autogene) and a functional/reporter ge...

journal_title：Nucleic acids research

authors： Chen X,Li Y,Xiong K,Wagner TE

更新日期：1994-06-11 00:00:00

abstract：:Biological pathways can provide key information on the organization of biological systems. ArrayXPath (http://www.snubi.org/software/ArrayXPath/) is a web-based service for mapping and visualizing microarray gene-expression data for integrated biological pathway resources using Scalable Vector Graphics (SVG). By integ...

journal_title：Nucleic acids research

authors： Chung HJ,Kim M,Park CH,Kim J,Kim JH

更新日期：2004-07-01 00:00:00

abstract：:The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a t...

journal_title：Nucleic acids research

authors： Chrisey LA,Lee GU,O'Ferrall CE

更新日期：1996-08-01 00:00:00

abstract：:RNA editing of miRNAs, especially in the seed region, adds another layer to miRNA mediated gene regulation which can modify its targets, altering cellular signaling involved in important processes such as differentiation. In this study, we have explored the role of miRNA editing in CD4(+) T cell differentiation. CD4(+...

journal_title：Nucleic acids research

authors： Negi V,Paul D,Das S,Bajpai P,Singh S,Mukhopadhyay A,Agrawal A,Ghosh B

更新日期：2015-09-18 00:00:00

abstract：:The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and overexpressed it in E. coli. Upon induction with IPTG, M.MspI constitutes...

journal_title：Nucleic acids research

authors： Dubey AK,Mollet B,Roberts RJ

更新日期：1992-04-11 00:00:00

abstract：:During the last years, the demand for custom-made cDNA chips/arrays as well as whole genome chips is increasing rapidly. The efficient selection of gene-specific primers/oligomers is of the utmost importance for the successful production of such chips. We developed GenomePRIDE, a highly flexible and scalable software ...

journal_title：Nucleic acids research

authors： Haas SA,Hild M,Wright AP,Hain T,Talibi D,Vingron M

更新日期：2003-10-01 00:00:00

abstract：:Microbial functional diversification is driven by environmental factors, i.e. microorganisms inhabiting the same environmental niche tend to be more functionally similar than those from different environments. In some cases, even closely phylogenetically related microbes differ more across environments than across tax...

journal_title：Nucleic acids research

authors： Zhu C,Mahlich Y,Miller M,Bromberg Y

更新日期：2018-01-04 00:00:00

abstract：:Struct2Net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. Prediction of protein-protein interactions (PPIs) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of t...

journal_title：Nucleic acids research

authors： Singh R,Park D,Xu J,Hosur R,Berger B

更新日期：2010-07-01 00:00:00

abstract：:Although subtelomeric regions in humans are heterochromatic, the epigenetic nature of human telomeres remains controversial. This controversy might have been influenced by the confounding effect of subtelomeric regions and interstitial telomeric sequences (ITSs) on telomeric chromatin structure analyses. In addition, ...

journal_title：Nucleic acids research

authors： Cubiles MD,Barroso S,Vaquero-Sedas MI,Enguix A,Aguilera A,Vega-Palas MA

更新日期：2018-03-16 00:00:00

abstract：:SSCP and heteroduplex analysis (HA) continue to be the most popular methods of mutation detection due to their simplicity, high sensitivity and low cost. The advantages of these methods are most clearly visible when large genes, such as BRCA1 and BRCA2, are scanned for scattered unknown mutations and/or when a large n...

journal_title：Nucleic acids research

authors： Kozlowski P,Krzyzosiak WJ

更新日期：2001-07-15 00:00:00

abstract：:N7-Methyl-2'-deoxyguanosine (m7dG) is the predominant lesion formed by methylating agents. A systematic investigation on the effect of m7dG on DNA replication has been difficult due to the chemical instability of m7dG. To gain insights into the m7dG effect, we employed a 2'-fluorine-mediated transition-state destabilz...

journal_title：Nucleic acids research

authors： Koag MC,Kou Y,Ouzon-Shubeita H,Lee S

更新日期：2014-07-01 00:00:00

abstract：:The tissue-specific expression of the Drosophila beta 2 tubulin gene ( B2t ) is accomplished by the action of a 14-bp activator element (beta2UE1) in combination with certain regulatory elements of the TATA-less, Inr-containing B2t core promoter. We performed an in vivo analysis of the Inr element function in the B2t ...

journal_title：Nucleic acids research

authors： Santel A,Kaufmann J,Hyland R,Renkawitz-Pohl R

更新日期：2000-03-15 00:00:00

abstract：:Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...

journal_title：Nucleic acids research

authors： Schäfer M,Kunz W

更新日期：1985-02-25 00:00:00

abstract：:Intramolecular RNA cross-links were induced within the large ribosomal subunit of E. coli by mild ultraviolet irradiation. Regions of the 23S RNA previously implicated in interactions with ribosomal-bound tRNA were then specifically excised by addressed cleavage using ribonuclease H, in conjunction with synthetic comp...

journal_title：Nucleic acids research

authors： Mitchell P,Osswald M,Schueler D,Brimacombe R

更新日期：1990-08-11 00:00:00

abstract：:Although telomeres are not recognized as double-strand breaks (DSBs), some DSB repair proteins are present at telomeres and are required for telomere maintenance. To learn more about the telomeric function of proteins from the homologous recombination (HR) and non-homologous end joining pathways (NHEJ), we have screen...

journal_title：Nucleic acids research

authors： Wei C,Skopp R,Takata M,Takeda S,Price CM

更新日期：2002-07-01 00:00:00

abstract：:Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, ...

journal_title：Nucleic acids research

authors： Miyoshi K,Arentzen R,Huang T,Itakura K

更新日期：1980-11-25 00:00:00

abstract：:Our database on large ribosomal subunit RNA contained 334 sequences in July, 1995. All sequences in the database are aligned, taking into account secondary structure. The aligned sequences are provided, together with incorporated secondary structure information, in several computer-readable formats. These data can eas...

journal_title：Nucleic acids research

authors： De Rijk P,Van de Peer Y,De Wachter R

更新日期：1996-01-01 00:00:00

abstract：:Gene losses provide an insightful route for studying the morphological and physiological adaptations of species, but their discovery is challenging. Existing genome annotation tools focus on annotating intact genes and do not attempt to distinguish nonfunctional genes from genes missing annotation due to sequencing an...

journal_title：Nucleic acids research

authors： Turakhia Y,Chen HI,Marcovitz A,Bejerano G

更新日期：2020-09-18 00:00:00