Abstract:
:We have purified to near homogeneity a novel nuclear protein from HeLa cells, that specifically binds to scaffold or matrix attachment region DNA elements (S/MAR DNA). The protein, designated SAF-B for scaffold attachment factor B, is an abundant component of chromatin, but not of the nuclear matrix and is expressed in all human tissues investigated. Antibodies against the purified protein were raised in rabbit and used to isolate the complete cDNA encoding SAF-B by immunoscreening. As predicted from the cDNA sequence, SAF-B contains 849 amino acids (96 696 Da), without significant homology to any known protein. SAF-B is rich in charged residues, leading to an aberrant migration on SDS gels, and has two putative bipartite nuclear localisation signals.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Renz A,Fackelmayer FOdoi
10.1093/nar/24.5.843subject
Has Abstractpub_date
1996-03-01 00:00:00pages
843-9issue
5eissn
0305-1048issn
1362-4962pii
5w0223journal_volume
24pub_type
杂志文章abstract::Allostery is the most direct, rapid and efficient way of regulating protein function, ranging from the control of metabolic mechanisms to signal-transduction pathways. However, an enormous amount of unsystematic allostery information has deterred scientists who could benefit from this field. Here, we present the AlloS...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1022
更新日期:2011-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1062
更新日期:2008-03-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that mediate gene expression at the post-transcriptional and translational levels by an imperfect binding to target mRNA 3'UTR regions. While the ab-initio computational prediction of miRNA-mRNA interactions still poses significant challenges, it is possible to overcome som...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1239
更新日期:2010-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.3.695
更新日期:2000-02-01 00:00:00
abstract::A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described. The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-06-11 00:00:00
abstract::The photostability and narrow emission spectra of non-organic quantum dot fluorophores (QDs) make them desirable candidates for fluorescent in situ hybridization (FISH) to study the expression of specific mRNA transcripts. We developed a novel method for direct QD labeling of modified oligonucleotide probes through st...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gni162
更新日期:2005-10-13 00:00:00
abstract::Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.19.4563
更新日期:1993-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.8.1472
更新日期:1996-04-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.25.5978
更新日期:1993-12-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-02-15 00:00:00
abstract::The X chromosome-linked inhibitor of apoptosis protein (XIAP) is the most potent intrinsic caspase inhibitor and plays an important role in the maintenance of intestinal epithelial integrity. The RNA binding protein, HuR, regulates the stability and translation of many target transcripts. Here, we report that HuR asso...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp755
更新日期:2009-12-01 00:00:00
abstract::A novel cytoplasmic gene expression system has been developed. This system differs from other expression systems in that it relies on the co-delivery of plasmid DNA and T7 RNA polymerase (RNAP) during transfection. The plasmid contains a T7 RNAP gene driven by the T7 promoter (T7 autogene) and a functional/reporter ge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.11.2114
更新日期:1994-06-11 00:00:00
abstract::Biological pathways can provide key information on the organization of biological systems. ArrayXPath (http://www.snubi.org/software/ArrayXPath/) is a web-based service for mapping and visualizing microarray gene-expression data for integrated biological pathway resources using Scalable Vector Graphics (SVG). By integ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh476
更新日期:2004-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.15.3031
更新日期:1996-08-01 00:00:00
abstract::RNA editing of miRNAs, especially in the seed region, adds another layer to miRNA mediated gene regulation which can modify its targets, altering cellular signaling involved in important processes such as differentiation. In this study, we have explored the role of miRNA editing in CD4(+) T cell differentiation. CD4(+...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv752
更新日期:2015-09-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.7.1579
更新日期:1992-04-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg752
更新日期:2003-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1060
更新日期:2018-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq481
更新日期:2010-07-01 00:00:00
abstract::Although subtelomeric regions in humans are heterochromatic, the epigenetic nature of human telomeres remains controversial. This controversy might have been influenced by the confounding effect of subtelomeric regions and interstitial telomeric sequences (ITSs) on telomeric chromatin structure analyses. In addition, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-03-16 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku554
更新日期:2014-07-01 00:00:00
abstract::The tissue-specific expression of the Drosophila beta 2 tubulin gene ( B2t ) is accomplished by the action of a 14-bp activator element (beta2UE1) in combination with certain regulatory elements of the TATA-less, Inr-containing B2t core promoter. We performed an in vivo analysis of the Inr element function in the B2t ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.6.1439
更新日期:2000-03-15 00:00:00
abstract::Cloned ribosomal DNA (rDNA) of Locusta migratoria was analyzed by restriction site mapping and SI nuclease experiments. The repeat unit is 18 kb long. The nontranscribed spacer region (NTS) is very large (11 kb) and homogeneous in length, but many of the restriction sites are heterogeneous among the repeat units. Two ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.4.1251
更新日期:1985-02-25 00:00:00
abstract::Intramolecular RNA cross-links were induced within the large ribosomal subunit of E. coli by mild ultraviolet irradiation. Regions of the 23S RNA previously implicated in interactions with ribosomal-bound tRNA were then specifically excised by addressed cleavage using ribonuclease H, in conjunction with synthetic comp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.15.4325
更新日期:1990-08-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf396
更新日期:2002-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.22.5507
更新日期:1980-11-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.1.92
更新日期:1996-01-01 00:00:00
abstract::Gene losses provide an insightful route for studying the morphological and physiological adaptations of species, but their discovery is challenging. Existing genome annotation tools focus on annotating intact genes and do not attempt to distinguish nonfunctional genes from genes missing annotation due to sequencing an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa550
更新日期:2020-09-18 00:00:00