Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme.

abstract：:We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome c...

journal_title：Nucleic acids research

authors： Breindl M,Kalthoff H,Jaenisch R

更新日期：1983-06-25 00:00:00

abstract：:The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and tw...

journal_title：Nucleic acids research

authors： Rotheneder H,Grabner M,Wintersberger E

更新日期：1991-12-25 00:00:00

abstract：:It is informative to detect highly conserved positions in proteins and nucleic acid sequence/structure since they are often indicative of structural and/or functional importance. ConSurf (http://consurf.tau.ac.il) and ConSeq (http://conseq.tau.ac.il) are two well-established web servers for calculating the evolutionar...

journal_title：Nucleic acids research

authors： Ashkenazy H,Erez E,Martz E,Pupko T,Ben-Tal N

更新日期：2010-07-01 00:00:00

abstract：:We have previously shown that integration of a polyoma vector containing rodent repetitive elements into rat cellular DNA is non-random (Wallenburg et al. J. Virol. 50: 678-683). Junctions between the polyoma vector and the host DNA occur in the repetitive sequences of the vector about ten times more frequently than w...

journal_title：Nucleic acids research

authors： Wallenburg JC,Nepveu A,Chartrand P

更新日期：1987-10-12 00:00:00

abstract：:The response to amino acid (AA) limitation of the entire aminoacyl-tRNA synthetase (ARS) gene family revealed that 16/20 of the genes encoding cytoplasmic-localized enzymes are transcriptionally induced by activating transcription factor 4 (Atf4) via C/ebp-Atf-Response-Element (CARE) enhancers. In contrast, only 4/19 ...

journal_title：Nucleic acids research

authors： Shan J,Zhang F,Sharkey J,Tang TA,Örd T,Kilberg MS

更新日期：2016-11-16 00:00:00

abstract：:In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously s...

journal_title：Nucleic acids research

authors： Bostian KA,Burn VE,Jayachandran S,Tipper DJ

更新日期：1983-02-25 00:00:00

abstract：:Recently, we reported a multicopy mammalian plasmid with a structure related to polyoma. The plasmid, named L factor, was found at a high copy number (5,000 or more per cell) in a subclone derived from mouse L cells. We attempted to utilize L factor as a plasmid vector for mammalian cells. A series of composite DNA co...

journal_title：Nucleic acids research

authors： Saito H,Uehara H,Kusano T,Oishi M

更新日期：1987-11-11 00:00:00

abstract：:RNA processing is critical for proper spatial and temporal control of gene expression. The ubiquitous nuclear polyadenosine RNA binding protein, PABPN1, post-transcriptionally regulates multiple steps of gene expression. Mutations in the PABPN1 gene expanding an N-terminal alanine tract in the PABPN1 protein from 10 a...

journal_title：Nucleic acids research

authors： Phillips BL,Banerjee A,Sanchez BJ,Di Marco S,Gallouzi IE,Pavlath GK,Corbett AH

更新日期：2018-09-06 00:00:00

abstract：:A comprehensive listing is made of posttranscriptionally modified nucleosides from RNA reported in the literature through mid-1994. Included are chemical structures, common names, symbols, Chemical Abstracts registry numbers (for ribonucleoside and corresponding base), Chemical Abstracts Index Name, phylogenetic sourc...

journal_title：Nucleic acids research

authors： Limbach PA,Crain PF,McCloskey JA

更新日期：1994-06-25 00:00:00

abstract：:HotSpot Wizard is a web server used for the automated identification of hotspots in semi-rational protein design to give improved protein stability, catalytic activity, substrate specificity and enantioselectivity. Since there are three orders of magnitude fewer protein structures than sequences in bioinformatic datab...

journal_title：Nucleic acids research

authors： Sumbalova L,Stourac J,Martinek T,Bednar D,Damborsky J

更新日期：2018-07-02 00:00:00

abstract：:Highly repetitive Hind III restriction fragments of 0.72-0.76 KBP from total Xenopus laevis genomic DNA are organized in a tandem like arrangement. Cloning of these fragments in pBR 322 with subsequent restriction site mapping and nucleotide sequence analysis of some selected clones showed two different types of seque...

journal_title：Nucleic acids research

authors： Meyerhof W,Tappeser B,Korge E,Knöchel W

更新日期：1983-10-25 00:00:00

abstract：:Nuclear receptors for the thyroid hormone and vitamin A and D cooperate with the retinoid X receptor (RXR) in activating the transcription. Although the hormone response elements for these receptors have been proposed in which spacing of the direct repeated motifs determine the specificity (so called 3-4-5 rule), vita...

journal_title：Nucleic acids research

authors： Nishikawa J,Kitaura M,Matsumoto M,Imagawa M,Nishihara T

更新日期：1994-08-11 00:00:00

abstract：:Few transcriptional regulatory networks have been described in non-model organisms. In Entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. We used expression data from tw...

journal_title：Nucleic acids research

authors： Hackney JA,Ehrenkaufer GM,Singh U

更新日期：2007-01-01 00:00:00

abstract：:Ribosomal protein (RP) genes must be coordinately expressed for proper assembly of the ribosome yet the mechanisms that control expression of RP genes in metazoans are poorly understood. Recently, TATA-binding protein-related factor 2 (TRF2) rather than the TATA-binding protein (TBP) was found to function in transcrip...

journal_title：Nucleic acids research

authors： Baumann DG,Gilmour DS

更新日期：2017-10-13 00:00:00

abstract：:A mass culture of human diploid fibroblasts, and eight clones isolated from that mass culture, were examined for methylation patterns in several regions of DNA. Plasmid-inserted cDNA sequences were used as probes for alpha-hCG, beta-globin, A gamma- and G gamma-globin, and beta- and gamma-actin gene regions. Each prob...

journal_title：Nucleic acids research

authors： Shmookler Reis RJ,Goldstein S

更新日期：1982-07-24 00:00:00

abstract：:p16INK4a and p21WAF1, two major cyclin-dependent kinase inhibitors, are the products of two tumor suppressor genes that play important roles in various cellular metabolic pathways. p21WAF1 is up-regulated in response to different DNA damaging agents. While the activation of p21WAF1 is p53-dependent following -rays, th...

journal_title：Nucleic acids research

authors： Al-Mohanna MA,Al-Khalaf HH,Al-Yousef N,Aboussekhra A

更新日期：2007-01-01 00:00:00

abstract：:The EcoCyc database describes the genome and gene products of Escherichia coli, its metabolic and signal-transduction pathways, and its tRNAs. The database describes 4391 genes of E.coli, 695 enzymes encoded by a subset of these genes, 904 metabolic reactions that occur in E.coli, and the organization of these reactio...

journal_title：Nucleic acids research

authors： Karp PD,Riley M,Paley SM,Pellegrini-Toole A,Krummenacker M

更新日期：1999-01-01 00:00:00

abstract：:DNA tandem repeats (TRs) are ubiquitous genomic features which consist of two or more adjacent copies of an underlying pattern sequence. The copies may be identical or approximate. Variable number of tandem repeats or VNTRs are polymorphic TR loci in which the number of pattern copies is variable. In this paper we des...

journal_title：Nucleic acids research

authors： Gelfand Y,Hernandez Y,Loving J,Benson G

更新日期：2014-08-01 00:00:00

abstract：:With the recent increase in the number of mammalian genomes being sequenced, large-scale genome scans for human-specific positive selection are now possible. Selection can be inferred through phylogenetic analysis by comparing the rates of silent and replacement substitution between related species. Maximum-likelihood...

journal_title：Nucleic acids research

authors： Nickel GC,Tefft D,Adams MD

更新日期：2008-01-01 00:00:00

abstract：:Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with 13C and 15N. Imino proton signals were assigned by analysis...

journal_title：Nucleic acids research

authors： Tanaka Y,Hori T,Tagaya M,Sakamoto T,Kurihara Y,Katahira M,Uesugi S

更新日期：2002-02-01 00:00:00

abstract：:Comparisons of proteins show that they evolve through the movement of domains. However, in many cases, the underlying mechanisms remain unclear. Here, we observed the movements of DNA recognition domains between non-orthologous proteins within a prokaryote genome. Restriction-modification (RM) systems, consisting of a...

journal_title：Nucleic acids research

authors： Furuta Y,Kobayashi I

更新日期：2012-10-01 00:00:00

abstract：:The Raf-1 proto-oncogene product is a highly regulated serine/threonine kinase that functions in signal transduction downstream from growth factor receptors and upstream from nuclear proto-oncogene products. Using a transient cotransfection assay we have found that activated Raf-1 activates expression from the HIV-LTR...

journal_title：Nucleic acids research

authors： Bruder JT,Heidecker G,Tan TH,Weske JC,Derse D,Rapp UR

更新日期：1993-11-11 00:00:00

abstract：:Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which p...

journal_title：Nucleic acids research

authors： Arnott S,Bond PJ,Selsing E,Smith PJ

更新日期：1976-10-01 00:00:00

abstract：:Eukaryotic cells possess several distinct mismatch repair pathways. A mismatch can be introduced in retroviral double-stranded DNA by a pre-existing mutation within the primer binding site (PBS) of the viral RNA genome. In order to evaluate mismatch repair of retroviral double-stranded DNA, Moloney leukemia virus (MLV...

journal_title：Nucleic acids research

authors： Tang LY,Zhang J

更新日期：2000-06-15 00:00:00

abstract：:The sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA has been determined. The sequence contains the entire coat protein cistron as well as regions to its left and right. Sequence characterization was by conventional methods for use with uniformly 32P labeled RNA complemented by newer methods for ...

journal_title：Nucleic acids research

authors： Guilley H,Jonard G,Kukla B,Richards KE

更新日期：1979-04-01 00:00:00

abstract：:Bursts of free radicals produced by ionization of water in close vicinity to DNA can produce clusters of opposed DNA lesions and these are termed multiply damaged sites (MDS). How MDS are processed by the Escherichia coli DNA glycosylases, endonuclease (endo) III and endo VIII, which recognize oxidized pyrimidines, is...

journal_title：Nucleic acids research

authors： Harrison L,Hatahet Z,Purmal AA,Wallace SS

更新日期：1998-02-15 00:00:00

abstract：:TcruziDB (http://TcruziDB.org) is an integrated genome database for the parasitic organism Trypanosoma cruzi, the causative agent of Chagas' disease. The database currently incorporates all available sequence data (Genomic, BAC, EST) in a single user-friendly location. The database contains a variety of tools specific...

journal_title：Nucleic acids research

authors： Luchtan M,Warade C,Weatherly DB,Degrave WM,Tarleton RL,Kissinger JC

更新日期：2004-01-01 00:00:00

abstract：:The G- and U-specific reagent, carbodiimide was used to probe the solution structure of aminoacylated yeast phenylalanine tRNA. Both quantitative and qualitative changes in modification were observed when the modification patterns of tRNA-CCA(3'OH), tRNA-CCA(3'NH2) and phe-tRNA-CCA(3'NH2) were compared. Five nucleotid...

journal_title：Nucleic acids research

authors： Fritzinger DC,Fournier MJ

更新日期：1982-04-10 00:00:00

abstract：:Organisms exposed to reactive oxygen species, generated endogenously during respiration or by environmental conditions, undergo oxidative stress. Stress response can either repair the damage or activate one of the programmed cell death (PCD) mechanisms, for example apoptosis, and finally end in cell death. One strikin...

journal_title：Nucleic acids research

authors： Mroczek S,Kufel J

更新日期：2008-05-01 00:00:00

abstract：:Mitochondrial proteins remain the subject of intense research interest due to their implication in an increasing number of different conditions including mitochondrial and metabolic disease, cancer, and neuromuscular degenerative and age-related disorders. However, the mitochondrial proteome has yet to be accurately a...

journal_title：Nucleic acids research

authors： Smith AC,Robinson AJ

更新日期：2016-01-04 00:00:00