Abstract：
:In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously shown to be identical to ScV-P1, the 88,000 dalton major capsid protein of the virus-like particles containing L- and M1-dsRNAs. 1, but not 1a, is bound to poly(U)-sepharose and may be polyadenylated. Other L-dsRNA gene products and their transcripts may exist. For M1-dsRNA, both species (m, ma) have in vitro mRNA activity for M1-P1, the 32,000 dalton pre-protoxin encoded by M1-dsRNA. Both m and ma are bound to poly(U)-Sepharose and ma is probably a 5' terminal fragment of m. A functional model for M1-dsRNA killer plasmid structure is presented.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bostian KA,Burn VE,Jayachandran S,Tipper DJdoi
10.1093/nar/11.4.1077subject
Has Abstractpub_date
1983-02-25 00:00:00pages
1077-97issue
4eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract：:Knowledge of the various interactions between molecules in the cell is crucial for understanding cellular processes in health and disease. Currently available interaction databases, being largely complementary to each other, must be integrated to obtain a comprehensive global map of the different types of interactions...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1055
更新日期：2013-01-01 00:00:00
abstract：:Recent studies show that the region of DNA brought into close proximity to the fetal gamma-globin genes in deletional forms of hereditary persistence of fetal hemoglobin (HPFH) is selectively hypomethylated (and presumably active) in normal erythroid tissue. This region is normally located approximately 100 kilobases ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.13.5169
更新日期：1987-07-10 00:00:00
abstract：:The principles of mRNA decoding are conserved among all extant life forms. We present an integrative view of all the interaction networks between mRNA, tRNA and rRNA: the intrinsic stability of codon-anticodon duplex, the conformation of the anticodon hairpin, the presence of modified nucleotides, the occurrence of no...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkw608
更新日期：2016-09-30 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz265
更新日期：2019-06-04 00:00:00
abstract：:Single-molecule force spectroscopy provides insight into how proteins bind to and move along DNA. Such studies often embed a single-stranded (ss) DNA region within a longer double-stranded (ds) DNA molecule. Yet, producing these substrates remains laborious and inefficient, particularly when using the traditional thre...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx761
更新日期：2017-10-13 00:00:00
abstract：:Eukaryotes produce multiple products from a single gene locus by alternative splicing, translation or promoter usage as mechanisms expanding the complexity of their proteome. Trithorax proteins, including the Arabidopsis Trithorax-like protein ATX1, are histone modifiers regulating gene activity. Here, we report that ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1300
更新日期：2011-06-01 00:00:00
abstract：:RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.23.7763
更新日期：1982-12-11 00:00:00
abstract：:We describe the first polyA-containing processed pseudogene reported in the chicken. It includes a 0.52 kb open reading frame which could encode a 175 amino acid protein. The putative protein shows extensive homology to the ras oncogene superfamily, being most closely related to the yeast protein YP2. It is one of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.5.2123
更新日期：1986-03-11 00:00:00
abstract：:DNA polymerases of the A and B families, and reverse transcriptases, share a common mechanism for preventing incorporation of ribonucleotides: a highly conserved active site residue obstructing the position that would be occupied by a 2' hydroxyl group on the incoming nucleotide. In the family Y (lesion bypass) polyme...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg417
更新日期：2003-07-15 00:00:00
abstract：:Bacterial small regulatory RNAs (sRNAs) are the most abundant class of post-transcriptional regulators and have been well studied in Gram-negative bacteria. Little is known about the functions and mechanisms of sRNAs in high GC Gram-positive bacteria including Mycobacterium and Streptomyces. Here, we performed an in-d...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz149
更新日期：2019-05-07 00:00:00
abstract：:The mitochondrial DNA of Neurospora crassa has a heterogeneous intramolecular base distribution. A contiguous piece, representing at least 30% of the total genome, has a G+C content that is 6% lower than the overall G+C content of the DNA. The genes for both ribosomal RNAs are contained in the remaining, relatively G+...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.1.129
更新日期：1977-01-01 00:00:00
abstract：:A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in the following order: (5') H2B25,35-H455,65-H375,85,95/H488-H2B105,11 5-H2A118-H3135,145/H2A145 (3') (the subscripts giv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.10.3439
更新日期：1985-05-24 00:00:00
abstract：:Addition of thiol compounds containing an anionic group to the 3'-terminal unsaturated sugar of the 5' fragment obtained from an oligonucleotide containing an AP site cleaved by beta-elimination, can be followed by gel electrophoresis. The technique enables to distinguish between two mechanisms of cleavage of the C3'-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.20.9489
更新日期：1988-10-25 00:00:00
abstract：:The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcrip...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.24.5570
更新日期：1993-12-11 00:00:00
abstract：:We describe an improved model of DNA replication in Xenopus egg extracts, in which a circular plasmid immobilized on paramagnetic beads is used as a template. DNA synthesis occurred on either circular or linear plasmids coupled to the beads, but only DNA synthesis on the circular plasmid was inhibited by geminin and a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl512
更新日期：2006-07-26 00:00:00
abstract：:Abasic sites (AP-sites) are frequent DNA lesions, arising by spontaneous base hydrolysis or as intermediates of base excision repair (BER). The hemiacetal at the anomeric centre renders them chemically reactive, which presents a challenge to biochemical and structural investigation. Chemically more stable AP-site anal...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1520
更新日期：2016-03-18 00:00:00
abstract：:The use of the DNA duplex as a supramolecular scaffold is an established approach for the assembly of chromophore aggregates. In the absence of detailed structural insight, the characterization of thus assembled oligochromophores is, today, largely based on solution-phase spectroscopy. Here, we describe the crystal st...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw644
更新日期：2016-09-06 00:00:00
abstract：:GC-AG introns represent 0.7% of total human pre-mRNA introns. To study the function of GC-AG introns in splicing regulation, 196 cDNA-confirmed GC-AG introns were identified in Caenorhabditis elegans. These represent 0.6% of the cDNA- confirmed intron data set for this organism. Eleven of these GC-AG introns are invol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf465
更新日期：2002-08-01 00:00:00
abstract：:Large expansions of the trinucleotide repeat GAA*TTC within the first intron of the X25 (frataxin) gene cause Friedreich's ataxia, the most common inherited ataxia. Expansion leads to reduced levels of frataxin mRNA in affected individuals. Here we show that GAA*TTC tracts, in the absence of any other frataxin gene se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.14.2815
更新日期：2000-07-15 00:00:00
abstract：:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisel...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.8.2325
更新日期：1983-04-25 00:00:00
abstract：:Transcription and translation are coupled in bacteria, meaning that translation takes place co-transcriptionally. During transcription-translation, both machineries mutually affect each others' functions, which is important for regulation of gene expression. Analysis of interactions between RNA polymerase (RNAP) and t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1262
更新日期：2012-03-01 00:00:00
abstract：:Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq769
更新日期：2011-01-01 00:00:00
abstract：:In plants, nuclear multisubunit RNA polymerases IV and V are RNA Polymerase II-related enzymes that synthesize non-coding RNAs for RNA-directed DNA methylation (RdDM) and transcriptional gene silencing. Here, we tested the importance of the C-terminal domain (CTD) of Pol IV's largest subunit given that the Pol II CTD ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz615
更新日期：2019-09-26 00:00:00
abstract：:We have demonstrated that the DNA sequence between two triplex-forming polypurine.polypyrimidine (Pu.Py) tracts was protected from DNA modifying enzymes upon formation of triplex DNA structures with an oligodeoxyribonucleotide in which two triplex-forming Pu or Py tracts were placed at the termini (triplex-bridge form...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.3.452
更新日期：1995-02-11 00:00:00
abstract：:Alternative splicing expands the coding capacity of metazoan genes, and it was largely genetic studies in the fruit-fly Drosophila melanogaster that established the principle that regulated alternative splicing results in tissue- and stage-specific protein isoforms with different functions in development. Alternative ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr648
更新日期：2012-01-01 00:00:00
abstract：:Sequence heterogeneity is a common characteristic of RNA viruses that is often referred to as sub-populations or quasispecies. Traditional techniques used for assembly of short sequence reads produced by deep sequencing, such as de-novo assemblers, ignore the underlying diversity. Here, we introduce a novel algorithm ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx755
更新日期：2017-11-02 00:00:00
abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.6.e33
更新日期：2001-03-15 00:00:00
abstract：:The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along with analysis services and a phylogenetically consistent taxonomic framework for these data. Updated monthly, these services are made available through the RDP-II website (http://rdp.cme.msu.ed...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki038
更新日期：2005-01-01 00:00:00
abstract：:Eukaryotic cells use numerous pathways to regulate RNA production, localization and stability. Several of these pathways are controlled by ribonucleases. The essential ribonuclease, Dis3, plays important roles in distinct RNA metabolic pathways. Despite much progress in understanding general characteristics of the Dis...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq295
更新日期：2010-09-01 00:00:00
abstract：:Many bacterial plasmids replicate by an asymmetric rolling-circle mechanism that requires sequence-specific recognition for initiation, nicking of one of the template DNA strands and unwinding of the duplex prior to subsequent leading strand DNA synthesis. Nicking is performed by a replication-initiation protein (Rep)...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw689
更新日期：2016-10-14 00:00:00
