A processed chicken pseudogene (CPS1) related to the ras oncogene superfamily.

abstract：:A series of plasmids were constructed in which a promoter was introduced into a lac-based operon fusion vector. A perfectly symmetrical oligonucleotide of 22-bp corresponding to an idealized binding site for cAMP receptor protein (CRP) of E. coli was chemically synthesized. The synthetic CRP site was placed between th...

journal_title：Nucleic acids research

authors： Morita T,Shigesada K,Kimizuka F,Aiba H

更新日期：1988-08-11 00:00:00

abstract：:The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction during transposition. In this study, comparative DNA-binding analyses were performed using purified full-length Tnp and a C-terminal deletion variant (delta369) that lacks the putative dimerization domain. The shape of t...

journal_title：Nucleic acids research

authors： York D,Reznikoff WS

更新日期：1996-10-01 00:00:00

abstract：:The Epstein-Barr virus gene BRLF1 encodes the transcription factor R, which is a sequence-specific DNA-binding protein important for the switch from latency to a productive cycle. We have defined a repertoire of specific R-binding sites using a GST-R fusion protein and a pool of 23 bp random DNA sequences. The R-bound...

journal_title：Nucleic acids research

authors： Gruffat H,Sergeant A

更新日期：1994-04-11 00:00:00

abstract：:A 15-residue sequence motif has been found in many polymerases from various species and involving DNA and RNA dependence and product. The motif is characterized by a Tyr-Gly-Asp-(Thr)-Asp core flanked by hydrophobic spans five residues in length. An mRNA maturase segment is also suggested to display the motif pattern....

journal_title：Nucleic acids research

authors： Argos P

更新日期：1988-11-11 00:00:00

abstract：:Acinetobacter sp. strain ADP1 is a nutritionally versatile soil bacterium closely related to representatives of the well-characterized Pseudomonas aeruginosa and Pseudomonas putida. Unlike these bacteria, the Acinetobacter ADP1 is highly competent for natural transformation which affords extraordinary convenience for ...

journal_title：Nucleic acids research

authors： Barbe V,Vallenet D,Fonknechten N,Kreimeyer A,Oztas S,Labarre L,Cruveiller S,Robert C,Duprat S,Wincker P,Ornston LN,Weissenbach J,Marlière P,Cohen GN,Médigue C

更新日期：2004-10-28 00:00:00

abstract：:GAP-43 is a membrane phosphoprotein that is important for the development and plasticity of neural connections. In undifferentiated PC12 pheochromocytoma cells, GAP-43 mRNA degrades rapidly ( t = 5 h), but becomes stable when cells are treated with nerve growth factor. To identify trans- acting factors that may influe...

journal_title：Nucleic acids research

authors： Irwin N,Baekelandt V,Goritchenko L,Benowitz LI

更新日期：1997-03-15 00:00:00

abstract：:Mitochondrial DNA from human tissue culture cells contains heterogeneous sequences located within a previously identified, evolutionarily conserved region termed CSB-2. CSB-2 is located near the origin of heavy-strand mitochondrial DNA synthesis and the major transcriptional promoters for each strand of human mitochon...

journal_title：Nucleic acids research

authors： Hauswirth WW,Clayton DA

更新日期：1985-11-25 00:00:00

abstract：:Recently, we developed a mass DNA-mediated transformation technique for the ciliated protozoan Tetrahymena thermophila that introduces transforming DNA by electroporation into conjugating cells. Other studies demonstrated that a neomycin resistance gene flanked by Tetrahymena H4-I gene regulatory sequences transformed...

journal_title：Nucleic acids research

authors： Gaertig J,Gu L,Hai B,Gorovsky MA

更新日期：1994-12-11 00:00:00

abstract：:We show herein the transcription of a processed gene that originated from a spliced transcript. Recently, we isolated the human and murine homologues of the yeast chromatin protein, SPT4. The Supt4h gene is spliced normally from five exons encoded by chromosome 11. Here we show that a related sequence on chromosome 10...

journal_title：Nucleic acids research

authors： Chiang PW,Zhang R,Stubbs L,Zhang L,Zhu L,Kurnit DM

更新日期：1998-11-01 00:00:00

abstract：:In vitro splicing of human beta-globin pre-mRNA can be fully inhibited by treatment of the splicing extract with polyclonal antibodies against hnRNP core proteins prior to the addition of pre-mRNA. Inhibition of the first step in the splicing pathway, cleavage at the 5' splice site and lariat formation, requires more ...

journal_title：Nucleic acids research

authors： Sierakowska H,Szer W,Furdon PJ,Kole R

更新日期：1986-07-11 00:00:00

abstract：:The Immune Epitope Database Analysis Resource (IEDB-AR, http://tools.iedb.org/) is a companion website to the IEDB that provides computational tools focused on the prediction and analysis of B and T cell epitopes. All of the tools are freely available through the public website and many are also available through a RE...

journal_title：Nucleic acids research

authors： Dhanda SK,Mahajan S,Paul S,Yan Z,Kim H,Jespersen MC,Jurtz V,Andreatta M,Greenbaum JA,Marcatili P,Sette A,Nielsen M,Peters B

更新日期：2019-07-02 00:00:00

abstract：:The Human Gene and Protein Database (HGPD; http://www.HGPD.jp/) is a unique database that stores information on a set of human Gateway entry clones in addition to protein expression and protein synthesis data. The HGPD was launched in November 2008, and 33,275 human Gateway entry clones have been constructed from the ...

journal_title：Nucleic acids research

authors： Maruyama Y,Kawamura Y,Nishikawa T,Isogai T,Nomura N,Goshima N

更新日期：2012-01-01 00:00:00

abstract：:The chemical shifts of the well-resolved ring nitrogen protons of uracil and a series of substituted uracils, including pseudouridine (5-ribosyl-uracil) and uridine (1-ribosyl-uracil) were determined using nuclear magnetic resonance (NMR). The observed chemical shifts suggest the existence of an atypical syn conformat...

journal_title：Nucleic acids research

authors： Hurd RE,Reid BR

更新日期：1977-08-01 00:00:00

abstract：:African trypanosomes are extracellular protozoan parasites that are transmitted from one mammalian host to the next by tsetse flies. Bloodstream forms express variant surface glycoprotein (VSG); the tsetse fly (procyclic) forms express instead the procyclic acidic repetitive protein (PARP). PARP mRNA is abundant in pr...

journal_title：Nucleic acids research

authors： Biebinger S,Rettenmaier S,Flaspohler J,Hartmann C,Peña-Diaz J,Wirtz LE,Hotz HR,Barry JD,Clayton C

更新日期：1996-04-01 00:00:00

abstract：:MicroRNAs are a class of small non-coding RNAs that serve as important regulators of gene expression at the posttranscriptional level. They are stable in body fluids and pose great potential to serve as biomarkers. Here, we present a highly specific, sensitive and cost-effective system to quantify miRNA expression bas...

journal_title：Nucleic acids research

authors： Androvic P,Valihrach L,Elling J,Sjoback R,Kubista M

更新日期：2017-09-06 00:00:00

abstract：:The APOBEC3 proteins are unique to mammals. Many inhibit retrovirus infection through a cDNA cytosine deamination mechanism. HIV-1 neutralizes this host defense through Vif, which triggers APOBEC3 ubiquitination and degradation. Here, we report an APOBEC3F-like, double deaminase domain protein from three artiodactyls:...

journal_title：Nucleic acids research

authors： Jónsson SR,Haché G,Stenglein MD,Fahrenkrug SC,Andrésdóttir V,Harris RS

更新日期：2006-01-01 00:00:00

abstract：:Sequencing by hybridization (SBH) approaches to DNA sequencing face two conflicting constraints. First, in order to ensure that the target DNA binds reliably, the oligonucleotide probes that are attached to the chip array must be >15 bp in length. Secondly, the total number of possible 15 bp oligonucleotides is too la...

journal_title：Nucleic acids research

authors： Fedrigo O,Naylor G

更新日期：2004-02-18 00:00:00

abstract：:Full-length cDNA of the 6.3 kb turnip yellow mosaic virus (TYMV) genome was placed between a T7 promoter and a unique Hind III site. In vitro transcription of Hind III-linearized DNA of clone pTYMC yielded full-length RNA transcripts. In inoculations of Chinese cabbage protoplasts and plants, capped transcripts and vi...

journal_title：Nucleic acids research

authors： Weiland JJ,Dreher TW

更新日期：1989-06-26 00:00:00

abstract：:Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional charact...

journal_title：Nucleic acids research

authors： Benders GA,Noskov VN,Denisova EA,Lartigue C,Gibson DG,Assad-Garcia N,Chuang RY,Carrera W,Moodie M,Algire MA,Phan Q,Alperovich N,Vashee S,Merryman C,Venter JC,Smith HO,Glass JI,Hutchison CA 3rd

更新日期：2010-05-01 00:00:00

abstract：:Comparison of the nucleotide sequence of mRNAs coding for several vertebrate actins revealed a high degree of sequence homology in the 3' untranslated region (3' UTR) between those mRNAs coding for homologous (isotypic) actins in different organisms but not between mRNAs coding for very similar isoforms differing in t...

journal_title：Nucleic acids research

authors： Yaffe D,Nudel U,Mayer Y,Neuman S

更新日期：1985-05-24 00:00:00

abstract：:A common aim in ChIP-seq experiments is to identify changes in protein binding patterns between conditions, i.e. differential binding. A number of peak- and window-based strategies have been developed to detect differential binding when the regions of interest are not known in advance. However, careful consideration o...

journal_title：Nucleic acids research

authors： Lun AT,Smyth GK

更新日期：2014-06-01 00:00:00

abstract：:Genomic meta-analysis to combine relevant and homogeneous studies has been widely applied, but the quality control (QC) and objective inclusion/exclusion criteria have been largely overlooked. Currently, the inclusion/exclusion criteria mostly depend on ad-hoc expert opinion or naïve threshold by sample size or platfo...

journal_title：Nucleic acids research

authors： Kang DD,Sibille E,Kaminski N,Tseng GC

更新日期：2012-01-01 00:00:00

abstract：:Escherichia coli has long been regarded as a model organism in the study of codon usage bias (CUB). However, most studies in this organism regarding this topic have been computational or, when experimental, restricted to small datasets; particularly poor attention has been given to genes with low CUB. In this work, co...

journal_title：Nucleic acids research

authors： dos Reis M,Wernisch L,Savva R

更新日期：2003-12-01 00:00:00

abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...

journal_title：Nucleic acids research

authors： Philippe L,Pandarakalam GC,Fasimoye R,Harrison N,Connolly B,Pettitt J,Müller B

更新日期：2017-08-21 00:00:00

abstract：:The spliceosomal protein Prp1 (Prp6/U5-102 K) is necessary for the integrity of pre-catalytic spliceosomal complexes. We have identified a novel regulatory function for Prp1. Expression of mutations in the N-terminus of Prp1 leads to the accumulation of pre-catalytic spliceosomal complexes containing the five snRNAs U...

journal_title：Nucleic acids research

authors： Lützelberger M,Bottner CA,Schwelnus W,Zock-Emmenthal S,Razanau A,Käufer NF

更新日期：2010-03-01 00:00:00

abstract：:We report the first isolation and characterisation of a human Ig V lambda gene. The gene was isolated from a recombinant phage library of human chromosome 22 using a mouse Ig lambda cDNA as probe. DNA sequence analysis predicts a short leader peptide interrupted by an intron of 88 nucleotides, and a mature polypeptide...

journal_title：Nucleic acids research

authors： Anderson ML,Szajnert MF,Kaplan JC,McColl L,Young BD

更新日期：1984-09-11 00:00:00

abstract：:Carbohydrate-binding proteins play crucial roles across all organisms and viruses. The complexity of carbohydrate structures, together with inconsistencies in how their 3D structures are reported, has led to difficulties in characterizing the protein-carbohydrate interfaces. In order to better understand protein-carbo...

journal_title：Nucleic acids research

authors： Copoiu L,Torres PHM,Ascher DB,Blundell TL,Malhotra S

更新日期：2020-01-08 00:00:00

abstract：:We describe a simple procedure for photolithographic patterning of streptavidin on silicon substrates. Long wavelength UV (365 nm) light was used to direct the covalent attachment of photoactivatable biotin onto silylated silicon wafers. Fluorescently labeled streptavidin was found to bind only in areas exposed to the...

journal_title：Nucleic acids research

authors： Sabanayagam CR,Smith CL,Cantor CR

更新日期：2000-04-15 00:00:00

abstract：:The mitochondrial DNA (mtDNA) control regions for common chimpanzee, pygmy chimpanzee and gorilla were sequenced and the lengths and termini of their D-loop DNA's characterized. In these and all other species for which there are data, 5' termini map to sequences that contain the trinucleotide YAY. 3' termini are 25-51...

journal_title：Nucleic acids research

authors： Foran DR,Hixson JE,Brown WM

更新日期：1988-07-11 00:00:00

abstract：:Polyadenylation is a co-transcriptional process that modifies mRNA 3'-ends in eukaryotes. In yeast, CF IA and CPF constitute the core 3'-end maturation complex. CF IA comprises Rna14p, Rna15p, Pcf11p and Clp1p. CF IA interacts with the C-terminal domain of RNA Pol II largest subunit via Pcf11p which links pre-mRNA 3'-...

journal_title：Nucleic acids research

authors： Haddad R,Maurice F,Viphakone N,Voisinet-Hakil F,Fribourg S,Minvielle-Sébastia L

更新日期：2012-02-01 00:00:00