Force and twist dependence of RepC nicking activity on torsionally-constrained DNA molecules.

abstract：:Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. The CORUM (http://mips.gsf.de/genre/proj/corum/index.html) database is a collection of experimentally verified mammalian protein complexes. Information is manually derived by critical reading of the scient...

journal_title：Nucleic acids research

authors： Ruepp A,Brauner B,Dunger-Kaltenbach I,Frishman G,Montrone C,Stransky M,Waegele B,Schmidt T,Doudieu ON,Stümpflen V,Mewes HW

更新日期：2008-01-01 00:00:00

abstract：:Methylation of the 2'-hydroxyl-group of ribonucleotides is found in all major classes of RNA in eukaryotes and is one of the most abundant posttranscriptional modifications of stable RNAs. In spite of intense studies, the multiple functions of RNA 2'-O-methylation are still not understood. One major obstacle in the fi...

journal_title：Nucleic acids research

authors： Aschenbrenner J,Marx A

更新日期：2016-05-05 00:00:00

abstract：:Traditional Chinese medicine (TCM) is not only an effective solution for primary health care, but also a great resource for drug innovation and discovery. To meet the increasing needs for TCM-related data resources, we developed ETCM, an Encyclopedia of Traditional Chinese Medicine. ETCM includes comprehensive and sta...

journal_title：Nucleic acids research

authors： Xu HY,Zhang YQ,Liu ZM,Chen T,Lv CY,Tang SH,Zhang XB,Zhang W,Li ZY,Zhou RR,Yang HJ,Wang XJ,Huang LQ

更新日期：2019-01-08 00:00:00

abstract：:Anisotropic motions of DNA fragments in the size range 6-118 base pairs are studied by the steady-state fluorescence polarization of different excitation transitions in the intercalated ethidium cation. Calculated effective tumbling and twisting times are found to be shorter than predicted for overall motions of rigid...

journal_title：Nucleic acids research

authors： Härd T,Kearns DR

更新日期：1986-05-12 00:00:00

abstract：:In most eukaryotes, genetic exchange between paired homologs occurs in the context of a tripartite proteinaceous structure called the synaptonemal complex (SC). Genetic analyses have revealed that the genes encoding SC proteins are vital for meiotic chromosome pairing and recombination. However, the number, nature and...

journal_title：Nucleic acids research

authors： Anuradha S,Muniyappa K

更新日期：2004-04-28 00:00:00

abstract：:Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90% decrease of tufB ...

journal_title：Nucleic acids research

authors： van Delft JH,Mariñon B,Schmidt DS,Bosch L

更新日期：1987-11-25 00:00:00

abstract：:High-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP, also called CLIP-Seq) has been used to map global RNA-protein interactions. However, a critical caveat of HITS-CLIP results is that they contain non-linear background noise-different extent of non-specific interactions caused b...

journal_title：Nucleic acids research

authors： Park S,Ahn SH,Cho ES,Cho YK,Jang ES,Chi SW

更新日期：2018-11-30 00:00:00

abstract：:Large-scale automatic annotation of protein sequences remains challenging in postgenomics era. E1DS is designed for annotating enzyme sequences based on a repository of 1D signatures. The employed sequence signatures are derived using a novel pattern mining approach that discovers long motifs consisted of several sequ...

journal_title：Nucleic acids research

authors： Chien TY,Chang DT,Chen CY,Weng YZ,Hsu CM

更新日期：2008-07-01 00:00:00

abstract：:A long-range physical map of the centromeric region of human chromosome 7 has been constructed in order to define the region containing sequences with potential involvement in centromere function. The map is centered around alpha satellite DNA, a family of tandemly repeated DNA forming arrays of hundreds to thousands ...

journal_title：Nucleic acids research

authors： Wevrick R,Willard HF

更新日期：1991-05-11 00:00:00

abstract：:The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic euryarchaeon Thermococcus aggregans. This motif plays a critical role in the balance between the synthesis and degradation of the DNA chain. Five different mutations of th...

journal_title：Nucleic acids research

authors： Böhlke K,Pisani FM,Vorgias CE,Frey B,Sobek H,Rossi M,Antranikian G

更新日期：2000-10-15 00:00:00

abstract：:The MIPS Fusarium graminearum Genome Database (FGDB) is a comprehensive genome database on one of the most devastating fungal plant pathogens of wheat and barley. FGDB provides information on two gene sets independently derived by automated annotation of the F.graminearum genome sequence. A complete manually revised g...

journal_title：Nucleic acids research

authors： Güldener U,Mannhaupt G,Münsterkötter M,Haase D,Oesterheld M,Stümpflen V,Mewes HW,Adam G

更新日期：2006-01-01 00:00:00

abstract：:CDK12 (cyclin-dependent kinase 12) is a regulatory kinase with evolutionarily conserved roles in modulating transcription elongation. Recent tumor genome studies of breast and ovarian cancers highlighted recurrent CDK12 mutations, which have been shown to disrupt DNA repair in cell-based assays. In breast cancers, CDK...

journal_title：Nucleic acids research

authors： Tien JF,Mazloomian A,Cheng SG,Hughes CS,Chow CCT,Canapi LT,Oloumi A,Trigo-Gonzalez G,Bashashati A,Xu J,Chang VC,Shah SP,Aparicio S,Morin GB

更新日期：2017-06-20 00:00:00

abstract：:Homologous recombination (HR) is a highly accurate mechanism of DNA repair that can be exploited for homology-directed gene targeting. Since in most cell types HR occurs very infrequently (approximately 10(-6) to 10(-8)), its practical application has been largely restricted to specific experimental systems that allow...

journal_title：Nucleic acids research

authors： van Nierop GP,de Vries AA,Holkers M,Vrijsen KR,Gonçalves MA

更新日期：2009-09-01 00:00:00

abstract：:KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately clo...

journal_title：Nucleic acids research

authors： Shafit-Zagardo B,Maio JJ,Brown FL

更新日期：1982-05-25 00:00:00

abstract：:We have synthesized a family of double-stranded cDNAs (ds cDNAs) using as a template the family of highly purified protamine mRNAs from rainbow trout testis. Individual pure protamine cDNA components were isolated by cloning this family of protamine ds cDNAs in a plasmid vector (pMB9). Clones containing protamine sequ...

journal_title：Nucleic acids research

authors： Gedamu L,Wosnick MA,Connor W,Watson DC,Dixon GH,Iatrou K

更新日期：1981-03-25 00:00:00

abstract：:The frequencies of each of the 257 468 complete protein coding sequences (CDSs) have been compiled from the taxonomical divisions of the GenBank DNA sequence database. The sum of the codons used by 8792 organisms has also been calculated. The data files can be obtained from the anonymous ftp sites of DDBJ, Kazusa and ...

journal_title：Nucleic acids research

authors： Nakamura Y,Gojobori T,Ikemura T

更新日期：2000-01-01 00:00:00

abstract：:The DNA replication, plasmid segregation and transactivation functions of Epstein-Barr nuclear antigen 1 (EBNA1) require the binding of EBNA1 to specific DNA recognition sites in the two non-contiguous functional elements of the Epstein-Barr virus latent origin of replication, oriP . EBNA1 molecules bound to these ele...

journal_title：Nucleic acids research

authors： Avolio-Hunter TM,Frappier L

更新日期：1998-10-01 00:00:00

abstract：:The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and tw...

journal_title：Nucleic acids research

authors： Rotheneder H,Grabner M,Wintersberger E

更新日期：1991-12-25 00:00:00

abstract：:Robust estimation of allele frequencies in pools of DNA has the potential to reduce genotyping costs and/or increase the number of individuals contributing to a study where hundreds of thousands of genetic markers need to be genotyped in very large populations sample sets, such as genome wide association studies. In o...

journal_title：Nucleic acids research

authors： Brohede J,Dunne R,McKay JD,Hannan GN

更新日期：2005-09-30 00:00:00

abstract：:An 11 S ribonucleoprotein particle was isolated from maize endosperm and shown to be functionally and structurally equivalent to the mammalian signal recognition particle. However, unlike animal cells which apparently contain a single 7 SL RNA species, maize endosperm contains a heterogeneous population of 7 SL RNA. T...

journal_title：Nucleic acids research

authors： Campos N,Palau J,Zwieb C

更新日期：1989-02-25 00:00:00

abstract：:Guanine-quadruplexes (G4) consist of non-canonical four-stranded helical arrangements of guanine-rich nucleic acid sequences. The bulky and thermodynamically stable features of G4 structures have been shown in many respects to affect normal nucleic acid metabolism. In vivo conversion of G4 structures to single-strande...

journal_title：Nucleic acids research

authors： Lattmann S,Stadler MB,Vaughn JP,Akman SA,Nagamine Y

更新日期：2011-11-01 00:00:00

abstract：:Translation initiation in eukaryotes is mediated by assembly of the eIF4F complex over the m(7)GTP cap structure at the 5'-end of mRNAs. This requires an interaction between eIF4E and eIF4G, two eIF4F subunits. The Leishmania orthologs of eIF4E are structurally diverged from their higher eukaryote counterparts, since ...

journal_title：Nucleic acids research

authors： Yoffe Y,Léger M,Zinoviev A,Zuberek J,Darzynkiewicz E,Wagner G,Shapira M

更新日期：2009-06-01 00:00:00

abstract：:We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium, allowing collaborative structural proteomics to be carried out in a distributed fashion. The core of SPINE is a laborato...

journal_title：Nucleic acids research

authors： Goh CS,Lan N,Echols N,Douglas SM,Milburn D,Bertone P,Xiao R,Ma LC,Zheng D,Wunderlich Z,Acton T,Montelione GT,Gerstein M

更新日期：2003-06-01 00:00:00

abstract：:The 0.048502 megabase (Mb), primarily double-stranded DNA of bacteriophage lambda has single-stranded, complementary termini (cohesive ends) that undergo either spontaneous intramolecular joining to form open circular DNA or spontaneous intermolecular joining to form linear, end-to-end oligomeric DNAs (concatemers); c...

journal_title：Nucleic acids research

authors： Louie D,Serwer P

更新日期：1991-06-11 00:00:00

abstract：:Procollagen alpha 2 (I) mRNA can be induced congruent to 6-fold in primary avian tendon (PAT) cells on addition of ascorbate to the culture medium. Previously, we have shown that the induction is linear after a 12 h lag and requires a total of 60-72 h to achieve maximum levels. We have now investigated in more detail ...

journal_title：Nucleic acids research

authors： Lyons BL,Schwarz RI

更新日期：1984-03-12 00:00:00

abstract：:The role of the enzyme poly(adenosine diphosphate-ribose) polymerase (PADPRP) in DNA repair at the level of the gene was investigated with human HeLa cells in which PADPRP antisense transcripts are inducible with dexamethasone. After such induction, the cellular content of PADPRP is reduced by 90%. DNA damage and its ...

journal_title：Nucleic acids research

authors： Stevnsner T,Ding R,Smulson M,Bohr VA

更新日期：1994-11-11 00:00:00

abstract：:Alternative splicing of a single pre-mRNA transcript can produce protein isoforms that promote either cell growth or death. Here we show that Ro-31-8220 (Ro), an apoptotic agent that inhibits protein kinase C and activates the c-Jun N terminal kinase, decreased the proportion of the cell growth-promoting Bcl-xL splice...

journal_title：Nucleic acids research

authors： Hai Y,Cao W,Liu G,Hong SP,Elela SA,Klinck R,Chu J,Xie J

更新日期：2008-06-01 00:00:00

abstract：:A growing body of evidence suggests that genes transcribed by RNA polymerase III exhibit multiple functions within a chromosome. While the predominant function of these genes is the synthesis of RNA molecules, certain RNA polymerase III genes also function as genomic landmarks. Transfer RNA genes are known to exhibit ...

journal_title：Nucleic acids research

authors： Simms TA,Miller EC,Buisson NP,Jambunathan N,Donze D

更新日期：2004-09-30 00:00:00

abstract：:Mutational analysis has shown that the integrity of the region in domain III of 25S rRNA that is involved in binding of ribosomal protein L25 is essential for the production of mature 25S rRNA in the yeast Saccharomyces cerevisiae. However, even structural alterations that do not noticeably affect recognition by L25, ...

journal_title：Nucleic acids research

authors： van Beekvelt CA,de Graaff-Vincent M,Faber AW,van't Riet J,Venema J,Raué HA

更新日期：2001-12-15 00:00:00

abstract：:The modified nucleoside, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine, designated as Q, and its derivative, Q*, were found in tRNA's from various organisms, including several mammalian tissues, other animals such as starfish, lingula and hagfish, and wheat germ. Q isolated from rat liver tRNA ...

journal_title：Nucleic acids research

authors： Kasai H,Kuchino Y,Nihei K,Nishimura S

更新日期：1975-10-01 00:00:00