In vitro experimental system for analysis of transcription-translation coupling.

abstract：:The development of biosensors based on DNA hybridization requires a more precise knowledge of the thermodynamics of the hybridization at a solid interface. In particular, the selectivity of hybridization can be affected by a lot of parameters such as the single-strand (ss)DNA density, the pH, the ionic strength or the...

journal_title：Nucleic acids research

authors： Meunier-Prest R,Raveau S,Finot E,Legay G,Cherkaoui-Malki M,Latruffe N

更新日期：2003-12-01 00:00:00

abstract：:X chromosome inactivation results in the cis-limited inactivation of most, but not all, genes on one of the two X chromosomes in mammalian females. The molecular basis for inactivation is unknown. In order to examine the transcriptional activity of human X-linked genes, a series of mouse-human somatic cell hybrids und...

journal_title：Nucleic acids research

authors： Brown CJ,Flenniken AM,Williams BR,Willard HF

更新日期：1990-07-25 00:00:00

abstract：:Hg-UMP-containing transcripts made from chick erythroid chromatins with E. coli RNA polymerase hybridize to chick globin cDNA. Contamination with endogenous globin RNA has been largely removed by purification on SH-agarose columns at 55 degrees C. Some endogenous globin mRNA sequences remain, probably as hybrids with ...

journal_title：Nucleic acids research

authors： Konkel DA,Ingram VM

更新日期：1978-04-01 00:00:00

abstract：:This paper describes a simple program that uses a digitizing device to enter DNA sequences directly from autoradiographs into a computer. ...

journal_title：Nucleic acids research

authors： Staden R

更新日期：1984-01-11 00:00:00

abstract：:Transcription factor binding sites (TFBSs) are short DNA sequences interacting with transcription factors (TFs), which regulate gene expression. Due to the relatively short length of such binding sites, it is largely unclear how the specificity of protein-DNA interaction is achieved. Here, we have performed a genome-w...

journal_title：Nucleic acids research

authors： Zhang C,Xuan Z,Otto S,Hover JR,McCorkle SR,Mandel G,Zhang MQ

更新日期：2006-05-02 00:00:00

abstract：:The Rat Genome Database (RGD, http://rgd.mcw.edu) was developed to provide a core resource for rat researchers combining genetic, genomic, pathway, phenotype and strain information with a focus on disease. RGD users are provided with access to structured and curated data from the molecular level through to the level o...

journal_title：Nucleic acids research

authors： Dwinell MR,Worthey EA,Shimoyama M,Bakir-Gungor B,DePons J,Laulederkind S,Lowry T,Nigram R,Petri V,Smith J,Stoddard A,Twigger SN,Jacob HJ,RGD Team.

更新日期：2009-01-01 00:00:00

abstract：:The D. melanogaster Adh gene is transcribed from two different promoters; a proximal (larval) promoter is active during late embryonic and larval stages, and a distal (adult) promoter is active primarily in third instar larvae and in adult flies (1). Genetic analyses suggest that several species of the mulleri subgrou...

journal_title：Nucleic acids research

authors： Fischer JA,Maniatis T

更新日期：1985-10-11 00:00:00

abstract：:DNA replication of phi29 and related phages takes place via a strand displacement mechanism, a process that generates large amounts of single-stranded DNA (ssDNA). Consequently, phage-encoded ssDNA-binding proteins (SSBs) are essential proteins during phage phi29-like DNA replication. In the present work we analyze th...

journal_title：Nucleic acids research

authors： Gascón I,Lázaro JM,Salas M

更新日期：2000-05-15 00:00:00

abstract：:RegulonDB, first published 20 years ago, is a comprehensive electronic resource about regulation of transcription initiation of Escherichia coli K-12 with decades of knowledge from classic molecular biology experiments, and recently also from high-throughput genomic methodologies. We curated the literature to keep Reg...

journal_title：Nucleic acids research

authors： Santos-Zavaleta A,Salgado H,Gama-Castro S,Sánchez-Pérez M,Gómez-Romero L,Ledezma-Tejeida D,García-Sotelo JS,Alquicira-Hernández K,Muñiz-Rascado LJ,Peña-Loredo P,Ishida-Gutiérrez C,Velázquez-Ramírez DA,Del Moral-Chávez V,Bonavid

更新日期：2019-01-08 00:00:00

abstract：:It is believed that depending on the thermodynamic stability of the 5'-strand and the 3'-strand in the stem-loop structure of a precursor microRNA (pre-miRNA), cells preferentially select the less stable one (called the miRNA or guide strand) and destroy the other one (called the miRNA* or passenger strand). However, ...

journal_title：Nucleic acids research

authors： Ro S,Park C,Young D,Sanders KM,Yan W

更新日期：2007-01-01 00:00:00

abstract：:In eukaryotes, the tRNA-mimicking polypeptide-chain release factor, eRF1, decodes stop codons on the ribosome in a complex with eRF3; this complex exhibits striking structural similarity to the tRNA-eEF1A-GTP complex. Although amino acid residues or motifs of eRF1 that are critical for stop codon discrimination have b...

journal_title：Nucleic acids research

authors： Saito K,Ito K

更新日期：2015-05-19 00:00:00

abstract：:Restriction fragments containing established curved regions, such as the pBR322 tn3 region, the phage lambda attP region and the SV40 T-antigen and terminus of replication regions, exhibit systematically retarded elution upon anion exchange based HPLC. Using this method, we were able to detect readily other SV40 curve...

journal_title：Nucleic acids research

authors： Fack F,Sarantoglou V

更新日期：1991-08-11 00:00:00

abstract：:During culture, a chicken B cell line DT40 spontaneously mutates immunoglobulin (Ig) genes by gene conversion, which involves activation-induced cytidine deaminase (AID)-dependent homologous recombination of the variable (V) region gene with upstream pseudo-V genes. To explore whether this mutation mechanism can targe...

journal_title：Nucleic acids research

authors： Kanayama N,Todo K,Takahashi S,Magari M,Ohmori H

更新日期：2006-01-18 00:00:00

abstract：:Recent studies employing genome-wide approaches have provided an unprecedented view of the scope of L1 activities on structural variations in the human genome, and further reinforced the role of L1s as one of the major driving forces behind human genome evolution. The rapid identification of novel L1 elements by these...

journal_title：Nucleic acids research

authors： Xie Y,Rosser JM,Thompson TL,Boeke JD,An W

更新日期：2011-02-01 00:00:00

abstract：:5' Terminal cap structures of hnRNA have been characterized and the extent of capping determined as a function of embryonic development. Sea urchin embryo hnRNA contains only the type-1 cap, m7GpppNmpNp, with the type-2 cap, which has a 2'-0-methylated subpenultimate nucleotide, being associated only with stable small...

journal_title：Nucleic acids research

authors： Nemer M,Surrey S,Ginzburg I,Echols MM

更新日期：1979-01-01 00:00:00

abstract：:TFIIIC is a RNA polymerase (pol) III-specific DNA-binding factor that is required for transcription of tRNA and 5S rRNA genes. Active human TFIIIC consists of five subunits. However, an inactive form has also been isolated that lacks one of the five subunits, called TFIIIC110. A model was proposed in which pol III tra...

journal_title：Nucleic acids research

authors： Innes F,Ramsbottom B,White RJ

更新日期：2006-07-05 00:00:00

abstract：:Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast ...

journal_title：Nucleic acids research

authors： Habu T,Taki T,West A,Nishimune Y,Morita T

更新日期：1996-02-01 00:00:00

abstract：:A suppressor tRNA(Tyr) and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-L-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNA(Tyr) gene was mutated, at three positions in the D arm, to generate the internal promoter for expression. However, this tRN...

journal_title：Nucleic acids research

authors： Sakamoto K,Hayashi A,Sakamoto A,Kiga D,Nakayama H,Soma A,Kobayashi T,Kitabatake M,Takio K,Saito K,Shirouzu M,Hirao I,Yokoyama S

更新日期：2002-11-01 00:00:00

abstract：:Augmented biological activity in vitro has been demonstrated in oligonucleotides (oligos) modified to provide nuclease resistance, to enhance cellular uptake or to increase target affinity. How chemical modification affects the duration of effect of an oligo with potent activity has not been investigated directly. We ...

journal_title：Nucleic acids research

authors： Tam RC,Li Y,Noonberg S,Hwang DG,Lui G,Hunt CA,Garovoy MR

更新日期：1994-03-25 00:00:00

abstract：:CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expresse...

journal_title：Nucleic acids research

authors： Hokii Y,Sasano Y,Sato M,Sakamoto H,Sakata K,Shingai R,Taneda A,Oka S,Himeno H,Muto A,Fujiwara T,Ushida C

更新日期：2010-09-01 00:00:00

abstract：:Accumulating evidence has demonstrated that transcriptional regulation is affected by DNA methylation. Understanding the perturbation of DNA methylation-mediated regulation between transcriptional factors (TFs) and targets is crucial for human diseases. However, the global landscape of DNA methylation-mediated transcr...

journal_title：Nucleic acids research

authors： Wang Z,Yin J,Zhou W,Bai J,Xie Y,Xu K,Zheng X,Xiao J,Zhou L,Qi X,Li Y,Li X,Xu J

更新日期：2020-03-18 00:00:00

abstract：:The Chinese hamster cell line, DC-3F, is heterozygous at the DHFR locus, and each allele can be distinguished on the basis of a unique DNA restriction pattern, protein isoelectric profile and in the abundancy of the DHFR mRNAs it expresses. Although each allele produces four transcripts, 1000, 1650 and 2150 nucleotide...

journal_title：Nucleic acids research

authors： Scotto KW,Yang H,Davide JP,Melera PW

更新日期：1992-12-25 00:00:00

abstract：:KpnI restriction of DNAs from all anthropoid primates studied releases a conspicuous series of segments representing families of long, interspersed repetitive DNAs termed here the KpnI 1.2, 1.5, 1.8 and 1.9 kb families. Human KpnI 1.2 to 1.9 kb segments representative of these families were isolated and separately clo...

journal_title：Nucleic acids research

authors： Shafit-Zagardo B,Maio JJ,Brown FL

更新日期：1982-05-25 00:00:00

abstract：:Single-walled carbon nanotubes (SWNTs) have been considered as the leading candidate for nanodevice applications ranging from gene therapy and novel drug delivery to membrane separations. The miniaturization of DNA-nanotube devices for biological applications requires fully understanding DNA-nanotube interaction mecha...

journal_title：Nucleic acids research

authors： Li X,Peng Y,Qu X

更新日期：2006-08-02 00:00:00

abstract：:The HIV RT and Protease Sequence Database is an online relational database that catalogs evolutionary and drug-related human immunodeficiency virus (HIV) reverse transcriptase (RT) and protease sequence variation (http://hivdb.stanford.edu). The database contains a compilation of nearly all published HIV RT and protea...

journal_title：Nucleic acids research

authors： Shafer RW,Jung DR,Betts BJ,Xi Y,Gonzales MJ

更新日期：2000-01-01 00:00:00

abstract：:Mutations of the KRAS proto-oncogene are associated with several tumor types, which is why it is being considered as a target for anti-cancer drug development. The human KRAS promoter contains a nuclease hypersensitive element (NHE), which can bind to nuclear proteins and is believed to form G-quadruplex structures. P...

journal_title：Nucleic acids research

authors： Podbevšek P,Plavec J

更新日期：2016-01-29 00:00:00

abstract：:Adenine, thymine and cytosine PNA monomers have been prepared using 3-amino-1,2-propanediol as a starting material. The benzoyl group was used to protect the exocyclic amines of the heterocyclic bases of A and C PNA monomers and the backbone primary amine was protected with the monomethoxytrityl group. The thymine and...

journal_title：Nucleic acids research

authors： Finn PJ,Gibson NJ,Fallon R,Hamilton A,Brown T

更新日期：1996-09-01 00:00:00

abstract：:A minor tRNA-Glu1 constituent of a preparation of E. coli B tRNA-Glu (Oak Ridge) has a guanosine residue at position 66 rather than an adenosine as in the tRNA-Glu2 described by Ohashi et al. (3). Automated sequential degradation was used to sequence this region. ...

journal_title：Nucleic acids research

authors： Uziel M,Weinberger AJ

更新日期：1975-04-01 00:00:00

abstract：:I-motif DNAs have been widely employed as robust modulating components to construct reconfigurable DNA nanodevices that function well in acidic cellular environments. However, they generally display poor interactivity with fluorescent ligands under these complex conditions, illustrating a major difficulty in utilizing...

journal_title：Nucleic acids research

authors： Shi L,Peng P,Zheng J,Wang Q,Tian Z,Wang H,Li T

更新日期：2020-02-28 00:00:00

abstract：:Long non-coding RNAs (lncRNAs) that have no protein-coding capacity make up a large proportion of the transcriptome of various species. Many lncRNAs are expressed within the animal central nervous system in spatial- and temporal-specific patterns, indicating that lncRNAs play important roles in cellular processes, neu...

journal_title：Nucleic acids research

authors： Li M,Wen S,Guo X,Bai B,Gong Z,Liu X,Wang Y,Zhou Y,Chen X,Liu L,Chen R

更新日期：2012-12-01 00:00:00