X chromosome inactivation of the human TIMP gene.

abstract：:Protein structures often show similarities to another which would not be seen at the sequence level. Given the coordinates of a protein chain, the SALAMI server at www.zbh.uni-hamburg.de/salami will search the protein data bank and return a set of similar structures without using sequence information. The results page...

journal_title：Nucleic acids research

authors： Margraf T,Schenk G,Torda AE

更新日期：2009-07-01 00:00:00

abstract：:The bHLH transcription factor MyoD, the prototypical master regulator of differentiation, directs a complex program of gene expression during skeletal myogenesis. The up-regulation of the cdk inhibitor p57kip2 plays a critical role in coordinating differentiation and growth arrest during muscle development, as well as...

journal_title：Nucleic acids research

authors： Busanello A,Battistelli C,Carbone M,Mostocotto C,Maione R

更新日期：2012-09-01 00:00:00

abstract：:Cdc13 is an essential protein from Saccharomyces cerevisiae that caps telomeres by protecting the C-rich telomeric DNA strand from degradation and facilitates telomeric DNA replication by telomerase. In vitro, Cdc13 binds TG-rich single-stranded telomeric DNA with high affinity and specificity. A previously identified...

journal_title：Nucleic acids research

authors： Anderson EM,Halsey WA,Wuttke DS

更新日期：2002-10-01 00:00:00

abstract：:The nucleotide sequence for human argininosuccinate synthetase cDNA was determined by analysis of six clones isolated from a single experiment. The sequence covered 1623 nucleotides including 76 bases of poly(A) and contained a 1236 nucleotide open reading frame encoding a protein of 46,434 daltons. In one cDNA isolat...

journal_title：Nucleic acids research

authors： Bock HG,Su TS,O'Brien WE,Beaudet AL

更新日期：1983-09-24 00:00:00

abstract：:Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, ...

journal_title：Nucleic acids research

authors： Miyoshi K,Arentzen R,Huang T,Itakura K

更新日期：1980-11-25 00:00:00

abstract：:We describe the unique features of an aberrantly rearranged mu immunoglobulin heavy chain gene isolated from MPC-11 cells (a gamma 2b producing Balb/c plasmacytoma). A novel rearrangement has occurred 1.5 Kb 5' of the MPC-11 mu gene (denoted 18b mu) resulting in the deletion of the majority of the repetitive switch re...

journal_title：Nucleic acids research

authors： Greenberg R,Lang RB,Diamond MS,Marcu KB

更新日期：1982-12-11 00:00:00

abstract：:We previously reported the isolation of PRP28, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo. Sequence analysis revealed that PRP28 is included in the 'DEAD-box' gene family, members of which are thought to function as ATP-dependent RNA helicases. Gen...

journal_title：Nucleic acids research

authors： Strauss EJ,Guthrie C

更新日期：1994-08-11 00:00:00

abstract：:During mitosis the chromatin undergoes dramatic architectural changes with the halting of the transcriptional processes and evacuation of nearly all transcription associated machinery from genes and promoters. Molecular bookmarking of genes during mitosis is a mechanism of faithfully transmitting cell-specific transcr...

journal_title：Nucleic acids research

authors： Arora M,Packard CZ,Banerjee T,Parvin JD

更新日期：2016-03-18 00:00:00

abstract：:Spermine-DNA complexes have been examined by small-angle and wide-angle X-ray scattering as well as by circular dichroism studies. Condensed complexes are building up below a critical ionic strength. We have found that at one and the same ionic strength condensed complexes having two different supramolecular structure...

journal_title：Nucleic acids research

authors： Becker M,Misselwitz R,Damaschun H,Damaschun G,Zirwer D

更新日期：1979-11-10 00:00:00

abstract：:A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping. PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity. In the presence of an incomplete set of dNTPs, pfu was found to be extremely discriminative in nucleotide i...

journal_title：Nucleic acids research

authors： Hu YW,Balaskas E,Kessler G,Issid C,Scully LJ,Murphy DG,Rinfret A,Giulivi A,Scalia V,Gill P

更新日期：1998-11-01 00:00:00

abstract：:Molecular beacons (MBs) have the potential to provide a powerful tool for rapid RNA detection in living cells, as well as monitoring the dynamics of RNA expression in response to external stimuli. To exploit this potential, it is necessary to distinguish true signal from background signal due to non-specific interacti...

journal_title：Nucleic acids research

authors： Rhee WJ,Bao G

更新日期：2010-05-01 00:00:00

abstract：:The structure and organisation of the human N-ras gene has been determined by analysing cDNA clones derived from the two main mRNA transcripts. One clone in particular is 4.1 Kb long and originates from the larger (4.3 Kb) message. Sequence analysis of this clone has revealed that the N-ras gene consists of seven exon...

journal_title：Nucleic acids research

authors： Hall A,Brown R

更新日期：1985-07-25 00:00:00

abstract：:The effects of the treatment of cultured human and simian cells with Cadmium (Cd), a toxic and carcinogenic metal, were first assayed on macromolecular synthesis. It was observed that DNA synthesis was inhibited by Cd concentrations considerably lower than those inhibiting protein and RNA synthesis. Because of the nec...

journal_title：Nucleic acids research

authors： Nocentini S

更新日期：1987-05-26 00:00:00

abstract：:Studies of bacterial chromosomes and plasmids indicate that their replication initiator proteins bind to origins of replication at many double-stranded sites and also at AT-rich regions where single-stranded DNA is exposed during origin opening. Single-strand binding apparently promotes origin opening by stabilizing a...

journal_title：Nucleic acids research

authors： Chatterjee S,Jha JK,Ciaccia P,Venkova T,Chattoraj DK

更新日期：2020-11-04 00:00:00

abstract：:DNA damage-induced cell cycle checkpoints serve as surveillance mechanisms to maintain genomic stability, and are regulated by ATM/ATR-mediated signaling pathways that are conserved from yeast to humans. Trypanosoma brucei, an early divergent microbial eukaryote, lacks key components of the conventional DNA damage-ind...

journal_title：Nucleic acids research

authors： Zhou Q,Pham KTM,Hu H,Kurasawa Y,Li Z

更新日期：2019-09-05 00:00:00

abstract：:The fine structure of the melting curve for the linear colE1 DNA has been obtained. To find the ColE1 DNA regions corresponding to peaks in the melting curve's fine structure, we fixed the melted DNA regions with glyoxal /12/. Electron-microscopic denaturation maps were obtained for nine temperature points within the ...

journal_title：Nucleic acids research

authors： Borovik AS,Kalambet YA,Lyubchenko YL,Shitov VT,Golovanov EI

更新日期：1980-09-25 00:00:00

abstract：:Transcription of ribosomal RNA genes is generally accepted to correlate with cell growth. Using primary cultures of adult bovine aortic endothelial (ABAE) cells, we have shown that transcription of rDNA in confluent cells falls to 5% of the transcription level in growing cells. Protein kinase NII appears to be a limit...

journal_title：Nucleic acids research

authors： Belenguer P,Baldin V,Mathieu C,Prats H,Bensaid M,Bouche G,Amalric F

更新日期：1989-08-25 00:00:00

abstract：:A cDNA clone named 1A11 was isolated in a screen for genes that are activated by both mesoderm inducing factors FGF and activin in animal explants of Xenopus laevis embryos. In undisturbed embryos, 1A11 is expressed during the gastrula stage in the entire marginal zone where mesoderm originates, and later in the somit...

journal_title：Nucleic acids research

authors： Greene JM,Otani H,Good PJ,Dawid IB

更新日期：1993-05-25 00:00:00

abstract：:Scanning force microscopy was used to examine DNA condensates prepared with varying stoichiometries of lipospermine or polyethylenimine in physiological solution. For the first time, individual DNA strands were clearly visualized in incomplete condensates without drying. Using lipospermine at sub-saturating concentrat...

journal_title：Nucleic acids research

authors： Dunlap DD,Maggi A,Soria MR,Monaco L

更新日期：1997-08-01 00:00:00

abstract：:Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...

journal_title：Nucleic acids research

authors： Koropatnick J,Andrews G,Duerksen JD,Varshney U,Gedamu L

更新日期：1983-05-25 00:00:00

abstract：:The Network of Cancer Genes (NCG) collects and integrates data on 736 human genes that are mutated in various types of cancer. For each gene, NCG provides information on duplicability, orthology, evolutionary appearance and topological properties of the encoded protein in a comprehensive version of the human protein-p...

journal_title：Nucleic acids research

authors： Syed AS,D'Antonio M,Ciccarelli FD

更新日期：2010-01-01 00:00:00

abstract：:The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA...

journal_title：Nucleic acids research

authors： Andersen FF,Knudsen B,Oliveira CL,Frøhlich RF,Krüger D,Bungert J,Agbandje-McKenna M,McKenna R,Juul S,Veigaard C,Koch J,Rubinstein JL,Guldbrandtsen B,Hede MS,Karlsson G,Andersen AH,Pedersen JS,Knudsen BR

更新日期：2008-03-01 00:00:00

abstract：:When B cells differentiate into plasma cells, there is a large increase in the cellular content of mRNA coding for immunoglobulin. This increase cannot be fully accounted for by the increase in rate of transcription of the genes. We have investigated the possibility that the half-life of mu heavy chain mRNA increases ...

journal_title：Nucleic acids research

authors： Cox A,Emtage JS

更新日期：1989-12-25 00:00:00

abstract：:The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in...

journal_title：Nucleic acids research

authors： Hansen-Hagge TE,Trefzer U,zu Reventlow AS,Kaltoft K,Sterry W

更新日期：2001-02-15 00:00:00

abstract：:Co-option of cis-regulatory modules has been suggested as a mechanism for the evolution of expression sites during development. However, the extent and mechanisms involved in mobilization of cis-regulatory modules remains elusive. To trace the history of non-coding elements, which may represent candidate ancestral cis...

journal_title：Nucleic acids research

authors： Sanges R,Hadzhiev Y,Gueroult-Bellone M,Roure A,Ferg M,Meola N,Amore G,Basu S,Brown ER,De Simone M,Petrera F,Licastro D,Strähle U,Banfi S,Lemaire P,Birney E,Müller F,Stupka E

更新日期：2013-04-01 00:00:00

abstract：:Electrospray mass spectrometry was used to investigate the mechanism of tetramolecular G-quadruplex formation by the DNA oligonucleotide dTG(5)T, in ammonium acetate. The intermediates and products were separated according to their mass (number of strands and inner cations) and quantified. The study of the temporal ev...

journal_title：Nucleic acids research

authors： Rosu F,Gabelica V,Poncelet H,De Pauw E

更新日期：2010-08-01 00:00:00

abstract：:Sequencing of RNAs (RNA-Seq) has revolutionized the field of transcriptomics, but the reads obtained often contain errors. Read error correction can have a large impact on our ability to accurately assemble transcripts. This is especially true for de novo transcriptome analysis, where a reference genome is not availab...

journal_title：Nucleic acids research

authors： Le HS,Schulz MH,McCauley BM,Hinman VF,Bar-Joseph Z

更新日期：2013-05-01 00:00:00

abstract：:Hybridization intensities of 30 distinct short duplex DNAs measured on spotted microarrays, were directly compared with thermodynamic stabilities measured in solution. DNA sequences were designed to promote formation of perfect match, or hybrid duplexes containing tandem mismatches. Thermodynamic parameters DeltaH deg...

journal_title：Nucleic acids research

authors： Fish DJ,Horne MT,Brewood GP,Goodarzi JP,Alemayehu S,Bhandiwad A,Searles RP,Benight AS

更新日期：2007-01-01 00:00:00

abstract：:The extent of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs was determined by infrared spectroscopy. From the infrared spectra taken at 20 degrees and 52 degrees C it is concluded that E. coli and B. stearothermophlius 5S RNAs possess a large number of base pairs (Table I). Comparison of our...

journal_title：Nucleic acids research

authors： Appel B,Erdmann VA,Stulz J,Ackerman T

更新日期：1979-10-25 00:00:00

abstract：:Population-scale sequencing is increasingly uncovering large numbers of rare single-nucleotide variants (SNVs) in coding regions of the genome. The rarity of these variants makes it challenging to evaluate their deleteriousness with conventional phenotype-genotype associations. Protein structures provide a way of addr...

journal_title：Nucleic acids research

authors： Kumar S,Clarke D,Gerstein M

更新日期：2016-12-01 00:00:00