Abstract:
:The structure and organisation of the human N-ras gene has been determined by analysing cDNA clones derived from the two main mRNA transcripts. One clone in particular is 4.1 Kb long and originates from the larger (4.3 Kb) message. Sequence analysis of this clone has revealed that the N-ras gene consists of seven exons. A second clone deriving from the smaller (2 Kb) message shows that the difference between the two transcripts is a simple extension through the termination site of the 2 Kb transcript. Using S1 analysis, two transcriptional starts have been mapped, 10 bp apart. There is no obvious TATA box in the expected promoter region of the gene, though there are 4 GGGCGG sequences surrounding the start sites. The 5' untranslated sequence contains 2 ATGs upstream of the initiation codon.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hall A,Brown Rdoi
10.1093/nar/13.14.5255subject
Has Abstractpub_date
1985-07-25 00:00:00pages
5255-68issue
14eissn
0305-1048issn
1362-4962journal_volume
13pub_type
杂志文章abstract::The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.11.2455
更新日期:1981-06-11 00:00:00
abstract::Genomic Denaturing Gradient Gel Electrophoresis (gDGGE) provides an alternative to the standard method of restriction fragment length polymorphism (RFLP) analysis for identifying polymorphic sequence variation in genomic DNA. For gDGGE, genomic DNA is cleaved by restriction enzymes, separated in a polyacrylamide gel c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.7.1475
更新日期:1991-04-11 00:00:00
abstract::Alternative splicing (AS) of pre-mRNAs is an important regulatory mechanism shaping the transcriptome. In plants, only few RNA-binding proteins are known to affect AS. Here, we show that the glycine-rich RNA-binding protein AtGRP7 influences AS in Arabidopsis thaliana. Using a high-resolution RT-PCR-based AS panel, we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks873
更新日期:2012-12-01 00:00:00
abstract::Recently, a few Na+-specific RNA-cleaving DNAzymes were reported, where nucleobases are likely to play critical roles in catalysis. The NaA43 and NaH1 DNAzymes share the same 16-nt Na+-binding motif, but differ in one or two nucleotides in a small catalytic loop. Nevertheless, they display an opposite pH-dependency, i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz578
更新日期:2019-09-05 00:00:00
abstract::Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg013
更新日期:2003-01-01 00:00:00
abstract::We have examined the expression and structure of several genes belonging to two classes of vegetative specific genes of the simple eukaryote, Dictyostelium discoideum. In amebae grown on bacteria, deactivation of all vegetative specific genes occurred at the onset of development and very little mRNA exists by 8 to 10 ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.23.9679
更新日期:1989-12-11 00:00:00
abstract::The synthesis of stable RNA in Mycoplasma capricolum was studied by [32P] labeling of cellular RNA of cells grown in a partially-defined medium in the presence or absence of an amino acid mixture supplement. The results indicate that M. capricolum employs the same stringent control mechanism used by E. coli cells, as ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.15.3641
更新日期:1981-08-11 00:00:00
abstract::The model mRNA (MR), 11-mer RNA containing two nitroxide spin labels at the 5'- and 3'-terminal nucleotides and prone to form a stable homodimer (MR)2, was used for Electron Paramagnetic Resonance study of structural rearrangements in mRNA occurring upon its binding to human 80S ribosomes. The formation of two differe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1136
更新日期:2018-01-25 00:00:00
abstract::An enzyme capable of liberating functional tRNA(Lys) from Escherichia coli diacetyl-lysyl-tRNA(Lys) was purified from the archae Sulfolobus solfataricus. Contrasting with the specificity of peptidyl- tRNA hydrolase (PTH) from E.coli, the S.solfataricus enzyme readily accepts E.coli formyl-methionyl-tRNA(fMet) as a sub...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg428
更新日期:2003-06-15 00:00:00
abstract::N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.2.733
更新日期:1979-02-01 00:00:00
abstract::Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.11.3969
更新日期:1985-06-11 00:00:00
abstract::The sequencing of the full transcriptome (RNA-seq) has become the preferred choice for the measurement of genome-wide gene expression. Despite its widespread use, challenges remain in RNA-seq data analysis. One often-overlooked aspect is normalization. Despite the fact that a variety of factors or 'batch effects' can ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv736
更新日期:2015-09-18 00:00:00
abstract::The genome stability is maintained by coordinated action of DNA repairs and checkpoints, which delay progression through the cell cycle in response to DNA damage. Rad9 is conserved from yeast to human and functions in cell cycle checkpoint controls. Here, a regulatory mechanism for Rad9 function is reported. In this s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1264
更新日期:2011-06-01 00:00:00
abstract::IntAct is an open source database and software suite for modeling, storing and analyzing molecular interaction data. The data available in the database originates entirely from published literature and is manually annotated by expert biologists to a high level of detail, including experimental methods, conditions and ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl958
更新日期:2007-01-01 00:00:00
abstract::DNA damage tolerance (DDT) is responsible for genomic stability and cell viability by bypassing the replication block. In Saccharomyces cerevisiae DDT employs two parallel branch pathways to bypass the DNA lesion, namely translesion DNA synthesis (TLS) and error-free lesion bypass, which are mediated by sequential mod...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw183
更新日期:2016-06-20 00:00:00
abstract::The U1 snRNP-specific protein C contains an N-terminal zinc finger-like CH motif which is required for the binding of the U1C protein to the U1 snRNP particle. Recently a similar motif was reported to be essential for in vivo homodimerization of the yeast splicing factor PRP9. In the present study we demonstrate that ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.23.4864
更新日期:1995-12-11 00:00:00
abstract::Type IV effectors (T4Es) are proteins produced by pathogenic bacteria to manipulate host cell gene expression and processes, divert the cell machinery for their own profit and circumvent the immune responses. T4Es have been characterized for some bacteria but many remain to be discovered. To help biologists identify p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt718
更新日期:2013-11-01 00:00:00
abstract::The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multiple aspects of mitochondrial RNA metabolism. Here, we investigate the role of TbDSS-1 in RNA processing and surveillance by analyzing 12S rRNA processing intermediates in TbDSS-1 RNAi cells. RNA fragments corresponding to leader sequence upst...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm690
更新日期:2008-01-01 00:00:00
abstract::The intron 2 of the murine thymidine kinase (TK) gene was observed to contain two DNase hypersensitive site. In vitro footprinting experiments indicated specific binding sites for nuclear proteins which were characterized within the sequence of intron 2. Two GC boxes (binding sites for transcription factor SP1) and tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.24.6805
更新日期:1991-12-25 00:00:00
abstract::The RNA sequences boxA, boxB and boxC constitute the nut regions of phage lambda. They nucleate the formation of a termination-resistant RNA polymerase complex on the lambda chromosome. The complex includes E. coli proteins NusA, NusB, NusG and NusE, and the lambda N protein. A complex that includes the Nus proteins a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp452
更新日期:2009-08-01 00:00:00
abstract::The major immediate early regulatory region of human cytomegalovirus (HCMV) has a complex set of DNA sites through which both cellular and viral factors coordinately regulate immediate early gene expression. In undifferentiated human teratocarcinoma (T2) cells we have previously shown that major immediate early gene e...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.8.1767
更新日期:1991-04-25 00:00:00
abstract::Immunoglobulin (Ig) diversification by somatic hypermutation in germinal center B cells is instrumental for maturation of the humoral immune response, but also bears the risk of excessive or aberrant genetic changes. Thus, introduction of DNA damage by activation-induced cytidine deaminase as well as DNA repair by mul...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1378
更新日期:2014-04-01 00:00:00
abstract::Transactivation by the ETS family of transcription factors, whose members share structurally conserved DNA-binding domains, is variably sensitive to methylation of their target genes. The mechanism by which DNA methylation controls ETS proteins remains poorly understood. Uncertainly also pervades the effects of hemi-m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw528
更新日期:2016-10-14 00:00:00
abstract::According to the recruitment model of transcriptional activation, an activator helps initiate transcription by bringing the RNA polymerase to a specific location on the DNA through interaction with components of the transcriptional machinery. However, it is difficult to isolate and define the activities of specific ac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1227
更新日期:2010-04-01 00:00:00
abstract::The miRNA Registry provides a service for the assignment of miRNA gene names prior to publication. A comprehensive and searchable database of published miRNA sequences is accessible via a web interface (http://www.sanger.ac.uk/Software/Rfam/mirna/), and all sequence and annotation data are freely available for downloa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh023
更新日期:2004-01-01 00:00:00
abstract::We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.10.e45
更新日期:2002-05-15 00:00:00
abstract::Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5' flaps. It is also involved in the maintenance of telomeres. DNA2 is a gene conserved in eukaryotes, and a putative human ortholog of yeast DNA2 (ScDNA2) ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl070
更新日期:2006-04-04 00:00:00
abstract::We have sequenced 14 introns from the ciliate Tetrahymena thermophila and include these in an analysis of the 27 intron sequences available from seven T. thermophila protein-encoding genes. Consensus 5' and 3' splice junctions were determined and found to resemble the junctions of other nuclear pre-mRNA introns. Uniqu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.17.5133
更新日期:1990-09-11 00:00:00
abstract::Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing tran...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.4.e12
更新日期:2000-02-15 00:00:00
abstract::Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crys...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku229
更新日期:2014-05-01 00:00:00