Abstract:
:Genomic Denaturing Gradient Gel Electrophoresis (gDGGE) provides an alternative to the standard method of restriction fragment length polymorphism (RFLP) analysis for identifying polymorphic sequence variation in genomic DNA. For gDGGE, genomic DNA is cleaved by restriction enzymes, separated in a polyacrylamide gel containing a gradient of DNA denaturants, and then transferred by electroblotting to nylon membranes. Unlike other applications of DGGE, gDGGE is not limited by the size of the probe and does not require probe sequence information. gDGGE can be used in conjunction with any unique DNA probe. Here we use gDGGE with probes from the proximal region of the long arm of human chromosome 21 to identify polymorphic DNA sequence variation in this segment of the chromosome. Our screening panel consisted of DNA from nine individuals, which was cleaved with five restriction enzymes and submitted to electrophoresis in two denaturing gradient conditions. We detected at least one potential polymorphism for nine of eleven probes that were tested. Two polymorphisms, one at D21S4 and one at D21S90, were characterized in detail. Our study demonstrates that gDGGE is a fast and efficient method for identifying polymorphisms that are useful for genetic linkage analysis.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Burmeister M,diSibio G,Cox DR,Myers RMdoi
10.1093/nar/19.7.1475subject
Has Abstractpub_date
1991-04-11 00:00:00pages
1475-81issue
7eissn
0305-1048issn
1362-4962journal_volume
19pub_type
杂志文章abstract::Base specificity in the interaction of ethidium with double stranded synthetic RNA homopolymers has been studied by means of spectroscopic (UV-visible absorption and fluorescence), microcalorimetric and dilatometric techniques. The results show a strong base specificity in this interaction, the association constant wi...
journal_title:Nucleic acids research
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abstract::The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between -318 and -300 and a proximal sequence element (PSE) centered at -55. Removal or alte...
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1997-09-15 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2016-09-30 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkq1239
更新日期:2011-04-01 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkl254
更新日期:2006-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn232
更新日期:2008-06-01 00:00:00
abstract::Molecular dynamics simulations were performed on the duplex DNA dodecamers d(CGCGAA TT CGCG): d(CGCGAATTCGCG) and d(GCACGAA TT AAG): d(CTTAATTCGTGC), where TT denotes a cis, syn cyclobutane thymine dimer. The constant temperature and pressure algorithm of the AMBER 4.1 molecular-modeling package was used with explicit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.7.1432
更新日期:1997-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1031
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.16.6603
更新日期:1984-08-24 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkg310
更新日期:2003-04-15 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-07-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2002-08-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2018-01-04 00:00:00
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更新日期:1979-09-25 00:00:00
abstract::Reverse transcription of HIV-1 RNA is primed by a tRNA3(Lys) molecule bound at the primer binding site (PBS). Complex intermolecular interactions were proposed between tRNA3(Lys) and the RNA of the HIV-1 Mal isolate. Recently, an alternative interaction was proposed between the TPsiC stem of tRNA3(Lys) and a primer ac...
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更新日期:1982-07-10 00:00:00
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journal_title:Nucleic acids research
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