Abstract:
:The U1 snRNP-specific protein C contains an N-terminal zinc finger-like CH motif which is required for the binding of the U1C protein to the U1 snRNP particle. Recently a similar motif was reported to be essential for in vivo homodimerization of the yeast splicing factor PRP9. In the present study we demonstrate that the human U1C protein is able to form homodimers as well. U1C homodimers are found when (i) the human U1C protein is expressed in Escherichia coli, (ii) immunoprecipitations with anti-U1C antibodies are performed on in vitro translated U1C, and when (iii) the yeast two hybrid system is used. Analyses of mutant U1C proteins in an in vitro dimerization assay and the yeast two hybrid system revealed that amino acids within the CH motif, i.e. between positions 22 and 30, are required for homodimerization.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Gunnewiek JM,van Aarssen Y,Wassenaar R,Legrain P,van Venrooij WJ,Nelissen RLdoi
10.1093/nar/23.23.4864subject
Has Abstractpub_date
1995-12-11 00:00:00pages
4864-71issue
23eissn
0305-1048issn
1362-4962pii
5b0215journal_volume
23pub_type
杂志文章abstract::Allostery is comprehensively studied for natural macromolecules, such as proteins and nucleic acids. Here, we present controllable allostery of synthetic DNA nanostructure-enzyme systems. Rational designs of the synthetic allosteric systems are based on an in-depth understanding of allosteric sites with several types ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa488
更新日期:2020-07-27 00:00:00
abstract::X chromosome inactivation results in the cis-limited inactivation of most, but not all, genes on one of the two X chromosomes in mammalian females. The molecular basis for inactivation is unknown. In order to examine the transcriptional activity of human X-linked genes, a series of mouse-human somatic cell hybrids und...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1990-07-25 00:00:00
abstract::We have identified cDNAs encoding Mel-N1, the mouse homologue of a human nervous system-specific RNA-binding protein, Hel-N1. Two major mRNA transcripts of Mel-N1 were detected predominantly in the adult mouse brain by Northern blot analysis. To gain insight into the RNA binding specificity of Mel-N1, we performed ite...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx190
更新日期:2017-05-05 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki488
更新日期:2005-07-01 00:00:00
abstract::eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells to identify mRNAs i...
journal_title:Nucleic acids research
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更新日期:2010-12-01 00:00:00
abstract::Bacterial small RNAs (sRNAs) are a heterogeneous group of post-transcriptional regulators that often act at the heart of large networks. Hundreds of sRNAs have been discovered by genome-wide screens and most of these sRNAs exert their functions by base-pairing with target mRNAs. However, studies addressing the molecul...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-11-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp415
更新日期:2009-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-06-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.5.1155
更新日期:1993-03-11 00:00:00
abstract::Methylation of CpG motifs in DNA is involved in the control of gene expression and in several other epigenic effects. It suppresses also the immuno-stimulation properties of bacterial or viral DNAs that contain CPGS: However, effects of methylation on the DNA structure and dynamics are not clear. Here we carried out a...
journal_title:Nucleic acids research
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更新日期:2001-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl287
更新日期:2006-05-08 00:00:00
abstract::The transcriptional activity of a human transposon-like family of repeats, called the THE-1 family, has been studied in cell culture and in human tissue. Both strands of THE-1 are present in several discrete length poly A plus RNAs. Primer extension studies and the structures of cDNA clones show that these THE-1 trans...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1987-07-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-01-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1983-12-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
abstract::The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is an expertly curated database of literature-derived functional information for the model organism budding yeast, Saccharomyces cerevisiae. SGD constantly strives to synergize new types of experimental data and bioinformatics predictions with existin...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-08-15 00:00:00
abstract::The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers...
journal_title:Nucleic acids research
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更新日期:1991-07-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2012-07-01 00:00:00
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更新日期:1983-01-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-04-06 00:00:00
abstract::The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acidocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acidocaldari...
journal_title:Nucleic acids research
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更新日期:1990-03-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2018-07-02 00:00:00
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更新日期:1990-01-25 00:00:00
abstract::Various in vivo mutagenesis methods have been developed to facilitate fast and efficient continuous evolution of proteins in cells. However, they either modify the DNA region that does not match the target gene, or suffer from low mutation rates. Here, we report a mutator, eMutaT7 (enhanced MutaT7), with very fast in ...
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更新日期:2021-01-06 00:00:00