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A circular dichroism study of the structure of Penicillium chrysogenum mycovirus.

Abstract：

:We have examined the absorption and circular dichroism spectra of intact Penicillium chrysogenum virus, empty capsid particles, and isolated double-stranded RNA. The absorbance at 260 nm of intact virus was less than 4% hypochromic relative to the absorbances of the free double-stranded RNA and free viral protein, indicating very little change in the base stacking interactions of the RNA. Circular dichroism studies of intact virus indicate that the capsid protein consists of 45% alpha-helix. Empty capsids, containing a protein of the same molecular weight as intact virus protein, were found to have 30% alpha-helix, suggesting a conformational change in the capsid upon assembly with RNA. The conformation of double-stranded RNA in the virus was slightly altered from the solution structure of the RNA in 0.01 M Na+ and resembled the conformation of double-stranded RNA partially bound with spermidine. However, the virus does not appear to contain polyamines. Electrophoretic experiments indicate a pH- and salt-titratable RNA binding site on the capsid protein in virus disrupted by urea or non-ionic detergents. The results are consistent with significant ionic interactions between the RNA and the capsid protein in the virus.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Edmondson SP,Gray DM

doi

10.1093/nar/11.1.175

subject

Has Abstract

pub_date

1983-01-11 00:00:00

pages

175-92

issue

1

eissn

0305-1048

issn

1362-4962

journal_volume

11

pub_type

杂志文章

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