Abstract:
:Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cochet M,Perrin F,Gannon F,Krust A,Chambon P,McKnight GS,Lee DC,Mayo KE,Palmiter Rdoi
10.1093/nar/6.7.2435subject
Has Abstractpub_date
1979-06-11 00:00:00pages
2435-52issue
7eissn
0305-1048issn
1362-4962journal_volume
6pub_type
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