Abstract：
:The Bcl-2 protein has an anti-apoptotic effect in neuronal and other cell types. We show for the first time that the Bcl-2 promoter is activated by the neuronal survival factor nerve growth factor (NGF) and that this effect is dependent on a region of the promoter from -1472 to -1414. This activation requires the Rap-1 G protein and the MEK-1 and p42/p44 MAPK enzymes but is independent of other NGF-activated signalling pathways involving protein kinase A or protein kinase C.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Liu YZ,Boxer LM,Latchman DSdoi
10.1093/nar/27.10.2086keywords:
subject
Has Abstractpub_date
1999-05-15 00:00:00pages
2086-90issue
10eissn
0305-1048issn
1362-4962pii
gkc368journal_volume
27pub_type
杂志文章abstract：:The ability of histone chaperone Anti-silencing factor 1 (Asf1) to direct acetylation of lysine 56 of histone H3 (H3K56ac) represents an important regulatory step in genome replication and DNA repair. In Saccharomyces cerevisiae, Asf1 interacts functionally with a second chaperone, Vps75, and the lysine acetyltransfer...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz508
更新日期：2019-08-22 00:00:00
abstract：:High-throughput screening assays have been developed to rapidly identify small molecule inhibitors targeting catalytic group I introns. Biochemical reactions catalyzed by a self-splicing group I intron derived from Pneumocystis carinii or from bacteriophage T4 have been investigated. In vitro biochemical assays amenab...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.24.5051
更新日期：1996-12-15 00:00:00
abstract：:Resistance to androgen receptor (AR)-targeted therapies in prostate cancer (PC) is a major clinical problem. A key mechanism of treatment resistance in advanced PC is the generation of alternatively spliced forms of the AR termed AR variants (AR-Vs) that are refractory to targeted agents and drive tumour progression. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz286
更新日期：2019-06-20 00:00:00
abstract：:Thyroglobulin (Tg), the precursor of thyroid hormones, is a 660.000 Da dimeric glycoprotein synthesized exclusively in the thyroid gland. We have cloned the human thyroglobulin gene from cosmid and phage libraries and constructed a complete restriction map. The gene encodes an 8.7 kb mRNA, covers at least 300 kb DNA a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.13.5171
更新日期：1986-07-11 00:00:00
abstract：:The genes for mitoribosomal protein S12 (Mrps12) and mitochondrial seryl-tRNA ligase (Sarsm and Sars2) are oppositely transcribed from a conserved promoter region of <200 bp in both human and mouse. Using a dual reporter vector we identified an array of 4 CCAAT box elements required for efficient transcription of the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1037
更新日期：2007-01-01 00:00:00
abstract：:Adaptive Laboratory Evolution (ALE) has emerged as an experimental approach to discover causal mutations that confer desired phenotypic functions. ALE not only represents a controllable experimental approach to systematically discover genotype-phenotype relationships, but also allows for the revelation of the series o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky983
更新日期：2019-01-08 00:00:00
abstract：:The TPA-inducible transcription factor AP-1, consisting of homo- or hetero-dimers of members of the Jun- and Fos-families, regulates transcription of a wide variety of genes containing the TPA response element (TRE). In P19 embryonal carcinoma (EC) cells, Jun D is the only component of AP-1 expressed, while in these c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.1.125
更新日期：1992-01-11 00:00:00
abstract：:One of the obstacles hindering a better understanding of cancer is its heterogeneity. However, computational approaches to model cancer heterogeneity have lagged behind. To bridge this gap, we have developed a new probabilistic approach that models individual cancer cases as mixtures of subtypes. Our approach can be s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt577
更新日期：2013-09-01 00:00:00
abstract：:DrugCentral (http://drugcentral.org) is an open-access online drug compendium. DrugCentral integrates structure, bioactivity, regulatory, pharmacologic actions and indications for active pharmaceutical ingredients approved by FDA and other regulatory agencies. Monitoring of regulatory agencies for new drugs approvals ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw993
更新日期：2017-01-04 00:00:00
abstract：:YY1 is ubiquitously expressed zinc finger DNA binding protein. It can act as a transcriptional repressor or activator and, when binding at the initiator element, as a component of the basal transcription complex. Binding sites for YY1 have been reported in a wide variety of promoters and they exhibit substantial diver...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.21.4457
更新日期：1995-11-11 00:00:00
abstract：:We have recently described three group I introns inserted into a single gene, orf142, of the staphylococcal bacteriophage Twort and suggested the presence of at least two additional self-splicing introns in this phage genome. Here we report that two previously uncharacterized introns, 429 and 1087 nt in length, interr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.9.1935
更新日期：2002-05-01 00:00:00
abstract：:Cloned coding sequences for rat and human ornithine transcarbamylase (OTC) were obtained by screening a rat and a human cDNA library respectively with a synthetic oligonucleotide corresponding to 27 bases of the rat sequence. These clones, 1100 bp long for the rat clone and 1300 bp for the human, contain approximately...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.1.155
更新日期：1985-01-11 00:00:00
abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia (ATL). The HTLV-1 basic leucine zipper protein (HBZ) is expressed in all cases of ATL and is directly associated with virus pathogenicity. The two isoforms of the HBZ protein are synthesized from antisense messenger RNAs (mRN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky802
更新日期：2018-11-16 00:00:00
abstract：:Among four types of bacterial restriction enzymes that cleave a foreign DNA depending on its methylation status, type I enzymes composed of three subunits are interesting because of their unique DNA cleavage and translocation mechanisms performed by the restriction subunit (HsdR). The elucidated N-terminal fragment st...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp603
更新日期：2009-11-01 00:00:00
abstract：:Microsatellites are widely used as genetic markers because they are co-dominant, multiallelic, easily scored and highly polymorphic. A major drawback of microsatellite markers is the time and cost required to characterise them. We have developed a novel technique to reduce this cost by producing a microsatellite-rich ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.21.4369
更新日期：1996-11-01 00:00:00
abstract：:The allele frequency net database (http://www.allelefrequencies.net) is an online repository that contains information on the frequencies of immune genes and their corresponding alleles in different populations. The extensive variability observed in genes and alleles related to the immune system response and its signi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1128
更新日期：2011-01-01 00:00:00
abstract：:In this article, we introduce the Gypsy Database (GyDB) of mobile genetic elements, an in-progress database devoted to the non-redundant analysis and evolutionary-based classification of mobile genetic elements. In this first version, we contemplate eukaryotic Ty3/Gypsy and Retroviridae long terminal repeats (LTR) ret...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm697
更新日期：2008-01-01 00:00:00
abstract：:Upon HIV-1 infection of a target cell, the viral reverse transcriptase (RT) copies the genomic RNA to synthesize the viral DNA. The genomic RNA is within the incoming HIV-1 core where it is coated by molecules of nucleocapsid (NC) protein that chaperones the reverse transcription process. Indeed, the RT chaperoning pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr117
更新日期：2011-07-01 00:00:00
abstract：:We present a study of how substituent groups of naturally occurring and modified nucleotide bases affect the degree of hydration of right-handed B-DNA and left-handed Z-DNA. A comparison of poly(dG-dC) and poly(dG-dm5C) titrations with the lipotropic salts of the Hofmeister series infers that the methyl stabilization ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.25.5978
更新日期：1993-12-25 00:00:00
abstract：:Systems biologists aim to decipher the structure and dynamics of signaling and regulatory networks underpinning cellular responses; synthetic biologists can use this insight to alter existing networks or engineer de novo ones. Both tasks will benefit from an understanding of which structural and dynamic features of ne...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv595
更新日期：2015-10-30 00:00:00
abstract：:Synthesis and antisense activity of oligonucleotides modified with 2'-O-[2-[(N,N-dimethylamino)oxy] ethyl] (2'-O-DMAOE) are described. The 2'-O-DMAOE-modified oligonucleotides showed superior metabolic stability in mice. The phosphorothioate oligonucleotide 'gapmers', with 2'-O-DMAOE- modified nucleoside residues at t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh220
更新日期：2004-02-03 00:00:00
abstract：:Active DNA demethylation (ADDM) in mammals occurs via hydroxylation of 5-methylcytosine (5mC) by TET and/or deamination by AID/APOBEC family enzymes. The resulting 5mC derivatives are removed through the base excision repair (BER) pathway. At present, it is unclear how the cell manages to eliminate closely spaced 5mC ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw059
更新日期：2016-05-05 00:00:00
abstract：:The GeneSpeed database (http://genespeed.uchsc.edu/) is an online database and resource tool facilitating the detailed study of protein domain homology in the transcriptomes of Homo sapiens, Mus musculus, Drosophila melanogaster and Caenorhabditis elegans. The population schema for the GeneSpeed database takes advanta...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl990
更新日期：2007-01-01 00:00:00
abstract：:Arguably, the most bizarre mitochondrial DNA (mtDNA) is that of the euglenozoan eukaryote Diplonema papillatum. The genome consists of numerous small circular chromosomes none of which appears to encode a complete gene. For instance, the cox1 coding sequence is spread out over nine different chromosomes in non-overlap...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq883
更新日期：2011-02-01 00:00:00
abstract：:Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzym...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.3.679
更新日期：1978-03-01 00:00:00
abstract：:In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dual prokaryotic and e...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni055
更新日期：2005-03-22 00:00:00
abstract：:The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids in solution. The goal is to predict not just a melting temperature for a hybridized pair of nucleic acids, but entire equilibrium melting profiles as a function of temperature. The two molecules are not required to be complementa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki591
更新日期：2005-07-01 00:00:00
abstract：:Gene Ontology (GO) is the most widely accepted attempt to construct a unified and structured vocabulary for the description of genes and their products in any organism. Annotation by GO terms is performed in most of the current genome projects, which besides generality has the advantage of being very convenient for co...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg582
更新日期：2003-07-01 00:00:00
abstract：:Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (posi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq341
更新日期：2010-09-01 00:00:00
abstract：:DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restriction fragments and Maxam-Gilbert sequencing gel methods of analysis. One method utilizes the enzyme, DNase I, as the DNA cleaving agent while the other employs the synthetic molecule, methidium-propyl-EDTA (MPE). For actin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.16.5555
更新日期：1983-08-25 00:00:00
