Abstract：
:The TPA-inducible transcription factor AP-1, consisting of homo- or hetero-dimers of members of the Jun- and Fos-families, regulates transcription of a wide variety of genes containing the TPA response element (TRE). In P19 embryonal carcinoma (EC) cells, Jun D is the only component of AP-1 expressed, while in these cells until now none of the members of the jun- and fos-families have been found to be inducable by external stimuli. Here we demonstrate that Jun B is the only member of the Jun- and Fos-families that is induced by Epidermal Growth Factor (EGF) in transfected murine P19 EC cells, expressing functional human EGF receptors (hEGF-Rs). Induction of jun B can be mimicked in wild type P19 EC cells by the synergistic action of the phorbol ester TPA and the calcium ionophore A23187, through activation of signal transduction pathways, that are activated simultaneously by EGF. The EGF induced jun B expression in the hEGF-R expressing P19 EC cells is mediated by an inverted repeat (IR) sequence in the jun B promoter, previously shown to be responsive to both PKC and PKA signal transduction. Transactivation of the IR sequence by EGF can be blocked completely by prior expression of antisense Jun D, but not by antisense c-Jun. These studies therefore implicate Jun D in the regulation of immediate early gene expression by external stimuli.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
den Hertog J,de Groot RP,de Laat SW,Kruijer Wdoi
10.1093/nar/20.1.125subject
Has Abstractpub_date
1992-01-11 00:00:00pages
125-30issue
1eissn
0305-1048issn
1362-4962journal_volume
20pub_type
杂志文章abstract：:The study of pathogenic mitochondrial DNA mutations has, in most cases, relied on the production of transmitochondrial cybrids. Although the procedure to produce such cybrids is well established, it is laborious and cumbersome. Moreover, the mechanical enucleation procedure is inefficient and different techniques have...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng100
更新日期：2003-08-15 00:00:00
abstract：:In many organisms (e.g., gram-positive eubacteria) Gin-tRNA is not formed by direct glutaminylation of tRNAGln but by a specific transamidation of Glu-tRNAGln. We wondered whether a similar transamidation pathway also operates in the formation of Asn-tRNA in these organisms. Therefore we tested in S-100 preparations o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.14.2648
更新日期：1996-07-15 00:00:00
abstract：:A type II restriction endonuclease, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.16.5467
更新日期：1983-08-25 00:00:00
abstract：:TOP mRNAs are vertebrate transcripts which contain a 5'terminal oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5'TOP. In the present study we show that elongation factor 2 (EF2) mRNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.5.995
更新日期：1997-03-01 00:00:00
abstract：:Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.12.4391
更新日期：1977-12-01 00:00:00
abstract：:Nuclear extracts prepared from growth hormone-secreting (GC) and prolactin-secreting (235-1) rat anterior pituitary cell lines were compared for their ability to bind to the DNA sequences conferring tissue-specificity to the expression of the rat growth hormone (rGH) gene promoter. Cell-specific differences in the int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.17.5235
更新日期：1990-09-11 00:00:00
abstract：:Several mono-, di-, tetra-, penta- and nonaribozymes were developed. These multitarget-ribozymes were targeted to cleave HIV-1 env RNA at up to nine different conserved sites. Each multitarget-ribozyme consisted of a chain of up to nine hammerhead motifs, each flanked by a different targeting sequence. The multitarget...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.17.4581
更新日期：1992-09-11 00:00:00
abstract：:Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits transcription of m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.12.2907
更新日期：1993-06-25 00:00:00
abstract：:Constitutive expression of major histocompatibility complex class II (MHC II) antigens normally occurs in B-lymphocytes and antigen presenting cells of the monocyte/macrophage lineage. However, many malignant tumours and transformed cells express these proteins aberrantly. We demonstrate here that the MHC II DR alpha ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.18.4881
更新日期：1992-09-25 00:00:00
abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.13.4409
更新日期：1983-07-11 00:00:00
abstract：:New transcripts generated by RNA polymerase II (RNAPII) are generally processed in order to form mature mRNAs. Two key processing steps include a precise cleavage within the 3' end of the pre-mRNA, and the subsequent polymerization of adenosines to produce the poly(A) tail. In yeast, these two functions are performed ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx674
更新日期：2017-09-29 00:00:00
abstract：:We describe the first polyA-containing processed pseudogene reported in the chicken. It includes a 0.52 kb open reading frame which could encode a 175 amino acid protein. The putative protein shows extensive homology to the ras oncogene superfamily, being most closely related to the yeast protein YP2. It is one of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.5.2123
更新日期：1986-03-11 00:00:00
abstract：:The HhaI methyltransferase recognizes the sequence GCGC and transfers a methyl group to C5 of the first cytosine residue. All m5C-methyltransferases contain a highly conserved sequence motif called the P-C motif. The cysteine residue of this motif is involved in catalysis by forming a covalent bond with the 6-position...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.10.2459
更新日期：1993-05-25 00:00:00
abstract：:The ribosomal proteins L4 and L22 form part of the peptide exit tunnel in the large ribosomal subunit. In Escherichia coli, alterations in either of these proteins can confer resistance to the macrolide antibiotic, erythromycin. The structures of the 30S as well as the 50S subunits from each antibiotic resistant mutan...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh913
更新日期：2004-10-27 00:00:00
abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw348
更新日期：2016-07-08 00:00:00
abstract：:Chromatin organization and epigenetic markers influence splicing, though the magnitudes of these effects and the mechanisms are largely unknown. Here, we demonstrate that linker histone H1.5 influences mRNA splicing. We observed that linker histone H1.5 binds DNA over splice sites of short exons in human lung fibrobla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz338
更新日期：2019-07-09 00:00:00
abstract：:Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.13.2885
更新日期：1980-07-11 00:00:00
abstract：:RNA exhibits a large diversity of conformations. Three thousand nucleotides of 23S and 5S ribosomal RNA from a structure of the large ribosomal subunit were analyzed in order to classify their conformations. Fourier averaging of the six 3D distributions of torsion angles and analyses of the resulting pseudo electron m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh333
更新日期：2004-03-11 00:00:00
abstract：:SMILE (small heterodimer partner interacting leucine zipper protein) has been identified as a coregulator in ER signaling. In this study, we have examined the effects of SMILE on other NRs (nuclear receptors). SMILE inhibits GR, CAR and HNF4 alpha-mediated transactivation. Knockdown of SMILE gene expression increases ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp333
更新日期：2009-07-01 00:00:00
abstract：:Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.2.e2
更新日期：2000-01-15 00:00:00
abstract：:REV1 and DNA Polymerase ζ (REV3 and REV7) play important roles in translesion DNA synthesis (TLS) in which DNA replication bypasses blocking lesions. REV1 and Polζ have also been implicated in promoting repair of DNA double-stranded breaks (DSBs). However, the mechanism by which these two TLS polymerases increase tole...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr769
更新日期：2012-01-01 00:00:00
abstract：:G-quadruplex (G4) DNA structures can form physical barriers within the genome that must be unwound to ensure cellular genomic integrity. Here, we report unanticipated roles for the Escherichia coli Rep helicase and RecA recombinase in tolerating toxicity induced by G4-stabilizing ligands in vivo. We demonstrate that R...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa442
更新日期：2020-07-09 00:00:00
abstract：:New linker phosphoramidite reagents containing a cleavable 3'-ester linkage are used for attaching the first nucleoside to the surface of a solid- phase support. Inexpensive, underivatized amino supports, such as long chain alkylamine controlled-pore glass, can serve as universal supports. No modifications to phosphor...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh222
更新日期：2004-01-29 00:00:00
abstract：:The apolipoprotein AI (apoAI) promoter/enhancer contains multiple cis -acting elements on which a variety of hepatocyte-enriched and ubiquitous transcription factors function synergistically to regulate liver-specific transcription. Adenovirus E1A proteins repress tissue-specific gene expression and disrupt the differ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.7.1761
更新日期：1998-04-01 00:00:00
abstract：:Details of polynucleotide flexibility may be probed through a combination of semiempirical potential energy calculations and statistical mechanical analyses. The pseudorotational motions of the furanose and the long-range correlated rotations of the chain backbone are described briefly here. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.3.777
更新日期：1982-02-11 00:00:00
abstract：:A microchip method has been developed for massive and parallel thermodynamic analyses of DNA duplexes. Fluorescently labeled oligonucleotides were hybridized with oligonucleotides immobilized in the 100 x 100 x 20 mum gel pads of the microchips. The equilibrium melting curves for all microchip duplexes were measured i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.6.1515
更新日期：1998-03-15 00:00:00
abstract：:SSCP and heteroduplex analysis (HA) continue to be the most popular methods of mutation detection due to their simplicity, high sensitivity and low cost. The advantages of these methods are most clearly visible when large genes, such as BRCA1 and BRCA2, are scanned for scattered unknown mutations and/or when a large n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.14.e71
更新日期：2001-07-15 00:00:00
abstract：:Homeodomains appear to be one of the most frequently employed DNA-binding domains in a superfamily of transacting factors. It is likely that during evolution several sub-types of homeodomain have evolved from a common ancestral domain, resulting in distinct but closely related DNA-binding preferences. Here we describe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.7.1202
更新日期：1994-04-11 00:00:00
abstract：:During the formation of a transcriptionally active macronucleus, ciliated protozoa excise large numbers of interstitial segments of DNA (internal eliminated sequences; IESs) from their chromosomes. In this study we analyze the published sequences of 20 IESs that interrupt surface protein genes of Paramecium and identi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.11.2006
更新日期：1995-06-11 00:00:00
abstract：:We report the synthesis of new phosphoramidite building blocks and their use for the modification of oligonucleotides with hydrazides. The reaction of these hydrazide oligonucleotides with active esters and aldehydes is demonstrated for solution conjugation and immobilization. Compared with the established amino modif...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf594
更新日期：2002-11-01 00:00:00
