Consensus inverted terminal repeat sequence of Paramecium IESs: resemblance to termini of Tc1-related and Euplotes Tec transposons.

abstract：:RNA molecules of > 20 nucleotides have been the focus of numerous recent NMR structural studies. Several investigators have used the UNCG family of hairpins to ensure proper folding. We show that th UUCG hairpin has a minimum requirement of a two base-pair stem. Hairpins with a CG loop closing base pair and an initial...

journal_title：Nucleic acids research

authors： Molinaro M,Tinoco I Jr

更新日期：1995-08-11 00:00:00

abstract：:Here, we describe the development of WikiPathways (http://www.wikipathways.org), a public wiki for pathway curation, since it was first published in 2008. New features are discussed, as well as developments in the community of contributors. New features include a zoomable pathway viewer, support for pathway ontology a...

journal_title：Nucleic acids research

authors： Kelder T,van Iersel MP,Hanspers K,Kutmon M,Conklin BR,Evelo CT,Pico AR

更新日期：2012-01-01 00:00:00

abstract：:The DEAH-box NTPase Prp43 disassembles spliceosomes in co-operation with the cofactors Ntr1/Spp382 and Ntr2, forming the NTR complex. How Prp43 is regulated by its cofactors to discard selectively only intron-lariat spliceosomes (ILS) and defective spliceosomes and to prevent disassembly of earlier and properly assemb...

journal_title：Nucleic acids research

authors： Fourmann JB,Tauchert MJ,Ficner R,Fabrizio P,Lührmann R

更新日期：2017-04-20 00:00:00

abstract：:Many complex viruses package their genomes into empty protein shells and bacteriophages of the Cystoviridae family provide some of the simplest models for this. The cystoviral hexameric NTPase, P4, uses chemical energy to translocate single-stranded RNA genomic precursors into the procapsid. We previously dissected th...

journal_title：Nucleic acids research

authors： El Omari K,Meier C,Kainov D,Sutton G,Grimes JM,Poranen MM,Bamford DH,Tuma R,Stuart DI,Mancini EJ

更新日期：2013-11-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) terminase is composed of subunits pUL56 (130 kDa) and pUL89 ( approximately 75 kDa), encoded by the UL56 and UL89 genes. In a recent investigation, we demonstrated that the main ATPase activity is associated with the large terminase subunit pUL56. The protein has two putative ATP-binding s...

journal_title：Nucleic acids research

authors： Scholz B,Rechter S,Drach JC,Townsend LB,Bogner E

更新日期：2003-03-01 00:00:00

abstract：:We have isolated the poly(A) polymerase (PAP) encoding gene pla1 [for poly(A) polymerase] from the fission yeast Schizosaccharomyces pombe. Protein sequence alignments with other poly(A) polymerases reveal that pla1 is more closely related to Saccharomyces cerevisiae PAP than to bovine PAP. The two yeast poly(A) polym...

journal_title：Nucleic acids research

authors： Ohnacker M,Minvielle-Sebastia L,Keller W

更新日期：1996-07-01 00:00:00

abstract：:Genomic DNA methylation contributes substantively to transcriptional regulations that underlie mammalian development and cellular differentiation. Much effort has been made to decipher the molecular mechanisms governing the establishment and maintenance of DNA methylation patterns. However, little is known about genom...

journal_title：Nucleic acids research

authors： Xie H,Wang M,de Andrade A,Bonaldo Mde F,Galat V,Arndt K,Rajaram V,Goldman S,Tomita T,Soares MB

更新日期：2011-05-01 00:00:00

abstract：:In most cells, transcriptionally inactive heterochromatin is preferentially localized in the nuclear periphery and transcriptionally active euchromatin is localized in the nuclear interior. Different cell types display characteristic chromatin distribution patterns, which change dramatically during cell differentiatio...

journal_title：Nucleic acids research

authors： Bergqvist C,Niss F,Figueroa RA,Beckman M,Maksel D,Jafferali MH,Kulyté A,Ström AL,Hallberg E

更新日期：2019-05-21 00:00:00

abstract：:The antitumour antibiotics bleomycin and actinomycin are commonly used therapeutically in combination. One causes metal ion- and oxygen-dependent oxidative damage to DNA, while the other acts at the level of DNA via intercalation of its phenoxazone chromophore and probable inhibition of topoisomerases. Both drugs bind...

journal_title：Nucleic acids research

authors： Bailly C,Kénani A,Waring MJ

更新日期：1997-04-15 00:00:00

abstract：:Since July 1995, the European Bioinformatics Institute (EBI) has maintained RHdb (http://www.ebi.ac.uk/RHdb/RHdb.html ), a public database for radiation hybrid data. Radiation hybrid mapping is an important technique for determining high resolution maps. Recently, CORBA access has been added to Rhdb. The EBI is an Out...

journal_title：Nucleic acids research

authors： Lijnzaad P,Helgesen C,Rodriguez-Tomé P

更新日期：1998-01-01 00:00:00

abstract：:The U1A protein is a sequence-specific RNA binding protein found in the U1 snRNP particle where it binds to stem/loop II of U1 snRNA. U1A contains two 'RNP' or 'RRM' (RNA Recognition Motif) domains, which are common to many RNA-binding proteins. The N-terminal RRM has been shown to bind specifically to the U1 RNA stem...

journal_title：Nucleic acids research

authors： Hall KB,Stump WT

更新日期：1992-08-25 00:00:00

abstract：:An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA templa...

journal_title：Nucleic acids research

authors： Ponelies N,Stein N,Weber G

更新日期：1997-09-01 00:00:00

abstract：:Long non-coding RNAs (lncRNAs) play critical roles in diverse cellular processes; however, their involvement in many critical aspects of the immune response including the interferon (IFN) response remains poorly understood. To address this gap, we compared the global gene expression pattern of primary human hepatocyte...

journal_title：Nucleic acids research

authors： Kambara H,Niazi F,Kostadinova L,Moonka DK,Siegel CT,Post AB,Carnero E,Barriocanal M,Fortes P,Anthony DD,Valadkhan S

更新日期：2014-01-01 00:00:00

abstract：:The archaeal exosome is a phosphorolytic 3'-5' exoribonuclease complex. In a reverse reaction it synthesizes A-rich RNA tails. Its RNA-binding cap comprises the eukaryotic orthologs Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. In Sulfolobus solfataricus DnaG and Rrp4 but not Csl4 show preference f...

journal_title：Nucleic acids research

authors： Hou L,Klug G,Evguenieva-Hackenberg E

更新日期：2014-11-10 00:00:00

abstract：:The HAMSTeRS WWW site was set up in 1996 in order to facilitate easy access to, and aid understanding of, the causes of haemophilia A at the molecular level; previously, the first and second text editions of the database have been published in Nucleic Acids Research. This report describes the facilities originally ava...

journal_title：Nucleic acids research

authors： Kemball-Cook G,Tuddenham EG

更新日期：1997-01-01 00:00:00

abstract：:We report the latest release (version 3.0) of the CATH protein domain database (http://www.cathdb.info). There has been a 20% increase in the number of structural domains classified in CATH, up to 86 151 domains. Release 3.0 comprises 1110 fold groups and 2147 homologous superfamilies. To cope with the increases in di...

journal_title：Nucleic acids research

authors： Greene LH,Lewis TE,Addou S,Cuff A,Dallman T,Dibley M,Redfern O,Pearl F,Nambudiry R,Reid A,Sillitoe I,Yeats C,Thornton JM,Orengo CA

更新日期：2007-01-01 00:00:00

abstract：:The PD-(D/E)XK type II restriction endonuclease ThaI cuts the target sequence CG/CG with blunt ends. Here, we report the 1.3 Å resolution structure of the enzyme in complex with substrate DNA and a sodium or calcium ion taking the place of a catalytic magnesium ion. The structure identifies Glu54, Asp82 and Lys93 as t...

journal_title：Nucleic acids research

authors： Firczuk M,Wojciechowski M,Czapinska H,Bochtler M

更新日期：2011-01-01 00:00:00

abstract：:The ASTRAL compendium provides several databases and tools to aid in the analysis of protein structures, particularly through the use of their sequences. It is partially derived from the SCOP database of protein domains, and it includes sequences for each domain as well as other resources useful for studying these seq...

journal_title：Nucleic acids research

authors： Chandonia JM,Walker NS,Lo Conte L,Koehl P,Levitt M,Brenner SE

更新日期：2002-01-01 00:00:00

abstract：:Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...

journal_title：Nucleic acids research

authors： Kang JJ,Watson RM,Fisher ME,Higuchi R,Gelfand DH,Holland MJ

更新日期：2000-01-15 00:00:00

abstract：:Acinetobacter sp. strain ADP1 is a nutritionally versatile soil bacterium closely related to representatives of the well-characterized Pseudomonas aeruginosa and Pseudomonas putida. Unlike these bacteria, the Acinetobacter ADP1 is highly competent for natural transformation which affords extraordinary convenience for ...

journal_title：Nucleic acids research

authors： Barbe V,Vallenet D,Fonknechten N,Kreimeyer A,Oztas S,Labarre L,Cruveiller S,Robert C,Duprat S,Wincker P,Ornston LN,Weissenbach J,Marlière P,Cohen GN,Médigue C

更新日期：2004-10-28 00:00:00

abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...

journal_title：Nucleic acids research

authors： Kong Q,Maizels N

更新日期：2001-03-15 00:00:00

abstract：:We have amplified the large subunit ribosomal DNA (LSUrDNA) of the 12 described Naegleria spp. and of 34 other Naegleria lineages that might be distinct species. Two strains yielded a product that is longer than 3 kb, which is the length of the LSUrDNA of all described Naegleria spp. Sequencing data revealed that the ...

journal_title：Nucleic acids research

authors： De Jonckheere JF,Brown S

更新日期：1998-01-15 00:00:00

abstract：:For sophisticated gene targeting procedures requiring two sequential selective steps to operate efficiently it is essential that the marker genes used are not prone to position effects. The double replacement gene targeting procedure, to produce mice with subtle gene alterations, is based on the use of hypoxanthine ph...

journal_title：Nucleic acids research

authors： Melton DW,Ketchen AM,Selfridge J

更新日期：1997-10-01 00:00:00

abstract：:X chromosome inactivation results in the cis-limited inactivation of most, but not all, genes on one of the two X chromosomes in mammalian females. The molecular basis for inactivation is unknown. In order to examine the transcriptional activity of human X-linked genes, a series of mouse-human somatic cell hybrids und...

journal_title：Nucleic acids research

authors： Brown CJ,Flenniken AM,Williams BR,Willard HF

更新日期：1990-07-25 00:00:00

abstract：:All exchangeable protons in a short DNA helix, d(CG)3 sodium salt, have been studied by proton nuclear magnetic resonance. The cytidine and guanosine amino protons have been assigned for the first time. As a function of temperature the cytidine amino protons and the imino protons behave very similarly, their relaxatio...

journal_title：Nucleic acids research

authors： Fazakerley GV,van der Marel GA,van Boom JH,Guschlbauer W

更新日期：1984-11-12 00:00:00

abstract：:As more and more high-throughput data has been produced by next-generation sequencing, it is still a challenge to classify RNA transcripts into protein-coding or non-coding, especially for poorly annotated species. We upgraded our original coding potential calculator, CNCI (Coding-Non-Coding Index), to CNIT (Coding-No...

journal_title：Nucleic acids research

authors： Guo JC,Fang SS,Wu Y,Zhang JH,Chen Y,Liu J,Wu B,Wu JR,Li EM,Xu LY,Sun L,Zhao Y

更新日期：2019-07-02 00:00:00

abstract：:MicA is a trans-encoded small non-coding RNA, which downregulates porin-expression in stationary-phase. In this work, we focus on the role of endoribonucleases III and E on Salmonella typhimurium sRNA MicA regulation. RNase III is shown to regulate MicA in a target-coupled way, while RNase E is responsible for the con...

journal_title：Nucleic acids research

authors： Viegas SC,Silva IJ,Saramago M,Domingues S,Arraiano CM

更新日期：2011-04-01 00:00:00

abstract：:Active DNA demethylation (ADDM) in mammals occurs via hydroxylation of 5-methylcytosine (5mC) by TET and/or deamination by AID/APOBEC family enzymes. The resulting 5mC derivatives are removed through the base excision repair (BER) pathway. At present, it is unclear how the cell manages to eliminate closely spaced 5mC ...

journal_title：Nucleic acids research

authors： Grin I,Ishchenko AA

更新日期：2016-05-05 00:00:00

abstract：:Struct2Net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. Prediction of protein-protein interactions (PPIs) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of t...

journal_title：Nucleic acids research

authors： Singh R,Park D,Xu J,Hosur R,Berger B

更新日期：2010-07-01 00:00:00

abstract：:Oriented DNA films prepared by the wet-spinning technique have been complexed with several ligands: the anthracycline antibiotic violamycin BI, the dipeptide L-carnosine, and the oligopeptide antibiotic netropsin. The formation of the DNA-ligand complexes is accompanied by dramatic changes of the conformational flexib...

journal_title：Nucleic acids research

authors： Fritzsche H,Rupprecht A,Richter M

更新日期：1984-12-11 00:00:00