ASTRAL compendium enhancements.

abstract：:Members of the basic helix-loop-helix (bHLH) family of transcription factors regulate a wide array of developmental processes in many cell types, including cell fate specification, differentiation and morphogenesis. Our studies describe the cloning of a gene from the nematode Caenorhabditis elegans that is closely rel...

journal_title：Nucleic acids research

authors： Nguyen L,Round J,O'Connell R,Geurts P,Funes-Duran M,Wong J,Jongeward G,Vierra CA

更新日期：2001-11-01 00:00:00

abstract：:Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections of MAR-containing vec...

journal_title：Nucleic acids research

authors： Grandjean M,Girod PA,Calabrese D,Kostyrko K,Wicht M,Yerly F,Mazza C,Beckmann JS,Martinet D,Mermod N

更新日期：2011-08-01 00:00:00

abstract：:Interferon regulatory factors IRF-3 and IRF-7 are transcription factors essential in the activation of interferon-β (IFN-β) gene in response to viral infections. Although, both proteins recognize the same consensus IRF binding site AANNGAAA, they have distinct DNA binding preferences for sites in vivo. The X-ray struc...

journal_title：Nucleic acids research

authors： De Ioannes P,Escalante CR,Aggarwal AK

更新日期：2011-09-01 00:00:00

abstract：:The mouse is widely used as system to study human genetic mechanisms. However, extensive rewiring of transcriptional regulatory networks often confounds translation of findings between human and mouse. Site-specific gain and loss of individual transcription factor binding sites (TFBS) has caused functional divergence ...

journal_title：Nucleic acids research

authors： Diehl AG,Boyle AP

更新日期：2018-02-28 00:00:00

abstract：:The k-junction is a structural motif in RNA comprising a three-way helical junction based upon kink turn (k-turn) architecture. A computer program written to examine relative helical orientation identified the three-way junction of the Arabidopsis TPP riboswitch as an elaborated k-turn. The Escherichia coli TPP ribosw...

journal_title：Nucleic acids research

authors： Wang J,Daldrop P,Huang L,Lilley DM

更新日期：2014-04-01 00:00:00

abstract：:Tubercidin may be directly brominated by reaction with N-bromosuccinimide in DMF to give 5-bromotubercidin, a reversible inhibitor of RNA synthesis. When buffered with potassium acetate the major product is 6-bromotubercidin. 5,6-Dibromotubercidin is formed in minor amounts under both conditions. N-Chlorosuccinimide a...

journal_title：Nucleic acids research

authors： Bergstrom DE,Brattesani AJ

更新日期：1980-12-20 00:00:00

abstract：:With the rapid accumulation of our knowledge on diseases, disease-related genes and drug targets, network-based analysis plays an increasingly important role in systems biology, systems pharmacology and translational science. The new release of VisANT aims to provide new functions to facilitate the convenient network ...

journal_title：Nucleic acids research

authors： Hu Z,Chang YC,Wang Y,Huang CL,Liu Y,Tian F,Granger B,Delisi C

更新日期：2013-07-01 00:00:00

abstract：:Constitutive expression of major histocompatibility complex class II (MHC II) antigens normally occurs in B-lymphocytes and antigen presenting cells of the monocyte/macrophage lineage. However, many malignant tumours and transformed cells express these proteins aberrantly. We demonstrate here that the MHC II DR alpha ...

journal_title：Nucleic acids research

authors： Cox PM,Goding CR

更新日期：1992-09-25 00:00:00

abstract：:Protein synthesis elongation factor 2 (EF-2) from all archaebacteria so far analysed, is susceptible to inactivation by diphtheria toxin, a property which it shares with EF-2 from the eukaryotic 8OS translation system. To resolve the structural basis of diphtheria toxin susceptibility, the structural gene for the EF-2...

journal_title：Nucleic acids research

authors： Lechner K,Heller G,Böck A

更新日期：1988-08-25 00:00:00

abstract：:Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor expressed in metazoan cells that is responsible for eliciting the production of type I interferons and pro-inflammatory cytokines upon detection of intracellular, non-self RNA. Structural studies of RIG-I have identified a novel Pincer domain com...

journal_title：Nucleic acids research

authors： Rawling DC,Kohlway AS,Luo D,Ding SC,Pyle AM

更新日期：2014-10-01 00:00:00

abstract：:We have constructed a complete, five-enzyme restriction map of the genome of the archaeon Halobacterium sp. GRB, based on a set of 84 overlapping cosmid clones. Fewer than 30 kbp, in three gaps, remain uncloned. The genome consists of five replicons: a chromosome (2038 kbp) and four plasmids (305, 90, 37, and 1.8 kbp)...

journal_title：Nucleic acids research

authors： St Jean A,Trieselmann BA,Charlebois RL

更新日期：1994-04-25 00:00:00

abstract：:The proportion of cytosines methylated in the DNA of nucleosome oligomers and of core particles appears indistinguishable from that of total nuclear DNA from CHO cells. However the DNA in nucleoprotein which is initially released from nuclei by treatment with very low levels of micrococcal nuclease and the first 10% o...

journal_title：Nucleic acids research

authors： Adams RL,McKay EL,Douglas JT,Burdon RH

更新日期：1977-09-01 00:00:00

abstract：:DNA synthesis is a fundamental requirement for cell proliferation and DNA repair, but no single method can identify the location, direction and speed of replication forks with high resolution. Mammalian cells have the ability to incorporate thymidine analogs along with the natural A, T, G and C bases during DNA synthe...

journal_title：Nucleic acids research

authors： Georgieva D,Liu Q,Wang K,Egli D

更新日期：2020-09-04 00:00:00

abstract：:One of the most intensively studied chromatin binding factors is HP1α. HP1α is associated with silenced, heterochromatic regions of the genome and binds to H3K9me3. While H3K9me3 is necessary for HP1α recruitment to heterochromatin, it is becoming apparent that it is not sufficient suggesting that additional factors a...

journal_title：Nucleic acids research

authors： Ryan DP,Tremethick DJ

更新日期：2018-10-12 00:00:00

abstract：:We have overproduced and purified wild type regA protein, a translational repressor encoded by bacteriophage T4. The repressor activity of the cloned regA protein has been tested on four known regA target genes (T4 genes: 44, 45, rpbA and regA) using in vitro coupled transcription-translation reactions. We have demons...

journal_title：Nucleic acids research

authors： Webster KR,Adari HY,Spicer EK

更新日期：1989-12-11 00:00:00

abstract：:The eel long interspersed element (LINE) UnaL2 and its partner short interspersed element (SINE) share a conserved 3' tail that is critical for their retrotransposition. The predicted secondary structure of the conserved 3' tail of UnaL2 RNA contains a stem region with a putative internal loop. Deletion of the putativ...

journal_title：Nucleic acids research

authors： Nomura Y,Kajikawa M,Baba S,Nakazato S,Imai T,Sakamoto T,Okada N,Kawai G

更新日期：2006-01-01 00:00:00

abstract：:The extent of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs was determined by infrared spectroscopy. From the infrared spectra taken at 20 degrees and 52 degrees C it is concluded that E. coli and B. stearothermophlius 5S RNAs possess a large number of base pairs (Table I). Comparison of our...

journal_title：Nucleic acids research

authors： Appel B,Erdmann VA,Stulz J,Ackerman T

更新日期：1979-10-25 00:00:00

abstract：:Translesion synthesis (TLS) employs low fidelity polymerases to replicate past damaged DNA in a potentially error-prone process. Regulatory mechanisms that prevent TLS-associated mutagenesis are unknown; however, our recent studies suggest that the PCNA-binding protein Spartan plays a role in suppression of damage-ind...

journal_title：Nucleic acids research

authors： Kim MS,Machida Y,Vashisht AA,Wohlschlegel JA,Pang YP,Machida YJ

更新日期：2013-02-01 00:00:00

abstract：:The multifunctional RNA-dependent RNA polymerase L protein of vesicular stomatitis virus catalyzes unconventional pre-mRNA capping via the covalent enzyme-pRNA intermediate formation, which requires the histidine-arginine (HR) motif in the polyribonucleotidyltransferase domain. Here, the effects of cap-defective mutat...

journal_title：Nucleic acids research

authors： Ogino T

更新日期：2014-10-29 00:00:00

abstract：:The RosettaDesign server identifies low energy amino acid sequences for target protein structures (http://rosettadesign.med.unc.edu). The client provides the backbone coordinates of the target structure and specifies which residues to design. The server returns to the client the sequences, coordinates and energies of ...

journal_title：Nucleic acids research

authors： Liu Y,Kuhlman B

更新日期：2006-07-01 00:00:00

abstract：:We have reported previously (1) two unexpected consequences of the microinjection into fertilized mouse eggs of a recombinant plasmid designated p12B1, carrying a 343 bp insert of non-repetitive mouse DNA. Injected at very low concentrations, this plasmid could be established as an extrachromosomal genetic element. Wh...

journal_title：Nucleic acids research

authors： Blangy A,Léopold P,Vidal F,Rassoulzadegan M,Cuzin F

更新日期：1991-12-01 00:00:00

abstract：:Members of the pentatricopeptide repeat domain (PPR) protein family bind RNA and are important for post-transcriptional control of organelle gene expression in unicellular eukaryotes, metazoans and plants. They also have a role in human pathology, as mutations in the leucine-rich PPR-containing (LRPPRC) gene cause sev...

journal_title：Nucleic acids research

authors： Baggio F,Bratic A,Mourier A,Kauppila TE,Tain LS,Kukat C,Habermann B,Partridge L,Larsson NG

更新日期：2014-12-16 00:00:00

abstract：:The human telomeric DNA sequence with four repeats can fold into a parallel-stranded propeller-type topology. NMR structures solved under molecular crowding experiments correlate with the crystal structures found with crystal-packing interactions that are effectively equivalent to molecular crowding. This topology has...

journal_title：Nucleic acids research

authors： Islam B,Sgobba M,Laughton C,Orozco M,Sponer J,Neidle S,Haider S

更新日期：2013-02-01 00:00:00

abstract：:The specific function of RNA molecules frequently resides in their seemingly unstructured loop regions. We performed a systematic analysis of RNA loops extracted from experimentally determined three-dimensional structures of RNA molecules. A comprehensive loop-structure data set was created and organized into distinct...

journal_title：Nucleic acids research

authors： Schudoma C,May P,Nikiforova V,Walther D

更新日期：2010-01-01 00:00:00

abstract：:Triantennary N-acetyl galactosamine (GalNAc, GN3: ), a high-affinity ligand for the hepatocyte-specific asialoglycoprotein receptor (ASGPR), enhances the potency of second-generation gapmer antisense oligonucleotides (ASOs) 6-10-fold in mouse liver. When combined with next-generation ASO designs comprised of short S-c...

journal_title：Nucleic acids research

authors： Prakash TP,Graham MJ,Yu J,Carty R,Low A,Chappell A,Schmidt K,Zhao C,Aghajan M,Murray HF,Riney S,Booten SL,Murray SF,Gaus H,Crosby J,Lima WF,Guo S,Monia BP,Swayze EE,Seth PP

更新日期：2014-07-01 00:00:00

abstract：:Synthesis and radioiodination of a stannyl oligodeoxyribonucleotide were undertaken to evaluate a gamma ray emitting ODN ligand for thrombus imaging in vivo . Synthesis of the ODN was based on modified automatedbeta-cyanoethyl phosphoramidite chemistry with an organotin nucleoside (dU*) coupled to a thrombin binding a...

journal_title：Nucleic acids research

authors： Dougan H,Hobbs JB,Weitz JI,Lyster DM

更新日期：1997-07-15 00:00:00

abstract：:KU is a heterodimer, consisting of approximately 70 and approximately 80 kDa subunits (KU70 and KU80, respectively), which is involved in a variety of nuclear functions. We generated tbKU80-deficient trypanosomes to explore the potential role of the tbKU complex in telomere maintenance and transcriptional regulation o...

journal_title：Nucleic acids research

authors： Janzen CJ,Lander F,Dreesen O,Cross GA

更新日期：2004-12-15 00:00:00

abstract：:We report thermodynamic values for binding of the guanosine nucleophile to the ribozyme derived from the Anabaena group I intron, and find that they are similar to those measured previously for the structurally distinct Tetrahymena ribozyme. The free energy of binding guanosine 5'-monophosphate (pG) at 30 degrees C is...

journal_title：Nucleic acids research

authors： Kuo LY,Cech TR

更新日期：1996-10-01 00:00:00

abstract：:An immunofluorescence assay was developed to identify proteins specifically binding to oligonucleoside phosphorodithioate (ODN) aptamers from a bead-bound ODN library. Accordingly, NF-kappaB p50 protein was incubated with either bead-bound NF-kappaB consensus sequence or a bead-bound ODN combinatorial library and adso...

journal_title：Nucleic acids research

authors： Yang X,Li X,Prow TW,Reece LM,Bassett SE,Luxon BA,Herzog NK,Aronson J,Shope RE,Leary JF,Gorenstein DG

更新日期：2003-05-15 00:00:00

abstract：:RNA sequencing has become an important method to perform hypothesis-free characterization of global gene expression. One of the limitations of RNA sequencing is that most sequence reads represent highly expressed transcripts, whereas low level transcripts are challenging to detect. To combine the benefits of tradition...

journal_title：Nucleic acids research

authors： Halvardson J,Zaghlool A,Feuk L

更新日期：2013-01-07 00:00:00