The interplay between H2A.Z and H3K9 methylation in regulating HP1α binding to linker histone-containing chromatin.

abstract：:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes an...

journal_title：Nucleic acids research

authors： Wang ML,Leitch AR,Schwarzacher T,Heslop-Harrison JS,Moore G

更新日期：1992-04-25 00:00:00

abstract：:A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtaine...

journal_title：Nucleic acids research

authors： Demidov V,Frank-Kamenetskii MD,Egholm M,Buchardt O,Nielsen PE

更新日期：1993-05-11 00:00:00

abstract：:NMR data from different experiments often contain errors; thus, automated backbone resonance assignment is a very challenging issue. In this paper, we present a method called GANA that uses a genetic algorithm to automatically perform backbone resonance assignment with a high degree of precision and recall. Precision ...

journal_title：Nucleic acids research

authors： Lin HN,Wu KP,Chang JM,Sung TY,Hsu WL

更新日期：2005-08-10 00:00:00

abstract：:We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium, allowing collaborative structural proteomics to be carried out in a distributed fashion. The core of SPINE is a laborato...

journal_title：Nucleic acids research

authors： Goh CS,Lan N,Echols N,Douglas SM,Milburn D,Bertone P,Xiao R,Ma LC,Zheng D,Wunderlich Z,Acton T,Montelione GT,Gerstein M

更新日期：2003-06-01 00:00:00

abstract：:Available evidence suggests that Mg2+ ions are involved in reactions catalyzed by hammerhead ribozymes. However, the activity in the presence of exclusively monovalent ions led us to question whether divalent metal ions really function as catalysts when they are present. We investigated ribozyme activity in the presen...

journal_title：Nucleic acids research

authors： Inoue A,Takagi Y,Taira K

更新日期：2004-08-09 00:00:00

abstract：:The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant wi...

journal_title：Nucleic acids research

authors： Sørensen PD,Frederiksen S

更新日期：1991-08-11 00:00:00

abstract：:A library of three synthetic ribozymes with randomized arms, targeting NUX, GUX and NXG triplets, respectively, were used to identify ribozyme-accessible sites on the HIV-1 LTR transcript comprising positions -533 to 386. Three cleavable sites were identified at positions 109, 115 and 161. Ribozymes were designed agai...

journal_title：Nucleic acids research

authors： Bramlage B,Luzi E,Eckstein F

更新日期：2000-11-01 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) Cmr (Rv1675c) is a CRP/FNR family transcription factor known to be responsive to cAMP levels and during macrophage infections. However, Cmr's DNA binding properties, cellular targets and overall role in tuberculosis (TB) complex bacteria have not been characterized. In this study, we u...

journal_title：Nucleic acids research

authors： Ranganathan S,Bai G,Lyubetskaya A,Knapp GS,Peterson MW,Gazdik M,C Gomes AL,Galagan JE,McDonough KA

更新日期：2016-01-08 00:00:00

abstract：:Periodically repeating DNA and protein elements are involved in various important biological events including genomic evolution, gene regulation, protein complex formation, and immunity. Notably, the currently used genome editing tools such as ZFNs, TALENs, and CRISPRs are also all associated with periodically repeati...

journal_title：Nucleic acids research

authors： Mori H,Evans-Yamamoto D,Ishiguro S,Tomita M,Yachie N

更新日期：2019-01-25 00:00:00

abstract：:Transcription factors (TFs) are sequence-specific DNA-binding proteins acting as critical regulators of gene expression. The Plant Promoter Analysis Navigator (PlantPAN; http://PlantPAN2.itps.ncku.edu.tw) provides an informative resource for detecting transcription factor binding sites (TFBSs), corresponding TFs, and ...

journal_title：Nucleic acids research

authors： Chow CN,Zheng HQ,Wu NY,Chien CH,Huang HD,Lee TY,Chiang-Hsieh YF,Hou PF,Yang TY,Chang WC

更新日期：2016-01-04 00:00:00

abstract：:Translation initiation in eukaryotes is mediated by assembly of the eIF4F complex over the m(7)GTP cap structure at the 5'-end of mRNAs. This requires an interaction between eIF4E and eIF4G, two eIF4F subunits. The Leishmania orthologs of eIF4E are structurally diverged from their higher eukaryote counterparts, since ...

journal_title：Nucleic acids research

authors： Yoffe Y,Léger M,Zinoviev A,Zuberek J,Darzynkiewicz E,Wagner G,Shapira M

更新日期：2009-06-01 00:00:00

abstract：:Holliday junctions (four-way DNA junctions), formed during homologous recombination, are bound and resolved by junction-specific endonucleases to yield recombinant duplex DNA products. The junction-resolving enzymes are a structurally diverse class of proteins that nevertheless have many properties in common; in parti...

journal_title：Nucleic acids research

authors： White MF,Lilley DM

更新日期：1998-12-15 00:00:00

abstract：:Ribosomal protein (rp)S5 belongs to the family of the highly conserved rp's that contains rpS7 from prokaryotes and rpS5 from eukaryotes. Alignment of rpS5/rpS7 from metazoans (Homo sapiens), fungi (Saccharomyces cerevisiae) and bacteria (Escherichia coli) shows that the proteins contain a conserved central/C-terminal...

journal_title：Nucleic acids research

authors： Lumsden T,Bentley AA,Beutler W,Ghosh A,Galkin O,Komar AA

更新日期：2010-03-01 00:00:00

abstract：:Although secondary structure predictions of an individual RNA sequence have been widely used in a number of sequence analyses of RNAs, accuracy is still limited. Recently, we proposed a method (called 'CentroidHomfold'), which includes information about homologous sequences into the prediction of the secondary structu...

journal_title：Nucleic acids research

authors： Hamada M,Yamada K,Sato K,Frith MC,Asai K

更新日期：2011-07-01 00:00:00

abstract：:Transcription of the bacteriophage Mu mom operon requires transactivation by the phage-encoded C protein. DNase I footprinting showed that in the absence of C, Escherichia coli RNA polymerase E(sigma)70 (RNAP) binds to the mom promoter (Pmom) region at a site, P2 (from -64 to -11 with respect to the transcription star...

journal_title：Nucleic acids research

authors： Sun W,Hattman S

更新日期：1996-10-15 00:00:00

abstract：:In the brome mosaic virus (BMV) virion, the coat protein (CP) selectively contacts the RNA motifs that regulate translation and RNA replication (Hoover et al., 2016. J. Virol. 90, 7748). We hypothesize that the unstructured N-terminal arm (NTA) of the BMV CP can specifically recognize RNA motifs. Using ion mobility sp...

journal_title：Nucleic acids research

authors： Jacobs A,Hoover H,Smith E,Clemmer DE,Kim CH,Kao CC

更新日期：2018-01-09 00:00:00

abstract：:Escherichia coli leucyl-tRNA synthetase (LeuRS) aminoacylates up to six different class II tRNA(leu) molecules. Each has a distinct anticodon and varied nucleotides in other regions of the tRNA. Attempts to construct a minihelix RNA that can be aminoacylated with leucine have been unsuccessful. Herein, we describe the...

journal_title：Nucleic acids research

authors： Larkin DC,Williams AM,Martinis SA,Fox GE

更新日期：2002-05-15 00:00:00

abstract：:We have reported on the differences in site-specific cleavage between DNA and DNA-RNA hybrids by various prototypic DNA cleavers (accompanying paper). In the case of bleomycin (BLM), degradation at 5'-GC-3'sites was suppressed relative to the same sequence in double-stranded DNA, while 5'-GT-3' damage remained constan...

journal_title：Nucleic acids research

authors： Bansal M,Stubbe J,Kozarich JW

更新日期：1997-05-01 00:00:00

abstract：:A simple, reproducible affinity chromatography method has been adapted for separation of high molecular weight supercoiled circular molecules from mammalian cells. Electron microscopic analysis of EB viral DNA obtained by this method, from the non-producer Burkitt's lymphoma line Raji, revealed monomer-sized viral mol...

journal_title：Nucleic acids research

authors： Kinchington D,Griffin BE

更新日期：1987-12-23 00:00:00

abstract：:Multiple myeloma, the second most frequent hematologic tumor after lymphomas, is an incurable cancer. Recent sequencing efforts have identified the ribonuclease DIS3 as one of the most frequently mutated genes in this disease. DIS3 represents the catalytic subunit of the exosome, a macromolecular complex central to th...

journal_title：Nucleic acids research

authors： Segalla S,Pivetti S,Todoerti K,Chudzik MA,Giuliani EC,Lazzaro F,Volta V,Lazarevic D,Musco G,Muzi-Falconi M,Neri A,Biffo S,Tonon G

更新日期：2015-05-26 00:00:00

abstract：:No analogous nucleoside triphosphate was found which acts as well as ATP in binding to and supporting catalysis of leucyl-tRNA synthetase from Escherichia coli MRE 600. However, there are numerous nucleotides which are able to replace ATP, but with lower efficiency. The 6-amino group of the adenine ring and the 2'-hyd...

journal_title：Nucleic acids research

authors： Marutzky R,Flossdorf J,Kula MR

更新日期：1976-08-01 00:00:00

abstract：:The European Nucleotide Archive (ENA; https://www.ebi.ac.uk/ena), provided by the European Molecular Biology Laboratory's European Bioinformatics Institute (EMBL-EBI), has for almost forty years continued in its mission to freely archive and present the world's public sequencing data for the benefit of the entire scie...

journal_title：Nucleic acids research

authors： Harrison PW,Ahamed A,Aslam R,Alako BTF,Burgin J,Buso N,Courtot M,Fan J,Gupta D,Haseeb M,Holt S,Ibrahim T,Ivanov E,Jayathilaka S,Balavenkataraman Kadhirvelu V,Kumar M,Lopez R,Kay S,Leinonen R,Liu X,O'Cathail C,Pa

更新日期：2021-01-08 00:00:00

abstract：:A short histone-like fusion RNA, generated when the RNA 3' processing signal from a mouse histone H4 gene is inserted into a heterologous transcription unit, becomes correctly down-regulated in G1-arrested cells of a temperature-sensitive mouse mastocytoma cell cycle mutant (21-Tb; Stauber et al., EMBO J. 5, 3297-3303...

journal_title：Nucleic acids research

authors： Stauber C,Schümperli D

更新日期：1988-10-25 00:00:00

abstract：:Recurrent chromosome aberrations are an important resource when associating human pathologies to specific genes. However, for technical reasons a large number of chromosome breakpoints are defined only at the level of cytobands and many of the genes involved remain unidentified. We developed a web-based information sy...

journal_title：Nucleic acids research

authors： Hoffmann R,Dopazo J,Cigudosa JC,Valencia A

更新日期：2005-01-01 00:00:00

abstract：:Minigenes encoding the peptide Met-Arg-Arg have been used to study the mechanism of toxicity of AGA codons proximal to the start codon or prior to the termination codon in bacteria. The codon sequences of the 'mini-ORFs' employed were initiator, combinations of AGA and CGA, and terminator. Both, AGA and CGA are low-us...

journal_title：Nucleic acids research

authors： Cruz-Vera LR,Magos-Castro MA,Zamora-Romo E,Guarneros G

更新日期：2004-08-18 00:00:00

abstract：:Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...

journal_title：Nucleic acids research

authors： Goldman-Levi R,Miller C,Bogoch J,Zak NB

更新日期：1996-08-15 00:00:00

abstract：:The cnjC gene from the protozoan Tetrahymena thermophila was completely sequenced. The deduced gene product was found to have significant sequence similarity to the yeast and prokaryotic RNA polymerase subunits involved with subunit assembly. Since cnjC is active only during the sexual stage (conjugation) of Tetrahyme...

journal_title：Nucleic acids research

authors： Martindale DW

更新日期：1990-05-25 00:00:00

abstract：:Proper cytokine gene expression is essential in development, homeostasis and immune responses. Studies on the transcriptional control of cytokine genes have mostly focused on highly researched transcription factors (TFs) and cytokines, resulting in an incomplete portrait of cytokine gene regulation. Here, we used enha...

journal_title：Nucleic acids research

authors： Santoso CS,Li Z,Lal S,Yuan S,Gan KA,Agosto LM,Liu X,Pro SC,Sewell JA,Henderson A,Atianand MK,Fuxman Bass JI

更新日期：2020-12-02 00:00:00

abstract：:To organize data resulting from the phenotypic characterization of a library of 30,000 T-DNA enhancer trap (ET) insertion lines of rice (Oryza sativa L cv. Nipponbare), we developed the Oryza Tag Line (OTL) database (http://urgi.versailles.inra.fr/OryzaTagLine/). OTL structure facilitates forward genetic search for sp...

journal_title：Nucleic acids research

authors： Larmande P,Gay C,Lorieux M,Périn C,Bouniol M,Droc G,Sallaud C,Perez P,Barnola I,Biderre-Petit C,Martin J,Morel JB,Johnson AA,Bourgis F,Ghesquière A,Ruiz M,Courtois B,Guiderdoni E

更新日期：2008-01-01 00:00:00

abstract：:Homeodomains appear to be one of the most frequently employed DNA-binding domains in a superfamily of transacting factors. It is likely that during evolution several sub-types of homeodomain have evolved from a common ancestral domain, resulting in distinct but closely related DNA-binding preferences. Here we describe...

journal_title：Nucleic acids research

authors： Jagla K,Stanceva I,Dretzen G,Bellard F,Bellard M

更新日期：1994-04-11 00:00:00