Abstract：
:A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtained. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site is targeted, and additionally enhanced if this site is in trans rather than in cis orientation. Thus in effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Demidov V,Frank-Kamenetskii MD,Egholm M,Buchardt O,Nielsen PEdoi
10.1093/nar/21.9.2103subject
Has Abstractpub_date
1993-05-11 00:00:00pages
2103-7issue
9eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract：:In mammalian cells, DNA damage induces robust changes in gene expression and these changes contribute to the proper execution of cellular responses to DNA damage, including DNA repair, cell cycle arrest and apoptosis. The transcription factor E2F-1 has been suggested to play a key role in the regulation of cell cycle-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.2.491
更新日期：1999-01-15 00:00:00
abstract：:A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNAf binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.20.6501
更新日期：1982-10-25 00:00:00
abstract：:DNA curvature plays a well-characterized role in many transcriptional regulation mechanisms. We present evidence for the conservation of curvature signals in putative regulatory regions of several archaeal and eubacterial genomes. Genes with highly curved upstream regions were identified in orthologous groups, based o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg882
更新日期：2003-12-01 00:00:00
abstract：:The replication origin region of the Escherichia coli K-12 chromosome has been cloned, and a region of 245 base-pairs has been shown to contain all the information for autonomous replication (defined ori). In order to obtain further information on the sequence organization in the defined ori stretch, various types of ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.12.3745
更新日期：1982-06-25 00:00:00
abstract：:Current literature describes several methods for the design of efficient siRNAs with 19 perfectly matched base pairs and 2 nt overhangs. Using four independent databases totaling 3336 experimentally verified siRNAs, we compared how well several of these methods predict siRNA cleavage efficiency. According to receiver ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm088
更新日期：2007-01-01 00:00:00
abstract：:The determination of distances between specific points in nucleic acids is essential to understanding their behaviour at the molecular level. The ability to measure distances of 2-10 nm is particularly important: deformations arising from protein binding commonly fall within this range, but the reliable measurement of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa086
更新日期：2020-04-06 00:00:00
abstract：:The GeneNet system is designed for collection and analysis of the data on gene and metabolic networks, signal transduction pathways and kinetic characteristics of elementary processes. In the past 2 years, the GeneNet structure was considerably improved: (i) the current version of the database is now implemented using...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki077
更新日期：2005-01-01 00:00:00
abstract：:Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.9.4011
更新日期：1984-05-11 00:00:00
abstract：:A novel resource centre for TP53 mutations and mutants has been developed (http://p53.fr). TP53 gene dysfunction can be found in the majority of human cancer types. The potential use of TP53 mutation as a biomarker for clinical studies or exposome analysis has led to the publication of thousands of reports describing ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1033
更新日期：2013-01-01 00:00:00
abstract：:MLL, the trithorax ortholog, is a well-characterized histone 3 lysine 4 methyltransferase that is crucial for proper regulation of the Hox genes during embryonic development. Chromosomal translocations, disrupting the Mll gene, lead to aggressive leukemia with poor prognosis. However, the functions of MLL in cellular ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku458
更新日期：2014-07-01 00:00:00
abstract：:During DNA double-strand break and replication fork repair by homologous recombination, the RAD51 recombinase catalyzes the DNA strand exchange reaction via a helical polymer assembled on single-stranded DNA, termed the presynaptic filament. Our published work has demonstrated a dual function of the SWI5-SFR1 complex ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw375
更新日期：2016-07-27 00:00:00
abstract：:In vivo footprinting experiments, augmented with gel shift and transfection analyses suggest that activation of the CYP1A1 gene by aryl hydrocarbons may be a multicomponent process. During the first 30 minutes of exposure to aryl hydrocarbon carcinogens and environmental contaminants, in vivo footprints appear at nine...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.9.1741
更新日期：1994-05-11 00:00:00
abstract：:Programmed -1 ribosomal frameshifting is employed in the expression of a number of viral and cellular genes. In this process, the ribosome slips backwards by a single nucleotide and continues translation of an overlapping reading frame, generating a fusion protein. Frameshifting signals comprise a heptanucleotide slip...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks629
更新日期：2012-09-01 00:00:00
abstract：:During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw302
更新日期：2016-07-08 00:00:00
abstract：:Genome-wide association studies (GWAS) have become a preferred method to identify new genetic susceptibility loci. This technique aims to understanding the molecular etiology of common diseases, but in many cases, it has led to the identification of loci with no obvious biological relevance. Herein, we show that previ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks169
更新日期：2012-06-01 00:00:00
abstract：:Post-synthetic enzymatic hypermethylation of DNA was induced in hamster fibrosarcoma cells by the DNA synthesis inhibitors cytosine arabinoside, hydroxyurea and aphidicolin. This effect required direct inhibition of DNA polymerase alpha or reduction in deoxynucleotide pools and was not specific to a single cell type. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.10.4353
更新日期：1986-05-27 00:00:00
abstract：:In the filamentous cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating the FurA-regulatory network, the number of target genes described for this essential transcription factor is limited to a handf...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku123
更新日期：2014-04-01 00:00:00
abstract：:The structure in solution of an RNA fragment (218 nucleotides long) containing part of E. coli 16S rRNA domain 2 has been studied using the intrinsic photoaffinity probe 4-thiouridine (s4U). In vitro transcription with T7 polymerase, in the presence of s4U triphosphate yielded complete RNA molecules. An affinity elect...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.13.3653
更新日期：1991-07-11 00:00:00
abstract：:Gene promoters are enriched in guanine clusters that potentially fold into quadruplex structures. Such quadruplexes were implicated in the regulation of gene expression, plausibly by interacting with transcription factors. We showed previously that homodimers of the myogenic transcription factor MyoD bound in vitro mo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1208
更新日期：2010-04-01 00:00:00
abstract：:We have isolated and characterized DNA probes that detect homologies between the X and Y chromosomes. Clone St25 is derived from the q13-q22 region of the X chromosome and recognizes a 98% homologous sequence on the Y chromosome. Y specific fragments were present in DNAs from 5 Yq-individuals and from 4 out of 7 XX ma...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.15.5485
更新日期：1985-08-12 00:00:00
abstract：:IMGT, the international ImMunoGeneTics database (http://imgt.cines. fr:8104 ), is a high-quality integrated database specialising in Immunoglobulins (Ig), T cell Receptors (TcR) and Major Histocompatibility Complex (MHC) molecules of all vertebrate species, created in 1989 by Marie-Paule Lefranc, Université Montpellie...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.219
更新日期：2000-01-01 00:00:00
abstract：:We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent to hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must exist t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.14.2774
更新日期：1996-07-15 00:00:00
abstract：:The Sleeping Beauty (SB) transposon is a promising gene transfer vector that integrates nonspecifically into host cell genomes. Herein, we attempt to direct transposon integration into predetermined DNA sites by coupling a site-specific DNA-binding domain (DBD) to the SB transposase. We engineered fusion proteins comp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm089
更新日期：2007-01-01 00:00:00
abstract：:Nuclei from frozen calf thymus suspended in buffer were analyzed for metal content prior to and after repeated washing. After three such extractions about 0.1 micrograms Zn/mg DNA and 0.025 micrograms Cu/mg DNA remained tightly associated with chromatin. This level of metal was essentially unchanged with subsequent wa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.21.5811
更新日期：1981-11-11 00:00:00
abstract：:One of the major issues in expression profiling analysis still is to outline proper thresholds to determine differential expression, while avoiding false positives. The problem being that the variance is inversely proportional to the log of signal intensities. Aiming to solve this issue, we describe a model, expressio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm093
更新日期：2007-01-01 00:00:00
abstract：:In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.16.3327
更新日期：2001-08-15 00:00:00
abstract：:KUPS (The University of Kansas Proteomics Service) provides high-quality protein-protein interaction (PPI) data for researchers developing and evaluating computational models for predicting PPIs by allowing users to construct ready-to-use data sets of interacting protein pairs (IPPs), non-interacting protein pairs (NI...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq943
更新日期：2011-01-01 00:00:00
abstract：:We have investigated the mechanism of the electrophoresis-driven chromatin aggregation which had been described by Weintraub (1984, Cell 38, 17-27) as a putative mean for propagation of genetic repression in eukaryotes. We show that the oligonucleosome aggregates are assembled de novo at the starting zone of DNP elect...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.24.7397
更新日期：1990-12-25 00:00:00
abstract：:Restriction fragment patterns of G+C-rich satellites of sheep and goat DNA were compared. The 1,712 g/cm3 satellites of both species appear homologous, consisting of repeats 760 base pairs long and showing coincidence of position of primary+ EcoRI, BamHI and most BspRI restriction target sites. The EcoRI and BamHI end...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.1.57
更新日期：1979-01-01 00:00:00
abstract：:Ribosomal protein L11 and its associated binding site on 23S rRNA together comprise one of the principle components that mediate interactions of translation factors with the ribosome. This site is also the target of the antibiotic thiostrepton, which has been proposed to act by preventing important structural transiti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh644
更新日期：2004-06-15 00:00:00
