Abstract：
:Post-synthetic enzymatic hypermethylation of DNA was induced in hamster fibrosarcoma cells by the DNA synthesis inhibitors cytosine arabinoside, hydroxyurea and aphidicolin. This effect required direct inhibition of DNA polymerase alpha or reduction in deoxynucleotide pools and was not specific to a single cell type. At equivalently reduced levels of DNA synthesis, neither cycloheximide, actinomycin D nor serum deprivation affected DNA methylation in this way. The topoisomerase inhibitors nalidixic acid and novobiocin caused significant hypomethylation indicating that increased 5-mCyt content was not a necessary consequence of DNA synthesis inhibition. The induced hypermethylation occurred predominantly in that fraction of the DNA synthesized in the presence of inhibitor; was stable in the absence of drug; was most prominent in low molecular weight DNA representing sites of initiated but incomplete DNA synthesis; and occurred primarily within CpG dinucleotides, although other dinucleotides were overmethylated as well. Drug-induced CpG hypermethylation may be capable of silencing genes, an effect which may be relevant to the aberrantly expressed genes characteristic of neoplastic cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Nyce J,Liu L,Jones PAdoi
10.1093/nar/14.10.4353subject
Has Abstractpub_date
1986-05-27 00:00:00pages
4353-67issue
10eissn
0305-1048issn
1362-4962journal_volume
14pub_type
杂志文章abstract：:The Superimposé webserver performs structural similarity searches with a preference towards 3D structure-based methods. Similarities can be detected between small molecules (e.g. drugs), parts of large structures (e.g. binding sites of proteins) and entire proteins. For this purpose, a number of algorithms were implem...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn285
更新日期：2008-07-01 00:00:00
abstract：:Prostatic steroid binding protein, whose expression is stimulated by androgens, consists of two subunits: one containing the polypeptides C1 and C3 and the other containing C2 and C3. We have characterised genomic clones containing the C1 and C2 genes by restriction enzyme analysis and DNA sequencing. Both genes are 3...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.17.5121
更新日期：1982-09-11 00:00:00
abstract：:Recent data showed that p53 stimulates the expression of genes encoding not only pro- but also antioxidant enzymes. It was suggested that antioxidant genes could be induced under physiologic levels of stress while the prooxidant ones respond to higher level of stress. Results presented in this article illustrate an ad...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm824
更新日期：2007-01-01 00:00:00
abstract：:Nucleotide excision repair (NER) is a major DNA repair pathway for a variety of DNA lesions. XPB plays a key role in DNA opening at damage sites and coordinating damage incision by nucleases. XPB is conserved from archaea to human. In archaea, XPB is associated with a nuclease Bax1. Here we report crystal structures o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa324
更新日期：2020-06-19 00:00:00
abstract：:The Eukaryotic Promoter Database (EPD) is an annotated non-redundant collection of eukaryotic POL II promoters, experimentally defined by a transcription start site (TSS). Access to promoter sequences is provided by pointers to positions in the corresponding genomes. Promoter evidence comes from conventional TSS mappi...
journal_title：Nucleic acids research
pub_type： 历史文章,杂志文章
doi：10.1093/nar/gkj146
更新日期：2006-01-01 00:00:00
abstract：:Restriction endonucleases are highly specific in recognizing the particular DNA sequence they act on. However, their activity is affected by sequence context, enzyme concentration and buffer composition. Changes in these factors may lead to either ineffective cleavage at the cognate restriction site or relaxed specifi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt257
更新日期：2013-06-01 00:00:00
abstract：:The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.3.332
更新日期：1994-02-11 00:00:00
abstract：:The human thrombopoietin (TPO) gene, which codes for the principal cytokine involved in platelet maturation, shows a peculiar alternative splicing of its last exon, where an intra-exonic 116 nt alternative intron is spliced out in a fraction of its mRNA. To characterize the molecular mechanism underlying this alternat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.4.886
更新日期：2001-02-15 00:00:00
abstract：:High-throughput experimental technologies gradually shift the paradigm of biological research from hypothesis-validation toward hypothesis-generation science. Translating diverse types of large-scale experimental data into testable hypotheses, however, remains a daunting task. We previously demonstrated that heterogen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku367
更新日期：2014-07-01 00:00:00
abstract：:Mitochondrial proteins remain the subject of intense research interest due to their implication in an increasing number of different conditions including mitochondrial and metabolic disease, cancer, and neuromuscular degenerative and age-related disorders. However, the mitochondrial proteome has yet to be accurately a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1001
更新日期：2016-01-04 00:00:00
abstract：:Neph et al. (2012) (Circuitry and dynamics of human transcription factor regulatory networks. Cell, 150: 1274-1286) reported the transcription factor (TF) regulatory networks of 41 human cell types using the DNaseI footprinting technique. This provides a valuable resource for uncovering regulation principles in differ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku923
更新日期：2014-11-10 00:00:00
abstract：:We have isolated a human cDNA (kup), encoding a new protein with two distantly spaced zinc fingers of the C2H2 type. This gene is highly conserved in mammals and is expressed mainly in hematopoietic cells and testis. Its expression was not higher in the various transformed cells tested than in the normal corresponding...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.7.1431
更新日期：1991-04-11 00:00:00
abstract：:Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.22.6209
更新日期：1991-11-25 00:00:00
abstract：:A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. The number of co-expressed proteins is not limited. Five 'pQLink' vectors for expression of His-tag and GST-tag fusion proteins as well as untagged proteins and for cloning by restriction en...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm067
更新日期：2007-01-01 00:00:00
abstract：:The genetic organization of the multiple ribosomal transcription units (RTUs) on the genome of the yeast Saccharomyces carlsbergensis was studied by electron microscopy of purified ribosomal DNA hybridized to 26S rRNA using the R-loop technique (Thomas, M., White, R.L. and Davis, R.W. (1973) Proc. Natl. Acad. Sci. U.S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.8.2801
更新日期：1978-08-01 00:00:00
abstract：:ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F(0)F(...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg326
更新日期：2003-05-01 00:00:00
abstract：:Chelation of divalent cations by 5 mM EDTA and subsequent removal by dialysis from a cytoplasmic HeLa cell extract leads to a complete loss of 5S rRNA transcription without affecting tRNA synthesis. Transcription complexes for 5S RNA can no longer be assembled in such a zinc-depleted extract and this ability can be fu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.23.8971
更新日期：1984-12-11 00:00:00
abstract：:The initiation region of the MS2 replicase cistron can be isolated as a fragment 59 bases in length protected from RNAase by the binding of the coat protein which serves as a translational repressor. This fragment MS2 R(-53 leads to 6) starts 53 bases before the initiation codon and retains full activity in binding ri...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.5.1761
更新日期：1979-01-01 00:00:00
abstract：:Multiple myeloma, the second most frequent hematologic tumor after lymphomas, is an incurable cancer. Recent sequencing efforts have identified the ribonuclease DIS3 as one of the most frequently mutated genes in this disease. DIS3 represents the catalytic subunit of the exosome, a macromolecular complex central to th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv387
更新日期：2015-05-26 00:00:00
abstract：:Synthesis of 8-phosphorus substituted isosteres of purine [pyrimidino (4,5-d)-1,3,2-diazaphosphole], 1-deazapurine [pyridino (2,3-d)-1,3,2-diazaphosphole] and 3-deazapurine [pyridino (4,5-d)-1,3,2-diazaphosphole] has been achieved by the reaction of equimolar amounts of triphenylphosphite and 4,5-diaminopyrimidine, 2,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.1.251
更新日期：1979-09-11 00:00:00
abstract：:The nucleoplasm is not homogenous; it consists of many types of nuclear bodies, also known as nuclear domains or nuclear subcompartments. These self-organizing structures gather machinery involved in various nuclear activities. Nuclear speckles (NSs) or splicing speckles, also called interchromatin granule clusters, w...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkx759
更新日期：2017-10-13 00:00:00
abstract：:The 2'-5' oligoadenylate synthetases form a well conserved family of interferon induced proteins, presumably present throughout the mammalian class. Using the Expressed Sequence Tag databases, we have identified a novel member of this family. This protein, which we named p59 2'-5' oligoadenylate synthetase-like protei...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.18.4121
更新日期：1998-09-15 00:00:00
abstract：:A complete physical map of the spinach mitochondrial genome has been established. The entire sequence content of 327 kilobase pairs (kb) is postulated to occur as a single circular molecule. Two directly repeated elements of approximately 6 kb, located on this "master chromosome", are proposed to participate in an int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.14.5651
更新日期：1986-07-25 00:00:00
abstract：:The sequential arrangement of histones along DNA in nucleosome core particles was determined between 0.5 and 600 mM salt and from 0 to 8 M urea. These concentrations of salt and urea up to 6 M had no significant effect on the linear order of histones along DNA but 8 M urea caused the rearrangement of histones. Conform...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.5.1053
更新日期：1981-03-11 00:00:00
abstract：:DNA dodecamers have been designed with two cytosines on each end and intervening A and T stretches, such that the oligomers have fully complementary A:T base pairs when aligned in the parallel orientation. Spectroscopic (UV, CD and IR), NMR and molecular dynamics studies have shown that oligomers having the sequences ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.7.1500
更新日期：2002-04-01 00:00:00
abstract：:The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.4.e20
更新日期：2001-02-15 00:00:00
abstract：:In order to systematically analyze the effects of nucleoside modification of sugar moieties in DNA polymerase reactions, we synthesized 16 modified templates containing 2',4'-bridged nucleotides and three types of 2',4'-bridged nucleoside-5'-triphospates with different bridging structures. Among the five types of ther...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn404
更新日期：2008-08-01 00:00:00
abstract：:Uracil-Specific Exision Reagent (USER) fusion is a recently developed technique that allows for assembly of multiple DNA fragments in a few simple steps. However, designing primers for USER fusion is both tedious and time consuming. Here, we present the Primer Help for USER (PHUSER) software, a novel tool for designin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr394
更新日期：2011-07-01 00:00:00
abstract：:Triple helix-forming oligonucleotides covalently linked to topoisomerase I inhibitors, in particular the antitumor agent camptothecin, trigger topoisomerase I-mediated DNA cleavage selectively in the proximity of the binding site of the oligonucleotide vector. In the present study, we have performed a systematic analy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg457
更新日期：2003-07-15 00:00:00
abstract：:Three-dimensional protein structures provide invaluable information for understanding and regulating biological functions of proteins. The GalaxyWEB server predicts protein structure from sequence by template-based modeling and refines loop or terminus regions by ab initio modeling. This web server is based on the met...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks493
更新日期：2012-07-01 00:00:00
