p59OASL, a 2'-5' oligoadenylate synthetase like protein: a novel human gene related to the 2'-5' oligoadenylate synthetase family.

abstract：:CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems allow bacteria to adapt to infection by acquiring 'spacer' sequences from invader DNA into genomic CRISPR loci. Cas proteins use RNAs derived from these loci to target cognate sequences for destruction through CRISPR inter...

journal_title：Nucleic acids research

authors： Xue C,Seetharam AS,Musharova O,Severinov K,Brouns SJ,Severin AJ,Sashital DG

更新日期：2015-12-15 00:00:00

abstract：:The chromatin structure of a germ-line specific gene, the TPG-3 gene, one of the rainbow trout protamine genes was analyzed in various tissues. The protamine genes are expressed in early stage testis but not in late stage testis, liver or erythrocyte. Five potential CpG methylation sites in the coding and flanking reg...

journal_title：Nucleic acids research

authors： Delcuve GP,Davie JR

更新日期：1987-04-24 00:00:00

abstract：:Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...

journal_title：Nucleic acids research

authors： Voltz K,Léonard J,Touceda PT,Conyard J,Chaker Z,Dejaegere A,Godet J,Mély Y,Haacke S,Stote RH

更新日期：2016-04-20 00:00:00

abstract：:The P0 scaffold protein of the ribosomal stalk is mainly incorporated into pre-ribosomes in the cytoplasm where it replaces the assembly factor Mrt4. In analyzing the role of the P0 carboxyl terminal domain (CTD) during ribosomal stalk assembly, we found that its complete removal yields a protein that is functionally ...

journal_title：Nucleic acids research

authors： Francisco-Velilla R,Remacha M,Ballesta JP

更新日期：2013-10-01 00:00:00

abstract：:We have investigated the substrate specificity of the Ogg1 protein of Saccharomyces cerevisiae (yOgg1 protein) for excision of modified DNA bases from oxidatively damaged DNA substrates using gas chromatography/isotope dilution mass spectrometry. Four DNA substrates prepared by treatment with H2O2/Fe(III)-EDTA/ascorbi...

journal_title：Nucleic acids research

authors： Karahalil B,Girard PM,Boiteux S,Dizdaroglu M

更新日期：1998-03-01 00:00:00

abstract：:Corynebacterium diphtheriae is a Gram-positive, non-spore forming, non-motile, pleomorphic rod belonging to the genus Corynebacterium and the actinomycete group of organisms. The organism produces a potent bacteriophage-encoded protein exotoxin, diphtheria toxin (DT), which causes the symptoms of diphtheria. This pote...

journal_title：Nucleic acids research

authors： Cerdeño-Tárraga AM,Efstratiou A,Dover LG,Holden MT,Pallen M,Bentley SD,Besra GS,Churcher C,James KD,De Zoysa A,Chillingworth T,Cronin A,Dowd L,Feltwell T,Hamlin N,Holroyd S,Jagels K,Moule S,Quail MA,Rabbinowitsch E

更新日期：2003-11-15 00:00:00

abstract：:ProtoNet is an automatic hierarchical classification of the protein sequence space. In 2004, the ProtoNet (version 4.0) presents the analysis of over one million proteins merged from SwissProt and TrEMBL databases. In addition to rich visualization and analysis tools to navigate the clustering hierarchy, we incorporat...

journal_title：Nucleic acids research

authors： Kaplan N,Sasson O,Inbar U,Friedlich M,Fromer M,Fleischer H,Portugaly E,Linial N,Linial M

更新日期：2005-01-01 00:00:00

abstract：:The structure of eukaryotic chromatin has been investigated by isolating and analyzing the "accessible" DNA fraction of rat liver chromatin. This DNA fraction has been isolated by titrating the chromatin with the protese-resistant D isomer of polylysine to bind the "accessible" DNA sites. After removal of chromosomal ...

journal_title：Nucleic acids research

authors： Arnold EA,Wahn U,Young KE

更新日期：1975-05-01 00:00:00

abstract：:Naturally occurring hammerhead ribozymes are produced by rolling circle replication followed by self-cleavage. This results in monomer-length catalytic RNAs which have self-complementary sequences that can occupy their trans -binding domains and potentially block their ability to cleave other RNA strands. Here we show...

journal_title：Nucleic acids research

authors： Ohmichi T,Kool ET

更新日期：2000-02-01 00:00:00

abstract：:Chromatin organization and epigenetic markers influence splicing, though the magnitudes of these effects and the mechanisms are largely unknown. Here, we demonstrate that linker histone H1.5 influences mRNA splicing. We observed that linker histone H1.5 binds DNA over splice sites of short exons in human lung fibrobla...

journal_title：Nucleic acids research

authors： Glaich O,Leader Y,Lev Maor G,Ast G

更新日期：2019-07-09 00:00:00

abstract：:The Diatom Expressed Sequence Tag (EST) Database was constructed to provide integral access to ESTs from these ecologically and evolutionarily interesting microalgae. It has now been updated with 130,000 Phaeodactylum tricornutum ESTs from 16 cDNA libraries and 77,000 Thalassiosira pseudonana ESTs from seven libraries...

journal_title：Nucleic acids research

authors： Maheswari U,Mock T,Armbrust EV,Bowler C

更新日期：2009-01-01 00:00:00

abstract：:A very mild and efficient procedure has been developed for the preparation of C-5 substituted deoxyuridines. The substituent carries a masked primary aliphatic amino group. These compounds are readily converted into their phosphoramidites and can be used to prepare oligonucleotides carrying one or more aliphatic amino...

journal_title：Nucleic acids research

authors： Haralambidis J,Chai M,Tregear GW

更新日期：1987-06-25 00:00:00

abstract：:Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...

journal_title：Nucleic acids research

authors： Serçinoglu O,Ozbek P

更新日期：2018-07-02 00:00:00

abstract：:Adenosine-to-inosine (A-to-I) RNA editing is a common post transcriptional modification. It has a critical role in protecting against false activation of innate immunity by endogenous double stranded RNAs and has been associated with various regulatory processes and diseases such as autoimmune and cardiovascular disea...

journal_title：Nucleic acids research

authors： Schaffer AA,Kopel E,Hendel A,Picardi E,Levanon EY,Eisenberg E

更新日期：2020-06-19 00:00:00

abstract：:Organisms growing at elevated temperatures face the challenge of maintaining the integrity of their genetic materials. Archaea possess unique chromatin proteins for gene organization and information processing. We present the solution structure of Sso7c4 from Sulfolobus solfataricus, which has a homodimeric DNA-bindin...

journal_title：Nucleic acids research

authors： Hsu CH,Wang AH

更新日期：2011-08-01 00:00:00

abstract：:With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targete...

journal_title：Nucleic acids research

authors： Schirmer M,Ijaz UZ,D'Amore R,Hall N,Sloan WT,Quince C

更新日期：2015-03-31 00:00:00

abstract：:The cause of 50S ribosomal subunit collapse reportedly triggered by hybridization of a 14-base cDNA probe to the alpha-sarcin region of 23S rRNA was investigated by physical measurement of probe-subunit complexes in varying buffer conditions. The results reported here show that this probe was unable to hybridize to it...

journal_title：Nucleic acids research

authors： White GA,Wood T,Hill WE

更新日期：1988-11-25 00:00:00

abstract：:Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material w...

journal_title：Nucleic acids research

authors： Frolova EI,Zalmanzon ES,Lukanidin EM,Georgiev GP

更新日期：1978-01-01 00:00:00

abstract：:The tumor suppressor p53 is a sequence-specific transcription factor, which regulates the expression of target genes involved in different stress responses. To understand p53's essential transcriptional functions, unbiased analysis of its DNA-binding repertoire is pivotal. In a genome-wide tiling ChIP-on-chip approach...

journal_title：Nucleic acids research

authors： Smeenk L,van Heeringen SJ,Koeppel M,van Driel MA,Bartels SJ,Akkers RC,Denissov S,Stunnenberg HG,Lohrum M

更新日期：2008-06-01 00:00:00

abstract：:We have studied the effect of temperature on transcription initiation in vitro at two related promoters ga/Pcon and ga/P1, which have the same nucleotide sequence around the -10 region and transcription start site, but differ in upstream sequences. One of the promoters, ga/Pcon, carries the consensus -35 hexamer, 5'TT...

journal_title：Nucleic acids research

authors： Grimes E,Busby S,Minchin S

更新日期：1991-11-25 00:00:00

abstract：:Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and th...

journal_title：Nucleic acids research

authors： Ghosh T,Soni K,Scaria V,Halimani M,Bhattacharjee C,Pillai B

更新日期：2008-11-01 00:00:00

abstract：:Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (sha...

journal_title：Nucleic acids research

authors： Chiu TP,Rao S,Mann RS,Honig B,Rohs R

更新日期：2017-12-01 00:00:00

abstract：:The expression of thymidine kinase in fungi, which normally lack this enzyme, will greatly aid the study of DNA metabolism and provide useful drug-sensitive phenotypes. The herpes simplex virus type-1 thymidine kinase gene ( tk ) was expressed in Neurospora crassa. tk was expressed as a fusion to N.crassa arg-2 regula...

journal_title：Nucleic acids research

authors： Sachs MS,Selker EU,Lin B,Roberts CJ,Luo Z,Vaught-Alexander D,Margolin BS

更新日期：1997-06-15 00:00:00

abstract：:The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chromophore. First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 nm are presented. The main Raman hands are assigned to the convenient nucleic bases by comparison with the sp...

journal_title：Nucleic acids research

authors： Chinsky L,Turpin PY

更新日期：1978-08-01 00:00:00

abstract：:A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces ...

journal_title：Nucleic acids research

authors： Yamashita A,Takayama T,Iwata R,Yamamoto M

更新日期：2013-11-01 00:00:00

abstract：:We report for the first time an analysis of the ATPase activity of human DNA topoisomerase (topo) IIbeta. We show that topo IIbeta is a DNA-dependent ATPase that appears to fit Michaelis-Menten kinetics. The ATPase activity is stimulated 44-fold by DNA. The k(cat) for ATP hydrolysis by human DNA topo IIbeta in the pre...

journal_title：Nucleic acids research

authors： West KL,Turnbull RM,Willmore E,Lakey JH,Austin CA

更新日期：2002-12-15 00:00:00

abstract：:Early in its differentiative pathway, a given B lymphocyte expresses immunoglobulin of the mu heavy chain class (IgM). Subsequent differentiative processes may involve rearrangement within the immunoglobulin heavy chain chromosomal locus to enable cells of the same lineage to synthesize immunoglobulins of other heavy ...

journal_title：Nucleic acids research

authors： Mowatt M,Dery C,Dunnick W

更新日期：1985-01-11 00:00:00

abstract：:The RecQL4 helicase is involved in the maintenance of genome integrity and DNA replication. Mutations in the human RecQL4 gene cause the Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. Mouse models and experiments in human and Xenopus have proven the N-terminal part of RecQL4 to be vital for cell growth. We ...

journal_title：Nucleic acids research

authors： Ohlenschläger O,Kuhnert A,Schneider A,Haumann S,Bellstedt P,Keller H,Saluz HP,Hortschansky P,Hänel F,Grosse F,Görlach M,Pospiech H

更新日期：2012-09-01 00:00:00

abstract：:Exome sequencing strategy is promising for finding novel mutations of human monogenic disorders. However, pinpointing the casual mutation in a small number of samples is still a big challenge. Here, we propose a three-level filtration and prioritization framework to identify the casual mutation(s) in exome sequencing ...

journal_title：Nucleic acids research

authors： Li MX,Gui HS,Kwan JS,Bao SY,Sham PC

更新日期：2012-04-01 00:00:00

abstract：:In Trypanosoma brucei, the mutually exclusive expression of the major surface antigens, the variant surface glycoprotein (VSG) of the bloodstream form and procyclin of the procyclic form, is due to a stage-specific accumulation of the respective mRNAs. Through the targeting of a reporter construct in the procyclin pro...

journal_title：Nucleic acids research

authors： Vanhamme L,Berberof M,Le Ray D,Pays E

更新日期：1995-06-11 00:00:00